Article
Multidisciplinary Sciences
Haichao Jiang, Qing Lu, Shuqing Qiu, Huihui Yu, Zhengji Wang, Zhichao Yu, Yunrui Lu, Lei Wang, Fan Xia, Yuying Wu, Fan Li, Qinglu Zhang, Gang Liu, Dingding Song, Chonglie Ma, Qi Ding, Xiaobo Zhang, Lin Zhang, Xuetang Zhang, Xu Li, Jianwei Zhang, Jinghua Xiao, Xianghua Li, Naiyuan Wang, Yidan Ouyang, Fasong Zhou, Qifa Zhang
Summary: In this study, researchers characterized a type of CMS called Fujian Abortive cytoplasmic male sterility (CMS-FA) in rice. They identified a nuclear restorer gene, OsRf19, which completely restores hybrid fertility by cleaving the FA182 transcripts in the mitochondrial genome. Comparative sequence analysis revealed that OsRf19 originated from a recent duplication in wild rice relatives. The researchers developed six restorer lines by introgressing OsRf19 into parental lines of elite CMS-WA hybrids, resulting in hybrids with equivalent or better agronomic performance. This study demonstrates the promising potential of CMS-FA/OsRf19 for future hybrid rice breeding.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Yanyan Sun, Yan Zhang, Shungeng Jia, Chunjing Lin, Jingyong Zhang, Hao Yan, Bao Peng, Limei Zhao, Wei Zhang, Chunbao Zhang
Summary: This study performed fine mapping of the Rf3 gene using segregating populations derived from the cross between a restorer line and a CMS line in soybean. The candidate gene Glyma.09G171200, encoding a mitochondria-targeted pentatricopeptide repeat protein, was proposed as the potential candidate for Rf3. A CAPS marker, CAPS1712, co-segregated with Rf3 based on single-nucleotide polymorphisms in Glyma.09G171200. These results are fundamental for the assisted selection and creation of novel restorer lines in soybean.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Yongming Liu, Ling Zhang, Xiaowei Liu, Peng Zhang, Zhuofan Zhao, Hongyang Yi, Moju Cao
Summary: In this study, we identified multiple Rf4-restorer lines for maize CMS-C and developed a molecular marker for Rf4 through molecular marker and genetic analysis. These findings are of great significance for improving the efficiency and quality of maize hybrid seed production.
Article
Horticulture
Yongfu Wang, Min Wei, Panpan Duan, Yan Ma, Tao Zhang, Gaoyuan Zhang, Bingqiang Wei
Summary: In this study, 11 SNPs were used to develop KASP molecular markers related to the Rf gene. KS23 was found to be associated with the Rf gene, with the A/A genotype for restoration and the G/G genotype for maintenance. The genotyping results for KS23 showed a consistency of 91.6% in F2 individuals, with an accuracy of 100% among six restorer lines and 67.8% among 44 inbred lines. These markers will be beneficial for the breeding of restorer lines and the application of the CMS/Rf system in pepper seed production.
SCIENTIA HORTICULTURAE
(2023)
Review
Biochemistry & Molecular Biology
Wenjing Ren, Jinchao Si, Li Chen, Zhiyuan Fang, Mu Zhuang, Honghao Lv, Yong Wang, Jialei Ji, Hailong Yu, Yangyong Zhang
Summary: Hybrid production using Ogura CMS has become an important approach to harness heterosis in vegetables. This article provides an overview of the mapping, cloning, mechanism and application of Ogura CMS and fertility restorer genes in Brassica napus, Brassica rapa, Brassica oleracea and other cruciferous crops, and discusses the current challenges and future research directions in the application of Ogura CMS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Chuande Wang, Lina Lezhneva, Nadege Arnal, Martine Quadrado, Hakim Mireau
Summary: In the study, it was found that PPR-B can revert male sterility in the Ogura CMS system in rapeseed through specific translation inhibition of the mitochondria-encoded CMS causing mRNA orf138. This is the first recognized fertility restorer shown to act in this way.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Qian Yang, Xiaoyi Nong, Jize Xu, Fan Huang, Fang Wang, Jiangsheng Wu, Chunyu Zhang, Chao Liu
Summary: The CMS/Rf system is important for rapeseed production, and this study reveals the mechanisms underlying fertility restoration through morphological observation, gene mapping, and transcriptomic analysis, identifying potential key genes.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Zhixing Nie, Yunpeng Song, Hong Wang, Jianying Chen, Qingliang Niu, Weimin Zhu
Summary: In this study, the Rf gene in pepper CMS lines was mapped and the most likely candidate gene was proposed based on genetic analysis and genomic sequencing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Horticulture
Aatjima Na Jinda, Maneechat Nikornpun, Nakarin Jeeatid, Siwaporn Thumdee, Kamon Thippachote, Tonapha Pusadee, Jutamas Kumchai
Summary: This study aimed to produce a new maternal line and its maintainer line by transferring genes from another variety to restorer lines, facilitating hybrid seed production. The male sterility gene was successfully identified using molecular marker-assisted selection and pollen viability test. The results confirmed the accuracy of using these markers in the early generation stage of a conventional breeding program.
