Article
Cell Biology
Dong Hoon Kang, Yerin Kim, Seongchun Min, Su Youn Lee, Ka Young Chung, In-Jeoung Baek, Kihwan Kwon, Hanjoong Jo, Sang Won Kang
Summary: Blood flows regulate the activation and inactivation of VEGFR2 through selective cysteine modifications, maintaining the structural and functional integrity of the arterial endothelium.
Article
Biochemistry & Molecular Biology
Alexander Mordhorst, Priyavathi Dhandapani, Susann Matthes, Valentina Mosienko, Michael Rothe, Mihail Todiras, Julie Self, Wolf-Hagen Schunck, Anja Schutz, Michael Bader, Natalia Alenina
Summary: Serotonin synthesis involves the hydroxylation of tryptophan, with most vertebrates having two known enzymes for this step. However, even in animals lacking these two enzymes, there is still some serotonin production, suggesting the presence of a third enzyme. The study found evidence that phenylalanine hydroxylase (PAH) may act as a third tryptophan hydroxylase in mammals, contributing to serotonin levels in the blood and potentially serving as a local source of serotonin in organs where other TPHs are not expressed.
Review
Pharmacology & Pharmacy
Glyn B. Steventon, Stephen C. Mitchell
Summary: S-Carboxymethyl-l-cysteine is a mucolytic agent used in the treatment of respiratory disorders, with multiple dose modeling indicating effective concentrations ranging from 1.30 to 8.40 μg/mL in the clinical setting. Pharmacodynamic studies reporting significant effects below approximately 10 μg/mL may occur in vivo, mainly through gene-related mechanisms. However, most events requiring levels much higher than achievable in clinical situations were only demonstrable in vitro.
Article
Chemistry, Multidisciplinary
Piyumi Dinusha Liyanage, Pabudi Weerathunge, Mandeep Singh, Vipul Bansal, Rajesh Ramanathan
Summary: Researchers have discovered that L-cysteine can act as an irreversible inhibitor to permanently block the catalytic activity of 2D NiO nanosheets. This breakthrough provides mechanistic insights into the catalytic inhibition process and demonstrates a highly specific sensor for detecting this biologically important molecule.
Article
Pharmacology & Pharmacy
Paulo R. Lino, Joao Leandro, Mariana Amaro, Lidia M. D. Goncalves, Paula Leandro, Antonio J. Almeida
Summary: Enzyme nanoencapsulation using a chitosan-based delivery system successfully encapsulated a complex human metabolic enzyme, providing a significant framework for enzyme formulation.
Article
Food Science & Technology
Takashi Hashimoto, Shoji Yoshioka, Shiroh Iwanaga, Kazuki Kanazawa
Summary: The study found that AITC can be metabolized into NAC-AITC, which exhibits anti-malarial activity against malaria parasites. These findings suggest that consuming AITC-containing foods may help prevent malaria.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Taku Mizutani, Ryotaro Hara, Takayuki Iihoshi, Shoko Kozono, Michiki Takeuchi, Makoto Hibi, Satomi Takahashi, Makoto Ueda, Jun Ogawa
Summary: This study found that E. coli has the ability to synthesize S-allyl-L-cysteine (SAC) through the enzyme tryptophanase (TnaA). TnaA also showed the ability to synthesize a wide range of S-substituted cysteines with various chains or rings. These findings have important implications for the development of new processes for synthesizing valuable S-substituted cysteine derivatives.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Oladipupo A. Aregbesola, Ajit Kumar, Mduduzi P. Mokoena, Ademola O. Olaniran
Summary: The metabolically promiscuous pentachlorophenol (PCP) hydroxylating Phe4MO, represented as CpsB, was detected and cloned from the genome of Bacillus tropicus strain AOA-CPS1. The enzyme functions as a PCP dehalogenase or PCP-4-monooxygenase, showing optimal activity at 30 degrees C and pH 7.0.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Anthony L. Su, Lawrence H. Lash, Rita Loch-Caruso
Summary: DCVC, a metabolite of TCE, can induce oxidative stress and apoptosis in placental cells. The study found that NAC and AOAA had no significant impact on DCVC toxicity, but CCBL activity and CYP3A4 expression may influence DCVC toxicity. This suggests that the toxicity of DCVC may be related to cellular metabolism.
Article
Chemistry, Physical
Cui Lu, Sheng Zhang, Wei Song, Jia Liu, Xiulai Chen, Liming Liu, Jing Wu
Summary: The study successfully achieved efficient production of D-phenylalanine from L-phenylalanine through the engineering of a tri-enzymatic cascade system, providing a new bio-based approach for industrial production of D-phenylalanine from cheap amino acids.
