Article
Biochemical Research Methods
Elvis Duran-Sierra, Shuna Cheng, Rodrigo Cuenca, Beena Ahmed, Jim Ji, Vladislav V. Yakovlev, Mathias Martinez, Moustafa Al-Khalil, Hussain Al-Enazi, Javier A. Jo
Summary: Early detection is crucial for improving the survival rate and quality of life for oral cancer patients. However, it is often challenging to distinguish dysplastic and early-stage cancerous oral lesions from benign lesions during standard clinical examination. This study aimed to identify novel biochemical and metabolic autofluorescence biomarkers of oral dysplasia and cancer using multispectral autofluorescence lifetime imaging (maFLIM) endoscopy technologies. Clinical images of benign, precancerous, and cancerous lesions were acquired using a novel maFLIM endoscope, and statistical analyses were performed to identify maFLIM features that can differentiate dysplastic/cancerous from benign oral lesions. Fourteen spectral and time-resolved maFLIM features were found to provide contrast between dysplastic/cancerous and benign oral lesions, representing potential biomarkers of oral dysplasia and cancer. This is the first demonstration of clinical widefield maFLIM endoscopic imaging of biochemical and metabolic autofluorescence biomarkers for early detection of oral cancer.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Biochemistry & Molecular Biology
Allison Podsednik, Jinxia Jiang, Annemarie Jacob, Lin Z. Li, He N. Xu
Summary: The study evaluated the therapeutic responses of triple-negative breast cancer cells under different drug treatments using optical redox imaging, showing the effects of different drugs on cells and their synergistic interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
He N. Xu, Sarah Gourmaud, Allison Podsednik, Xiaofan Li, Huaqing Zhao, Frances E. Jensen, Delia M. Talos, Lin Z. Li
Summary: The translation above discusses a study on the decline in NAD levels in brain tissue of Alzheimer's disease models and the use of optical redox imaging to explore new biomarkers for AD. The study found that NADH, Fp, and the redox ratio were associated with AD, and suggested that ORI could be used to conveniently study the metabolism of freshly dissected brain tissues.
Article
Biochemistry & Molecular Biology
Ekaterina O. Bryanskaya, Andrey Y. Vinokurov, Angelina I. Dolgikh, Andrey Dunaev, Plamena R. Angelova, Andrey Y. Abramov
Summary: FAD autofluorescence in cells can assess enzymatic activity, and its intensity variations may be related to different cell types and tissues. High levels of FAD autofluorescence can indicate cell pathology and potentially predict the occurrence of apoptosis and necrosis.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Moritz A. Niederschweiberer, Patrick M. Schaefer, Larry N. Singh, Ludwig Lausser, Devyani Bhosale, Raphael Hesse, Enrico Calzia, Hans A. Kestler, Angelika Rueck, Douglas C. Wallace, Bjoern von Einem, Christine A. F. von Arnim
Summary: Alzheimer's disease affects over 30 million people globally and mitochondrial dysfunction plays a significant role in its development. However, the selective vulnerability and associated mitochondrial dysfunction remain unresolved.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Reproductive Biology
Tiffany C. Y. Tan, Hannah M. Brown, Jeremy G. Thompson, Sanam Mustafa, Kylie R. Dunning
Summary: This study investigated the use of optical imaging to measure the metabolism in the cumulus and oocyte compartments of the cumulus oocyte complex (COC). The results showed that the optical redox ratio accurately reflected metabolism in the oocyte compartment and could detect metabolic differences associated with poor oocyte quality. This label-free optical imaging technique has the potential to assess oocyte developmental competence.
BIOLOGY OF REPRODUCTION
(2022)
Article
Engineering, Multidisciplinary
Joshua M. Herzog, Volker Sick
Summary: Fluorescence imaging is a useful tool for characterizing tissue metabolic state and discriminating between microbial species. However, current techniques are often expensive and require specialized training. This study proposes a low-cost and robust imaging technique using ultraviolet-induced fluorescence of pyridine nucleotides and flavins in microbial samples. The technique shows high sensitivity and precision in distinguishing microbial species, making it a feasible option for rapid and low-cost microbial sample characterization.
