Article
Engineering, Environmental
Mengyang Xing, Yong Chen, Wanqin Dai, Xiao He, Boqiang Li, Shiping Tian
Summary: A novel patulin-degrading enzyme preparation was developed, which efficiently degraded the important mycotoxin patulin in fruit-derived products. The immobilized enzyme exhibited high efficiency, stability, safety, and easy separation, establishing the first step in building a bio-detoxification system to control patulin contamination in juice and beverage products.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Endocrinology & Metabolism
Yun Hu, Ying Wang, Ting-ting Cai, Lu Liu, Dong-mei Li, Jian-hua Ma, Bo Ding
Summary: Short-term intensive insulin therapy can increase serum testosterone levels in men with newly diagnosed type 2 diabetes, possibly due to enhanced conversion of DHEA to testosterone.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anirudh P. Shanbhag, Sreenath Rajagopal, Arindam Ghatak, Nainesh Katagihallimath, Ramaswamy Subramanian, Santanu Datta
Summary: Enzymes can be specific or promiscuous catalysts. Through high-throughput screening or site-specific engineering, enzymes can synthesize desired products. Our study aimed to identify promiscuous ketoreductases with the ability to catalyze multiple ketone substrates. Experimental results demonstrated the correlation between the C-terminal lid-loop structure, enzyme flexibility, and turnover rate on propharmaceutical substrates.
BIOCHEMICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Ge Ye, Weihuan Huang, Zeping Chen, Hao Zhong, Junhao Zhong, Xiaoxin Guo, Yuheng Huang, Shruthi Kandalai, Xiaozhuang Zhou, Nan Zhang, Yang Zhou, Qingfei Zheng, Haiyan Tian
Summary: Bufadienolides are toxic components found in amphibious toads and have a wide range of biological activities. This study identified and characterized several 3-epi-bufadienolides with unique structures from the bile of the Asiatic toad. Toxicology assays showed that 3-hydroxyl epimerization significantly decreased the toxicity of bufadienolides. Furthermore, a hydroxyl epimerase enzyme, HSE-1, was discovered in the toad liver, which regulates the toxicity of natural animal-derived products.
Article
Chemistry, Applied
Mengyang Xing, Yong Chen, Boqiang Li, Shiping Tian
Summary: The cloned SDR gene CgSDR can effectively transform patulin into E-ascladiol in vitro, showing significant degradation ability; addition of CgSDR can reduce 80% of patulin in apple juice without affecting its quality.
Article
Chemistry, Medicinal
Ondrej Benek, Michaela Vaskova, Marketa Miskerikova, Monika Schmidt, Rudolf Andrys, Aneta Rotterova, Adam Skarka, Jana Hatlapatkova, Jana Zdarova Karasova, Matej Medvecky, Lukas Hroch, Lucie Vinklarova, Zdenek Fisar, Jana Hroudova, Jiri Handl, Jan Capek, Tomas Rousar, Tereza Kobrlova, Rafael Dolezal, Ondrej Soukup, Laura Aitken, Frank Gunn-Moore, Kamil Musilek
Summary: In this study, new benzothiazolylurea-based inhibitors were developed for 17 beta-HSD10, a potential drug target for Alzheimer's disease and hormone-dependent cancer. The most potent compounds within this class were identified, showing submicromolar inhibitory activity and cell penetrability. These compounds also showed no off-target effects, cytotoxicity, or neurotoxicity. Compound 9, one of the most potent inhibitors, was found to be bioavailable and able to penetrate the blood-brain barrier.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Zhiyong Liu, Rongzhen Zhang, Wenchi Zhang, Yan Xu
Summary: The study successfully improved the catalytic efficiency, product yield, and thermostability of Brucella melitensis 7 alpha-hydroxysteroid dehydrogenase through the design of the I258M variant. The mutation resulted in increased k(cat)/K-M value for chenodeoxycholic acid oxidation, stronger binding to the cofactor, and enhanced enzyme half-life, providing insights into the molecular mechanisms behind these improvements.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Buekre Yaren Ertano, Yeliz Demir, Yahya Nural, Orhan Erdogan
Summary: The study found that acylthiourea compounds have effective inhibitory capacity on critical enzymes associated with diabetes, which contributes to the innovation and development of future diabetes treatment strategies.
