Article
Physiology
Francesca Soglia, Martina Bordini, Maurizio Mazzoni, Martina Zappaterra, Mattia Di Nunzio, Paolo Clavenzani, Roberta Davoli, Adele Meluzzi, Federico Sirri, Massimiliano Petracci
Summary: This study aimed to investigate the evolution of VIM and DES proteins and genes in the Pectoralis major muscles of fast-growing and medium-growing meat-type chickens. The findings suggest a relationship between muscle regeneration and the growth rate of the chickens, and support the use of VIM and DES as molecular markers for these cellular processes.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Chen Ni, Xiaohan Lou, Xiaohan Yao, Linlin Wang, Jiajia Wan, Xixi Duan, Jialu Liang, Kaili Zhang, Yuanyuan Yang, Li Zhang, Chanjun Sun, Zhenzhen Li, Ming Wang, Linyu Zhu, Dekang Lv, Zhihai Qin
Summary: This study reveals a mechanism by which a subset of zinc-transporter positive cancer-associated fibroblasts directly interacts with lung cancer cells through gap junctions, promoting chemoresistance in tumors. This finding provides insights into the role of fibroblast-tumor cell interactions in lung cancer chemo-resistance.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Pablo Hernandez-Ortego, Remedios Torres-Montero, Elvira de la Pena, Felix Viana, Jorge Fernandez-Trillo
Summary: TRPM8 is a non-selective cation channel widely expressed in primary sensory neurons and other tissues. This study characterized the performance of six commercial TRPM8 antibodies in three immunodetection techniques and found that two antibodies were successful in detecting TRPM8 in all approaches, while the other four were only acceptable for specific techniques.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Nurunnajah Lazim, Marjanu Hikmah Elias, Zulazmi Sutaji, Abdul Kadir Abdul Karim, Mohammad Azrai Abu, Azizah Ugusman, Saiful Effendi Syafruddin, Mohd Helmy Mokhtar, Mohd Faizal Ahmad
Summary: This review examines the expression differences of the HOXA10 gene in women with endometriosis compared to those without, across different countries, and discusses its impact on women's fertility. The majority of studies found downregulation of the HOXA10 gene expression, although one study showed evidence of both downregulation and upregulation depending on the localization of endometriotic lesions. Clinically, measuring the expression of the HOXA10 gene is crucial in predicting endometriosis, endometrial receptivity, and the development of pinopodes during the luteal phase.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Anne-Kathrin Lutz, Helen Friedericke Bauer, Valentin Ioannidis, Michael Schoen, Tobias M. Boeckers
Summary: This study validated a set of commercial and homemade SHANK3 antibodies in Western Blotting, synaptic immunocytochemistry, and immunohistochemistry, and found that only a small subset of the antibodies met the criteria of quality and specificity. The study highlights the importance of antibody specificity testing in the research field.
FRONTIERS IN SYNAPTIC NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
E. Anne Martin, Jennifer Carlisle Michel, Jane S. Kissinger, Fabio A. Echeverry, Ya-Ping Lin, John O'Brien, Alberto E. Pereda, Adam C. Miller
Summary: The subcellular positioning and molecular compositions of synapses are crucial for neural circuits. This study investigates the localization of the autism- and epilepsy-associated gene Neurobeachin, the Connexins, and the electrical synapse scaffold ZO1. It is found that Neurobeachin localizes to the electrical synapse independently, and its presence is necessary for the localization of ZO1 and Connexins. This research provides new insights into the molecular complexity and mechanisms of electrical synapse formation.
Article
Genetics & Heredity
Irene Sargiannidou, Violetta Christophidou-Anastasiadou, Andreas Hadjisavvas, George A. Tanteles, Kleopas A. Kleopa
Summary: Oculodentodigital dysplasia syndrome is linked to pathogenic variants in GJA1 gene. A new in-frame deletion (p.Lys134del) was identified in a patient with typical dysmorphic features. Functional study showed reduction in gap junction plaques in cells, with many retained inside.
