Review
Biochemistry & Molecular Biology
Xianhui Liu, Joanna C. Chiu
Summary: O-GlcNAcylation is a nutrient-sensitive protein modification that regulates daily rhythms in biology by modifying circadian clock proteins and downstream effectors. Mistimed feeding disrupts this rhythmic modification and negatively impacts animal biological rhythms and health span.
Article
Biochemistry & Molecular Biology
Peng Zhou, Wen-yi Chang, De-ao Gong, Lu-yi Huang, Rui Liu, Yi Liu, Jie Xia, Kai Wang, Ni Tang, Ai-long Huang
Summary: In hepatocellular carcinoma (HCC), Speckle-type POZ protein (SPOP) is highly O-GlcNAcylated by O-GlcNAc transferase (OGT), which increases the nuclear positioning of SPOP and promotes HCC progression. Ablation of O-GlcNAcylation by an S96A mutation inhibits the cytoplasmic localization of SPOP, suppressing the Nogo-B/c-FLIP cascade and HCC progression.
Article
Biochemistry & Molecular Biology
P. Andrew Chong, Michael L. Nosella, Manasvi Vanama, Roxana Ruiz-Arduengo, Julie D. Forman-Kay
Summary: O-GlcNAc transferase (OGT) is an essential enzyme that adds N-acetylglucosamine to proteins. Predicting glycosylation sites has been challenging due to the lack of experimentally verified sequences. This study identifies a set of sequences that are not glycosylated by OGT and demonstrates a compositional bias for OGT targets. The results also reveal that OGT's tetratricopeptide repeat region recognizes and promotes glycosylation of disordered protein segments.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Cardiac & Cardiovascular Systems
Priya Umapathi, Olurotimi O. Mesubi, Partha S. Banerjee, Neha Abrol, Qinchuan Wang, Elizabeth D. Luczak, Yuejin Wu, Jonathan M. Granger, An-Chi Wei, Oscar E. Reyes Gaido, Liliana Florea, C. Conover Talbot, Gerald W. Hart, Natasha E. Zachara, Mark E. Anderson
Summary: Excessive O-GlcNAcylation contributes to cardiomyopathy, at least in part, due to defective energetics. Enhanced OGA activity is well tolerated, and attenuation of O-GlcNAcylation is beneficial against pressure overload-induced pathologic remodeling and heart failure.
Article
Immunology
Junghwa Seo, Yeolhoe Kim, Suena Ji, Han Byeol Kim, Hyeryeon Jung, Eugene C. Yi, Yong-ho Lee, Injae Shin, Won Ho Yang, Jin Won Cho
Summary: In this study, the researchers found that O-GlcNAcylation plays a suppressive role in erythrocyte necroptosis. Through experiments on mice injected with lipopolysaccharide, it was observed that decreased O-GlcNAcylation led to accelerated erythrocyte necroptosis and increased formation of RIPK1-RIPK3 complex. In addition, the study also discovered that O-GlcNAcylation inhibits the phosphorylation of RIPK1, suppressing its necroptotic activity and the formation of RIPK1-RIPK3 complex in Ripk1(-/-) MEFs.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xin Erica Shu, Yuanhui Mao, Longfei Jia, Shu-Bing Qian
Summary: In eukaryotic cells, eIF3a undergoes dynamic O-GlcNAc modification in response to nutrient starvation, promoting ATF4 reinitiation. Eliminating the modification site induces ATF4 reinitiation, illustrating a mechanism in balancing ribosome recycling and reinitiation.
NATURE CHEMICAL BIOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Ruoting Yin, Xin Wang, Cheng Li, Yuhan Gou, Xuecheng Ma, Yongzhao Liu, Jianfang Peng, Chao Wang, Ying Zhang
Summary: O-GlcNAcylation, a crucial protein modification, plays important roles in various biological processes and aberrant levels are linked to diseases. Utilizing mass spectrometry combined with affinity enrichment can provide insights into the structure-function mechanism of O-GlcNAcylation, aiding in understanding its biological functions.
