Letter
Urology & Nephrology
Brandon Renner, Felix Poppelaars, Jennifer Laskowski, Natalie J. Serkova, Liudmila Kulik, V. Michael Holers, Joshua M. Thurman
Summary: Histologic quantification of complement C3 deposits in kidney biopsies provides prognostic information in patients with glomerulonephritis. Unfortunately, kidney biopsies are invasive procedures and limited in their ability to capture the whole picture. The researchers have developed a noninvasive imaging method using a bioluminescent resonance energy transfer construct to detect specific C3 fragment deposition in both kidneys, providing a potential tool for monitoring kidney inflammation.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Article
Engineering, Environmental
Pak Wai Wong, Vicki Man-Wai Yim, Jiaxin Guo, Ben Sun Chan, Bhaskar Jyoti Deka, Alicia Kyoungjin An
Summary: Membrane distillation (MD) is a promising technology for treating high salinity wastewater, but wetting due to surfactants can impede its application. Impedance spectroscopy has been proposed for early wetting detection, providing a more detailed insight into the wetting phenomenon.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Xingya Jiang, Qinhan Zhou, Bujie Du, Siqing Li, Yingyu Huang, Zhikai Chi, William M. Lee, Mengxiao Yu, Jie Zheng
Summary: This study demonstrates a new method for non-invasively and consecutively monitoring hepatic glutathione depletion using in vivo fluorescence imaging and blood testing with the assistance of a body-clearable nanoprobe, potentially opening up new avenues for clinical disease detection.
Review
Gastroenterology & Hepatology
Azarakhsh Baghdadi, Sahar Mirpour, Maryam Ghadimi, Mina Motaghi, Bita Hazhirkarzar, Timothy M. Pawlik, Ihab R. Kamel
Summary: Colorectal cancer metastasis to the liver is common and often treated with surgical resection, although not all patients are eligible. Imaging plays a critical role in evaluating the characteristics and treatment of CRLM, with CT being the modality of choice for diagnosis and management.
JOURNAL OF GASTROINTESTINAL SURGERY
(2022)
Review
Oncology
Drew Maclean, Maria Tsakok, Fergus Gleeson, David J. Breen, Robert Goldin, John Primrose, Adrian Harris, James Franklin
Summary: Colorectal liver metastases exhibit heterogenous histopathological and immunohistochemical phenotypes, impacting treatment responses and outcomes. Advanced imaging techniques like multiparametric MRI and functional imaging have the potential to provide clinically-actionable phenotypic characterisation. Advanced analysis techniques such as radiomics and machine learning play a growing role in linking imaging features to clinically relevant tumour phenotypes.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Dingsheng Liu, Chunsheng Chen, Mingming Cui, Hong Zhang
Summary: Recent studies have identified miR-140-3p as a tumor suppressor in colorectal cancer (CRC) progression by targeting BCL9 and BCL2, inhibiting cell proliferation, migration, invasion, and promoting apoptosis. This suggests that the miR-140-3p-BCL9/BCL2 axis may be a potential target for miRNA-based therapy and prognostication of CRC.
Review
Chemistry, Multidisciplinary
Xiaohan Liu, Limeng Pan, Kaiye Wang, Wei Pan, Na Li, Bo Tang
Summary: This review summarizes and discusses the importance of real-time monitoring of the immune response to evaluate immune status and guide immunotherapy. It also explores various imaging methods, immune-related imaging probes, and current challenges and opportunities.
Article
Biochemistry & Molecular Biology
Eirini Martinou, Carla Moller-Levet, Dimitrios Karamanis, Izhar Bagwan, Angeliki M. Angelidi
Summary: HOXB9 plays an important role in colorectal cancer, promoting disease progression and affecting overall survival. In vitro experiments showed that HOXB9 overexpression increased cell proliferation and upregulated the expression of genes related to epithelial-mesenchymal transition. In patients with colorectal liver metastases, high expression of HOXB9 was associated with worse overall survival.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Piero Boraschi, Francescamaria Donati, Rosa Cervelli, Federica Pacciardi, Gaia Tarantini, Maura Castagna, Lucio Urbani, Riccardo Lencioni
Summary: The preADC value can be predictive of the lesion ChT response; PostADC and Delta ADC values are important for evaluating tumor treatment response after preoperative ChT in patients with colorectal liver metastases; There is a significant correlation between TRG and RECIST1.1 criteria in assessing the chemoresponse of liver metastases.
