Article
Pediatrics
Nicola Pini, Zihe Huo, Urs Kym, Stefan Holland-Cunz, Stephanie J. Gros
Summary: Neuroblastoma is a highly heterogeneous tumor with various adverse factors linked to its oncogenesis, progression, and metastases. The expression of AQP1 in neuroblastoma correlates with changing cellular factors, particularly hypoxic status, NMYC and NCAM expression. Additionally, AQP1 expression is associated with increased migratory behavior of neuroblastoma cells under hypoxic conditions.
Article
Oncology
Ellen M. Westerhout, Mohamed Hamdi, Peter Stroeken, Natalia E. Nowakowska, Arjan Lakeman, Jennemiek van Arkel, Nancy E. Hasselt, Boris Bleijlevens, Nurdan Akogul, Franciska Haneveld, Alvin Chan, Peter van Sluis, Danny Zwijnenburg, Richard Volckmann, Carel J. M. van Noesel, Igor Adameyko, Tim van Groningen, Jan Koster, Linda J. Valentijn, Johan van Nes, Rogier Versteeg
Summary: Neuroblastoma cells can adopt different states, and immature MES cells have shown resistance to chemotherapy. However, dual treatment with ALK inhibitors and MES cell-targeting TRAIL can delay tumor relapse.
Article
Cell Biology
Huijuan Zeng, Jing Pan, Chao Hu, Jiliang Yang, Jiahao Li, Tianbao Tan, Manna Zheng, Yuanchao Shen, Tianyou Yang, Yun Deng, Yan Zou
Summary: The study reveals that in neuroblastoma, the overexpression of SNHG25 promotes NB cell proliferation, invasion, and migration, while also regulating the expression of SNORA50C and the assembly of associated snoRNP. Furthermore, SNHG25 inhibits the ubiquitination of HDAC1 through SNORA50C, thereby increasing HDAC1 expression and promoting NB cell growth and migration via the HDAC1-mediated pathway. In vivo experiments validate that SNHG25 promotes NB progression through the SNORA50C/HDAC1 pathway.
CELL DEATH & DISEASE
(2022)
Editorial Material
Oncology
Hedwig E. Deubzer, Kathy Astrahantseff, Marco Lodrini
Summary: In this paper, the authors demonstrate the important role of circulating cell-free tumor DNA sequencing in detecting the genomic evolution of neuroblastoma under ALK inhibitor therapy and identifying novel (sub)clonal pathogenic variants involved in disease progression under conventional therapy. This highlights the significance of circulating cell-free tumor DNA sequencing in revealing intratumor heterogeneity and tumor evolution.
Article
Oncology
Laura Garcia-Gerique, Marta Garcia, Alicia Garrido-Garcia, Soledad Gomez-Gonzalez, Montserrat Torrebadell, Estela Prada, Guillem Pascual-Pasto, Oscar Munoz, Sara Perez-Jaume, Isadora Lemos, Noelia Salvador, Monica Vila-Ubach, Ana Doncel-Requena, Mariona Sunol, Angel M. Carcaboso, Jaume Mora, Cinzia Lavarino
Summary: This study identifies macrophage migration inhibitory factor (MIF) as a key cytokine involved in the infiltration of neuroblastoma (NB) cells in the bone marrow (BM). In the BM microenvironment, NB cells produce MIF, which is associated with enhanced invasion and survival of NB cells, as well as activation of the PI3K/AKT and MAPK/ERK signaling pathways. Pharmacological inhibition of MIF demonstrates the anti-tumor efficacy of targeting MIF, inhibiting NB aggressiveness, improving drug response, delaying NB growth, and improving survival in the xenograft model.
Article
Multidisciplinary Sciences
Carl W. White, Laura E. Kilpatrick, Kevin D. G. Pfleger, Stephen J. Hill
Summary: The study demonstrates a sensitive real-time approach to quantify secretion and receptor binding of native chemokines in live cells for better understanding of their molecular interactions and function. By using CRISPR/Cas9 genome editing to tag chemokine CXCL12 with nanoluciferase fragment HiBiT, the secretion and binding of chemokines can be monitored and quantified. These live cell approaches combine the sensitivity of nanoluciferase with CRISPR/Cas9 genome editing to detect, quantify, and monitor binding of low levels of native secreted proteins in real time.
Article
Medicine, Research & Experimental
Thale Kristin Olsen, Cecilia Dyberg, Bethel Tesfai Embaie, Adele Alchahin, Jelena Milosevic, Jane Ding, Jorg Otte, Conny Tummler, Ida Hed Myrberg, Ellen M. Westerhout, Jan Koster, Rogier Versteeg, Han-Fei Ding, Per Kogner, John Inge Johnsen, David B. Sykes, Ninib Baryawno
Summary: By applying a multiomic system approach, researchers identified DHODH as a potential treatment target for high-risk neuroblastoma. Inhibiting DHODH could significantly reduce tumor growth and improve survival rates. Combining the DHODH inhibitor brequinar with temozolomide showed promising therapeutic potential in neuroblastoma.