Article
Biochemistry & Molecular Biology
Donald Poirier, Rene Maltais, Jacques A. Rousseau, Jenny Roy, Serge Phoenix, Francisco Cortes-Benitez, Roger Lecomte
Summary: A compound that inhibits 17 beta-HSD3 activity and accumulates in prostate tumor tissues has been discovered. This finding opens up the possibility of targeted molecular radiotherapy or radioimaging diagnosis using radiolabelled versions of the compound.
BIOORGANIC CHEMISTRY
(2022)
Article
Microbiology
Xin Zhang, Jiyu Xin, Zhiguo Wang, Wenping Wu, Yutong Liu, Zhenzhen Min, Yueyong Xin, Bing Liu, Jun He, Xingwei Zhang, Xiaoling Xu
Summary: This study determined the structure of full-length MCR from the photosynthetic green non-sulfur bacterium Roseiflexus castenholzii and elucidated the catalytic mechanisms using a combination of molecular dynamics simulations and enzymatic analyses. These findings provide a structural and mechanistic basis for enzyme engineering and biosynthetic applications of the 3-HP carbon fixation pathways.
Article
Plant Sciences
Yanmei Zheng, Yongsheng Zhu, Xiaohui Mao, Minrong Jiang, Yidong Wei, Ling Lian, Huibin Xu, Liping Chen, Huaan Xie, Guodong Lu, Jianfu Zhang
Summary: The study identified a novel lesion mimic gene, SDR7-6, which, when disrupted, led to cell death and adverse effects on multiple agricultural traits. SDR7-6 may play a crucial role at the interface of the two defense pathways against bacterial blight and rice blast disease in rice.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Longhai Dai, Hao Li, Jian-Wen Huang, Yumei Hu, Min He, Yu Yang, Jian Min, Rey-Ting Guo, Chun-Chi Chen
Summary: Patulin, a fatal mycotoxin, is commonly found in contaminated drinking water and fruit-derived products. This study reveals the catalytic mechanism of CgSDR in reducing patulin to the nontoxic E-ascladiol and presents upgraded variants with enhanced activity through structural analysis and engineering. These findings have significant implications for industrial applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Biochemistry & Molecular Biology
Deshuai Lou, Xi Liu, Jun Tan
Summary: This article summarizes the structural characteristics, specificities, biochemical properties, and applications of 7 alpha-hydroxysteroid dehydrogenase and 7 beta-hydroxysteroid dehydrogenase. Emphasis is placed on obtaining novel enzymes that are thermally stable and active under specific conditions.
PROTEIN AND PEPTIDE LETTERS
(2021)
Article
Microbiology
Nicolas Fuentes-Ugarte, Sixto M. Herrera, Pablo Maturana, Victor Castro-Fernandez, Victoria Guixe
Summary: This study reported the structural and kinetic characteristics of glucose-6-phosphate dehydrogenase from Haloferax volcanii, which showed activity in the presence of various salts with maximum activity in KCl. The enzyme's high K-M for glucose-6-phosphate and promiscuous activity for glucose limit its application. Phylogenetic analysis showed that SDR-G6PDH enzymes are exclusively found in Halobacteria.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiaoxiang Hu, Wenjing Liu, Yi Yan, Huaxiang Deng, Yujie Cai
