Journal
MOLECULAR ECOLOGY RESOURCES
Volume 8, Issue 6, Pages 1303-1306Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1755-0998.2008.02137.x
Keywords
Cynoglossus semilaevis; fosmid library; half-smooth tongue sole; microsatellite
Funding
- '863' Hi-Tech Research and Development Program of China [2006AA10A404]
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Microsatellite markers were developed from a fosmid library of female half-smooth tongue sole, Cynoglossus semilaevis. Three hundred eighty-four clones were randomly selected to sequence (double strand reading), and 168 sequences in 143 clones were found to contain microsatellites. Of the 101 primer pairs designed, 64 gave polymorphic polymerase chain reaction products. Based on characterization with 36 individuals, the number of alleles ranged from two to nine. The values of observed and expected heterozygosities varied from 0.06 to 1.00 and from 0.05 to 0.94, respectively. These markers have the potential as tools for population structure evaluation, ecological analyses and linkage map construction.
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