Article
Chemistry, Applied
Darina Pronin, Andreas Boerner, Katharina Anne Scherf
Summary: Analysis of the quantities of four CD-active peptides in 60 German hexaploid winter wheat cultivars from 1891 to 2010 showed that breeding has not significantly altered the immunoreactive potential of wheat. The levels of CD-active peptides were more influenced by harvest year than by cultivar, indicating similar immunoreactive potential in old and modern wheat cultivars.
Article
Immunology
Tian Yu, Yiting Xie, Juanli Yuan, Jinyan Gao, Zhiwen Xiao, Yong Wu, Hongbing Chen
Summary: This study investigated the alleviating effects of resveratrol on celiac disease. Resveratrol was found to reduce intestinal oxidative stress and inflammatory damage caused by wheat gluten in cell and mouse models of celiac disease. It played a role in regulating the development of autoimmunity, nutrient absorption, and the complex response system of oxidative stress, inflammatory response, and immune response in the intestine by activating specific signaling pathways and genes.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Food Science & Technology
Tian Yu, Shuai Hu, Fangfang Min, Jingjing Li, Yunpeng Shen, Juanli Yuan, Jinyan Gao, Yong Wu, Hongbing Chen
Summary: This study provides new evidence for the role of wheat amylase trypsin inhibitors (ATI) in the pathogenesis of celiac disease. The consumption of ATI and gliadin caused intestinal inflammation in mice, and adjuvants enhanced the damage and hyperplasia of intestinal villi. The expression of tissue transglutaminase and IL-15, as well as inflammatory factors, was significantly increased after co-stimulation with ATI and gliadin.
Article
Food Science & Technology
Satoshi Noma, Miki Yamagishi, Yasunari Ogihara, Kanako Kawaura
Summary: This study characterized the alpha-gliadin allele types of Japanese wheat cultivars and found different allele types in the Japanese wheat core collection and commercial cultivars. The diversity of alpha-gliadin alleles in Japanese cultivars was comparable to that of foreign cultivars. Allele frequencies related to end-use were higher in newer varieties, while the frequencies of alleles related to celiac disease were not significantly changed in Japanese cultivars.
JOURNAL OF CEREAL SCIENCE
(2023)
Review
Food Science & Technology
Kalekristos Yohannes Woldemariam, Juanli Yuan, Zhen Wan, Qinglin Yu, Yating Cao, Huijia Mao, Yingli Liu, Jing Wang, Hongyan Li, Baoguo Sun
Summary: Wheat mainly consists of gluten with 30% gliadin and 50% glutenin, which are associated with celiac disease. The challenge lies in discovering new gluten-based peptides with high affinity toward T-cells. The highly immunogenic 33-mer gliadin peptide is considered the main cause, so consistent data on different gluten peptides and their immunogenicity is urgently needed.
FOOD REVIEWS INTERNATIONAL
(2022)
Article
Plant Sciences
Gwyneth Halstead-Nussloch, Tsuyoshi Tanaka, Dario Copetti, Timothy Paape, Fuminori Kobayashi, Masaomi Hatakeyama, Hiroyuki Kanamori, Jianzhong Wu, Martin Mascher, Kanako Kawaura, Kentaro K. Shimizu, Hirokazu Handa
Summary: The study analyzed genomic variation in alpha-gliadins across 11 recently published chromosome-scale assemblies of hexaploid wheat, confirming the presence of two subloci within the Gli-B2 locus and varied immunogenic epitopes among different accessions. Evolutionary analysis identified amino acid sites under diversifying selection, highlighting the potential for improved grain quality and safety through wheat breeding.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Nutrition & Dietetics
Fardowsa Abdi, Saania Zuberi, Jedid-Jah Blom, David Armstrong, Maria Ines Pinto-Sanchez
Summary: A gluten-free diet is essential for treating celiac disease and may improve symptoms in non-celiac gluten/wheat sensitivity. However, adopting a gluten-free diet or a gluten-restricted diet may increase the risk of malnutrition and nutrient deficiencies. Therefore, patients with these conditions should undergo nutritional assessment and monitoring, and receive multidisciplinary care for long-term nutrition management.