Summary: By immobilizing short-chain dehydrogenase/reductase (SDR) on novel magnetic metal-organic framework nanoparticles via crosslinking, it exhibits better durability, stability and reusability, as well as better kinetic performance in the synthesis of chiral alcohols with high optical purity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemical Research Methods
Yu-Chih Su, Hsing-Yin Chen, Ni Chien Ko, Chi-Ching Hwang, Min Hui Wu, Li-Fang Wang, Yun-Ming Wang, Sheng-Nan Chang, Eng-Chi Wang, Tzu-Pin Wang
ANALYTICAL BIOCHEMISTRY
(2014)
Article
Oncology
Chi-Ching Hwang, Han-Tan Chai, Hsin-Wei Chen, Hsiang-Lin Tsai, Chien-Yu Lu, Fang-Jung Yu, Ming-Yii Huang, Jaw-Yuan Wang
ANNALS OF SURGICAL ONCOLOGY
(2011)
Article
Biochemical Research Methods
Tzu-Pin Wang, Ni Chien Ko, Yu-Chih Su, Eng-Chi Wang, Scott Severance, Chi-Ching Hwang, Ying Ting Shih, Min Hui Wu, Yen-Hsu Chen
BIOCONJUGATE CHEMISTRY
(2012)
Article
Cardiac & Cardiovascular Systems
Thu-Ching Hung, Li-Wen Huang, Shu-Jem Su, Bau-Shan Hsieh, Hsiao-Ling Cheng, Yu-Chen Hu, Yen-Hui Chen, Chi-Ching Hwang, Kee-Lung Chang
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2011)
Article
Agriculture, Multidisciplinary
Chao-Yuan Huang, Jeff Yi-Fu Chen, Jia-En Wu, Yeong-Shiau Pu, Guang-Yaw Liu, Min-Hsiung Pan, Ying-Tang Huang, A-Mei Huang, Chi-Ching Hwang, Shu-Ju Chung, Tzyh-Chyuan Hour
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2010)
Article
Biochemistry & Molecular Biology
San-Cher Chen, Jinn-Yuh Guh, Chi-Ching Hwang, Shean-Jaw Chiou, Tai-Du Lin, Yu-Ming Ko, Jau-Shyang Huang, Yu-Lin Yang, Lea-Yea Chuang
JOURNAL OF CELLULAR BIOCHEMISTRY
(2010)
Article
Cell Biology
Rajalakshmanan Eswaramoorthy, Chih-Kuang Wang, Wen-Cheng Chen, Ming-Jer Tang, Mei-Ling Ho, Chi-Ching Hwang, Hui-Min Wang, Chau-Zen Wang
JOURNAL OF CELLULAR PHYSIOLOGY
(2010)
Article
Chemistry, Organic
Yu-Chih Su, Yu-Lun Lo, Chi-Ching Hwang, Li-Fang Wang, Min Hui Wu, Eng-Chi Wang, Yun-Ming Wang, Tzu-Pin Wang
ORGANIC & BIOMOLECULAR CHEMISTRY
(2014)
Article
Multidisciplinary Sciences
Chi-Ching Hwang, Yi-Hsun Chang, Hwei-Jen Lee, Tzu-Pin Wang, Yu-Mei Su, Hsin-Wei Chen, Po-Huang Liang
Article
Medical Laboratory Technology
San-Cher Chen, Jinn-Yuh Guh, Tai-Du Lin, Shean-Jaw Chiou, Chi-Ching Hwang, Yu-Min Ko, Lea-Yea Chuang
TRANSLATIONAL RESEARCH
(2011)
Article
Chemistry, Multidisciplinary
Tzu-Pin Wang, Yu-Chih Su, Yi Chen, Scott Severance, Chi-Ching Hwang, Yi-Ming Liou, Chia-Hui Lu, Kun-Liang Lin, Rui Jing Zhu, Eng-Chi Wang
Article
Biochemistry & Molecular Biology
Chi-Ching Hwang, Pei-Ru Chang, Chia-Lin Hsieh, Yun-Hao Chou, Tzu-Pin Wang
CHEMICO-BIOLOGICAL INTERACTIONS
(2019)
Article
Chemistry, Physical
Ming-Mao Gong, Chia-Yen Dai, Scott Severance, Chi-Ching Hwang, Bo-Kai Fang, Heng-Bo Lin, Chien-Hui Huang, Chi-Wi Ong, Jeh-Jeng Wang, Pei-Lun Lee, Tzu-Pin Wang
Article
Biochemistry & Molecular Biology
Chi-Ching Hwang, Pei-Ru Chang, Tzu-Pin Wang
CHEMICO-BIOLOGICAL INTERACTIONS
(2017)
Meeting Abstract
Biochemistry & Molecular Biology
Chi-Ching Hwang, Tzu-Jung Huang, Chao-Nan Hsu, Yuan-Liang Wang