Article
Chemistry, Physical
Yi-Chih Chiu, Mei-Chun Tseng, Chun-Hua Hsu
Summary: OAADPR, a signaling molecule, is involved in important cellular functions in yeast. Poa1p is an atypical 3''-O-acetyl-ADP-ribose deacetylase that plays a critical role in cellular nucleotide metabolism.
Article
Cell Biology
Arnold Tan, Awais Z. Younis, Alexander Evans, Jade V. Creighton, Clare Coveny, David J. Boocock, Craig Sale, Gareth G. Lavery, Amanda S. Coutts, Craig L. Doig
Summary: The PARP1 enzyme generates and applies ADP-Ribose, a post-translational modification. PARP1 plays important roles in genome maintenance, cellular identity, and energy homeostasis. In skeletal muscle cells, PARP1-mediated PARylation regulates the myogenic program and the muscle response to steroid hormones. PARylation is sensitive to glucose concentrations and glucocorticoids, which are crucial for muscle development and metabolism. PARP1 also influences the transcriptional activation of specific genes critical to skeletal muscle pathology, providing potential targets for selective glucocorticoid modulation.
CELL DEATH DISCOVERY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Michael S. Cohen
Summary: ADP-ribosylation is a crucial post-translational modification, involving MAR and PAR. Researchers have identified distinct MAR and PAR readers using a chemical biology approach.
Article
Environmental Sciences
Zhuoying Zeng, Jingjing Lu, Desheng Wu, Ran Zuo, Yuxi Li, Haiyan Huang, Jianhui Yuan, Zhangli Hu
Summary: PARG silencing can inhibit BaP-induced carcinogenesis by downregulating the expression of histone H2B subtypes. High levels of H2B predict poor survival in lung cancer patients, indicating its potential as a new prognostic biomarker. The expression of certain H2B subtypes is positively correlated with PARG expression in lung cancer tissues.
ENVIRONMENTAL TOXICOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Palmiro Poltronieri, Masanao Miwa, Mitsuko Masutani
Summary: ADP-ribosylation plays a crucial role in post-translational modifications of proteins, involving functions such as DNA repair, transcription, and cell signaling. Enzymes involved in monoADP-ribosylation/polyADP-ribosylation (MAR/PAR) cycling also play important roles in cancers, with potential applications in non-oncological diseases. The field is still actively uncovering novel enzymes at a rapid pace.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Nian-Da Yu, Bing Wang, Xin-Zhu Li, Hao-Zhen Han, Dongxiang Liu
Summary: SIRT1 is an important deacetylase with potential clinical applications. The novel mechanism of the selective activator CWR, which covalently bonds with OAADPr, provides a new perspective for designing SIRT1 activators.
Article
Biology
Sridevi Challa, Keun W. Ryu, Amy L. Whitaker, Jonathan C. Abshier, Cristel Camacho, W. Lee Kraus
Summary: In this study, PAR Trackers were developed and characterized as highly sensitive tools for detecting ADPR in various experimental and biological systems, providing greater temporal and spatial precision.
Article
Chemistry, Multidisciplinary
Qiang Liu, Gunnar Knobloch, Jim Voorneveld, Nico J. Meeuwenoord, Herman S. Overkleeft, Gijsbert A. van Der Marel, Andreas G. Ladurner, Dmitri Filippov
Summary: The translation discusses the significance of ADP-ribosylation and the chemical synthesis of linear ADP-ribose oligomers, as well as their affinity for ALC1. The study reveals the length-dependent binding manner of ADPr oligomers to ALC1, illustrating how the activity of this molecular machine is controlled by PAR.
Article
Multidisciplinary Sciences
Johannes Gregor Matthias Rack, Qiang Liu, Valentina Zorzini, Jim Voorneveld, Antonio Ariza, Kourosh Honarmand Ebrahimi, Julia M. Reber, Sarah C. Krassnig, Dragana Ahel, Gijsbert A. van der Marel, Aswin Mangerich, James S. O. McCullagh, Dmitri V. Filippov, Ivan Ahel
Summary: Poly(ADP-ribosyl)ation is a versatile posttranslational modification involved in regulating cellular processes. This study focuses on the mechanisms of PAR removal by PARG and ARH3, highlighting their differences in hydrolysis of linear and terminal ADP-ribose linkages.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Jie Li, Xiangxiang Liu, Bin Peng, Tingting Feng, Wen Zhou, Li Meng, Shanshan Zhao, Xiyuan Zheng, Chen Wu, Shian Wu, Xing Chen, Xingzhi Xu, Jianwei Sun, Jing Li
Summary: The study reveals that O-GlcNAc glycosylation promotes nuclear localization and chromatin association of PARG, enhances its recruitment to DNA damage sites, and inhibits the ADP-ribosylation of DDB1, stabilizing DDB1 and degrading its downstream target c-Myc. Additionally, the O-GlcNAc-deficient mutant PARG-S26A promotes hepatocellular carcinoma (HCC) in mouse xenograft models.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Maria Laura Kevorkian, Salome C. Vilchez Larrea, Silvia H. Fernandez Villamil
Summary: This study identifies the nuclear localization of TcPARP and its enrichment in the nucleolus of Trypanosoma cruzi. The study also reveals the presence of TcPARP in a thread-like structure connecting two dividing nuclei, and the detection of ADP-ribose polymers in this structure during mitosis. These findings shed light on new potential functions of TcPARP inside the nucleus and contribute to our understanding of its role in trypanosomatids beyond the well-described DNA damage response.