Article
Chemistry, Inorganic & Nuclear
Nikolina Kalcec, Antonio Ljulj, Lucija Bozicevic, Valerije Vrcek, Domenico Marson, Sabrina Pricl, Frances Separovic, Ivana Vinkovic Vrcek
Summary: Gold nanoparticles have potential applications in biomedicine as diagnostic tools and drug delivery agents. The research findings show that the final form of catechol on the nanosurface of gold nanoparticles depends on the molar ratio of gold used for preparation. The study highlights the importance of a combined experimental and computational approach for reliable characterization of binding events on the surface of gold nanoparticles.
INORGANIC CHEMISTRY
(2022)
Article
Medicine, General & Internal
Fernando Andrade, Ainara Cano, Maria Unceta Suarez, Arantza Arza, Ana Vinuesa, Leticia Ceberio, Nuria Lopez-Osle, Gorka de Frutos, Raquel Lopez-Oceja, Elena Aznal, Domingo Gonzalez-Lamuno, Javier de las Heras
Summary: The study found that PKU patients have higher urine PAG levels than healthy controls, and there is a significant correlation between urine PAG levels and circulating Phe levels in patients with HPA, suggesting the potential role of urine PAG as a non-invasive biomarker in PKU diagnosis.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Environmental Sciences
Fang-Fang Zhao, Xiao-Li Wang, Ya-Ting Lei, Hong-Qiu Li, Zhi-Ming Li, Xiao-Xiao Hao, Wei-Wei Ma, Yong-Hui Wu, Sheng-Yuan Wang
Summary: Acrylamide, a neurotoxic and carcinogenic compound, is widely present in fried foods and cigarettes. Its intake assessment through dietary questionnaires is limited due to confounding factors. This review focuses on the levels of AAMA, a urinary metabolite of acrylamide in humans, its association with diseases, and research gaps and future needs. Studies from eight countries showed higher urinary AAMA levels in smokers and children, with the highest levels found in the population from Spain. AAMA is associated with various diseases, especially cardiovascular diseases, making it an important biomarker for internal acrylamide exposure and setting allowable intake in foods.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Olivia M. Manley, Haoyu Tang, Shan Xue, Yisong Guo, Wei-chen Chang, Thomas M. Makris
Summary: BesC is a member of an emerging family of diiron enzymes that catalyze an unusual type of carbon-carbon cleavage reaction. It activates O-2 in a substrate-gated manner to generate a diferric-peroxo intermediate, initiating a unique reaction trajectory by cleaving the C4-H bond as the rate-limiting step in a single turnover reaction.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biotechnology & Applied Microbiology
Tao Wu, Xiaoqing Mu, Yuyan Xue, Yan Xu, Yao Nie
Summary: This study successfully enhanced the efficiency of l-HPA synthesis by engineering PheDH through a structure-guided steric hindrance approach, resulting in the identification of seven superior mutants. The optimal triple-site mutant, V309G/L306V/V144G, showed significantly higher catalytic activity compared to the wild type, achieving the highest substrate conversion rates to date. Docking simulations revealed changes in substrate-binding volume and enzyme tunnels, indicating the potential industrial applications of this mutant for l-HPA synthesis.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Review
Pharmacology & Pharmacy
Glyn Steventon
Review
Pharmacology & Pharmacy
Stephen C. Mitchell, Glyn B. Steventon
Editorial Material
Pharmacology & Pharmacy
Stephen Mitchell, Glyn Steventon
Article
Biochemistry & Molecular Biology
M. Tamayo, A. Fulgencio-Covian, J. A. Navarro-Garcia, A. Val-Blasco, G. Ruiz-Hurtado, M. Gil-Fernandez, L. Martin-Nunes, J. A. Lopez, L. R. Desviat, C. Delgado, E. Richard, M. Fernandez-Velasco
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2020)
Article
Genetics & Heredity
Diana Gallego, Fatima Leal, Alejandra Gamez, Margarita Castro, Rosa Navarrete, Obdulia Sanchez-Lijarcio, Isidro Vitoria, Maria Bueno-Delgado, Amaya Belanger-Quintana, Ana Morais, Consuelo Pedron-Giner, Inmaculada Garcia, Jaume Campistol, Rafael Artuch, Carlos Alcaide, Veronica Cornejo, David Gil, Raquel Yahyaoui, Lourdes R. Desviat, Magdalena Ugarte, Aurora Martinez, Belen Perez
Article
Medical Laboratory Technology
Alejandro Fulgencio-Covian, Esmeralda Alonso-Barroso, Adam J. Guenzel, Ana Rivera-Barahona, Magdalena Ugarte, Belen Perez, Michael A. Barry, Celia Perez-Cerda, Eva Richard, Lourdes R. Desviat
TRANSLATIONAL RESEARCH
(2020)
Article
Genetics & Heredity