FRONTIERS IN CHEMISTRY
(2021)
Article
Cardiac & Cardiovascular Systems
Bence Hegyi, Anna Fasoli, Christopher Y. Ko, Benjamin W. Van, Chidera C. Alim, Erin Y. Shen, Marisa M. Ciccozzi, Srinivas Tapa, Crystal M. Ripplinger, Jeffrey R. Erickson, Julie Bossuyt, Donald M. Bers
Summary: Diabetic hyperglycemia is associated with cardiac dysfunction and increased arrhythmia risk, where CaMKII plays a role. O-GlcNAcylation of CaMKII appears to be crucial in promoting arrhythmia susceptibility in diabetic hyperglycemia, while an additional Ang II-NOX2-CaMKII MM281/2 oxidation pathway could further exacerbate the condition.
CIRCULATION RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Aaron T. Balana, Matthew R. Pratt
Summary: Neurodegenerative diseases like Alzheimer's and Parkinson's are highly prevalent and incurable, but there is growing evidence that up-regulation of the posttranslational modification O-GlcNAc can have a protective effect and slow down disease progression.
BIOCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Yalikunjiang Aizezi, Hongming Zhao, Zhenzhen Zhang, Yang Bi, Qiuhua Yang, Guangshuo Guo, Hongliang Zhang, Hongwei Guo, Kai Jiang, Zhi-Yong Wang
Summary: Protein O-glycosylation is an important mechanism for cellular homeostasis, and SPY and SEC are key factors involved in this process in plants. A study identified a SPY O-fucosyltransferase inhibitor (SOFTI) that reduces protein O-fucosylation and affects plant growth and development.
Article
Biochemistry & Molecular Biology
Charlie Fehl, John A. Hanover
Summary: The article discusses the important role of O-GlcNAc modification in cellular metabolism, exploring the challenge of identifying key modification sites among thousands of potential global sites and emphasizing the importance of innovative solutions. Additionally, it summarizes critical suggestions and tool strategies from the international O-GlcNAc community to help newcomers drive innovation in the field.
NATURE CHEMICAL BIOLOGY
(2022)
Article
Chemistry, Analytical
Hyoje Jung, Sang-Hyun Park, Won Ho Yang, Jin Won Cho, Injae Shin
Summary: In this study, we developed five fluorogenic probes to detect O-GlcNAcase in live cells. One of these probes, beta-GlcNAc-FC, was found to be the most effective substrate for O-GlcNAcase. Using this probe, we observed variations in O-GlcNAcase activity in different cell types and its correlation with glucose concentration. Furthermore, ER and mitochondrial stresses did not affect O-GlcNAcase activity.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Pharmacology & Pharmacy
Israel Olapeju Bolanle, Kirsten Riches-Suman, Ritchie Williamson, Timothy M. Palmer
Summary: Protein O-GlcNAcylation has emerged as a new mechanism in the pathogenesis of cardiovascular diseases, playing a vital role in modulating vascular homeostasis and cardiac function through altering cellular functions of target proteins. Studying this mechanism may provide new therapeutic targets for the development of more effective medicines.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Jianing Tang, Guo Long, Kuan Hu, Desheng Xiao, Shuang Liu, Liang Xiao, Ledu Zhou, Yongguang Tao
Summary: This study demonstrates that targeting USP8 can suppress HCC progression and induce ferroptosis. USP8 stabilizes OGT to O-GlcNAcylate SLC7A11, which is essential for SLC7A11 to import cystine from the extracellular environment. Targeting USP8 may be a promising approach for the treatment of HCC.
