Article
Immunology
Kaveh Abdi, Karen Laky, Mehrnoosh Abshari, Elizabeth M. Hill, Larry Lantz, Nevil J. Singh, Eric O. Long
Summary: It has been found that NK cells produce IFN-gamma before the production of IL-12 by DCs in the early stage of immune response. Toll-like Receptor 4 (TLR4) on DCs and soluble factors produced by LPS-activated DCs play important roles in IFN-gamma production by NK cells.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Biology
Wei-Jia Luo, Sung-Liang Yu, Chia-Ching Chang, Min-Hui Chien, Ya-Ling Chang, Keng-Mao Liao, Pei-Chun Lin, Kuei-Pin Chung, Ya-Hui Chuang, Jeremy J. W. Chen, Pan-Chyr Yang, Kang-Yi Su
Summary: HLJ1 knockout mice show reduced organ injury and mortality in lipopolysaccharide-induced endotoxic shock. HLJ1 deletion affects IFN-gamma-related gene signatures in distinct immune cell clusters. HLJ1 deficiency leads to reduced IL-12 levels, dampened IFN-gamma production in natural killer cells, and improved survival rate. HLJ1 converts misfolded IL-12p35 homodimers to monomers, maintaining bioactive IL-12p70 heterodimerization and secretion.
Article
Oncology
Hemavathi Dhandapani, Hascitha Jayakumar, Abirami Seetharaman, Shirley Sunder Singh, Selvaluxmy Ganeshrajah, Nirmala Jagadish, Anil Suri, Rajkumar Thangarajan, Priya Ramanathan
Summary: Dendritic cell-based immunotherapy utilizing recombinant human SPAG9 shows promise in stimulating CD4(+) and CD8(+) T cell responses for cervical cancer patients. The study suggests rhSPAG9 as a strong immunogen for DC-based immunotherapy and highlights the potential of a combinatorial regimen with cisplatin for therapeutic intervention.
CANCER CELL INTERNATIONAL
(2021)
Article
Immunology
Jie Lu, Kun Sun, Huiping Yang, Dan Fan, He Huang, Yi Hong, Shuiyan Wu, HuiTing Zhou, Fang Fang, YanHong Li, Lijun Meng, Jie Huang, Zhenjiang Bai
Summary: Sepsis-induced systemic inflammation impairs hematopoietic stem and progenitor cells (HSPCs) in producing dendritic cells (DCs), leading to decreased numbers and functions of DCs. The study found significantly lower levels of pDCs and cDCs in sepsis patients' peripheral blood samples compared to healthy donors, along with increased DC apoptosis and reduced HSPC progenitors. Additionally, sepsis-associated cytokines like G-CSF and IFN-gamma were shown to inhibit DC development from cultured HSPCs.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Shanmuga Sundaram Mahalingam, Sangeetha Jayaraman, Adhvika Arunkumar, Holly M. Dudley, Donald D. Anthony, Carey L. Shive, Jeffrey M. Jacobson, Pushpa Pandiyan
Summary: Individuals aged >61 show intrinsic changes in T cell responses during SARS-CoV-2 infection, including reduced cytokine production capacity and increased PD-1(+) cells. These findings may provide insights into dysregulated immune responses in COVID-19 in the elderly.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Helene M. S. Eld, Peter R. Johnsen, Emilie M. Nielsen, Frederikke Z. Jorgensen, Marie Lindstrom-Svendsen, Mara Baldry, Hanne Ingmer, Hanne Frokiaer
Summary: This study investigated the IL-12 potentiating effect of mannan pre-treatment of bone marrow-derived dendritic cells prior to stimulation with clinical MRSA strains. It was found that mannan almost doubled IL-12 production and increased bacterial uptake and reactive oxygen species (ROS) production.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Dermatology
Shilpi Giri, Heikrujam Thoihen Meitei, Amrita Mishra, Girdhari Lal
Summary: This study investigates the mechanistic role of gamma delta T cells in costimulatory blockade-induced transplantation tolerance. Depletion of gamma delta T cells leads to acute rejection of skin allografts despite tolerogen treatment. Tolerogen treatment promotes the generation of regulatory V gamma 2(+) gamma delta T cells and suppresses IFN-gamma production. Adoptive transfer of these regulatory V gamma 2(+) gamma delta T cells prolongs the survival of allografts.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Clinical Neurology
Hyun-Sik Yang, Can Zhang, Becky C. Carlyle, Sherri Y. Zhen, Bianca A. Trombetta, Aaron P. Schultz, Jeremy J. Pruzin, Colleen D. Fitzpatrick, Wai-Ying W. Yau, Dylan R. Kirn, Dorene M. Rentz, Steven E. Arnold, Keith A. Johnson, Reisa A. Sperling, Jasmeer P. Chhatwal, Rudolph E. Tanzi
Summary: The study found that IL-12p70 and IFN-γ are associated with cognitive decline in cognitively unimpaired elderly, and this association may be related to Aβ levels. This suggests that immune dysregulation plays an important role in early-stage cognitive decline.