MEASUREMENT SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Fariha Ansari, Belem Yoval-Sanchez, Zoya Niatsetskaya, Sergey Sosunov, Anna Stepanova, Christian Garcia, Edward Owusu-Ansah, Vadim Ten, Ilka Wittig, Alexander Galkin
Summary: Accurate measurement of the content of mitochondrial complex I and alpha-ketoglutarate dehydrogenase complex is crucial for disease research and evaluation of treatment efficacy.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Physics, Multidisciplinary
Yifan Qin, Yuanqin Xia
Summary: Two-photon fluorescence microscopy can provide physiological and pathological information from biological tissues by observing intrinsic fluorophores such as NADH and FAD. A new imaging system demonstrated in this paper allows for simultaneous capture of autofluorescence images from NADH and FAD with high excitation efficiency and minimal spectral bleed-through.
FRONTIERS IN PHYSICS
(2021)
Article
Biology
Veronika Miskolci, Kelsey E. Tweed, Michael R. Lasarev, Emily C. Britt, Alex J. Walsh, Landon J. Zimmerman, Courtney E. McDougal, Mark R. Cronan, Jing Fan, John-Demian Sauer, Melissa C. Skala, Anna Huttenlocher
Summary: This study used two-photon imaging of autofluorescent metabolic coenzymes NAD(P)H and FAD to assess macrophage metabolism in the wound microenvironment. Results showed that inhibiting glycolysis led to a more oxidized intracellular redox state in macrophages, and infection and thermal injury induced a more oxidized redox state in wounded tissues. Kinetic analysis revealed a shift toward a more reduced redox state during tissue repair. Metformin improved tissue repair by reducing TNF alpha+ wound macrophages and making the intracellular redox state more reduced, while depletion of STAT6 impaired regeneration by increasing TNF alpha+ wound macrophages and making the redox state more oxidized. The study suggests that autofluorescence of NAD(P)H and FAD can be used to probe the temporal and spatial regulation of macrophage metabolism during tissue damage and repair.
Article
Biology
Xingbo Yang, Gloria Ha, Daniel J. Needleman
Summary: A new method was developed to infer mitochondrial metabolic fluxes in living cells from fluorescence lifetime imaging of NADH, revealing a homeostasis of ETC flux in mouse oocytes and a subcellular spatial gradient of ETC flux.
Article
Biochemistry & Molecular Biology
Sviatlana Kalinina, Christian Freymueller, Nilanjon Naskar, Bjoern von Einem, Kirsten Reess, Ronald Sroka, Angelika Rueck
Summary: Metabolic FLIM is used to image bioenergetic status in cells and tissue based on fluorescence lifetime of coenzymes. A new algorithm utilizing NAD(P)H and FAD lifetimes to calculate FLIRR has been developed. The study extended FLIRR approach and introduced FLIRR1, FLIRR2, and FLIRR3 to analyze the redox state of cells with different enzymes. The significance of extended FLIRR was compared to the metabolic index, showing the highest difference for FLIRR1 in tumor and normal cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
A. M. El-naggar, Mohamed Bakr Mohamed, M. M. Osman, Zein K. Heiba, A. M. Kamal, A. M. Aldhafiri, Latifah F. Alotaibi
Summary: In this study, perovskite solar cell films were prepared using different molarity concentrations of CH3NH3I and PbI2, with and without the use of chlorobenzene as an anti-solvent. The effects of these factors on the film properties were examined through X-ray diffraction analysis, scanning electron microscopy, and optical studies. It was found that the concentration of perovskite precursors and the use of anti-solvent play a role in determining the film characteristics.
RESULTS IN PHYSICS
(2021)
Article
Pharmacology & Pharmacy
Peter Tsvetkov, Julia Adler, Romano Strobelt, Yaarit Adamovich, Gad Asher, Nina Reuven, Yosef Shaul
Summary: This passage demonstrates the interaction between SIRT1 and NQO1, indicating that SIRT1 can regulate the expression of NQO1 and exert a protective role under mitochondrial stress.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemical Research Methods
Amani A. Gillette, Rebecca A. DeStefanis, Stephanie L. Pritzl, Dustin A. Deming, Melissa C. Skala
Summary: This study investigates the effects of Bcl-2 family protein inhibitors on cellular metabolism and finds that the optical redox ratio (ORR) is sensitive to changes in mitochondrial function and cell senescence, which can occur independently of cell viability. The metabolic changes caused by ABT-263 treatment can be mitigated by combination with mTORC1/2 inhibitors.
JOURNAL OF BIOMEDICAL OPTICS
(2022)