EUROPEAN JOURNAL OF RADIOLOGY
(2021)
Review
Medicine, General & Internal
Sajida Maryam, Katarzyna Krukiewicz, Ihtisham Ul Haq, Awal Ayaz Khan, Galal Yahya, Simona Cavalu
Summary: Cancer is a leading cause of death in developed and developing countries, with colorectal cancer being the third most common cause worldwide. Risk factors for colorectal cancer include obesity, a poor diet, physical inactivity, and smoking. New diagnostic and prognostic biomarkers are needed to improve outcomes.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Engineering, Electrical & Electronic
Jie Yuan, Hailin Wang, Peng Wu, Chuanjun Wang, Jinming Chen, Hao Jiao
Summary: This article introduces a new method for extracting features from electrical signal waveforms, which segments the waveform and extracts shape, harmonic, and statistic features to enhance precision and recall in noninvasive load monitoring. The experimental results show significant improvement in identifying states with a finer granularity compared to state-of-the-art methods.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2021)
Article
Oncology
Anastasios D. Giannou, Jan Kempski, Tao Zhang, Joeran Luecke, Ahmad Mustafa Shiri, Dimitra E. Zazara, Ioannis Belios, Andres Machicote, Philipp Seeger, Theodora Agalioti, Joseph Tintelnot, Adrian Sagebiel, Miriam Tomczak, Lennart Bauditz, Tanja Bedke, Lorenz Kocheise, Baris Mercanoglu, Mohammad Fard-Aghaie, Emmanouil Giorgakis, Panagis M. Lykoudis, Anastasia Pikouli, Julia-Kristin Grass, Ramez Wahib, Jan Bardenhagen, Benjamin Brunswig, Asmus Heumann, Tarik Ghadban, Anna Dupree, Michael Tachezy, Nathaniel Melling, Petra C. Arck, Pablo Stringa, Maria Virginia Gentilini, Gabriel E. Gondolesi, Ryosuke Nakano, Angus W. Thomson, Daniel Perez, Jun Li, Oliver Mann, Jakob R. Izbicki, Nicola Gagliani, Ioannis C. Maroulis, Samuel Huber
Summary: This study found that the progression of colorectal cancer is associated with tumor metastasis. In vivo models using mice showed that injection of cancer cells into the colon or spleen resulted in lung and liver metastasis. The expression level of IL22BP in colorectal cancer patients was correlated with metastatic tumor stages. Therefore, IL-22 might be a potential therapeutic target against the progression of metastatic colorectal cancer.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Hai-Bin Zhu, Da Xu, Meng Ye, Li Sun, Xiao-Yan Zhang, Xiao-Ting Li, Pei Nie, Bao-Cai Xing, Ying-Shi Sun
Summary: The study established and validated a deep learning algorithm using prechemotherapy and postchemotherapy MRI to predict pathological response in patients with CRLM. The MRI-based model showed accurate prediction of response and survival outcomes after hepatectomy, providing potential for individualized treatment.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Review
Gastroenterology & Hepatology
Jung Hwan Yu, Han Ah Lee, Seung Up Kim
Summary: Nonalcoholic fatty liver disease (NAFLD) is a common and serious liver disorder, with liver fibrosis being a key factor in prognosis. However, traditional liver biopsy is invasive and costly, leading to the development of noninvasive imaging biomarkers such as elastography and magnetic resonance imaging. Additionally, artificial intelligence and deep learning techniques are being utilized to improve the accuracy of these imaging methods. In this review, we discuss the usefulness and future prospects of noninvasive imaging biomarkers in evaluating liver fibrosis in patients with NAFLD.
