Article
Immunology
Yuping Wang, Tengkai Wang, Meijuan Cai, Shuzhen Zhu, Lijun Song, Qian Wang
Summary: The study found that in patients with SLE, the expression of Fc epsilon RI alpha increased while the level of anti-IgE decreased. Additionally, in the CHO3D10 cell line, Fc epsilon RI alpha was found to exist in both monomer and various polymer forms. Mast cell-associated Fc epsilon RI alpha plays a role in the inflammatory lesions of SLE.
IMMUNITY INFLAMMATION AND DISEASE
(2022)
Article
Multidisciplinary Sciences
Donald MacGlashan
Summary: This study found that released FceRI-alpha accounted for 7% of total surface FceRI before the reaction, with a molecular weight of approximately 54 kD. The release of FceRI-alpha was found to be independent of internalized processing and starting density of FceRI, and was more effective with non-IgE-dependent stimuli.
Article
Biochemistry & Molecular Biology
Tsubasa Ashikari, Masakazu Hachisu, Kazuki Nagata, Daisuke Ando, Yuki Iizuka, Naoto Ito, Kandai Ito, Yuki Ikeda, Hiroki Matsubara, Takuya Yashiro, Kazumi Kasakura, Chiharu Nishiyama
Summary: Mast cells play a significant role in IgE-mediated immunoresponses, and salicylaldehyde, identified as an inhibitor of mast cell activation, shows potential as an antiallergic compound.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Sathisha Upparahalli Venkateshaiah, Madhavi Rayapudi, Hemanth Kumar Kandikattu, Chandra Sekhar Yadavalli, Anil Mishra
Summary: This study demonstrates that IL-15 responsive T cells and IgE receptors can serve as novel non-invasive biomarkers for distinguishing between EoE and GERD. Additionally, analysis of blood mRNA levels reveals certain receptors significantly reduced in EoE patients, which can be used as predictors for EoE.
CLINICAL IMMUNOLOGY
(2021)
Review
Cell Biology
Yuka Nagata, Ryo Suzuki
Summary: Mast cells (MCs) have different roles in allergic responses, and the interactions between antigens and immunoglobulin E (IgE) can trigger diverse responses in MCs. The clustering of high-affinity IgE receptor Fc epsilon RI is crucial for signal transduction and determines the quality of MC responses. Antigen properties precisely regulate Fc epsilon RI dynamics, which can result in different outcomes by switching the intracellular signaling pathway, suggesting that antigen properties can control MC responses both qualitatively and quantitatively.
Article
Allergy
Cynthia Kanagaratham, Tahereh Derakhshan, Yasmeen S. El Ansari, Kameryn N. Furiness, Eleanor Hollers, Mats Keldsen, Hans C. Oettgen, Daniel F. Dwyer
Summary: In this study, the inhibitory effects of IgG:Fc?RIIb signaling on MC responses to IgE were assessed using complementary transcriptomic and functional approaches. The results showed that IgG signaling via Fc?RIIb has a suppressive effect on IgE-induced MC transcription, especially genes related to bone marrow-derived MCs activation. These findings are important for understanding the regulatory mechanisms of IgG on IgE-induced MC responses.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Article
Critical Care Medicine
Angelica Tiotiu, Yusef Badi, Nazanin Zounemat Kermani, Marek Sanak, Johan Kolmert, Craig E. Wheelock, Philip M. Hansbro, Sven-Erik Dahlen, Peter J. Sterk, Ratko Djukanovic, Yike Guo, Sharon Mumby, Ian M. Adcock, Kian Fan Chung
Summary: By analyzing gene signatures of MC activation, we can identify phenotypes of severe asthma and indicate that MCs can have distinct transcriptional phenotypes associated with specific clinical phenotypes.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2022)
Article
Immunology
Mohammed Zghaebi, Maria Byazrova, Sabine Flicker, Sergio Villazala-Merino, Nicholas J. Campion, Victoria Stanek, Aldine Tu, Heimo Breiteneder, Alexander Filatov, Musa Khaitov, Verena Niederberger-Leppin, Julia Eckl-Dorna, Rudolf Valenta
Summary: A new sequential gating strategy was developed to detect cells bearing the IgE BCR on their surface, using a monoclonal anti-IgE antibody. This strategy allowed for the clear identification of allergen-specific IgE(+) memory B cells and plasma cells in human PBMCs, offering new insights into allergic responses.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Oncology
Mohammad Fereydouni, Mona Motaghed, Elnaz Ahani, Tal Kafri, Kristen Dellinger, Dean D. Metcalfe, Christopher L. Kepley
Summary: The role of mast cells in cancer pathologies is controversial, but they could serve as a platform for developing novel mechanisms of tumor immunotherapy.
