Article
Biology
Yifeng Zhang, Yingchun Sun, Zhongzi Wu, Xinwei Xiong, Junjie Zhang, Junwu Ma, Shijun Xiao, Lusheng Huang, Bin Yang
Summary: The study analyzed RNA sequence data for subcutaneous fat (SCF), intramuscular fat (IMF), and longissimus dorsi muscle (LDM) samples from 155 F-6 pigs to investigate gene expression levels and network connectivity. Results showed significant differences in gene expression and network connectivity between SCF and IMF, with tissue-specific genes and network modules associated with fat deposition.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Bojiang Li, Yu He, Wangjun Wu, Xiaofan Tan, Zichenhan Wang, David M. Irwin, Zhe Wang, Shuyi Zhang
Summary: This study investigated the circRNA expression profile in the longissimus dorsi muscle of pigs with high and low IMF content. The results identified differentially expressed circRNAs related to lipid metabolism and adipogenesis processes. Among them, circPPARA was found to be positively correlated with IMF content and promoted intramuscular adipogenesis by adsorbing specific miRNAs. These findings provide valuable insights into the regulatory functions of circRNAs in porcine IMF and offer a resource for future circRNA research in pigs.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biology
Yao Jiang, Jiali Liu, Huatao Liu, Wei Zhang, Xiaojin Li, Linqing Liu, Mei Zhou, Jieru Wang, Shiguang Su, Xiangdong Ding, Chonglong Wang
Summary: This study analyzed the gene expression profiles of low and high intramuscular fat (IMF) in Berkshire x Anqing Sixwhite crossbred pigs, and identified a ceRNA (lncRNA4789/miR-381-3p/FABP3) that showed differential expression in the longissimus dorsi tissue. Functional experiments revealed that miR-381-3p inhibits IMF deposition by suppressing preadipocyte differentiation and lipid droplet deposition, while lncRNA4789 rescues FABP3 expression by sponging miR-381-3p. This study provides insights into the molecular mechanisms underlying IMF deposition and potential molecular markers for improving pork quality.
Article
Agriculture, Dairy & Animal Science
Zhiwang Zhang, Tingli Pan, Yu Sun, Siqi Liu, Ziyi Song, Haojie Zhang, Yixing Li, Lei Zhou
Summary: The study showed that calcium addition promotes intramuscular fat accumulation by inhibiting fatty acid oxidation, providing a new approach for increasing intramuscular fat content and improving meat quality.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2021)
Article
Endocrinology & Metabolism
Hui Feng, Tianyi Liu, Salsabeel Yousuf, Xiuxiu Zhang, Wanlong Huang, Ai Li, Lingli Xie, Xiangyang Miao
Summary: Through deep RNA sequencing, we identified differentially expressed lncRNAs, miRNAs, and mRNAs in subcutaneous and intramuscular adipose tissues in pigs with high meat quality. Bioinformatics analysis revealed key lncRNAs, miRNAs, and mRNAs involved in lipid metabolism and adipogenesis. We found that the Wnt signaling pathway plays a critical regulatory role in intramuscular adipogenesis and lipid accumulation.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Pan Zhang, Qinggang Li, Yijing Wu, Yawen Zhang, Bo Zhang, Hao Zhang
Summary: This study used RNA-Seq and proteomics techniques to investigate the key pathways and genes involved in subcutaneous fat and intramuscular fat deposition in Dingyuan pigs. The results showed that the metabolic pathways differed between the backfat group and the IMF group. We identified candidate genes that may be associated with tissue-specific lipid deposition.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Ligang Wang, Zhong-Yin Zhou, Tian Zhang, Longchao Zhang, Xinhua Hou, Hua Yan, Lixian Wang
Summary: The study identified a lincRNA named IRLnc that regulates IMF by influencing the expression of NR4A3. This suggests a novel mechanistic approach for insulin resistance treatment in humans and meat quality improvement in animals.
Article
Agriculture, Dairy & Animal Science
Cai Ma, Yi Sun, Jiying Wang, Li Kang, Yunliang Jiang
Summary: This study revealed the expression and polymorphism of GPAT3 in its promoter region in Laiwu pigs, which is consistent with changes in intramuscular fat content. The expression level of GPAT3 was found to be higher in Laiwu pigs, and a SNP at -1526 site may contribute to IMF deposition by affecting its expression in pigs.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Zoology
Chaogang Yao, Daxin Pang, Chao Lu, Aishi Xu, Peixuan Huang, Hongsheng Ouyang, Hao Yu
Summary: The study found significant differences in the activation of lipid metabolism-related biological processes, including lipogenesis, fatty acid transport, and fatty acid oxidation, between Large White and Min pigs in the PPAR and fatty acid metabolism signaling pathways. The activation of these pathways may play a positive role in reducing IMF content in pigs.