CLINICAL AND MOLECULAR HEPATOLOGY
(2023)
Article
Oncology
Wei-Jie Song, Fan Zhang, Zhi-Yong Wang, Zhao-Song Wang, Bi-Yun Wang, Jun-Rong Jia
Summary: In this study, a successful mouse model of CRC metastasis for clinical application has been established, and the effect of 5-FU treatment has been evaluated. Micro-CT and in vivo fluorescence imaging can effectively monitor the progression of metastatic CRC as non-invasive methods. 5-FU treatment can reduce the viability of metastatic CRC cells and prolong the survival of patients.
TRANSLATIONAL CANCER RESEARCH
(2023)
Article
Virology
Beatriz Pacin-Ruiz, Maria Francesca Cortese, David Tabernero, Sara Sopena, Josep Gregori, Selene Garcia-Garcia, Rosario Casillas, Adrian Najarro, Unai Aldama, Adriana Palom, Ariadna Rando-Segura, Anna Galan, Marta Vila, Mar Riveiro-Barciela, Josep Quer, Gloria Gonzalez-Aseguinolaza, Maria Buti, Francisco Rodriguez-Frias
Summary: This study used next-generation sequencing to analyze the ribozyme quasispecies of hepatitis delta virus (HDV). The ribozyme was found to be highly conserved overall, with a hyper-conserved region between nucleotides 715-745. Several mutations were observed, with the deletion of C64 showing a strong impact on HDV replication. The findings suggest that the conserved region of the ribozyme could be a potential target for gene-silencing strategies.
Article
Oncology
Borja Ruiz-Fernandez de Cordoba, Haritz Moreno, Karmele Valencia, Naiara Perurena, Pablo Ruedas, Thomas Walle, Alberto Pezonaga-Torres, Juan Hinojosa, Elisabet Guruceaga, Antonio Pineda-Lucena, Marta Abengozar-Muela, Denis Cochonneau, Carolina Zandueta, Susana Martinez-Canarias, Alvaro Teijeira, Daniel Ajona, Sergio Ortiz-Espinosa, Xabier Morales, Carlos Ortiz de Solorzano, Marta Santisteban, Luis Ramos-Garcia, Laura Guembe, Vratislav Strnad, Dominique Heymann, Sandra Hervas-Stubbs, Ruben Pio, Maria E. Rodriguez-Ruiz, Carlos E. de Andrea, Silvestre Vicent, Ignacio Melero, Fernando Lecanda, Rafael Martinez-Monge
Summary: Locoregional failure in breast cancer patients after surgery and irradiation is linked to a poor prognosis. The ENPP1/HP axis is exploited by tumor cells to promote local recurrence by suppressing immune cells. Blocking this axis offers new opportunities for therapeutic intervention.
Article
Biochemistry & Molecular Biology
Lara Gil-Melgosa, Jorge Grasa, Ainhoa Urbiola, Rafael Llombart, Miguel Susaeta Ruiz, Veronica Montiel, Cristina Ederra, Begona Calvo, Mikel Ariz, Purificacion Ripalda-Cemborain, Felipe Prosper, Carlos Ortiz-de-Solorzano, Juan Pons-Villanueva, Ana Perez Ruiz
Summary: This study investigates the morphological changes in the tendon, the myotendinous junction, and muscle belly after Achilles tendon rupture in a mouse model. The findings reveal cellular and fatty infiltration, fibrotic tissue accumulation, muscle stem cell decline, and collagen fiber disorganization, which contribute to the loss of muscle mechanical function. This research is important for improving regenerative medicine practices for Achilles tendon ruptures.
