Article
Andrology
Junaid Kashir, Bhavesh Mistry, Maha Adel Gumssani, Muhammad Rajab, Reema Abu-Dawas, Falah AlMohanna, Michail Nomikos, Celine Jones, Raed Abu-Dawud, Nadya Al-Yacoub, Kevin Coward, F. Anthony Lai, Abdullah M. Assiri
Summary: The study shows that with advancing male age, levels and variability of PLC zeta protein and mRNA in sperm decrease. There are also changes in the localization patterns of PLC zeta in sperm from older male mice.
ASIAN JOURNAL OF ANDROLOGY
(2021)
Article
Biology
Naoki Hirose, Yasuyuki Kikuchi, Atsuko Kageyama, Hibiki Sugita, Miu Sakurai, Yui Kawata, Jumpei Terakawa, Teruhiko Wakayama, Junya Ito, Naomi Kashiwazaki
Summary: During mammalian fertilization, intracellular calcium oscillations are needed for oocyte activation. Sperm lacking PLC zeta can still induce calcium rises in oocytes after IVF but not ICSI. Additional activation treatments such as SrCl2 and PLC zeta mRNA can rescue the developmental ability of Plcz1(-/-) sperm-derived oocytes after ICSI, leading to successful term development and healthy offspring.
Article
Biochemistry & Molecular Biology
Xiaopei Zhou, Zhenxing Liu, Weimin Jia, Meiqi Hou, Xianqin Zhang
Summary: This study investigates the mechanisms underlying the effects of Actl7a gene knockout on sperm acrosome formation, male infertility, and sperm-zona pellucida binding ability during fertilization. The findings suggest that Actl7a deficiency leads to malformed sperm acrosomes, male infertility, and impaired sperm-ZP binding ability. Furthermore, abnormal localization and expression of the PLCZ1 protein in misshapen sperm are identified, resulting in reduced calcium oscillations in oocytes.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Chemistry, Medicinal
Haia M. R. Abdulsamad, Zoha F. F. Murtaza, Hessa M. M. AlMuhairi, Wjdan S. S. Bafleh, Salma A. A. AlMansoori, Shaikha A. A. AlQubaisi, Hamdan Hamdan, Junaid Kashir
Summary: Oocyte activation, driven by calcium oscillations mainly regulated by PLC zeta, is crucial for mammalian fertilization and embryogenesis. Failure of calcium release or defects in related mechanisms can lead to infertility in humans. Exploration of PLC zeta and its patterns in human sperm may offer potential for diagnosing and treating human fertility issues. This review provides a comprehensive update on the clinical associations between calcium release, PLC zeta, oocyte activation, and human fertility, discussing their implications in defective embryogenesis and infertility treatments.
Article
Cell Biology
Haixia Chen, Peng Li, Xiaoling Du, Yiding Zhao, Lingling Wang, Ye Tian, Xueru Song, Ling Shuai, Xiaohong Bai, Lingyi Chen
Summary: Mutation in the SEPTIN12 gene is associated with male infertility. Male mice with Septin12 knockout are infertile and have abnormal sperm, but fertility can be restored with artificial oocyte activation. Loss of PLC zeta might be the reason for failed fertilization in Septin12-deficient sperm.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Medicinal
Junaid Kashir, Bhavesh V. V. Mistry, Lujain BuSaleh, Michail Nomikos, Sarah Almuqayyil, Raed Abu-Dawud, Nadya AlYacoub, Hamdan Hamdan, Saad AlHassan, F. Anthony Lai, Abdullah M. M. Assiri, Serdar Coskun
Summary: The application of antigen unmasking/retrieval (AUM) enhances the visualization of PLC zeta in human sperm, allowing for accurate assessment of sperm fertilization capacity.
Review
Genetics & Heredity
Veena Unnikrishnan, John Kastelic, Jacob Thundathil
Summary: In cattle, the efficiency of ICSI is hindered by failed oocyte activation post sperm injection. Research focusing on proteins like Na/K-ATPase and PLC zeta is key to improving the success rate. Enhancing the efficiency of bovine ICSI has significant implications for the cattle breeding industry.
Article
Genetics & Heredity
Jing Dai, Tianlei Zhang, Jing Guo, Qinwei Zhou, Yifan Gu, Jue Zhang, Liang Hu, Yurong Zong, Juan Song, Shuoping Zhang, Can Dai, Fei Gong, Guangxiu Lu, Wei Zheng, Ge Lin
Summary: Genetic variations in the ACTL9 gene in male individuals have been found to cause total fertilization failure (TFF). These variations disrupt the perinuclear theca structure and affect the localization of PLCz in sperm, leading to abnormal calcium oscillations in oocytes and subsequent TFF. Overcoming TFF can be achieved through assisted oocyte activation with calcium ionophore exposure.
