4.7 Article

Microsatellite markers for the fungal banana pathogens Mycosphaerella fijiensis, Mycosphaerella musicola and Mycosphaerella eumusae

Journal

MOLECULAR ECOLOGY RESOURCES
Volume 8, Issue 5, Pages 1121-1125

Publisher

WILEY
DOI: 10.1111/j.1755-0998.2008.02180.x

Keywords

leaf spot diseases of banana; microsatellites; multiplex PCR; Mycosphaerella eumusae; Mycosphaerella fijiensis; Mycosphaerella musicola

Funding

  1. Genoscope (Evry, France)

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We developed a total of 50 microsatellite markers for the three fungal pathogens causing the most important leaf spot diseases of banana: 32 loci for Mycosphaerella fijiensis are presented, and nine loci each for Mycosphaerella musicola and Mycosphaerella eumusae. All these loci were polymorphic within each species on a sample of isolates collected from various locations around the world. Within M. fijiensis and M. musicola, most of the loci tested (> 80%) in a sample of isolates from a single location in Cameroon were also polymorphic. Multiplex polymerase chain reaction systems were developed with 15 loci for M. fijiensis.

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