Comparative Assessment of 5′ A/T-Rich Overhang Sequences with Optimal and Sub-optimal Primers to Increase PCR Yields and Sensitivity

Title
Comparative Assessment of 5′ A/T-Rich Overhang Sequences with Optimal and Sub-optimal Primers to Increase PCR Yields and Sensitivity
Authors
Keywords
DNA amplification, Diagnostics, Primer design, qPCR, <em class=EmphasisTypeItalic >Tobacco mosaic virus</em>, 5′ A/T-rich flap
Journal
MOLECULAR BIOTECHNOLOGY
Volume 55, Issue 1, Pages 17-26
Publisher
Springer Nature
Online
2012-11-02
DOI
10.1007/s12033-012-9617-5

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