AMERICAN JOURNAL OF HUMAN GENETICS
(2021)
Review
Cell Biology
Katherine A. Maniates, Andrew Singson
Summary: Complementary forward and reverse genetic approaches have led to a recent surge in the discovery of fertilization genes in various model systems. The number of genetically validated gamete surface molecules has more than doubled in the past few years, with all sperm fertilization genes encoding transmembrane or secreted molecules. However, the discovery of genes encoding oocyte molecules has lagged behind. This review discusses potential experimental biases and biological reasons that may have hindered the identification of egg fertilization genes, as well as current strategies to overcome these obstacles.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Developmental Biology
Yuhkoh Satouh
Summary: This article reports on the study of oocyte activation induced by PLCZ1 in mice, as well as the generation of PLCZ1-deficient mouse models using genome-editing techniques. The findings show that PLCZ1 deficiency in mice leads to subfertility but can be fully restored by intracytoplasmic sperm injection, indicating a PLCZ1-independent oocyte activation mechanism. These findings raise new questions in the field of sperm biology and have implications for the treatment and phenotypic interpretation of oocyte activation failure in patients.
Article
Multidisciplinary Sciences
Yoon Hee Jung, Hsiao-Lin Wang, Daniel Ruiz, Brianna J. Bixler, Hannah Linsenbaum, Jian-Feng Xiang, Samantha Forestier, Andrew M. Shafik, Peng Jin, Victor G. Corces
Summary: This study demonstrates that exposure of pregnant mice to bisphenol A (BPA) can lead to transgenerational transmission of obesity in F2 progeny up to the F6 generation. Chromatin accessibility analysis reveals alterations near binding motifs for CCCTC-binding factor (CTCF) at two cis-regulatory elements of the Fto gene, which correlate with the transmission of obesity. The interaction between these cis-regulatory elements and the Irx3 and Irx5 genes, involved in the differentiation of appetite-controlling neurons, is increased in sperm of obese mice. Deletion of the CTCF site in Fto results in mice that have normal food intake and fail to become obese when ancestrally exposed to BPA.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Mohammad Ishraq Zafar, Shi Lu, Honggang Li
Summary: The fertilizing spermatozoon is a highly specialized cell that plays a crucial role in fertilization and embryo development. PLC zeta and PAWP are main candidates as oocyte activated factors during fertilization, but their mechanisms and treatment methods still need further research and exploration.
CELL AND BIOSCIENCE
(2021)
Article
Obstetrics & Gynecology
Jing Wang, Jintao Zhang, Xueping Sun, Yuting Lin, Lingbo Cai, Yugui Cui, Jiayin Liu, Mingxi Liu, Xiaoyu Yang
Summary: The study identified novel compound heterozygous variants in ACTL7A as a genetic factor for human TFF and successfully rescued TFF through AOA.
HUMAN REPRODUCTION
(2021)
Review
Obstetrics & Gynecology
Junaid Kashir, Durga Ganesh, Celine Jones, Kevin Coward
Summary: Oocyte activation deficiency (OAD) is a major cause of ICSI cycle failure. Assisted oocyte activation (AOA) is a method that artificially increases the levels of intracytoplasmic calcium ions (Ca2+) through mechanical, electrical, and/or chemical stimuli. However, the safety and efficacy of AOA are still controversial. More research is needed to determine the safety and efficacy of AOA techniques.
HUMAN REPRODUCTION OPEN
(2022)
Review
Developmental Biology
Nicoletta Tarozzi, Marco Nadalini, Giovanni Coticchio, Carlotta Zaca, Cristina Lagalla, Andrea Borini
Summary: Sperm is crucial for reconstitution of embryonic diploidy and specialized developmental functions. It triggers activation through PLC-zeta after gamete fusion, impacting fertilization and chromosome segregation. Sperm-specific epigenetic factors also contribute to embryonic development and have implications for assisted reproduction treatments.
MOLECULAR HUMAN REPRODUCTION
(2021)
Review
Neurosciences
Bared Safieh-Garabedian, Michail Nomikos, Nayef Saade
NEUROSCIENCE LETTERS
(2019)
Article
Multidisciplinary Sciences
Sahar I. Da'as, Angelos Thanassoulas, Brian L. Calver, Konrad Beck, Rola Salem, Alaaeldin Saleh, Iris Kontogianni, Ali Al-Maraghi, Gheyath K. Nasrallah, Bared Safieh-Garabedian, Egon Toft, George Nounesis, F. Anthony Lai, Michail Nomikos
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2019)
Article
Cell Biology
Josephine Bradley, Iestyn Pope, Yisu Wang, Wolfgang Langbein, Paola Borri, Karl Swann
JOURNAL OF CELL SCIENCE
(2019)
Article
Cardiac & Cardiovascular Systems
Monika Seidel, Camille Rabesahala de Meritens, Louisa Johnson, Dimitris Parthimos, Mark Bannister, Nia Lowri Thomas, Esizaze Ozekhome-Mike, Francis Anthony Lai, Spyros Zissimopoulos
Summary: The study characterizes the network of interactions regulating the activity of the RyR2 homotetramer, identifying the importance of a peptide sequence bridging the beta 8 with beta 9 strand in mediating N-terminus self-association. The beta 8-beta 9 loop is crucial for N-terminal intersubunit interaction, while also interacting with the C-terminal channel pore region in a Ca2+-independent manner. Deletion of the beta 8-beta 9 sequence affects RyR2 subunit oligomerization and intracellular Ca2+ mobilization, indicating a role in channel opening.
