Journal
MOLECULAR ECOLOGY RESOURCES
Volume 9, Issue 1, Pages 134-136Publisher
WILEY
DOI: 10.1111/j.1755-0998.2008.02367.x
Keywords
hybridization; Lahontan cutthroat trout; microsatellite; Oncorhynchus clarki henshawi; threatened species
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Thirteen newly developed tri- and tetranucleotide repeat microsatellite markers were developed for Lahontan cutthroat trout (Oncorhynchus clarki henshawi), a threatened subspecies endemic to the Lahontan hydrographic basin in the western USA. These loci are highly polymorphic with five to 30 alleles per locus and observed heterozygosities ranging from 0.4 to 0.7. Cross-species amplification of these markers was most successful in the closely related rainbow trout, Oncorhynchus mykiss, with only three loci amplifying in brown trout, Salmo trutta. Nonoverlapping allelic distributions for many of these loci among the six salmonid species screened suggest these markers may be useful for hybrid determination.
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