Journal
MOLECULAR ECOLOGY RESOURCES
Volume 9, Issue 4, Pages 1145-1147Publisher
WILEY
DOI: 10.1111/j.1755-0998.2009.02585.x
Keywords
enrichment; heterozygote deficit; microsatellites; Paracentrotus; sea urchin
Funding
- Spanish Network of National Parks [119/2003]
- Spanish Ministry of Education and Science [CTM2007-66635]
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Nine polymorphic microsatellite loci were isolated and characterized for the edible common sea urchin Paracentrotus lividus. Loci were obtained from two genomic libraries enriched with different di-, tri- and tetranucleotides. Most microsatellites obtained were imperfect dinucleoticles. Allelic variation was screened for a total of 56 individuals from two populations from North-Western Mediterranean. Microsatellites showed a number of alleles ranging from 13 to 35. Linkage disequilibriurn was not detected between any pair of loci, and all loci showed a significant departure from Hardy-Weinberg equilibrium which is not likely to be the result of null alleles, suggesting that demographic features may be acting upon this commercially interesting species.
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