ALZHEIMERS & DEMENTIA
(2022)
Article
Immunology
Ella Bhagyaraj, Hongbin Wang, Xinghong Yang, Carol Hoffman, Ali Akgul, Zakia Goodwin, David W. Pascual
Summary: znBAZ infection induces an increase and activation of lung NK cells in mice, which play a crucial role in promoting lung DC activation, migration, and the development of protective CD8(+) T cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Allison N. Gerber, Kaveh Abdi, Nevil J. Singh
Summary: The research identifies a mechanism involving the cytokine IL-12 that can control the spread of pathogens throughout the body, although it does not affect parasite burden at the infection site. The findings suggest that early secretion of IL-12p40 monomers by sentinel cells may prepare distal host tissues for potential pathogen arrival.
Article
Public, Environmental & Occupational Health
Sajjad Ahmad, Jawad Ahmed, Eman H. Khalifa, Farhad Ali Khattak, Anwar Sheed Khan, Syed Umar Farooq, Sannaa M. A. Osman, Magdi M. Salih, Nadeem Ullah, Taj Ali Khan
Summary: This study aimed to characterize the mutations in genes of the IL-12/23/ISG15-IFN-gamma pathway in severe tuberculosis patients. Through the investigation of four clinically suspected tuberculosis patients, mutations were identified in IL-12R beta 1, STAT1, NEMO, and CYBB genes, which are associated with the onset of tuberculosis and immune dysfunction.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2023)
Article
Medicine, General & Internal
Mohamed F. Mandour, Pyone Pyone Soe, Anne-Sophie Castonguay, Jacques Van Snick, Jean-Paul Coutelier
Summary: This study investigates the mechanisms by which the interaction between infectious agent molecules and early innate responses enhances the early inhibition of cancer growth, and specifically evaluates the role of Interleukin-12 (IL-12). The results show that CpG-protected mice against the early growth of plasmacytoma TEPC.1033.C2 cells through the ligation of toll-like receptor 9 (TLR9). This protection is dependent on IL-12 and gamma-interferon, and is confirmed in a model of early mesothelioma AB1 cell growth through the stimulation of TLR3 and TLR7/8.
FRONTIERS IN MEDICINE
(2023)
Article
Medicine, Research & Experimental
Hung Le, Paul Spearman, Stephen N. Waggoner, Karnail Singh
Summary: Accumulation of activated NK cells in tissues during Ebola virus infection contributes to EVD pathogenesis. Immunization with Ebola VLPs provides rapid, NK cell-mediated protection against Ebola challenge. VP40 protein stimulates NK cell functions in an IL-12- and IL-18-dependent manner involving CD14(+) and CD14(-) accessory cells.
Article
Medicine, Research & Experimental
Claudia Augusta Di Trani, Assunta Cirella, Leire Arrizabalaga, Maite Alvarez, Angela Bella, Myriam Fernandez-Sendin, Joan Salvador Russo-Cabrera, Celia Gomar, Nuria Ardaiz, Alvaro Teijeira, Elixabet Bolanos, Jose Gonzalez-Gomariz, Itziar Otano, Fernando Aranda, Belen Palencia, Aina Segues, Shuyu Huang, Sander M. J. van Duijnhoven, Andrea van Elsas, Ignacio Melero, Pedro Berraondo
Summary: This study aims to improve the efficacy of cancer immunotherapy by enhancing the tethering of IL-12 to tumor cells. The fusion of IL-12 with diabodies successfully achieved binding to tumor cells and reduced its systemic availability. This strategy could potentially enhance the therapeutic effects and minimize the toxicity of IL-12 in cancer treatment.