Article
Oncology
Sergio Ortiz-Espinosa, Xabier Morales, Yaiza Senent, Diego Alignani, Beatriz Tavira, Irati Macaya, Borja Ruiz, Haritz Moreno, Ana Remirez, Cristina Sainz, Alejandro Rodriguez-Pena, Alvaro Oyarbide, Mikel Ariz, Maria P. Andueza, Karmele Valencia, Alvaro Teijeira, Kai Hoehlig, Axel Vater, Barbara Rolfe, Trent M. Woodruff, Jose Maria Lopez-Picazo, Silvestre Vicent, Grazyna Kochan, David Escors, Ignacio Gil-Bazo, Jose Luis Perez-Gracia, Luis M. Montuenga, John D. Lambris, Carlos Ortiz de Solorzano, Fernando Lecanda, Daniel Ajona, Ruben Pio
Summary: This study reveals that C5a can enhance the capacity of PMN-MDSCs to promote tumor growth and metastasis by inducing the formation of NETs. The formation of NETs is dependent on the production of HMGB1 by cancer cells. Inhibiting C5a, C5aR1, or NETosis can reduce the number of circulating tumor cells and the metastatic burden in a mouse lung metastasis model. The translational relevance of these findings is supported by the stimulation of migration and NETosis in PMN-MDSCs obtained from lung cancer patients.
Article
Computer Science, Artificial Intelligence
Daniel Jimenez-Sanchez, Mikel Ariz, Hang Chang, Xavier Matias-Guiu, Carlos E. de Andrea, Carlos Ortiz-de-Solorzano
Summary: NaroNet is a deep learning framework that models the multi-scale tumor microenvironment from multiplex-stained cancer tissue images and provides patient-level interpretable predictions. The model learns the influence of cell phenotypes, cell neighborhoods, and neighborhood interactions on predicting the correct label through unsupervised learning.
MEDICAL IMAGE ANALYSIS
(2022)
Article
Biochemical Research Methods
Manuela Gonzalez-Aparicio, Maria Bunuales, Inaki Ortiz de Landazuri, Jesus Prieto, Ruben Hernandez-Alcoceba
Summary: This study improves the performance of packaging cells and increases the production efficiency of high-capacity adenoviral vectors (HC-AdV) by using a dimerizable Cre system. It also reduces the time and cost of HC-AdV production, contributing to the standardization of HC-AdV production.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Andrea Bazo, Aquilino Lantero, Itsaso Mauleon, Leire Neri, Martin Poms, Johannes Haberle, Ana Ricobaraza, Bernard Benichou, Jean-Philippe Combal, Gloria Gonzalez-Aseguinolaza, Rafael Aldabe
Summary: This study evaluated the therapeutic efficacy of gene therapy for treating Citrullinemia type I (CTLN1). The results showed that this treatment method can improve the condition and increase the survival rate of patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Gastroenterology & Hepatology
Nerea Zabaleta, Carmen Unzu, Nicholas D. Weber, Gloria Gonzalez-Aseguinolaza
Summary: In this review, the authors discuss gene therapy involving the use of recombinant adeno-associated virus vectors for the treatment of inherited liver diseases, including ongoing clinical trials that are producing promising results. Clinical testing has revealed several key challenges associated with gene therapy, such as the interaction of the immune system with the vector and the risk of adverse events. The permanent modification of the host genome through gene editing raises concerns about oncogenicity and requires careful evaluation.
NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY
(2023)
Article
Gastroenterology & Hepatology
Nicholas D. Weber, Leticia Odriozola, Irene Ros-Ganan, Guillermo Garcia-Porrero, David Salas, Josepmaria Argemi, Jean-Philippe Combal, Takashi K. Kishimoto, Gloria Gonzalez-Aseguinolaza
Summary: The study demonstrates that repeat administration of rAAV combined with immunotolerant nanoparticles can effectively treat PFIC3 in infant mice and reduce the risk of liver cancer.
Review
Biotechnology & Applied Microbiology
Helena Costa-Verdera, Carmen Unzu, Erika Valeri, Sahil Adriouch, Gloria Gonzalez Aseguinolaza, Federico Mingozzi, Anna Kajaste-Rudnitski
Summary: As the clinical experience in AAV vector-based gene therapies grows, there is a need to better understand and control the host immune responses. Our current understanding of the immune responses to AAV gene therapies is limited, which is further hindered by the lack of replication of clinical findings in preclinical animal models.
