Article
Hematology
Daniel J. Wong, Diane D. Park, Simon S. Park, Carolyn A. Haller, Jiaxuan Chen, Erbin Dai, Liying Liu, Appi R. Mandhapati, Pradheep Eradi, Bibek Dhakal, Walter J. Wever, Melinda Hanes, Lijun Sun, Richard D. Cummings, Elliot L. Chaikof
Summary: P-G6, a potent P-selectin inhibitor, blocks interactions between platelets and leukocytes in the process of venous thrombosis, reducing thrombus formation without affecting hemostasis.
Article
Oncology
Tomoyuki Nakano, Kenji Fujimoto, Arata Tomiyama, Masamichi Takahashi, Takamune Achiha, Hideyuki Arita, Daisuke Kawauchi, Mami Yasukawa, Kenkichi Masutomi, Akihide Kondo, Yoshitaka Narita, Taketoshi Maehara, Koichi Ichimura
Summary: Eribulin demonstrated strong inhibitory effects on cell proliferation, induced apoptosis, and suppressed cell migration in malignant meningioma cell lines, leading to significantly prolonged survival in orthotopic xenograft mouse models.
Review
Endocrinology & Metabolism
Niloofar Dehdari Ebrahimi, Alireza Sadeghi, Sara Shojaei-Zarghani, Mohammad Amin Shahlaee, Erfan Taherifard, Zahra Rahimian, Zahra Eghlidos, Negar Azarpira, Ali Reza Safarpour
Summary: This study systematically reviewed the protective effects of melatonin on rodent testicular tissue exposed to anti-cancer stressors. The meta-analysis of 38 studies found that melatonin therapy significantly improved sperm quantity and quality, reproductive hormone levels, weight-related characteristics, and reduced oxidative markers in testicular tissues of male rodents. However, there were biases and heterogeneity in the included studies.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Joseph C. Watso, Luke N. Belval, Frank A. Cimino, Bonnie D. Orth, Joseph M. Hendrix, Mu Huang, Elias Johnson, Josh Foster, Carmen Hinojosa-Laborde, Craig G. Crandall
Summary: In this randomized, crossover, placebo-controlled trial, it was found that tolerance to simulated hemorrhage was lower after low-dose morphine administration. Such reductions in hemorrhagic tolerance were observed without differences in MSNA burst frequency responses between morphine and placebo trials. These data, the first to be obtained in conscious humans, demonstrate that low-dose morphine reduces hemorrhagic tolerance. Thus, morphine is not an ideal analgesic for a hemorrhaging individual in the prehospital setting.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Engineering, Biomedical
Marianne Wauchop, Naimeh Rafatian, Yimu Zhao, Wenliang Chen, Mark Gagliardi, Stephane Masse, Brian J. Cox, Patrick Lai, Timothy Liang, Shira Landau, Stephanie Protze, Xiao Dong Gao, Erika Yan Wang, Kelvin Chan Tung, Zachary Laksman, Rick Xing Ze Lu, Gordon Keller, Kumaraswamy Nanthakumar, Milica Radisic, Peter H. Backx
Summary: To better understand SCN5A-related cardiomyopathies, ventricular cardiomyocytes were generated from induced pluripotent stem cells of a patient with dilated cardiomyopathy and the R222Q mutation. Heart-on-a-chip biowires were created to study the effects of the mutation on myocardial maturation. The R222Q mutation not only altered cardiac electrophysiology and Na+ current, but also caused disruptions in sarcomere structure and mechanical destabilization.
Article
Gastroenterology & Hepatology
Zichao Yu, Yu Wang, Fang Zhang, Rui Ma, Xiaoyu Yang, Kun Yang, Ai Mi, Liyuan Ran, Yingjie Wu
Summary: Both GH and gut microbiota have significant impacts on physiological processes, and this study found that disruption of GHR in the liver affected gut microbiota and bile acid metabolism, while the influence on adipose tissue was limited. The findings suggest that liver GH signaling plays a crucial role in regulating gut microbiota and may contribute to microbiota-host interaction.