Article
Multidisciplinary Sciences
Zhengfu Zhou, Congcong Liu, Maomao Qin, Wenxu Li, Jinna Hou, Xia Shi, Ziju Dai, Wen Yao, Baoming Tian, Zhensheng Lei, Yang Li, Zhengqing Wu
Summary: This study aims to investigate the effect of DNA methylation in the promoter of the gamma-gliadin gene on wheat gluten strength and the proportion of gluten proteins. The results show that the methylation status affects the expression level of the gamma-gliadin gene, thereby influencing the gluten strength and quality of wheat.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Immunology
Xiaoyue He, Donghua Liu, Qicong Chen
Summary: The study identified the significant role of heat shock protein HSP90 in the melanin synthesis pathway of T.marneffei through proteomic analysis. The HSP90 inhibitor geldanamycin demonstrated effective inhibition of melanin production in T.marneffei yeast.
MICROBIAL PATHOGENESIS
(2021)
Review
Biochemistry & Molecular Biology
Charlene B. Van Buiten, Ryan J. Elias
Summary: Celiac disease is an autoimmune disorder characterized by heightened immune response to gluten proteins, with treatment involving complete avoidance of gluten in the diet. Ongoing clinical trials are targeting the immune response and gluten proteins, while recent studies suggest that polyphenols may offer protection by disrupting enzymatic hydrolysis of gluten and exerting anti-inflammatory effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Maria Vittoria Barone, Salvatore Auricchio
Summary: Celiac disease is a chronic inflammatory disease caused by a combination of factors including nutrient-induced T cell response and environmental predisposition to inflammation. Viruses, bacteria, Western diet, and gliadin protein all play a role in the development of the disease, while the anti-inflammatory Mediterranean diet has a protective effect on children with CD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Adam Dios, Bharani Srinivasan, Judit Gyimesi, Katharina Werkstetter, Rudolf Valenta, Sibylle Koletzko, Ilma R. Korponay-Szabo
Summary: Celiac disease is an autoimmune disorder characterized by immune response and antibody production to gluten. TG2 plays a role in the pathomechanism of celiac disease. This study investigates early antibody responses to reveal the development of celiac disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yasmina Rodriguez-Sillke, Michael Schumann, Donata Lissner, Federica Branchi, Fabian Proft, Ulrich Steinhoff, Britta Siegmund, Rainer Glauben
Summary: By analyzing patients with celiac disease and inflammatory bowel disease, it was found that antigen-specific effector T-cells can respond to barrier disruption and antigen translocation in the peripheral blood. Using antigen-reactive T-cell enrichment technique, it was observed that the frequency and phenotype of nutritional antigen-specific T-cells correlated to the presence of intestinal inflammation in patients with Celiac disease and Crohn's disease. These findings suggest that active small intestinal inflammation is crucial for the development of a peripheral food antigen-specific T-cell response in Crohn's disease and celiac disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jorma Isola, Markku Maeki, Martin Hils, Ralf Pasternack, Keijo Viiri, Valeriia Dotsenko, Toni Montonen, Timo Zimmermann, Ralf Mohrbacher, Roland Greinwald, Detlef Schuppan
Summary: In this study, the accumulation of ZED1227 after oral administration of the drug was studied. It was found that ZED1227 mainly accumulated in the villous enterocytes and had an inhibitory effect on TG2 activity. These findings are significant for the treatment of celiac disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Ling Chen, Peipei Su, Guangxiao Yang, Guangyuan He, Chunbao Gao
Summary: Pollen, as the male gametophyte of plants, is crucial for plant sexual reproduction. Investigating pollen-specific promoters can deepen our understanding of pollen development regulation and provide tissue-specific promoters for plant genetic engineering. The PSG076 promoter, previously discovered in wheat, was found to exhibit pollen-specific activity in tobacco. Further analysis showed that this promoter has strong activity specifically at the late pollen development stage, making it a valuable tool for genetic engineering research, such as creating male sterility in wheat and other plant species for hybrid seed production.
PLANT MOLECULAR BIOLOGY REPORTER
(2023)
Article
Materials Science, Ceramics
Miguel Angel Cobos, Patricia de la Presa, Ines Puente-Orench, Irene Llorente, Irene Morales, Asuncion Garcia-Escorial, Antonio Hernando, Jose Antonio Jimenez
Summary: Samples with different inversion parameter values were prepared through mechanical grinding and thermal annealing treatments, and characterized by X-ray, neutron diffraction, and magnetic measurements. The coexistence of short range antiferromagnetic and ferrimagnetic ordering at low temperature was confirmed for the first time, and a different magnetic phase diagram from previous reports was obtained.
