Journal
MOLECULAR ECOLOGY RESOURCES
Volume 8, Issue 4, Pages 910-912Publisher
WILEY
DOI: 10.1111/j.1755-0998.2008.02113.x
Keywords
ascomycete fungus; banana; black sigatoka; microsatellite; Mycosphaerella fijiensis
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Fourteen polymorphic microsatellite markers were developed for Mycosphaerella fijiensis, a fungus causing the black sigatoka disease in banana. The sequenced genome of M. fijiensis was screened for sequences with single sequence repeats (SSRs) using a Per1 script. Fourteen SSR loci, evaluated on 48 M. fijiensis isolates from Hawaii, were identified to be highly polymorphic. These markers revealed two to 19 alleles, with an average of 6.43 alleles per locus. The estimated gene diversity ranged from 0.091 to 0.930 across the 14 microsatellite loci. The SSR markers developed would be useful for population genetics studies of M. fijiensis.
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