Article
Fisheries
Hongxia Jiang, Yizheng Li, Shuaishuai Zhang, Huanxin Li, Xuewei Liu, Ran Zhang, Meng Zhang, Lei Wang, Miao Yu, Zhigang Qiao, Xuejun Li
Summary: In this study, the function of Cyclooxygenase (COX) in ovarian development and ovulation in Mac-robrachium nipponense was investigated. The complete cDNA of COX in M. nipponense was identified and its sequence and protein structure were analyzed. The results showed that MnCOX plays an important role in regulating ovarian development and ovulation in M. nipponense. Knockdown of MnCOX led to decreased PGE2 content, expression of Vg and Vgr genes, and GSI of prawns, while AA administration increased MnCOX expression and oviposition rate.
AQUACULTURE REPORTS
(2023)
Article
Oncology
Timo-Pekka K. Lehto, Ruusu-Maaria Kovanen, Susanna Lintula, Adrian Malen, Carolin Sturenberg, Andrew Erickson, Olli-Pekka Pulkka, Ulf-Hakan Stenman, Eleftherios P. Diamandis, Antti Rannikko, Tuomas Mirtti, Hannu Koistinen
Summary: This study aimed to investigate the mRNA levels and prognostic impact of all 15 human kallikrein-related peptidases (KLKs) and their targets, proteinase-activated receptors (PARs), in surgically treated prostate cancer (PCa). The results showed that the expression of KLK2, KLK3, KLK4, and KLK15 was closely associated with tumor aggressiveness and prognosis, suggesting their potential as prognostic biomarkers for PCa.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Medicine, General & Internal
Seong Hee Kang, Hyung Joon Yim, Ji-won Hwang, Mi-jung Kim, Young-Sun Lee, Young Kul Jung, Hyungshin Yim, Baek-Hui Kim, Hae-Chul Park, Yeon Seok Seo, Ji Hoon Kim, Jong Eun Yeon, Soon Ho Um, Kwan Soo Byun
Summary: Combination therapy with a statin, simvastatin, and a COX-2 inhibitor, NS-398, has synergistic anti-fibrotic effects through multiple pathways. These findings provide a theoretical basis for the clinical application of this treatment strategy in advanced liver diseases with hepatic fibrosis.
KOREAN JOURNAL OF INTERNAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Jin-Bo Zhang, Zhan-Jun Lu, Hai-Zhong Yu
Summary: This study identifies the role of DcGSK3 in regulating chitin and fatty acid metabolism in the Asian citrus psyllid. Silencing of DcGSK3 through RNA interference greatly reduces the expression levels of genes associated with carbohydrate and fatty acid metabolism in the psyllids, highlighting the significant regulatory function of DcGSK3 in these processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yutong Zhu, Linghao Kong, Xinqian Wang, Jiazheng Xu, Xuhong Qian, Yangyang Yang, Zhiping Xu, Kun Yan Zhu
Summary: By utilizing RNA interference system based on rolling circle transcription technology, the expression of two crucial chitin synthetic pathway genes in oriental armyworm larvae was successfully suppressed, leading to significant effects on larval development. This study provides a new approach for studying gene functions and developing bio-pesticides for insect pest management.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zhenyu Li, Mengling Chen, Wenjie Bai, Shuxia Zhang, Liwei Meng, Wei Dou, Jinjun Wang, Guorui Yuan
Summary: By studying the genome database, 33 BdCarEs genes were identified and classified into nine clades using phylogenetic analysis. BdCarEs genes exhibit diverse potential functions in different physiological processes and can participate in resistance to insecticides with different modes of action. It was found that highly expressed BdCarEs genes were significantly up-regulated in the MR strain, and knockdown of these genes increased the susceptibility of the oriental fruit fly to corresponding insecticides.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Bi-Yun Zhang, Feng-Qi Li, Cheng Qu, Youssef Dewer, Yue-Jun Fu, Chen Luo
Summary: In this study, researchers identified genes related to sex pheromone biosynthesis in the Noctuid moths Spodoptera litura and Spodoptera exigua. They found that only the genes SlitDes5 and SexiDes5 were significantly overexpressed in the sex pheromone glands of these moths. Functional analysis of the SlitDes5 gene showed that its expression level could be reduced by 49.42% through RNA interference. These findings contribute to a better understanding of the key genes involved in sex pheromone biosynthesis and provide potential gene targets for pest control strategies.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Entomology
Gang Li, Xun-Yan Liu, Guy Smagghe, Jin-Zhi Niu, Jin-Jun Wang
Summary: Spider mites have one ecdysone receptor (EcR) and multiple retinoid X receptors (RXRs), but the specific functions of these RXRs in mite development remain unclear. The study found different expression patterns of PcRXR1 and PcRXR2 during different stages, suggesting potential roles in cell autophagy, tissue remodeling, and cuticle secretion.