CARDIOVASCULAR RESEARCH
(2021)
Review
Cell Biology
Alaaeldin Saleh, Junaid Kashir, Angelos Thanassoulas, Bared Safieh-Garabedian, F. Anthony Lai, Michail Nomikos
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Editorial Material
Developmental Biology
Karl Swann
Article
Developmental Biology
Anna Storey, Khalil Elgmati, Yisu Wang, Paul Knaggs, Karl Swann
Summary: The study revealed that mouse eggs are about 10 times more sensitive to InsP(3) than human eggs, and have consistently higher ATP levels. As a result, mouse eggs can undergo Ca2+ oscillations in response to Sr2+ stimulation, while human eggs may be unresponsive due to lower cytosolic ATP levels.
MOLECULAR HUMAN REPRODUCTION
(2021)
Article
Cell Biology
Andreia de Almeida, Dimitris Parthimos, Holly Dew, Oliver Smart, Marie Wiltshire, Rachel J. Errington
Summary: Cells require aquaporins for rapid adaptation to changes in volume and osmotic pressure, as well as metabolic stimuli. The differential expression of AQP3 in prostate cancer cell panels suggests its importance in cellular adaptation to hypoxic conditions. Machine learning classification based on morphological and texture features can accurately distinguish between different cell lines and their exposure to hypoxia.
Article
Developmental Biology
Yisu Wang, Iestyn Pope, Henry Brennan-Craddock, Emma Poole, Wolfgang Langbein, Paola Borri, Karl Swann
Summary: The primary effect of saturated fatty acid PA on mouse oocytes is to disrupt the structure of the ER, without affecting the mitochondria or Ca2+ stores.
Article
Biology
Chris F. Graham, Shane Windsor, Anna Ajduk, Thanh Trinh, Anna Vincent, Celine Jones, Kevin Coward, Dilraj Kalsi, Magdalena Zernicka-Goetz, Karl Swann, Adrian L. R. Thomas
Summary: Morphokinetics of mouse zygotes were studied at different stages, revealing changing patterns of shape and area, as well as the development of vortices during cell division. These shape and area cycles may play a role in promoting exchange with the environment, while the persistent circulation in the cytoplasm could aid in communication between the cell's interior and periphery.
Article
Biochemistry & Molecular Biology
Sahar Isa Da'as, Waseem Hasan, Rola Salem, Nadine Younes, Doua Abdelrahman, Iman A. Mohamed, Arwa Aldaalis, Ramzi Temanni, Lisa Sara Mathew, Stephan Lorenz, Magdi Yacoub, Michail Nomikos, Gheyath K. Nasrallah, Khalid A. Fakhro
Summary: The study investigated the cardiac impairment related to HCM by generating a zebrafish mypbc3-knockout model. The knockout zebrafish exhibited significant heart morphological alterations and cardiomyocyte hyperplasia in larval stages with preserved cardiac contractility. However, adult zebrafish showed thickened ventricular walls, reduced heart function, and decreased swimming abilities, indicating the crucial role of cMyBP-C variants in the initiation and progression of HCM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Proceedings Paper
Optics
Paola Borri, Josephine Bradley, Iestyn Pope, Wolfgang Langbein, Karl Swann
LABEL-FREE BIOMEDICAL IMAGING AND SENSING (LBIS) 2019
(2019)
Review
Developmental Biology
Josephine Bradley, Karl Swann
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
(2019)
Proceedings Paper
Engineering, Electrical & Electronic
I. Pope, F. Masia, J. Bradley, K. Ewan, A. Karuna, L. Payne, I. Guschina, J. Harwood, K. Swann, T. Dale, P. Watson, W. Langbein, P. Borri
2018 20TH ANNIVERSARY INTERNATIONAL CONFERENCE ON TRANSPARENT OPTICAL NETWORKS (ICTON)
(2018)
Article
Cell Biology
Jessica R. Sanders, Bethany Ashley, Anna Moon, Thomas E. Woolley, Karl Swann
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2018)