MOLECULAR THERAPY NUCLEIC ACIDS
(2023)
Article
Multidisciplinary Sciences
Ousheng Liu, Junji Xu, Fu Wang, Wenwen Jin, Peter Zanvit, Dandan Wang, Nathan Goldberg, Alexander Cain, Nancy Guo, Yichen Han, Andrew Bynum, Guowu Ma, Songlin Wang, Zhangui Tang, Wanjun Chen
Summary: Research has shown that ADSCs can produce IL-33 in response to stimulation, which alleviates autoimmune diseases by promoting the proliferation of regulatory T cells (Tregs). This previously unrecognized immunoregulatory function of ADSCs through IL-33 production may have potential clinical applications.
Article
Cell Biology
Helena Car, Gonca E. Karahan, Geertje J. Dreyer, Simone H. Brand-Schaaf, Aiko P. J. de Vries, Cees van Kooten, Cynthia S. M. Kramer, Dave L. Roelen, Frans H. J. Claas, Sebastiaan Heidt
Summary: The study modified the IgG HLA antibody detection method and developed a new method for screening IgA HLA antibodies. Results of the study indicate a low incidence of IgA HLA antibodies in individuals exposed to alloantigens.
Article
Surgery
Marlies E. J. Reinders, Koen E. Groeneweg, Sanne H. Hendriks, Jonna R. Bank, Geertje J. Dreyer, Aiko P. J. de Vries, Melissa van Pel, Helene Roelofs, Volkert A. L. Huurman, Paula Meij, Dirk J. A. R. Moes, Willem E. Fibbe, Frans H. J. Claas, Dave L. Roelen, Cees van Kooten, Jesper Kers, Sebastiaan Heidt, Ton J. Rabelink, Johan W. de Fijter
Summary: Early tacrolimus withdrawal with MSC therapy after renal transplantation is safe and feasible, without increased rejection and with preserved renal function. MSC therapy is a potentially useful approach in this context.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Review
Immunology
Jacob J. E. Koopman, Mieke F. van Essen, Helmut G. Rennke, Aiko P. J. de Vries, Cees van Kooten
Summary: The membrane attack complex C5b-9, a key factor in the complement pathways, is implicated in various kidney diseases by causing cellular injury and inflammation. Understanding the deposition of C5b-9 in the kidney is crucial for predicting treatment outcomes and developing novel therapies targeting its formation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Chiara Divella, Alessandra Stasi, Rossana Franzin, Michele Rossini, Paola Pontrelli, Fabio Sallustio, Giuseppe Stefano Netti, Elena Ranieri, Luca Lacitignola, Francesco Staffieri, Alberto Maria Crovace, Giuseppe Lucarelli, Pasquale Ditonno, Michele Battaglia, Mohamed R. Daha, Peter van der Pol, Cees van Kooten, Giuseppe Grandaliano, Loreto Gesualdo, Giovanni Stallone, Giuseppe Castellano
Summary: Pentraxin family member PTX3 plays a crucial role in I/R injury and may be a potential therapeutic target for preventing complement-induced renal I/R injury.
Article
Immunology
Koen E. Groeneweg, Frederique A. van der Toorn, Dave L. Roelen, Cees van Kooten, Sebastiaan Heidt, Frans H. J. Claas, Marlies E. J. Reinders, Johan W. de Fijter, Darius Soonawala
Summary: This study found that female recipients of spousal donor kidney transplants are more likely to have donor-specific pre-immunization, which increases the risk of acute antibody-mediated rejection. Early ABMR can lead to inferior graft survival and is more common in women receiving kidney from spouse, highlighting the importance of including SAB assay in the pre-transplant assessment for this group.
TRANSPLANT IMMUNOLOGY
(2021)
Meeting Abstract
Rheumatology
E. J. Arends, M. Zlei, C. M. Tipton, Z. Osmani, S. Kamerling, T. Rabelink, I. Sanz, J. J. M. Van Dongen, C. Van Kooten, Y. K. O. Teng
ANNALS OF THE RHEUMATIC DISEASES
(2021)
Review
Pharmacology & Pharmacy
Carla M. A. van Alem, Josbert M. Metselaar, Cees van Kooten, Joris I. Rotmans
Summary: Liposomes are considered ideal carriers for anti-inflammatory drugs due to their ability to target sites of inflammation and increase local efficacy. However, clinical translation of liposomal nanomedicines in the field of inflammatory diseases still poses challenges. Recent research has focused on innovations and cutting-edge developments in liposomal treatment.