HUMAN GENE THERAPY
(2023)
Article
Computer Science, Interdisciplinary Applications
Daniel Jimenez-Sanchez, Mikel Ariz, Carlos E. De Andrea, Carlos Ortiz-De-Solorzano
Summary: Multiplex immunofluorescence is a highly relevant imaging technique for studying the tumor microenvironment and discovering disease biomarkers. The analysis of these images requires the use of machine learning tools and the availability of large image datasets. This study presents Synplex, a computer simulator that generates multiplexed immunofluorescence images based on user-defined parameters.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2023)
Article
Cell Biology
Susana Aguilar, Paula Garcia-Olloqui, Lidia Amigo-Moran, Jose Luis Toran, Juan Antonio Lopez, Guillermo Albericio, Gloria Abizanda, Diego Herrero, Africa Vales, Saray Rodriguez-Diaz, Marina Higuera, Ruben Garcia-Martin, Jesus Vazquez, Carmen Mora, Gloria Gonzalez-Aseguinolaza, Felipe Prosper, Beatriz Pelacho, Antonio Bernad
Summary: Oxidative stress-induced myocardial apoptosis and necrosis play a critical role in ischemic infarction. This study identified and validated miR-935 as a highly differentially expressed miRNA in exosomes derived from human cardiac progenitor cells (CPC). Reduction of miR-935 expression promotes oxidative stress-associated apoptosis. Together with other exosomal miRNA members, miR-935 may counteract oxidative stress-related apoptosis in the CPC microenvironment.
Article
Pharmacology & Pharmacy
Andoni Gomez-Moreno, Enara San Sebastian, Jennifer Moya, Pilar Gomollon-Zueco, Sergio Isola, Africa Vales, Gloria Gonzalez-Aseguinolaza, Carmen Unzu, Urtzi Garaigorta
Summary: Gene therapy is a promising approach to treat inherited metabolic liver disorders. This study shows that using topoisomerase inhibitors can increase the expression of viral genes in non-dividing hepatic cells, with a greater effect seen in the integration-deficient LV strain. These effects are associated with increased DNA damage response.
Article
Biochemistry & Molecular Biology
Kharmen Billimoria, Yuri A. Diaz Fernandez, Elina Andresen, Ioritz Sorzabal-Bellido, Gonzalo Huelga-Suarez, Dorota Bartczak, Carlos Ortiz de Solorzano, Ute Resch-Genger, Heidi Goenaga Infante
Summary: This paper discusses the feasibility of a novel strategy based on the combination of bioprinting nano-doping technology and laser ablation-inductively coupled plasma time-of-flight mass spectrometry analysis for the preparation and characterization of gelatin-based multi-element calibration standards suitable for quantitative imaging. The results show that bioprinting method has better between batch repeatability and elemental signal homogeneity compared to traditional cryosectioning method. Additionally, the bioprinting method can significantly reduce the preparation time of standards and the bio-printed calibration standards are stable for a long period of time.
Article
Immunology
Irene Ros-Ganan, Mirja Hommel, Laia Trigueros-Motos, Blanche Tamarit, Estefania Rodriguez-Garcia, David Salas, Guiomar Perez, Anne Douar, Jean Philippe Combal, Bernard Benichou, Veronica Ferrer, Gloria Gonzalez-Aseguinolaza
Summary: This study tested the effectiveness of an enzyme called IdeS in cleaving neutralizing antibodies and improving the transduction efficiency of AAV-mediated gene therapy. The results showed that IdeS was able to efficiently cleave human IgG and had varying effects on different AAV serotypes in vivo. The study suggests that preconditioning with IdeS could provide a unique treatment opportunity for patients who are excluded from gene therapy due to elevated levels of anti-AAV antibodies.
CLINICAL & TRANSLATIONAL IMMUNOLOGY
(2022)