Article
Multidisciplinary Sciences
Hai-Feng Zhang, Alberto Delaidelli, Sumreen Javed, Busra Turgu, Taylor Morrison, Christopher S. Hughes, Xiaqiu Yang, Manideep Pachva, Michael M. Lizardo, Gurdeep Singh, Jennifer Hoffmann, Yue Zhou Huang, Khushbu Patel, Rawan Shraim, Sonia H. Y. Kung, Gregg B. Morin, Samuel Aparicio, Daniel Martinez, John M. Maris, Kristopher R. Bosse, Karla C. Williams, Poul H. Sorensen
Summary: This study reveals that GREB1 gene, located near MYCN locus, is frequently coexpressed with MYCN and promotes cell survival in high-risk neuroblastoma (NB). The research also identifies MYO1B gene as a crucial regulator of invasion and metastasis in MYCN amplified NB. Furthermore, MYO1B is found to regulate secretome reprogramming and the cytokine MIF mediates its pro-invasive and pro-metastatic activity.
Article
Multidisciplinary Sciences
Hengzhong Guo, Yangyang Zhu, Chaozhi Li, Ya Wang, Gejing De, Lili Lu
Summary: In this study, it was found that EV71 induces GSDMD/NLRP3-mediated pyroptosis in SH-SY5Y cells through up-regulated miR-146a. The potential target of miR-146a was identified as CXCR4. It was observed that CXCR4 expression was regulated by miR-146a during EV71 infection, and over-expression of CXCR4 attenuated EV71-induced pyroptosis in SY-SY5Y cells. These findings reveal a previously unrecognized mechanism in which EV71 induces nervous system cell damage through regulation of miR-146a/CXCR4-mediated pyroptosis.
Article
Cell Biology
Xiaofei Wang, Ran Zhou, Yanzhen Xiong, Lingling Zhou, Xiang Yan, Manli Wang, Fan Li, Chuanxing Xie, Yiming Zhang, Zongyao Huang, Chaoqiong Ding, Kaidou Shi, Weida Li, Yu Liu, Zhongwei Cao, Zhen-Ning Zhang, Shengtao Zhou, Chong Chen, Yan Zhang, Lu Chen, Yuan Wang
Summary: The study established two highly invasive models of malignant glioma derived from human neural stem/progenitor cells, revealing the process of gliomagenesis from neural stem cells to glioblastoma and identifying potential early treatment options.
Article
Immunology
Luming Zhao, Shaomin Hu, Micha L. Davila, Jie Yang, Yang-Ding Lin, Joseph M. Albanese, Yungtai Lo, Yanhua Wang, Mary J. Kennett, Qiang Liu, Na Xiong
Summary: The gut-homing chemokine receptor CCR10(+)IgA-ASCs form conjugates with helper T cells, preferentially regulatory T cells, at their differentiation sites of gut-associated lymphoid organs for their coordinated co-localization into the colon to promote local homeostasis.
MUCOSAL IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Xi Lu, Naga Prathyusha Maturi, Malin Jarvius, Irem Yildirim, Yonglong Dang, Linxuan Zhao, Yuan Xie, E-Jean Tan, Pengwei Xing, Rolf Larsson, Marten Fryknas, Lene Uhrbom, Xingqi Chen
Summary: By comparing the chromatin accessibility landscape of glioblastoma stem cell (GSC) cultures from mice and patients, the authors suggest that the epigenome of GSCs is regulated by cell lineage and could predict patient survival.
NATURE COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Jinlin Zhang, Mingyue Wang, Doudou He, Liang Zhang, Tianqing Liu, Kaikai Wang
Summary: This study reports a novel delivery system that enhances immune response by targeting PD-L1 and alleviates T cell exhaustion by inhibiting TOX expression. The system demonstrates excellent anti-tumor and antimetastatic effects, providing a potential strategy for treating cold tumors.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
L. E. Wadkin, S. Orozco-Fuentes, I. Neganova, M. Lako, N. G. Parker, A. Shukurov
Summary: This study utilizes experimental data and mathematical modeling to explore the pluripotency regulation mechanisms of human pluripotent stem cells, presenting two mathematical models and evaluating their effectiveness. The research findings suggest that these models can adequately describe the changes in OCT4 expression levels during cell differentiation.
Article
Hematology
Marta Biondi, Sarah Tettamanti, Stefania Galimberti, Beatrice Cerina, Chiara Tomasoni, Rocco Piazza, Samantha Donsante, Simone Bido, Vincenzo Maria Perriello, Vania Broccoli, Andrea Doni, Francesco Dazzi, Alberto Mantovani, Gianpietro Dotti, Andrea Biondi, Alice Pievani, Marta Serafini
Summary: Acute myeloid leukemia (AML) is a hematological malignancy characterized by abnormal clonal proliferation and differentiation. The efficacy of chimeric antigen receptor (CAR)-T-cell therapy in AML has been hindered by factors such as poor accumulation in the leukemia bone marrow niche. Overexpression of CXCR4 in CAR-T cells may improve T-cell homing to the bone marrow and enhance their contact with AML cells, potentially increasing their therapeutic potential. In conclusion, arming CAR-T cells with CXCR4 represents a promising strategy for AML treatment.