Journal
MOLECULAR ECOLOGY RESOURCES
Volume 9, Issue 3, Pages 862-864Publisher
WILEY
DOI: 10.1111/j.1755-0998.2009.02655.x
Keywords
database; microsatellite; mungbean; primer; SSR; Vigna radiata
Funding
- National Center for Genetic Engineering and Biotechnology (BIOTEC)
- Commission on Higher Education
- Ministry of Education, Thailand
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A novel set of microsatellite markers for mungbean [Vigna radiata (L.) Wilczek] was developed from the public sequence database. Seventy-eight primers were designed and evaluated for polymorphism among 22 cultivated accessions. Eight polymorphic loci detected two to three alleles per locus with an average of 2.25. The observed heterozygosity varied from 0.00 to 0.18, while the expected heterozygosity ranged from 0.09 to 0.46. Among them, all eight loci showed significant departuring from Hardy-Weinberg equilibrium, while four pairs of loci displayed significant pairwise linkage disequilibrium values. All eight loci except DMB-SSR1 showed heterozygote deficiency.
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