Article
Multidisciplinary Sciences
Zhou Fang, Junjian Chen, Ye Zhu, Guansong Hu, Haoqian Xin, Kunzhong Guo, Qingtao Li, Liangxu Xie, Lin Wang, Xuetao Shi, Yingjun Wang, Chuanbin Mao
Summary: The study demonstrates the use of a click immobilization strategy to create gradients of two different functional peptides on a surface for rapid optimization. Optimizing the concentration of different functional peptides on a surface can be a complex process.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Physical
Hyeon Woo Kim, Joo Hyeong Han, Hyunseok Ko, Tuhin Samanta, Dong Geon Lee, Dong Won Jeon, Woongchan Kim, Yong-Chae Chung, Won Bin Im, Sung Beom Cho
Summary: Exploring the vast and veiled chemical spaces through synthesis is crucial in solid-state materials. This study focuses on navigating metal halides, particularly the coexistence of perovskites and their derivatives, which has restricted their exploration. By combining high-throughput density functional theory calculations and machine learning, the researchers systematically explore inorganic halide perovskite derivatives, charting the chemical spaces and predicting their electrical properties and thermal stability. The successful synthesis of new Cs3LuCl6 and its application in white-light-emitting diodes showcase the potential practical applications of inorganic metal halides.
ACS ENERGY LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Michael John Craig, Max Garcia-Melchor
Summary: The study revealed the universal scaling relationship for water oxidation catalysts, described routes to circumvent this relationship, and confirmed the ascendency of Ru catalysts for the reaction.
CELL REPORTS PHYSICAL SCIENCE
(2021)
Article
Biophysics
Yifan Wu, Longjiao Zhu, Shuting Li, Huashuo Chu, Xinxin Wang, Wentao Xu
Summary: This review presents a rational riboswitch biosensor design that combines computational and hybrid methods. The steps of rational design, screening, tailoring, and selection are discussed, along with the applications of riboswitch biosensors in molecular detection and biological imaging. The future development and applications of ideal riboswitch biosensors are also discussed.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Min Li, Huijuan Yu, Yiran Li, Xin Li, Shiqing Huang, Xiaogang Liu, Gaoqi Weng, Lei Xu, Tingjun Hou, Dong-Sheng Guo, Yi Wang
Summary: By utilizing supramolecular chemistry and artificial peptide library, a turn-on fluorescent sensing system for intracellular imaging of histone deacetylase 1 (HDAC1) was developed, allowing specific and highly sensitive detection, and a novel HDAC1 down-regulator was identified through high-throughput screening.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Lianping Wu, Tian Guo, Teng Li
Summary: A data-driven high-throughput design principle is proposed to evaluate the stability and activity of double-atom catalysts (DACs) for oxygen evolution and oxygen reduction reactions. This approach not only yields remarkable prediction precision but also significantly reduces the screening time compared to pure density functional theory calculations.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Review
Pharmacology & Pharmacy
Michal Vasina, Jan Velecky, Joan Planas-Iglesias, Sergio M. Marques, Jana Skarupova, Jiri Damborsky, David Bednar, Stanislav Mazurenko, Zbynek Prokop
Summary: Therapeutic enzymes are valuable biopharmaceuticals that have found successful applications in various biomedical fields. The integration of computational approaches and high-throughput experimental technologies has greatly accelerated the identification and engineering of catalytic therapeutic agents, leading to the discovery of new enzymes and the improvement of their properties.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Engineering, Biomedical
Yanxi Liu, Sarah Bertels, Markus Reischl, Ravindra Peravali, Martin Bastmeyer, Anna A. Popova, Pavel A. Levkin
Summary: Human induced pluripotent stem cells (hiPSCs) are crucial for disease modeling, drug discovery, and personalized medicine. Animal-derived materials hinder applications of hiPSCs in medical fields. Thus, novel and well-defined substrate coatings capable of maintaining hiPSC pluripotency are important for advancing biomedical applications of hiPSCs. Here a miniaturized droplet microarray (DMA) platform is used to investigate the ability of 11 well-defined proteins and their combinations to maintain pluripotency of hiPSCs when applied as a surface coating. Ten protein group coatings are identified, which promote significantly higher NANOG expression of hiPSCs compared to Matrigel coating. The DMA platform offers a potential solution for miniaturized high-throughput screening of scarce cells or expensive materials.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Biochemical Research Methods
Hang Zhang, Mengjing Xu, Haofeng Li, Xiaohan Mai, Jiawei Sun, Lan Mi, Jiong Ma, Xiangdong Zhu, Yiyan Fei
Summary: This study presents a series of optimization methods that greatly reduce the scanning time of OI-RD microscope images, resulting in a 10 times increase in detection speed. This makes the OI-RD microscope suitable for ultra-high throughput screening applications.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Chemistry, Multidisciplinary
Zhou Fang, Meng Zhang, Huaiming Wang, Junjian Chen, Haipeng Yuan, Mengyao Wang, Silin Ye, Yong-Guang Jia, Fu Kit Sheong, Yingjun Wang, Lin Wang
Summary: In this work, a strategy of biomaterial rational design is developed by combining gradient surface generation with statistical learning. Parameter combinations are screened in a high-throughput fashion, and the optimal conditions beyond the experimentally tested range are extrapolated from the models. An unprecedented ternary functionalized surface is designed for orthopedic implant with optimal osteogenic, angiogenic, and neurogenic activities, which is confirmed in vitro and in vivo.
