Journal
MOLECULAR ECOLOGY RESOURCES
Volume 11, Issue 2, Pages 415-417Publisher
WILEY
DOI: 10.1111/j.1755-0998.2010.02951.x
Keywords
birds; molecular sexing; noninvasive sampling; real-time PCR
Funding
- US Environmental Protection Agency
- US Geological Survey
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Polymerase chain reaction (PCR)-based methods to determine the sex of birds are well established and have seen few modifications since they were first introduced in the 1990s. Although these methods allowed for sex determination in species that were previously difficult to analyse, they were not conducive to high-throughput analysis because of the laboriousness of DNA extraction and gel electrophoresis. We developed a high-throughput real-time PCR-based method for analysis of sex in birds, which uses noninvasive sample collection and avoids DNA extraction and gel electrophoresis.
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