Alicia Hillert, Yair Anikster, Amaya Belanger-Quintana, Alberto Burlina, Barbara K. Burton, Carla Carducci, Ana E. Chiesa, John Christodoulou, Maja Dordevic, Lourdes R. Desviat, Aviva Eliyahu, Roeland A. F. Evers, Lena Fajkusova, Francois Feillet, Pedro E. Bonfim-Freitas, Maria Gizewska, Polina Gundorova, Daniela Karall, Katya Kneller, Sergey Kutsev, Vincenzo Leuzzi, Harvey L. Levy, Uta Lichter-Konecki, Ania C. Muntau, Fares Namour, Mariusz Oltarzewski, Andrea Paras, Belen Perez, Emil Polak, Alexander Polyakov, Francesco Porta, Marianne Rohrbach, Sabine Scholl-Burgi, Norma Specola, Maja Stojiljkovic, Nan Shen, Luiz C. Santana-da Silva, Anastasia Skouma, Francjan van Spronsen, Vera Stoppioni, Beat Thony, Friedrich K. Trefz, Jerry Vockley, Youngguo Yu, Johannes Zschocke, Georg F. Hoffmann, Sven F. Garbade, Nenad Blau
AMERICAN JOURNAL OF HUMAN GENETICS
(2020)
Article
Biochemistry & Molecular Biology
A. Vilas, P. Yuste-Checa, D. Gallego, L. R. Desviat, M. Ugarte, C. Perez-Cerda, A. Gamez, B. Perez
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2020)
Article
Biochemistry & Molecular Biology
Esmeralda Alonso-Barroso, Belen Perez, Lourdes Ruiz Desviat, Eva Richard
Summary: Propionic acidemia (PA) is a common life-threatening organic acidemia caused by mutations in PCCA or PCCB genes. Cardiac alterations are a major cause of mortality in PA patients. Study using iPSC derived cardiomyocytes from a PA patient showed characteristics such as reduced oxygen consumption and endoplasmic reticulum stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Alejandro Fulgencio-Covian, Mar Alvarez, Barry A. Pepers, Aristides Lopez-Marquez, Magdalena Ugarte, Belen Perez, Willeke M. C. van Roon-Mom, Lourdes R. Desviat, Eva Richard
STEM CELL RESEARCH
(2020)
Review
Cardiac & Cardiovascular Systems
Kyung Chan Park, Steve Krywawych, Eva Richard, Lourdes R. Desviat, Pawel Swietach
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2020)
Review
Medicine, Research & Experimental
Suzan M. Hammond, Annemieke Aartsma-Rus, Sandra Alves, Sven E. Borgos, Ronald A. M. Buijsen, Rob W. J. Collin, Giuseppina Covello, Michela A. Denti, Lourdes R. Desviat, Lucia Echevarria, Camilla Foged, Gisela Gaina, Alejandro Garanto, Aurelie T. Goyenvalle, Magdalena Guzowska, Irina Holodnuka, David R. Jones, Sabine Krause, Taavi Lehto, Marisol Montolio, Willeke Van Roon-Mom, Virginia Arechavala-Gomeza
Summary: The field of nucleic acid-based therapeutics has seen rapid development in recent years, with the main challenge being delivery to target tissues. The adoption of delivery technologies, such as conjugates or nanoparticles, has been a game changer for many therapeutic indications.
EMBO MOLECULAR MEDICINE
(2021)
Review
Pharmacology & Pharmacy
Glyn B. Steventon, Stephen C. Mitchell
Summary: S-Carboxymethyl-l-cysteine is a mucolytic agent used in the treatment of respiratory disorders, with multiple dose modeling indicating effective concentrations ranging from 1.30 to 8.40 μg/mL in the clinical setting. Pharmacodynamic studies reporting significant effects below approximately 10 μg/mL may occur in vivo, mainly through gene-related mechanisms. However, most events requiring levels much higher than achievable in clinical situations were only demonstrable in vitro.
Article
Biochemistry & Molecular Biology
Ainhoa Martinez-Pizarro, Fatima Leal, Lise Lolle Holm, Thomas K. Doktor, Ulrika S. S. Petersen, Maria Bueno, Beat Thony, Belen Perez, Brage S. Andresen, Lourdes R. Desviat
Summary: We report the identification of two new splicing variants of 6-pyruvoyl-tetrahydropterin synthase in a patient with tetrahydrobiopterin deficiency. These variants were found to cause the inclusion of abnormal pseudoexons, leading to hyperphenylalaninemia and monoamine neurotransmitter deficiency. Antisense oligonucleotides were designed to correct the aberrant pseudoexon inclusion, demonstrating the therapeutic potential of this approach.
NUCLEIC ACID THERAPEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Mar Alvarez, Pedro Ruiz-Sala, Belen Perez, Lourdes Ruiz Desviat, Eva Richard
Summary: Propionic acidemia (PA) disorder primarily affects the heart and leads to cardiac complications due to the accumulation of toxic metabolites. Cardiomyocytes generated from induced pluripotent stem cells (iPSCs) derived from a PA patient were used to study microRNAs (miRNAs) profiles and cellular pathways associated with PA cardiac phenotypes. The study identified upregulated cardiac-enriched miRNAs and alterations in various cellular pathways, providing insights into the molecular mechanisms of this rare disease and potential therapeutic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)