FRONTIERS IN ONCOLOGY
(2022)
Review
Allergy
Luc Colas, Antoine Magnan, Sophie Brouard
Summary: This article summarizes the recent advances in the regulation, biology, and physiopathology of immunoglobulin E (IgE) beyond allergic diseases, opening up new fields of exploration in IgE biology.
Article
Biochemistry & Molecular Biology
Syed Benazir Alam, Ashley Wagner, Steven Willows, Marianna Kulka
Summary: Quercetin and resveratrol have differential inhibitory effects on mast cells, potentially through modulation of FcεRI expression, signaling, and mediator release.
Article
Allergy
Seong-Dae Woo, Eun-Mi Yang, Jaehyuk Jang, Youngsoo Lee, Yoo Seob Shin, Young-Min Ye, Su Youn Nam, Kyung Wha Lee, Myoung Ho Jang, Hae-Sim Park
Summary: Serum-free IgE levels are significantly higher in adults with asthma compared to healthy controls, and are closely associated with atopic status and type 2 asthma. It may serve as a useful biomarker for assessing asthma severity in adults with asthma.
ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY
(2021)
Article
Allergy
Shinya Imamura, Ken Washio, Mayuko Mizuno, Yoshiko Oda, Atsushi Fukunaga, Chikako Nishigori
Summary: This study found that basophils in patients with atopic dermatitis exhibited spontaneous activation and lower responsiveness to anti-IgE stimulation compared to healthy controls, despite elevated levels of serum IgE. Additionally, Fc epsilon RI expression on basophils was higher in patients with atopic dermatitis than in healthy controls, while surface-bound IgE was relatively low.
ALLERGOLOGY INTERNATIONAL
(2021)
Article
Immunology
Robert W. E. Crozier, Val A. Fajardo, Adam J. MacNeil
Summary: This study aimed to evaluate the potential therapeutic target of GSK3 in allergic inflammation. The results revealed that GSK3 inhibition reduced ROS production and degranulation, and decreased the production/release of pro-inflammatory mediators. Additionally, CHIR99021-mediated GSK3 inhibition altered the pro-inflammatory phenotype of mast cells.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Greer K. Arthur, Glenn Cruse
Summary: Mast cells are immune cells involved in allergic inflammatory response. This review focuses on IgE-mediated activation and discusses the structure and function of Fc epsilon RI, as well as the role of Fc epsilon RI beta in regulating mast cell function and signaling. Current and emerging approaches to target IgE and Fc epsilon RI signaling are discussed, along with the potential therapeutic use of alternative splicing of Fc epsilon RI beta.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mariarosaria Conte, Rosanna Palumbo, Alessandra Monti, Elisabetta Fontana, Angela Nebbioso, Menotti Ruvo, Lucia Altucci, Nunzianna Doti
Summary: The AIF/CypA complex has lethal activity in rodent models of acute brain injury, but an AIF peptide has been found to restore cell survival and prevent brain injury. This study shows that the peptide can also protect against apoptosis in PD cells, suggesting the AIF/CypA complex as a potential target for developing new PD therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Pasqualina Liana Scognamiglio, Caterina Vicidomini, Francesco Fontanella, Claudio De Stefano, Rosanna Palumbo, Giovanni N. Roviello
Summary: Benzofuran derivatives have potential as drugs due to their ability to interact with nucleic acids, interfere with amyloid peptide aggregation, and affect cancer cell cycles. This study investigated the binding ability of two benzofuran compounds (BF1 and BDF1) with bovine serum albumin as a model protein. The results showed that BF1 had a higher affinity for protein binding, leading to changes in protein structure and thermal stability compared to BDF1.