Article
Oncology
Sarah Johnson, Yaroslava Karpova, Danping Guo, Atreyi Ghatak, Dmitriy A. Markov, Alexei V. Tulin
Summary: Chemokines are highly expressed in the tumor microenvironment and play a critical role in tumorigenesis. Upregulation of PARG can inhibit tumor growth and reduce the expression of chemokines.
Article
Biochemistry & Molecular Biology
Mingming Yang, Chenliang Wang, Mi Zhou, Lei Bao, Yanan Wang, Ashwani Kumar, Chao Xing, Weibo Luo, Yingfei Wang
Summary: This study identifies the role of PARP-1 and KDM6B in alkylating agent-induced PARthanatic cancer cell death. KDM6B regulates the switch of PARP-1 signaling by inhibiting MGMT expression and DNA repair activity. These findings provide new insights into the epigenetic regulation of PARP-1 signaling and cell death mechanisms, and identify KDM6B as a biomarker for predicting cancer cell vulnerability to alkylating agent treatment.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Marion Schuller, Ivan Ahel
Summary: ADP-ribosylation is a post-translational modification of proteins that plays a crucial role in cellular processes and microbial pathogenicity. Recent studies have revealed non-canonical forms of ADP-ribosylation, including modifications of nucleic acids and the discovery of new ADP-ribose derivatives. The physiological relevance of non-canonical ADP-ribosylation deserves recognition alongside traditional protein ADP-ribosylation modification.
BIOCHEMICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Zeeshan Javed, Hannah Nguyen, Kiana Harker, Christian Mohr, Pia Vano, Sean Wallace, Clarissa Silvers, Colin Sim, Soumya Turumella, Ally Flinn, Anthony Moritz, Ian Carter-O'Connell
Summary: This study presents a MALDI-TOF method for analyzing proximal factors that guide ARTD target selection. It reveals that PARP14 enzyme has specific sequence requirements for glutamate/aspartate targeting and neighboring residues play an important role in PARP14 targeting. The study also shows that the enzymatic and non-enzymatic removal of ADPr modification is influenced by pH, temperature, and sequence, and highlights differential activities within the glycohydrolase family and their sequence preferences using ADPr-peptides.
Article
Biotechnology & Applied Microbiology
Chia-Ching Chou, Michael T. Patel, Marc R. Gartenberg
FEMS YEAST RESEARCH
(2015)
Editorial Material
Biology
Yu-Fan Chen, Marc R. Gartenberg
Review
Genetics & Heredity
Marc R. Gartenberg, Jeffrey S. Smith
Article
Biochemistry & Molecular Biology
Yu-Fan Chen, Chia-Ching Chou, Marc R. Gartenberg
MOLECULAR AND CELLULAR BIOLOGY
(2016)
Review
Genetics & Heredity
Marc R. Gartenberg, Jeffrey S. Smith
Article
Multidisciplinary Sciences
Melinda S. Borrie, John S. Campor, Hansa Joshi, Marc R. Gartenberg
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Biochemistry & Molecular Biology
Marc Gartenberg
CHROMOSOME RESEARCH
(2009)
Article
Cell Biology
Marc R. Gartenberg
GENES & DEVELOPMENT
(2009)
Article
Cell Biology
Miao Chen, Marc R. Gartenberg
GENES & DEVELOPMENT
(2014)
Article
Multidisciplinary Sciences
Giulia J. Ruben, Jacob G. Kirkland, Tracy MacDonough, Miao Chen, Rudra N. Dubey, Marc R. Gartenberg, Rohinton T. Kamakaka
Article
Multidisciplinary Sciences
Sookhee Park, Erin E. Patterson, Jenel Cobb, Anjon Audhya, Marc R. Gartenberg, Catherine A. Fox
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2011)
Article
Genetics & Heredity
Ching-Shyi Wu, Yu-Fan Chen, Marc R. Gartenberg
Article
Cell Biology
Catherine A. Fox, Marc R. Gartenberg
Article
Genetics & Heredity
Miao Chen, Qian Long, Melinda S. Borrie, Haohui Sun, Changlin Zhang, Han Yang, Dingbo Shi, Marc R. Gartenberg, Wuguo Deng
Summary: The nuclear pore protein TPR and export factor NXF1 play essential roles in regulating tRNA nuclear export and protein synthesis, with TPR being overexpressed in lung cancer tissues and associated with poor prognosis.
Article
Biochemistry & Molecular Biology
Melinda S. Borrie, Paul M. Kraycer, Marc R. Gartenberg
Summary: Cohesin is an important element of chromosomes that regulates DNA events. It holds sister chromatids together and organizes chromosomal DNAs. This study explores the translocation of cohesin under different conditions in yeast and reveals the limitations to its movement on chromosomes.
MOLECULAR AND CELLULAR BIOLOGY
(2023)