Journal
MOLECULAR ECOLOGY RESOURCES
Volume 9, Issue 3, Pages 946-948Publisher
WILEY-BLACKWELL PUBLISHING, INC
DOI: 10.1111/j.1755-0998.2008.02511.x
Keywords
genomic library; PCR; SSR enrichment
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Microsatellite loci were identified from the charcoal rot fungus (Macrophomina phaseolina). Primer pairs for 46 loci were developed, and of these, 13 were optimized and screened using genomic DNA from 55 fungal isolates collected predominantly from two soybean fields in Mississippi. Twelve of the optimized loci were polymorphic and the number of alleles per locus ranged from 6 to 22. These microsatellites will be useful in population and pathogenicity studies to correspond with development of potential disease-resistant soybean and other susceptible crops.
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