Article
Biology
Donata Molisso, Mariangela Coppola, Martina Buonanno, Ilaria Di Lelio, Simona Maria Monti, Chiara Melchiorre, Angela Amoresano, Giandomenico Corrado, John Paul Delano-Frier, Andrea Becchimanzi, Francesco Pennacchio, Rosa Rao
Summary: Prosystemin is not only a precursor of Systemin peptide, but also contains other biologically active regions. Experimental results show that truncated Prosystemin can promote defense barriers in tomato plants through a hormone-independent defense pathway, and provide protection against pests and pathogens.
Editorial Material
Biochemistry & Molecular Biology
Simona Maria Monti, Giuseppina De Simone, Emma Langella
Article
Pharmacology & Pharmacy
Giuseppa Augello, Antonina Azzolina, Filomena Rossi, Filippo Prencipe, Giuseppe Felice Mangiatordi, Michele Saviano, Luisa Ronga, Melchiorre Cervello, Diego Tesauro
Summary: Gold complexes have gained attention as non-platinum antitumor agents due to their strong antiproliferative effects via non-cisplatin-like mechanisms. Studies have shown that many cytotoxic gold compounds, such as N-heterocyclic carbene (NHC)-gold(I) complexes, inhibit thioredoxin reductase (TrxR) activity. In this study, two gold complexes, Au4BC and Au3BC, were tested on cancer cells. Au4BC inhibited cell viability and induced oxidative stress, DNA damage, autophagy, and apoptosis, while Au3BC only inhibited TrxR1 activity without affecting cell viabilities, and induced the up-regulation of RAD51 and p62 proteins involved in DNA damage repair and autophagy. Au3BC showed potential as an anticancer drug for HCC and BC, independent of TrxR1 inhibition.
Review
Biochemistry & Molecular Biology
Giuseppe Felice Mangiatordi, Maria Maddalena Cavalluzzi, Pietro Delre, Giuseppe Lamanna, Maria Cristina Lumuscio, Michele Saviano, Jean-Pierre Majoral, Serge Mignani, Andrea Duranti, Giovanni Lentini
Summary: The endocannabinoid system (ECS) plays a crucial role in various physiological and pharmacological processes, but the natural ligands responsible for its effects have a short half-life. This perspective explores the potential role of inhibitors targeting the enzymes FAAH and MGL, which degrade endogenous ligands, in psychiatric disorders and related pathologies. The study considers the impact of exogenous ligands and predicts the potential toxicity of inhibitors before clinical studies, specifically focusing on cardiotoxicity as an adverse reaction and identifying compounds that could be repurposed as oral antipsychotics.
Article
Biochemistry & Molecular Biology
Annamaria Sandomenico, Alessia Ruggiero, Emanuela Iaccarino, Angela Oliver, Flavia Squeglia, Miguel Moreira, Luciana Esposito, Menotti Ruvo, Rita Berisio
Summary: CD59 is an abundant protein in humans that protects cells from damage by inhibiting the complement system. It inhibits the assembly of the Membrane Attack Complex (MAC) and is incorporated by pathogenic viruses like HIV-1 to escape complement-mediated virolysis. CD59 is also overexpressed in cancer cells to resist complement attack. In this study, bioinformatics and computational tools were used to identify CD59 interactions and design paratope-mimicking peptides that target CD59.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Monica-Cornelia Sardaru, Narcisa-Laura Marangoci, Rosanna Palumbo, Giovanni N. Roviello, Alexandru Rotaru
Summary: In this article, recent advancements in fluorescent and Raman imaging using oligodeoxyribonucleotide (ODN)-based nanoparticles and nanostructures are reviewed, which show promise as effective tools for live-cell analysis. These nanodevices can be used to investigate a vast number of biological processes occurring at various levels, and have contributed to significant advancements in understanding the role of specific analytes in pathological processes and diagnosing health conditions. The literature reports predict that the development of ODN-based fluorescent and Raman probes will continue in the near future, leading to novel applications in therapeutic and diagnostic strategies.