Article
Endocrinology & Metabolism
Erique Castro, Thayna S. Vieira, Tiago E. Oliveira, Milene Ortiz-Silva, Maynara L. Andrade, Caroline A. Tomazelli, Albert S. Peixoto, Cleyton R. Sobrinho, Mayara F. Moreno, Gustavo R. Gilio, Rafael J. Moreira, Raphael C. Guimaraes, Luiz A. Perandini, Patricia Chimin, Patricia Reckziegel, Eduardo H. Moretti, Alexandre A. Steiner, Mathieu Laplante, William T. Festuccia
Summary: Adipocyte-specific mTORC2 deficiency impairs BAT and iWAT total UCP-1 content and thermogenic capacity in cold-acclimated mice, but does not significantly affect glucose uptake and whole body energy expenditure. Compensation of the impaired UCP-1 content and thermogenic capacity in BAT and iWAT by activation of muscle shivering was observed in cold-acclimated mice.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Cell Biology
Wen-juan Ma, Yukun Chen, Jian-hong Peng, Chaoming Tang, Ling Zhang, Min Liu, Shanshan Hu, Haineng Xu, Hua Tan, Yangkui Gu, Zhi-zhong Pan, Gong Chen, Zhong-guo Zhou, Rong-xin Zhang
Summary: According to the EPOC study, chemotherapy improves 5-year disease-free survival in stage IV colon cancer patients by 8.1%. This study found that DENND2D expression is associated with better prognosis in these patients, and DENND2D-negative patients are more likely to benefit from neoadjuvant chemotherapy.
CELL DEATH & DISEASE
(2022)
Article
Multidisciplinary Sciences
Thomas J. Sproule, Robert Y. Wilpan, John J. Wilson, Benjamin E. Low, Yudai Kabata, Tatsuo Ushiki, Riichiro Abe, Michael V. Wiles, Derry C. Roopenian, John P. Sundberg
Summary: The study demonstrates the possibility of genetic modification of EB symptoms using the Lamc2jeb mouse model and identifies Col17a1 and other genetic loci as modifiers. Furthermore, CRISPR/Cas9 technology was used to alter a specific isoform of the dystonin gene in mice and validated a genetic difference as a modifier of EB. The study also reveals the potential of using mice with pinnae removed as a test bed for studying EB disease and treatment.
Article
Gastroenterology & Hepatology
Wenting Chen, Mai Imasaka, Hideaki Iwama, Hiroshi Nishiura, Masaki Ohmuraya
Summary: This study found that double deletion of Ctsb and Ctsl in the mouse pancreas altered intrapancreatic trypsin activity but did not affect the severity of acute pancreatitis and inflammatory response.
Article
Neurosciences
Thierno M. Bah, Elyse M. Allen, Manuel Garcia-Jaramillo, Ruby Perez, Yalda Zarnegarnia, Catherine M. Davis, Madeline B. Bloom, Armando A. Magana, Jaewoo Choi, Gerd Bobe, Martin M. Pike, Jacob Raber, Claudia S. Maier, Nabil J. Alkayed
Summary: GPR39 plays a role in spatial memory retention and protects against HFD-induced cognitive impairment by modulating inflammation and AA-derived oxylipins. The results suggest that GPR39 and oxylipin pathways may serve as therapeutic targets in VCI.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Neurosciences
Christiaan F. M. Huffels, Lana M. Osborn, Natalie L. M. Cappaert, Elly M. Hol
Summary: The study found that calcium signaling in astrocytes in Alzheimer's disease is affected by both aging and amyloid-beta pathology, but these changes do not alter astrocyte network activity. These alterations in calcium dynamics may help us understand the neurophysiological changes that lead to cognitive decline and dementia.
