Journal
MOLECULAR ECOLOGY RESOURCES
Volume 8, Issue 6, Pages 1429-1435Publisher
BLACKWELL PUBLISHING
DOI: 10.1111/j.1755-0998.2008.02251.x
Keywords
expressed sequence tag; high-throughput; seagrass; single nucleotide polymorphism; SNaPshot; Zostera marina
Funding
- University of Groningen Breedtestrategie
- 'Deutsche Forschungsgemeinschaft' [Re 1108/7]
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We characterized 37 single nucleotide polymorphism (SNP) makers for eelgrass Zostera marina. SNP markers were developed using existing EST (expressed sequence tag)-libraries to locate polymorphic loci and develop primers from the functional expressed genes that are deposited in The ZOSTERA database (V1.2.1). SNP loci were genotyped using a single-base-extension approach which facilitated high-throughput genotyping with minimal optimization time. These markers show a wide range of variability among 25 eelgrass populations and will be useful for population genetic studies including evaluation of population structure, historical demography, and phylogeography. Potential applications include haplotype inference of physically linked SNPs and identification of genes under selection for temperature and desiccation stress.
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