PAKISTAN JOURNAL OF ZOOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Jiaqi Liu, Liyi Wang, Wentao Chen, Jie Li, Tizhong Shan
Summary: The study investigated the effects and regulatory mechanisms of CRTC3 on porcine intramuscular and subcutaneous adipocyte differentiation. Results showed that CRTC3 overexpression significantly altered metabolites in intramuscular adipocytes and promoted adipogenic differentiation by upregulating the Ca2+-cAMP signaling pathway.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Food Science & Technology
Felipe C. Maciel, Otavio R. Machado Neto, Marcio S. Duarte, Min Du, Josiane Fonseca Lage, Priscilla D. Teixeira, Cyntia L. Martins, Edmilson H. R. Domingues, Luiz A. Fogaca, Marcio M. Ladeira
Summary: The study found that administering vitamin A to cattle at birth can increase intramuscular fat content and upregulate the expression of certain genes related to fat metabolism, potentially enhancing carcass marbling without affecting animal performance.
Article
Biotechnology & Applied Microbiology
Yan Liu, Huan Long, Simin Feng, Tingting Ma, Mufeng Wang, Lizhu Niu, Xinyi Zhang, Lianni Wang, Yu Lei, Yilong Chen, Qiankun Wang, Xuewen Xu
Summary: This study identified a correlation between intramuscular fat content and gene expression levels in pigs, highlighting the significant role of the AMPK/PPAR signaling pathway in regulating IMF content. Through a combination of QTL mapping and candidate gene identification, novel genes related to IMF content were proposed.
Article
Chemistry, Applied
Xinhua Hou, Run Zhang, Man Yang, Naiqi Niu, Junchao Wu, Ze Shu, Pengfei Zhang, Lijun Shi, Fuping Zhao, Ligang Wang, Lixian Wang, Longchao Zhang
Summary: Chinese indigenous pig breeds have higher intramuscular fat content and better meat quality. This study investigated the differential metabolites and lipids in the skeletal muscle of Laiwu and Yorkshire pigs, revealing their association with intramuscular fat content and meat flavor. The results showed that the Laiwu pig had a faster lipid droplet formation and contained more triglyceride, reflecting its higher intramuscular fat content.
Article
Veterinary Sciences
Hui Feng, Tianyi Liu, Salsabeel Yousuf, Xiuxiu Zhang, Wanlong Huang, Ai Li, Lingli Xie, Xiangyang Miao
Summary: By studying small RNA libraries constructed from adipose tissues of Laiwu pigs, we identified differentially expressed miRNAs related to lipogenesis and lipid metabolism. These miRNAs were found to be involved in various biological processes and signaling pathways related to adipogenesis and lipid metabolism. We also identified key miRNAs regulating the Wnt signaling pathway through the construction of a miRNA-mRNA interaction network.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Jinhee H. Hwang, John C. Kube, Stephen B. Smith
Summary: The study demonstrated that lubabegron fumarate acts as an antagonist to beta-adrenergic receptor subtypes in subcutaneous adipocytes and exhibits different responses to beta-AA and LUB in subcutaneous and intramuscular adipocytes.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Kamila Pokorna, Jaroslav Citek, Petr Dolezal, Martyna Malopolska, Miroslav Tyra, Monika Okrouhla, Katerina Zadinova, Michal Sprysl, Nicole Lebedova, Roman Stupka
Summary: This study focused on finding a non-invasive method to determine boar taint in pigs by evaluating the concentration of androstenone compounds in their saliva. The results showed a correlation between the concentration of salivary androstenone and the age of the boars. This research has significant implications for preventing the occurrence of boar taint in pork.
Article
Agriculture, Dairy & Animal Science
Angelika Podbielska, Katarzyna Piorkowska
Summary: This study examined the genetic diversity and hybridization between alpacas and llamas based on mitochondrial DNA and the DBY gene. The results showed high levels of mitochondrial DNA introgression and evidence of maternal line hybridization. However, no evidence of hybridization was found in the DBY gene. The study also revealed a high level of genetic diversity in alpacas maintained in Poland.
SMALL RUMINANT RESEARCH
(2022)
Article
Genetics & Heredity
Katarzyna Piorkowska, Kacper Zukowski, Katarzyna Ropka-Molik, Miroslaw Tyra
Summary: The goal of this study was to evaluate the effects of mutations in the FGL1 gene associated with pig productive traits. More than 50 crucial mutations were identified in the FGL1 gene. The association study showed significant effects of the FGL1 on various traits related to fat deposition. The study supports the theory that liver FGL1 is involved in the fat deposition process and provides a potential genetic marker for pig selection.
Article
Biology
Grzegorz Mycka, Adrianna D. Musial, Monika Stefaniuk-Szmukier, Bogusawa Dlugosz, Katarzyna Piorkowska, Agnieszka Bieniek, Tomasz Szmatola, Katarzyna Ropka-Molik
Summary: In this study, the molecular characterization of the PLN gene in horses was performed, and 14 polymorphisms were identified. The results suggest that one of the polymorphisms may be under selection pressure.