Article
Chemistry, Multidisciplinary
Jiahui He, Zhiya Fan, Yinping Tian, Weiwei Yang, Yichao Zhou, Qiang Zhu, Wanjun Zhang, Weijie Qin, Wen Yi
Summary: Light control of OGT activity in cells has been achieved by replacing a catalytically essential lysine residue with a genetically encoded photocaged lysine, providing a valuable chemical tool to control cellular O-GlcNAc with spatiotemporal precision and aiding in a better understanding of O-GlcNAc function.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Biotechnology & Applied Microbiology
Tian Liu, Yanwei Duan, Qing Yang
BIOTECHNOLOGY ADVANCES
(2018)
Article
Biochemistry & Molecular Biology
Yanwei Duan, Tian Liu, Yong Zhou, Tongyi Dou, Qing Yang
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Entomology
Lin Liu, Mingbo Qu, Tian Liu, Qi Chen, Xingming Guo, Jun Yang, Qing Yang
JOURNAL OF INSECT PHYSIOLOGY
(2019)
Article
Chemistry, Multidisciplinary
Huibin Yang, Huitang Qi, Tian Liu, Xusheng Shao, Qing Yang, Xuhong Qian
CHINESE CHEMICAL LETTERS
(2019)
Article
Agriculture, Multidisciplinary
Yawen Dong, Song Hu, Xi Jiang, Tian Liu, Yun Ling, Xiongkui He, Qing Yang, Li Zhang
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Lin Liu, Yong Zhou, Mingbo Qu, Yu Qiu, Xingming Guo, Yuebin Zhang, Tian Liu, Jun Yang, Qing Yang
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Fangmeng Chu, Di Wang, Tian Liu, Hongyu Han, Yike Yu, Qing Yang
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2019)
Article
Agriculture, Multidisciplinary
Huitang Qi, Tian Liu, Qiong Lu, Qing Yang
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Article
Chemistry, Medicinal
Xi Jiang, Ashutosh Kumar, Yasutaka Motomura, Tian Liu, Yong Zhou, Kazuyo Moro, Kam Y. J. Zhang, Qing Yang
JOURNAL OF MEDICINAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Tian Liu, Xiaoguang Guo, Yunfei Bu, Yong Zhou, Yanwei Duan, Qing Yang
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2020)
Article
Agriculture, Multidisciplinary
Tian Liu, Hongyu Han, Di Wang, Xiaoguang Guo, Yong Zhou, Tamo Fukamizo, Qing Yang
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Huitang Qi, Xi Jiang, Yi Ding, Tian Liu, Qing Yang
Summary: Kasugamycin, an aminoglycoside antibiotic, has been found to be a competitive inhibitor of glycoside hydrolase family 18 (GH18) chitinases in various organisms. It was discovered that the electrostatic interaction between kasugamycin and a conserved aspartate in GH18 chitinase is vital for its inhibitory activity. This research not only identifies new molecular targets of kasugamycin but also expands the understanding of GH inhibitor design by utilizing a scaffold unrelated to the substrate.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Wenqin Li, Yi Ding, Huitang Qi, Tian Liu, Qing Yang
Summary: The study found that several phytochemicals can inhibit multiple insect chitinolytic enzymes simultaneously, with predicted interactions with key residues and good insecticidal activity. This research provides a new approach for using a multitarget high-throughput screening strategy to discover natural products as insecticide leads.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Qiong Lu, Liping Xu, Lin Liu, Yong Zhou, Tian Liu, Yongxiang Song, Jianhua Ju, Qing Yang
Summary: This study discovered the potential of lynamicin B as a natural-derived pesticide for controlling lepidopteran pests, while leaving its natural enemies unaffected. Lynamicin B showed inhibitory effects on the lepidopteran pest Ostrinia furnacalis, as well as other pests like Mythimna separata and Spodoptera frugiperda.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Tao Chen, Wen-Qin Li, Zheng Liu, Wen Jiang, Tian Liu, Qing Yang, Xiao-Lei Zhu, Guang-Fu Yang
Summary: The study demonstrates that compounds with a biphenyl-sulfonamide skeleton have great potential as OfHex1 inhibitors, showing higher potency than some reported nonglycosyl-based inhibitors. These compounds were found to be competitive inhibitors with respect substrate and exhibited potent drug properties, indicating their potential value in pest management.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)