Article
Biochemistry & Molecular Biology
Ping Zhang, Peiting Ying, Hongzhe Li, Ning Zhao, Rongrong Liu, Sisi Li, Weiqun Xu, Yang Tang, Yongmin Tang
Summary: Knocking down interferon gamma (IFN-gamma) using shRNA was found to be an effective strategy for reducing cytokine storms in anti-CD19 CAR-T cell therapy. The study showed that shIFN-gamma-anti-CD19CAR-T cells can reduce the generation of cytokine storms without compromising therapeutic efficacy. The killing efficacy of shIFN-gamma-anti-CD19CAR-T cells was similar to regular anti-CD19CAR-T cells in both in vitro and mouse model experiments.
Article
Oncology
Peijie Wu, Xiuqing Yang, Ling Qiao, Yanju Gong
Summary: This study identified six mRNA signatures that showed the ability to detect CRC and pan-cancer samples, serving as potential diagnostic markers.
JOURNAL OF ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Julia Korzuch, Monika Rak, Katarzyna Balin, Maciej Zubko, Olga Glowacka, Mateusz Dulski, Robert Musiol, Zbigniew Madeja, Maciej Serda
Summary: This paper presents two water-soluble fullerene nanomaterials developed for non-viral siRNA transfection. One fullerene is an efficient siRNA transfection agent, while the other is inactive, likely due to the formation of an extremely stable complex with siRNA.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Sabrina Borchert, Thomas Herold, Stavros Kalbourtzis, Rainer Hamacher, Yvonne Krause, Sophia Berger, Wiebke K. Guder, Arne Streitbuerger, Jendrik Hardes, Moritz Goetz, Sebastian Bauer, Hans-Ulrich Schildhaus
Summary: This study aimed to validate and test the feasibility, reliability, and technical applicability of a new commercially available transcriptome profiling assay on FFPE patient samples. The results showed that the assay is a feasible method for adequate transcriptome profiling with low sample input, making it suitable for biomarker research.
Article
Biochemical Research Methods
Rajendra Kumar Gurumurthy, Klaus-Peter Pleissner, Cindrilla Chumduri, Thomas F. Meyer, Andre P. Maeurer
Summary: SOPRA is a novel analysis workflow that utilizes regression analysis of non-averaged object features from cell populations to generate control-normalized frequency distribution profiles for the detection of significantly altered cell populations. By integrating maSigPro into the workflow, statistically significant frequency distributions can be identified, allowing for the detection of siRNAs and compounds with similar phenotypes.
BMC BIOINFORMATICS
(2022)
Article
Biochemistry & Molecular Biology
Chao Yang, Dejun Ma, Liqing Lu, Xing Yang, Zhen Xi
Summary: This study successfully synthesized KUE-siRNA conjugates targeting PSMA, which were able to selectively enter and down-regulate STAT3 expression in prostate cancer cells, providing a potential new method for cell-targeted gene silencing.
Article
Pharmacology & Pharmacy
Yao Sun, Michael F. Cronin, Monique C. P. Mendonca, Jianfeng Guo, Caitriona M. O'Driscoll
Summary: Prostate cancer is a serious disease that poses a threat to men's health, and traditional treatments face challenges such as poor prognosis and tumor resistance. A sialic acid-targeted nanoparticle is developed to deliver specific siRNA to tumor-associated macrophages, inhibiting the growth and metastasis of prostate cancer cells.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)