Article
Plant Sciences
Petra Schuhmann, Carina Engstler, Kai Kloepfer, Irene L. Guegel, Amine Abbadi, Felix Dreyer, Gunhild Leckband, Bettina Boelter, Franz Hagn, Juergen Soll, Chris Carrie
Summary: The Tic40 gene has been identified as the restorer gene for the MSL system, and heat treatment is hypothesized to restore fertility by inducing a developmental slow down in the cell cycle. The results of this study have important implications for breeding thermotolerant fertile plants using the MSL system.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Agronomy
Honggen Zhang, Xixu Li, Zuopeng Xu, Xiangqiang Zhao, Zihao Wan, Xiaojun Cheng, Qiaoquan Liu, Minghong Gu, Shuzhu Tang
Summary: In this study, the genetic mechanism of fertility restoration in WA-type CMS in japonica rice was investigated. It was found that a dominant gene Rf4 controls fertility restoration, with preferential fertilization of Rf4 pollen. The Rf4 gene has a minor effect on fertility restoration in WA-type japonica CMS lines, and the dosage effects of Rf genes influence fertility restoration.
Article
Agronomy
Fei Li, Yuhang Zhang, Ying Wang, Heqiong Gao, Nansheng Zhuang
Summary: Research on a new male-sterile clone of rubber tree revealed distinct morphological and cytological characteristics in the flowers, as well as three differentially expressed genes in the mitochondrial transcriptome, with orf126 and orf51 being unique insertions. These findings suggest that orf126 and orf51 may serve as candidate male sterility genes in rubber tree.
Article
Agronomy
Ayumu Takatsuka, Tomohiko Kazama, Kinya Toriyama
Summary: The study obtained a Tadukan-type CMS line and a restorer line through backcrossing, determined the whole-genome sequences of the TAA mitochondria, and identified the orf312 gene specific to this genome, which showed differential expression patterns at different growth stages.
Article
Biochemistry & Molecular Biology
Zihan Liu, Fuqiang Niu, Shaohua Yuan, Shuying Feng, Yanmei Li, Fengkun Lu, Tianbao Zhang, Jianfang Bai, Changping Zhao, Liping Zhang
Summary: This study investigated a new wheat thermo-sensitive cytoplasmic male sterility line, FA99, and found that its fertility conversion was mainly controlled by temperature, with the critical abortion period being the tetrad stage. Transcriptome analysis revealed significant changes in gene expression under different fertility environments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Agronomy
Ranjana Gautam, Pawan Shukla, P. B. Kirti
Summary: Cytoplasmic male sterility (CMS) is a prevalent trait in many plant species and has been utilized in hybrid variety development. Some plants lack usable CMS and fertility restoration systems (Cytoplasmic Genetic Male Sterility Systems-CGMS), requiring the development of male sterile lines through genetic engineering or gene editing. This review provides insights into the development of technologies for hybrid variety production, covering naturally occurring male sterility systems to genetically engineered systems.
THEORETICAL AND APPLIED GENETICS
(2023)