FOLIA BIOLOGICA-KRAKOW
(2022)
Review
Nutrition & Dietetics
Anna Steg, Maria Oczkowicz, Grzegorz Smolucha
Summary: There has been a growing interest in dietary supplements in the past two decades. Despite being one of the less-studied substances released for public consumption, advanced techniques such as genomics have made it possible to assess the impact of these supplements on human transcriptome or proteome. This review introduces the main domains of genomics and focuses on three widely used techniques: NGS, LC-MS, NMR, and their applications in studying dietary supplements.
Article
Biochemistry & Molecular Biology
Ewa Tomaszewska, Malgorzata Swiatkiewicz, Siemowit Muszynski, Janine Donaldson, Katarzyna Ropka-Molik, Marcin B. Arciszewski, Maciej Murawski, Tomasz Schwarz, Piotr Dobrowolski, Sylwia Szymanczyk, Slawomir Dresler, Joanna Bonior
Summary: Chronic pancreatitis (CP) is an irreversible and progressive inflammatory disease. This study aimed to investigate the changes in pro-inflammatory cytokines, antioxidant defense system, and selected serum biochemical parameters in a cerulein-induced CP model in pigs. The study found that repetitive cerulein injections not only led to CP and induced inflammation in the intestine, but also impaired the intestinal barrier.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Karolina Zygmunt, Agnieszka Otwinowska-Mindur, Katarzyna Piorkowska, Wojciech Witarski
Summary: It is predicted that by 2040, 35% of meat requirements will be provided through in vitro production. This study focused on developing a medium composition that effectively stimulates the proliferation of bovine satellite cells (BSCs) for cultured meat production. The results indicated that bovine serum and the addition of 10 ng/mL bFGF significantly enhanced the proliferation rate of BSCs, while low glucose content promoted the expression of crucial genes involved in satellite cell self-renewal.
Article
Agriculture, Dairy & Animal Science
Katarzyna Piorkowska, Julia Sroka, Kacper Zukowski, Karolina Zygmunt, Katarzyna Ropka-Molik, Miroslaw Tyra
Summary: This study evaluated the effects of BSCL2 variants on slaughter and fattening characteristics and meat quality traits. The selection of mutations was based on variant calling analysis and chi test results using subcutaneous fat RNA-seq data. Significant genotype/allele distribution variations between high- and low-fat pigs were observed. The study suggests that variant calling from RNA-seq data may help develop genetic markers for complex pig traits, and the BSCL2 G allele (rs341493267) was found to increase backfat thickness in pigs.
Review
Genetics & Heredity
Monika Stefaniuk-Szmukier, Katarzyna Piorkowska, Katarzyna Ropka-Molik
Summary: Equine metabolic syndrome (EMS) is an important issue in modern veterinary medicine, linked to the painful hoof laminitis. We have summarized available information on the genetic background, environmental factors, diagnosis, treatment, and management of EMS for both scientific and practical purposes. Clinical presentation in horses includes obesity, lameness, and insulin dysregulation, with ongoing research in regenerative medicine for promising therapies.
Article
Agriculture, Dairy & Animal Science
Kinga Szczepanik, Maria Oczkowicz, Piotr Dobrowolski, Malgorzata Swiatkiewicz
Summary: Weaning is a stressful period for young animals, leading to oxidative stress and liver damage. Astaxanthin (AST) as a powerful antioxidant has been shown to protect the liver by reducing collagen deposition and regulating gene expression related to liver function.
Article
Agriculture, Dairy & Animal Science
Angelika Podbielska, Katarzyna Piorkowska
Summary: Genotyping-by-sequencing (GBS) is a useful technique for discovering high-quality SNPs and identifying hybrid individuals between different species. GBS can also be applied to finding backcrosses and has unlimited potential for use with restriction enzymes and species without reference genomes.
ANNALS OF ANIMAL SCIENCE
(2022)
Proceedings Paper
Computer Science, Artificial Intelligence
Kuba Chrobocinski, Wojciech Witarski, Katarzyna Piorkowska
Summary: This paper presents an algorithm for automated count and outline of hepatocytes using filters and texture analysis. The algorithm includes a series of filters and algorithms for morphological segmentation, and there are plans for further improvement and development.
INTELLIGENT COMPUTING, VOL 3
(2022)
Article
Agriculture, Dairy & Animal Science
Katarzyna Piorkowska, Kacper Zukowski, Katarzyna Ropka-Molik, Miroslaw Tyra
Summary: This study used pigs as an animal model to investigate the potential gene expression regulation by lncRNAs in fat tissue. The results showed that the expression of hundreds of genes, including 8 lncRNAs, was dependent on fat content. It was also discovered that a transcript related to human insulin resistance was identified, and some lncRNAs may play a regulatory role in gene expression.
ANNALS OF ANIMAL SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Magdalena Szyndler-Nedza, Aurelia Mucha, Katarzyna Ropka-Molik, Katarzyna Piorkowska
Summary: This study aimed to search for new polymorphisms in genes related to lactogenesis and determine their relationship with colostrum and milk composition and piglets' body weight. The study found that polymorphisms in the B4GALT1 gene had a significant effect on colostrum and milk composition and piglets' body weight changes.
ANNALS OF ANIMAL SCIENCE
(2022)