JOURNAL OF NEUROSCIENCE RESEARCH
(2022)
Article
Endocrinology & Metabolism
Kalman Bodis, Saida Breuer, Assja Crepzia-Pevzner, Oana-Patricia Zaharia, Martin Schoen, Nina Saatmann, Delsi Altenhofen, Christian Springer, Julia Szendroedi, Robert Wagner, Hadi Al-Hasani, Michael Roden, Dominik Pesta, Alexandra Chadt, GDS Grp
Summary: Exercise training can induce beiging of adipose tissue and improve glucose homeostasis and mitochondrial function. This study investigated the association between beiging of subcutaneous adipose tissue (scWAT) and physical fitness in healthy humans and recent-onset type 2 diabetes, as well as the effect of voluntary running wheel intervention on beiging in mice. The results showed that higher physical fitness was associated with higher expression levels of the beiging marker CD137 in humans and mice on a high-fat diet. However, individuals with recent-onset type 2 diabetes showed an impaired adipose tissue-specific response to physical activity.
DIABETES OBESITY & METABOLISM
(2023)
Article
Biochemistry & Molecular Biology
Pinar Obakan Yerlikaya, Kaan Adacan, Ayse Karatug Kacar, Ajda Coker Gurkan, Elif Damla Arisan
Summary: This study evaluated the anti-tumoral effect of epibrassinolide in colon cancer cell lines and in a mouse model, and found that it inhibited tumor growth by causing cell cycle arrest.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Lin Gui, Shaoyan Liu, Ye Zhang, Yuankai Shi
Summary: The study presented a case of ATC patient who achieved significant tumor shrinkage and sustained remission period of 18.3 months using a combination of immunotherapy and anti-angiogenesis agent, providing a novel therapeutic option for ATC patients.
ONCOTARGETS AND THERAPY
(2021)
Review
Endocrinology & Metabolism
Chen-Che Jeff Huang, Yuan Kang
JOURNAL OF ENDOCRINOLOGY
(2019)
Article
Endocrinology & Metabolism
Tulay Guran, Cengiz Kara, Melek Yildiz, Eda C. Bitkin, Goncagul Haklar, Jen-Chieh Lin, Mehmet Keskin, Lise Barnard, Ahmet Anik, Gonul Catli, Ayla Guven, Birgul Kirel, Filiz Tutunculer, Hasan Onal, Serap Turan, Teoman Akcay, Zeynep Atay, Gulay C. Yilmaz, Jamala Mamadova, Azad Akbarzade, Onder Sirikci, Karl-Heinz Storbeck, Tugba Baris, Bon-Chu Chung, Abdullah Bereket
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2020)
Article
Multidisciplinary Sciences
Qiongxia Lyu, Huifei Sophia Zheng, Karly Laprocina, Chen-Che Jeff Huang
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2020)
Article
Endocrinology & Metabolism
Qiongxia Lyu, Hui Wang, Yuan Kang, Xiangmeng Wu, Huifei Sophia Zheng, Karly Laprocina, Kristina Junghans, Xinxin Ding, Chen-Che Jeff Huang
Article
Behavioral Sciences
Chad D. Foradori, Laci Mackay, Chen-Che J. Huang, Robert J. Kemppainen
Summary: Rasd1, induced by dexamethasone, is mainly located in corticotrophs in the pituitary, and its expression is upregulated in response to exogenous Dex.
STRESS-THE INTERNATIONAL JOURNAL ON THE BIOLOGY OF STRESS
(2021)
Editorial Material
Cell Biology
Yann Gibert, Bon-Chu Chung
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2021)
Review
Cell Biology
Chen-wei Hsu, Bon-chu Chung
Summary: Fish gonads develop differently from mammalian gonads, mainly due to species-specific factors such as the presence of the gsdf gene in teleosts but its absence in many tetrapods. The gsdf transcript is primarily expressed in gonadal somatic cells, with variable functions related to germ cell proliferation, testicular formation, ovarian development, and sex determination in different fish species. The common and diverse expression, regulation, and functions of gsdf among different fish species are summarized with respect to evolution.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Meng-Chun Monica Shih, Chen-Che Jeff Huang, Hsueh-Ping Chu, Nai-Chi Hsu, Bon-Chu Chung
Summary: The study reveals that fetal energy homeostasis is established before birth, and fetal steroids are necessary for regulating glycogen synthesis, hepatic gluconeogenesis, and insulin secretion during the fetal stage.