CERAMICS INTERNATIONAL
(2022)
Article
Chemistry, Physical
Miguel Angel Cobos, Antonio Hernando, Jose Francisco Marco, Ines Puente-Orench, Jose Antonio Jimenez, Irene Llorente, Asuncion Garcia-Escorial, Patricia de la Presa
Summary: In this study, the antiferromagnetic transition of ZnFe2O4 was investigated, revealing the formation of locally ferrimagnetic phase and coexistence with antiferromagnetic and spin disordered regions when the inversion degree δ is slightly different from zero. The spin disorder disappears under an applied field, and the volume fractions of the three magnetic phases are roughly equal. These results highlight the significant impact of the inversion degree δ on the magnetic properties.
Article
Physics, Condensed Matter
Antonio Hernando, Fernando Giacomone, Jordi Vinolas, Miguel Angel Garcia, Fernando Galvez, Alberto Castellanos, Alfonso de Hoyos, Andres Cerracin
Summary: This report proposes a method for determining the fluctuations of physical and structural properties in metallic ferromagnetic samples using magnetic scanning. The method involves shielding a circular loop electromagnetic field source with a ferromagnetic and metallic sheet. The effectiveness of shielding depends on the field frequency, amplitude, electrical conductivity, and magnetic permeability of the sheet. The proposed method eliminates the need for winding around the sheet, and experimental AC shielding results are provided.
PHYSICA STATUS SOLIDI B-BASIC SOLID STATE PHYSICS
(2022)
Article
Food Science & Technology
Carmen Haro, Maria H. Guzman-Lopez, Miriam Marin-Sanz, Susana Sanchez-Leon, Luis Vaquero, Jorge Pastor, Isabel Comino, Carolina Sousa, Santiago Vivas, Blanca B. Landa, Francisco Barro
Summary: This study characterized the immunogenic epitopes of tritordeum bread and found that it has fewer peptides related to gluten proteins compared to gluten-free bread. Healthy individuals rated tritordeum bread higher and short-term consumption did not have significant effects on gut microbiota diversity or composition.
Article
Agronomy
Eva Hernandez Plaza, Fernando Bastida, David J. Gibson, Francisco Barro, Maria J. Gimenez, Yesica Pallavicini, Jordi Izquierdo, Jose L. Gonzalez-Andujar
Summary: The structure of weed communities, including their composition, taxonomic diversity, and functional diversity, can explain the variability in crop quality. In this study, weed communities in 26 Spanish wheat fields managed either organically or conventionally were analyzed. The relationship between crop quality and weed community variables was examined, taking into account environmental factors. It was found that nitrogen fertilization, previous crop, and precipitation had significant effects on wheat grain protein concentration and composition. The impact of weed communities on grain quality was limited, with only a small increase in the model's explanatory power. Understanding the mechanisms behind weed effects on crop quality can help in designing sustainable weed management practices.
Article
Clinical Neurology
Vanesa Soto-Leon, Eva Diez-Rodriguez, Salvador Herrera-Perez, Juliana M. Rosa, Juan Aguilar, Antonio Hernando, Carlota Bravo-Sanchez, Veronica Lopez-Gonzalez, Yolanda Perez-Borrego, Sven Bestmann, Antonio Oliviero
Summary: This study investigated the effects of transcranial static magnetic field stimulation (tSMS) on the dorsolateral prefrontal cortex (DLPFC) function. It found that tSMS can transiently modulate DLPFC function, specifically affecting inhibitory control and response selection. These findings suggest the potential use of tSMS for the treatment of neuropsychiatric disorders.
CLINICAL NEUROPHYSIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Candelario Palma-Bautista, Jose G. Vazquez-Garcia, Gabriela Lopez-Valencia, Jose Alfredo Dominguez-Valenzuela, Francisco Barro, Rafael De Prado
Summary: Glyphosate resistance in Conyza canadensis has been discovered for the first time in Mexico. Four resistant populations were identified, with two showing moderate resistance and two showing high resistance. The resistant populations had lower glyphosate translocation compared to the susceptible population, and a specific mutation in the EPSPS2 gene was found in two of the resistant populations. This study provides a detailed understanding of glyphosate resistance in C. canadensis and proposes control alternatives.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Candelario Palma-Bautista, Pablo Belluccini, Jose G. Vazquez-Garcia, Ricardo Alcantara-de la Cruz, Francisco Barro, Joao Portugal, Rafael De Prado
Summary: The weed Carduus acanthoides L. has started invading GM crop production systems in Argentina, where glyphosate and 2,4-D are commonly used herbicides. In 2020, a population of C. acanthoides was found to have multiple resistance to both herbicides. The resistance mechanism for glyphosate was a Pro-106 mutation in the EPSPS gene, while resistance to 2,4-D was due to enhanced metabolism mediated by cytochrome P450. This is the first reported case of resistance to glyphosate and 2,4-D in C. acanthoides worldwide.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Plant Sciences
Latifa Chaouachi, Miriam Marin-Sanz, Zayneb Kthiri, Sameh Boukef, Kalthoum Harbaoui, Francisco Barro, Chahine Karmous
Summary: This study aimed to select drought-tolerant durum wheat genotypes from a Landraces gene pool by evaluating various traits under controlled water stress conditions. The results showed that certain landraces exhibited higher tolerance to water stress compared to an improved variety. Chlorophyll fluorescence parameters, relative water content, and electrolyte conductivity were identified as reliable indicators of drought tolerance. The research highlights the potential of local genetic resources in addressing drought stress in durum wheat breeding programs.