ADVANCED MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Hyeonsuk Yoo, Ruth M. Muthoka, Xiangyu Zhang, Yongjin Lee
Summary: This study proposes a data-driven high-throughput screening approach coupled with molecular dynamics simulations for designing high-performance co-polymerized aramid fibers. By screening a large number of potential monomers, 31 promising and novel diamine monomers for aramid copolymers were successfully identified.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Materials Science, Biomaterials
Hongye Hao, Junjie Huang, Ping Liu, Yunfan Xue, Jing Wang, Kefeng Ren, Qiao Jin, Jian Ji, A. Greiner, S. Agarwal
Summary: In this study, a microarray-based high-throughput platform with biochemistry gradients was developed using thiol-ene click chemistry on a poly(limonene carbonate) substrate. The grafting density of sulfhydryl molecules like PEG and RGD on the substrate was well controlled by regulating the intensity of UV irradiation. The experiments on cell adhesion demonstrated that cell behaviors are highly determined by the RGD density on the substrate, suggesting a promising method to study the effect of biochemical signal density on cell behaviors.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Engineering, Chemical
Junpeng Yuan, Xianglong Liu, Min Li, Hui Wang
Summary: A class of potential materials for trapping benzene with ultra-high adsorption and outstanding selectivity was obtained through high-throughput computational screening. The microscopic mechanism of effective adsorption sites was explained using a local atomic environment tool. It is suggested that tuning the Si/Al ratio, introducing ionic liquids, and ion substitution can significantly improve the performance of potential adsorbents.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Engineering, Biomedical
Haijun Cui, Xueyuan Sun, Marcel Schilling, Christel Herold-Mende, Markus Reischl, Pavel A. A. Levkin, Anna A. A. Popova, Sevin Turcan
Summary: In order to address drug resistance and limited treatment options for recurrent gliomas with IDH1 mutations, a highly miniaturized screening of FDA-approved drugs was conducted using a high-throughput droplet microarray (DMA) platform. Over 20 drugs, including verteporfin (VP), were identified to significantly affect tumorsphere formation and viability. The study also revealed the nuclear pore complex as a potential target for drug development, suggesting a new mechanism of action for VP independent of its known effects on YAP1.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Matz Liebel, Irene Calderon, Nicolas Pazos-Perez, Niek F. van Hulst, Ramon A. Alvarez-Puebla
Summary: Large-scale SERS particle screening (LSSPS) is a fast multiplexed widefield screening approach that quantifies both Raman and Rayleigh scattering simultaneously, allowing for direct quantification of the fraction of SERS-active particles and unprecedented correlation of SERS activity with particle size.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Nanoscience & Nanotechnology
Sung Hee Ko, Divya Chandra, Wei Ouyang, Taehong Kwon, Pankaj Karande, Jongyoon Han
NATURE NANOTECHNOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Divya Chandra, Nitesh Sankalia, Imee Arcibal, Scott Banta, Donald Cropek, Pankaj Karande
Article
Crystallography
Sahar Farmanesh, Jihae Chung, Divya Chandra, Ricardo D. Sosa, Pankaj Karande, Jeffrey D. Rimer
JOURNAL OF CRYSTAL GROWTH
(2013)
Article
Biotechnology & Applied Microbiology
Divya Chandra, Steven Timmick, Chaz Goodwine, Nicholas Vecchiarello, Divya G. Shastry, Akshat Mullerpatan, John P. Trasatti, Steven Cramer, Pankaj Karande
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Akshat Mullerpatan, Divya Chandra, Erin Kane, Pankaj Karande, Steven Cramer
JOURNAL OF BIOTECHNOLOGY
(2020)
Article
Chemistry, Analytical
Xuanwen Li, Divya Chandra, Simon Letarte, Gregory C. Adam, Jonathan Welch, Rong-Sheng Yang, Shannon Rivera, Smaranda Bodea, Alex Dow, An Chi, Christopher A. Strulson, Douglas D. Richardson
Summary: Polysorbate is widely used in pharmaceutical formulation development to maintain the stability of biotherapeutic proteins, but its degradation can cause particle formation in drug products, posing a major quality issue and patient risk factor. This study successfully identified and confirmed active host cell enzymes responsible for polysorbate degradation using an activity-based protein profiling (ABPP) approach, bridging the gap between lipase abundance and activity.
ANALYTICAL CHEMISTRY
(2021)
Proceedings Paper
Cell & Tissue Engineering
D. Chandra, P. Karande
2011 IEEE 37TH ANNUAL NORTHEAST BIOENGINEERING CONFERENCE (NEBEC)
(2011)