Article
Endocrinology & Metabolism
Konrad Patyra, Christoffer Lof, Holger Jaeschke, Hendrik Undeutsch, Huifei Sophia Zheng, Sofia Tyystjarvi, Kamila Pulawska, Milena Doroszko, Marcin Chrusciel, Britt-Marie Loo, Riikka Kurkijarvi, Fu-Ping Zhang, Chen-Che Jeff Huang, Claes Ohlsson, Andreina Kero, Matti Poutanen, Jorma Toppari, Ralf Paschke, Nafis Rahman, Ilpo Huhtaniemi, Jarmo Jaaskelainen, Jukka Kero
Summary: This study found that congenital hypothyroidism and hyperthyroidism have functional effects on adrenal gland development, steroidogenic activity, and adrenal medulla.
Article
Multidisciplinary Sciences
Huifei Sophia Zheng, Chen-Che Jeff Huang
Summary: Cellular heterogeneity creates challenges for understanding tissue function at a transcriptome level, but using cell-type-specific RNAs can avoid these issues and enable powerful transcriptome analysis. The described protocol demonstrates how to isolate ribosome-bound RNAs from EGFP-expressing cells without cell sorting, providing a useful method for isolating cell-type-specific RNAs from complex tissues.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Endocrinology & Metabolism
Huifei Sophia Zheng, Jeffrey G. Daniel, Julia M. Salamat, Laci Mackay, Chad D. Foradori, Robert J. Kemppainen, Satyanarayana R. Pondugula, Ya-Xiong Tao, Chen-Che Jeff Huang
Summary: This study investigated the early transcriptomic responses to dexamethasone (Dex) stimulation in vitro and in vivo, showing that there were distinct differences in the gene expression patterns. The adrenal glands of mice treated with Dex for 1 hour showed a strong association with the development of neuronal cells, indicating a rapid response to Dex in the adrenal medulla. However, in the Y-1 cell line, only a small proportion of genes exhibited differential expression after 1 hour of Dex treatment, and their expression returned to basal levels after 24 hours.
ENDOCRINE CONNECTIONS
(2022)
Article
Biochemistry & Molecular Biology
Huifei Sophia Zheng, Yuan Kang, Qiongxia Lyu, Kristina Junghans, Courtney Cleary, Olivia Reid, Greer Cauthen, Karly Laprocina, Chen-Che Jeff Huang
Summary: Steroid hormones are synthesized through enzymatic reactions using cholesterol as the substrate. DHCR24 is a key enzyme controlling cholesterol synthesis. DHCR24 is highly expressed in the adrenal gland and its expression can be regulated by thyroid hormone treatment. The cellular expression pattern of DHCR24 in mouse adrenal glands is similar to that of the X-zone marker gene 20 alpha HSD in most developmental stages. DHCR24 is not essential for overall adrenal gland development or acute stress-induced corticosterone secretion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Julia Salamat, Kodye Abbott, Patrick Flannery, Mohammed Majrashi, Mohammed Almaghrabi, Manoj Govindarajulu, Sindhu Ramesh, Suneel Onteru, Maninder Sandey, Chen-Che Huang
Meeting Abstract
Biochemistry & Molecular Biology
Julia Salamat, Kodye Abbott, Patrick Flannery, Mohammed Majrashi, Mohammed Almaghrabi, Manoj Govindarajulu, Sindhu Ramesh, Maninder Sandey, Suneel Onteru, Chen-Che Huang