FRONTIERS IN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Mauricio Serrano-Rubi, Lidia Jimenez, Jacqueline Martinez-Rendon, Marcelino Cereijido, Arturo Ponce
Summary: Gap junctional intercellular communication is reduced in cancer cells compared to normal cells, but the plant-derived substance ouabain enhances this communication in various cancer cell lines through signaling mechanisms involving Csrc, ErK1/2, and ROCK-Rho. This finding suggests a potential therapeutic strategy for influencing cancer progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Clinical Neurology
Riccardo Serra, J. Marc Simard
Summary: This article reviews 45 research articles on intercellular junctions in the ependyma, indicating the important role of ependymal lining ultrastructure in fluid flow interactions in the brain. These findings have implications for understanding and treating CSF-related disorders.
FRONTIERS IN NEUROLOGY
(2023)
Article
Pharmacology & Pharmacy
Catherine I. Soderstrom, Jennifer Larsen, Carolina Owen, David Gifondorwa, David Beidler, Florence H. Yong, Patricia Conrad, Hendrik Neubert, Steven A. Moore, Mohamed Hassanein
Summary: Duchenne muscular dystrophy (DMD) is a degenerative muscular disease with no cure. New advances in gene therapy and understanding of the disease have opened up new treatment opportunities. This article describes a novel Western blot method for monitoring dystrophin expression and assessing treatment efficacy.
Article
Biochemistry & Molecular Biology
Jennifer Ortiz, Yuriy Bobkov, Melissa B. DeBiasse, Dorothy G. Mitchell, Allison Edgar, Mark Q. Martindale, Anthony G. Moss, Leslie S. Babonis, Joseph F. Ryan
Summary: This article investigates the innexin gene in four species of ctenophores and finds that these genes have evolved independently from those of other animals and were established early in the evolution of ctenophores. The study also shows that ctenophores have functional innexin hemichannels that are closely related to those of other animals. Overall, the results suggest that the last common ancestor of animals had channels capable of forming both gap junctions and innexin hemichannels, and that innexin genes have independently evolved in major lineages throughout Metazoa.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Article
Endocrinology & Metabolism
Fereshteh Esfandiari, Fereshteh Chitsazian, Masoumeh Golestan Jahromi, Raha Favaedi, Masood Bazrgar, Reza Aflatoonian, Parvaneh Afsharian, Abbas Aflatoonian, Maryam Shahhoseini
Summary: Endometriosis is a major gynecological disease with unknown cause and no definite cure. Research shows that HOX genes play critical roles in pathophysiology of endometriosis and may assist with disease diagnosis and treatment.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Plant Sciences
Chunmei He, Xuncheng Liu, Jaime A. Teixeira da Silva, Haobin Wang, Tao Peng, Mingze Zhang, Can Si, Zhenming Yu, Jianwen Tan, Jianxia Zhang, Jianping Luo, Jun Duan
Summary: The study identified LEA genes in two orchids, showing strong expression of LEA_1 group genes in seeds and absence in other organs. LEA_1 and LEA_4 group genes in D. officinale were abundant in the protocorm-like body stage and significantly up-regulated in response to abscisic acid and salinity stress, providing insights into plant regeneration.
JOURNAL OF PLANT PHYSIOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Milad Akhlaghi, Mohammad Taebpour, Narges Nikoonahad Lotfabadi, Seyed Marteza Naghib, Neda Jalili, Leila Farahmand, Bibi Fatemeh Haghiralsadat, Mehdi Rahmanian, Davood Tofighi
Summary: Niosomes containing different extracts were synthesized and their physicochemical properties, antibacterial potential, and antitumor effects were evaluated. The niosomes containing Hedera Helix extract showed stronger toxicity and antibacterial potential, as well as a greater impact on the expression of oncogenes than the niosomes containing Glycyrrhiza glabra extract.
NANOTECHNOLOGY REVIEWS
(2022)
Article
Clinical Neurology
Isabel Goncalves, Loureen Oduor, Frank Matthes, Narjess Rakem, Jakob Meryn, Nikolaos-Taxiarchis Skenteris, Anders Aspberg, Marju Orho-Melander, Jan Nilsson, Ljubica Matic, Andreas Edsfeldt, Jiangming Sun, Eva Bengtsson
Summary: This study found that the expression of osteomodulin in human atherosclerotic plaques is associated with plaque calcification and plaque stability.