Article
Plant Sciences
Miriam Marin-Sanz, Francisco Barro, Susana Sanchez-Leon
Summary: The research found that bread wheat genotypes without the 1BL/1RS translocation have more α- and γ-gliadin epitopes compared to genotypes containing this translocation. The development of low-gluten wheat varieties is a suitable way to fight the increment of pathologies associated with the consumption of cereals. These findings contribute to understanding the immunogenic complexes of α- and γ-gliadins and the development of low-immunogenic wheat varieties through precision breeding programs involving crossing or CRISPR/Cas gene editing.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Antonio Hernando, M. Luisa Ruiz-Gonzalez, Omar Diaz, Jose M. Alonso, Jose L. Martinez, Andres Ayuela, Jose M. Gonzalez-Calbet, Raquel Cortes-Gil
Summary: The presence of La-Mn vacancies in LaMnO3 nanoparticles leads to a significant change in conductivity behavior. The sample remains an insulator overall, showing typical thermal activation mechanism at high temperatures, but exhibits high magnetoconductivity below 200 K. The activation energy decreases linearly with the square of the reduced magnetization and disappears when the sample is magnetized at saturation. Therefore, electron hopping between Mn3+ and Mn4+ contributes largely to the conductivity below the Curie temperature. The influence of the applied magnetic field on conductivity also supports the hypothesis of hopping contribution, and the electric behavior can be explained as an increase in hopping probability via spin alignment.
Article
Biochemistry & Molecular Biology
Jorge Rencoret, Gisela Marques, Mario J. Rosado, Javier Benito, Francisco Barro, Ana Gutierrez, Jose C. Del Rio
Summary: The differences in composition and structure of oat straw lignins from different varieties and planting seasons were studied using various analytical techniques. The results showed that oat straw lignins were mainly composed of guaiacyl and syringyl units, with relatively low amounts of p-hydroxyphenyl units. Significant quantities of p-coumarates and the flavone tricin were also found in the lignins. Moreover, the lignin content and composition of oat straws varied with genotype and planting season. This information is crucial for plant breeding programs and biorefinery applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Agriculture, Multidisciplinary
Candelario Palma-Bautista, Jose G. Vazquez-Garcia, Gabriela Lopez-Valencia, Jose Alfredo Dominguez-Valenzuela, Francisco Barro, Rafael De Prado
Summary: Glyphosate-resistant Conyza canadensis has been found in Mexico, with two moderately resistant populations and two highly resistant populations identified. The resistance was attributed to reduced translocation and a mutation in the EPSPS2 gene. This study provides detailed understanding of glyphosate resistance in C. canadensis from Mexico and proposes control alternatives.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Food Science & Technology
Miriam Marin-Sanz, Susana Sanchez-Leon, Elena Leon, Francisco Barro
Summary: This study compares the gluten and fructan contents of bread sold in artisan bakeries and hypermarkets in Spain. The results show significant differences in gluten and fructan contents among different types of bread, which may contribute to improving strategies for managing gluten and fructans in bread.
FOOD & NUTRITION RESEARCH
(2022)
Article
Agronomy
Miriam Marin-Sanz, Julio C. Masaru Iehisa, Francisco Barro
Summary: The silencing of gliadins in low-gliadin RNA interference (RNAi) wheat lines resulted in a readjustment of grain protein fractions, increased non-gluten proteins, and decreased gluten content. Additionally, fructans linked to non-celiac wheat sensitivity were also significantly reduced in RNAi lines. Up-regulation of genes and transcription factors regulated by ABA may suggest the involvement of this phytohormone in the stress response observed in the RNAi lines.