Correction
Chemistry, Physical
Valentina Verdoliva, Michele Saviano, Stefania De Luca
Article
Biology
Emma Langella, Davide Esposito, Simona Maria Monti, Claudiu T. Supuran, Giuseppina De Simone, Vincenzo Alterio
Summary: Carbonic anhydrases are enzymes involved in an important physiological reaction of converting CO2 to bicarbonate ion. They play crucial roles in various physiological and pathological processes in humans, making them potential targets for therapeutic interventions. This study investigates a class of carbonic anhydrase inhibitors, providing insights into their mechanism of action and potential for drug design.
Article
Chemistry, Inorganic & Nuclear
Mikel Bernabeu de Maria, Diego Tesauro, Filippo Prencipe, Michele Saviano, Luigi Messori, Christine Enjalbal, Ryszard Lobinski, Luisa Ronga
Summary: Methylmercury, mercury (II), and mercury (I) chlorides were found to react with vasopressin and its mono- and diselenium analogues to form various mercury-peptide adducts. The replacement of Cys by SeCys in vasopressin increased reactivity towards methylmercury, resulting in the formation of -Se/S-Hg-Se-bridged structures and subsequent demethylation of methylmercury. Competitive experiments showed that CH3HgCl preferred to react with the diselenium analogue rather than vasopressin. The diselenium peptide also demonstrated the ability to displace CH3Hg moieties bound to S in vasopressin. These findings suggest the potential use of selenopeptides for methylmercury chelation and detoxification strategies.
INORGANIC CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Giuseppe Lamanna, Pietro Delre, Gilles Marcou, Michele Saviano, Alexandre Varnek, Dragos Horvath, Giuseppe Felice Mangiatordi
Summary: This study introduces a new de novo design algorithm called GENERA that combines the capabilities of a deep-learning algorithm for automated drug-like analogue design, called DeLA-Drug, with a genetic algorithm for generating molecules with desired target-oriented properties. GENERA was applied to the angiotensin-converting enzyme 2 (ACE2) target, and its ability to de novo design promising candidates was assessed using docking programs PLANTS and GLIDE. The study demonstrates that GENERA can effectively perform multiobjective optimization and generate focused libraries with better scores compared to a starting set of known ACE-2 binders.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Article
Chemistry, Medicinal
Jwala P. Sivaccumar, Emanuela Iaccarino, Angela Oliver, Maria Cantile, Pierpaolo Olimpieri, Antonio Leonardi, Menotti Ruvo, Annamaria Sandomenico
Summary: Drug development has shifted toward personalized treatments targeting specific therapeutic targets in individual patients. Monoclonal antibodies (mAbs) and their surrogates, such as Fabs, are increasingly used for their ability to bind and inhibit therapeutic targets. In this study, a recombinant humanized Fab of a murine mAb (3D1) was prepared and characterized, showing potential for targeting Nodal-positive cancer cells.
Article
Chemistry, Multidisciplinary
Pasqualina Liana Scognamiglio, Claudia Riccardi, Rosanna Palumbo, Thomas F. Gale, Domenica Musumeci, Giovanni N. Roviello
Summary: The design, synthesis and characterization of a novel nucleoamino acid derivative named TrpT were described. The self-assembly of TrpT into supramolecular networks in aqueous solution was demonstrated. TrpT nanoaggregates showed good stability, spherical-shaped assemblies, and the ability to bind curcumin. TrpT also interacted with the serum protein BSA and showed high stability in human serum.
JOURNAL OF NANOSTRUCTURE IN CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Anna Giorgio, Annarita Del Gatto, Simone Pennacchio, Michele Saviano, Laura Zaccaro
Summary: This review focuses on the applications of peptoids in the diagnostic field, covering the literature from the past decade regarding their use as early diagnostic tools for pathologies with a significant impact on human health and social behavior. The review provides insights into the peptoids' employment in targeted cancer imaging and blood-based screening of neurological and autoimmune diseases, emphasizing the need for continued research and support in this area due to their promising properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)