Journal
MOLECULAR ECOLOGY RESOURCES
Volume 9, Issue 1, Pages 393-398Publisher
WILEY
DOI: 10.1111/j.1755-0998.2008.02238.x
Keywords
cDNA; cross-species amplification; dipterocarp; expressed sequence tag; microsatellites; tropical tree species
Funding
- Ministry of Science, Technology and Innovation Malaysia (MOSTI) [02-03-10-SF0017]
- Ministry of Education, Culture, Sports, Science and Technology of Japan [18255010]
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Simple sequence repeats (SSRs) derived from expressed sequence tags (ESTs) are valuable markers because they represent transcribed regions and often transferable to related taxa. Here, we report the development and characterization of EST-SSRs from Shorea leprosula. Fifty-four sequences containing SSRs were identified in 2003 unigenes assembled from 3159 ESTs. Twenty-four EST-SSRs were developed, of which four gave multiple amplifications, five were found to be monomorphic and 15 showed polymorphism, with allele numbers ranging from two to 17 in a single Pasoh Forest Reserve population of 24 individuals. The observed and expected heterozygosities ranged from 0.05 to 0.91 and from 0.16 to 0.93, respectively. Cross-species transferability of the 15 loci to 36 species within Dipterocarpaceae revealed between four and 14 loci that gave positive amplification and 10 loci were found to be transferable to more than 15 species.
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