Article
Biochemistry & Molecular Biology
Cleo C. L. van Aanhold, Angela Koudijs, Kyra L. Dijkstra, Ron Wolterbeek, Jan A. Bruijn, Cees van Kooten, Hans J. Baelde
Summary: The study found that transfection with sFLT1 did not aggravate renal fibrosis and peritubular capillary loss, but reduced the expression of pro-fibrotic and pro-inflammatory markers. sFLT1 mRNA levels were not correlated with interstitial fibrosis or PTC loss in human samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Florine Seidel, Robert Kleemann, Wim van Duyvenvoorde, Nikki van Trigt, Nanda Keijzer, Sandra van der Kooij, Cees van Kooten, Lars Verschuren, Aswin Menke, Amanda J. Kiliaan, Johnathan Winter, Timothy R. Hughes, B. Paul Morgan, Frank Baas, Kees Fluiter, Martine C. Morrison
Summary: Complement inhibition with an anti-C5 antibody is not effective in reducing the progression of NASH but is beneficial in established atherosclerosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Education, Scientific Disciplines
Suzanne Fikrat-Wevers, Karen Stegers-Jager, Marleen Groenier, Andries Koster, Jan Hindrik Ravesloot, Renske Van Gestel, Anouk Wouters, Walter van den Broek, Andrea Woltman
Summary: Applicant perceptions of selection methods can affect motivation, performance and withdrawal, and this study aimed to examine how different applicant subgroups perceive various selection methods. The findings showed that applicants prefer selection methods that give them a sense of control and allow them to showcase attributes beyond cognitive abilities. However, conflicting preferences and concerns were also identified.
Article
Education & Educational Research
S. Fikrat-Wevers, K. M. Stegers-Jager, P. M. Afonso, A. S. Koster, R. A. Van Gestel, M. Groenier, J. H. Ravesloot, A. Wouters, W. W. Van Den Broek, A. M. Woltman
Summary: This study examined how selection procedures in health professions education impact student diversity. The results showed that different selection tools have different effects on the chances of selection for subgroups of applicants. The study highlights the importance of continuous evaluation and the use of alternative tools in order to improve diversity in health professions education.
ADVANCES IN HEALTH SCIENCES EDUCATION
(2023)
Article
Multidisciplinary Sciences
Vera M. A. Broks, Karen M. Stegers-Jager, Jeroen van der Waal, Walter W. van den Broek, Andrea M. Woltman
Summary: This study aimed to assess the impact of the COVID-19 outbreak on perceived stress levels of medical students and explore the relationship between social support and stress during the pandemic. The results showed that medical students experienced higher levels of perceived stress during the outbreak, especially those with lower levels of social support. Therefore, medical schools should optimize social support to minimize crisis-induced stress.
Article
Education & Educational Research
S. Fikrat-Wevers, W. E. De Leng, W. W. van den Broek, A. M. Woltman, K. M. Stegers-Jager
Summary: This study evaluated the relationship between four free preparatory activities and the demographic composition of participating and non-participating applicants. The results showed that these activities contribute to student diversity in medical education, but participation is not directly associated with early academic performance.
BMC MEDICAL EDUCATION
(2023)
Article
Gastroenterology & Hepatology
Monique T. A. de Beijer, Karel Bezstarosti, Robbie Luijten, Wouter A. S. Doff, Patrick P. C. Boor, Roel F. A. Pieterman, Rachid Bouzid, Paula J. Biesta, Jan N. M. Ijzermans, Michail Doukas, Robert A. de Man, Andrea M. Woltman, Jeroen A. A. Demmers, Sonja I. Buschow
Summary: In this study, the HLA-I immunopeptidome of isolated primary human hepatocytes was characterized, revealing HBV-derived and tumor-associated antigen-derived peptides. These findings contribute to the development of antigen-specific immunotherapy for chronic HBV infection and hepatocellular carcinoma.
Article
Medical Laboratory Technology
Tirsa T. van Duijl, L. Renee Ruhaak, Cees van Kooten, Johan W. de Fijter, Christa M. Cobbaert
Summary: This study explored the potential of LC-MS/MS as a next-generation technology for multiplex urinary protein measurement. It overcomes nonselectivity and lack of standardization by directly measuring well-defined molecular proteins.
JOURNAL OF APPLIED LABORATORY MEDICINE
(2022)
