Article
Biochemistry & Molecular Biology
Yu Shi, Qiong Qu, Chenyang Wang, Yingshi He, Yihua Yang, Yidong Wu
Summary: Insect CYP2 and mitochondrial clan P450 genes are found to have potential roles in chemical defense, in addition to their known involvement in biosynthesis or metabolism of endobiotics. Through in vitro experiments, it was observed that these genes in Helicoverpa armigera can catalyze the conversion of specific compounds into toxins, shedding light on the insect's chemical defense mechanisms. This study provides valuable insights into both insect chemical defense and the physiological functions of these genes in H. armigera and other insects.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kai Tian, Jing Feng, Jiang Zhu, Jiagao Cheng, Mei Li, Xinghui Qiu
Summary: The study found that three paralogous CYP9As were capable of metabolizing multiple pyrethroids, with a greater efficiency in transforming pyrethrin-resembling pyrethroids. These results support the hypothesis that herbivorous insects can cope with synthetic insecticides through their physiological systems.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yu Shi, Qianqian Jiang, Yihua Yang, Rene Feyereisen, Yidong Wu
Summary: P450s of the CYP6B and CYP9A subfamilies in Lepidopteran insects are crucial for host plant adaptation and insecticide resistance, with many paralogs and orthologs being identified in recent genome projects. Functional assays on these enzymes in the generalist herbivore, Helicoverpa armigera, revealed their ability to efficiently metabolize esfenvalerate. The diversity and redundancy of these genes challenge resistance management and pest control strategies.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Peizhuo Wu, Yun Huang, Junyue Zheng, Yu Zhang, Lihong Qiu
Summary: The expression of CYP9A14 and CYP6AE11 related to insecticide tolerance in Helicoverpa armigera is regulated by the CncC pathway, which may be activated by ROS generated by insecticides.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Entomology
Choufei Wu, Chaohui Ding, Shi Chen, Xiaoying Wu, Liqin Zhang, Yuanyuan Song, Wu Li, Rensen Zeng
Summary: Plants release herbivore-induced plant volatiles (HIPVs) to communicate with other organisms in the environment. Insect herbivores can use these odors to upregulate detoxification systems, such as cytochrome P450 monooxygenases (P450s), for tolerance to insecticides. Olfactory exposure to HIPVs enhances larval insecticide tolerance via induction of detoxification P450s.
Article
Entomology
Aniruddha Agnihotri, Naiyong Liu, Wei Xu
Summary: Chemosensory proteins (CSPs) are small, soluble proteins that play a crucial role in transporting odorant and pheromone molecules in the insect chemosensory system. Recent studies reveal that they also function in development, nutrient metabolism and insecticide resistance. In this study, 27 CSP genes were identified from the genome and transcriptome sequences of cotton bollworm, Helicoverpa armigera (Hubner). The expression patterns of these genes were studied by using transcriptomic data obtained from different tissues and stages. The results demonstrate that H. armigera CSP genes are not only highly expressed in chemosensory tissues, such as antennae, mouthparts, and tarsi, but also in the salivary glands, cuticle epidermis, and hind gut. A new method was developed that significantly increased the expression levels of two selected CSPs as soluble recombinant proteins for purification. This study advances our understanding of insect CSPs and provides a new approach to highly express recombinant CSPs in E. coli.
Article
Biochemical Research Methods
Li-Juan Wu, Jun Wu, Hao Lan, Yu Bai, Tong-Xian Liu, He-He Cao
Summary: This study found that homogenizing aphids in a 96-well microplate resulted in higher P450 activities compared to homogenization in Eppendorf tubes. The optimized method of using a microplate for homogenization was found to be applicable to different species of aphids.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Food Science & Technology
Li Zhang, Xiaolong Yang, Rongzheng Huang, Cunxi Nie, Junli Niu, Cheng Chen, Wenju Zhang
Summary: Gossypol, a toxic compound derived from cotton, is harmful to animals. This study successfully degraded free gossypol using an enzymatic method and identified a carboxylesterase enzyme that effectively degrades gossypol. Furthermore, the study elucidated the degradation process of gossypol in vitro.
Article
Biochemistry & Molecular Biology
Ping-Ping Guo, Guo-Cheng Li, Jun-Feng Dong, Xin-Lin Gong, Lingyu Wang, Ke Yang, Jun Yang, Ling-Qiao Huang, Chen-Zhu Wang
Summary: Closely related species Helicoverpa armigera and Helicoverpa assulta have differences in host plant ranges and sex pheromones. The study investigates the genetic basis for gene expression divergence in the antennae of the two species, and explores the connection between gene expression and phenotypic differences. The research findings provide insights into the rapid evolutionary changes in important olfactory traits in moths.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yu Shi, Shuo Sun, Yujun Zhang, Yingshi He, Minghong Du, Andrias O. OReilly, Shuwen Wu, Yihua Yang, Yidong Wu
Summary: This study investigates the functional divergence of genes in Helicoverpa armigera and related species by exchanging amino acids in the substrate recognition sites. The results show that a single amino acid substitution can alter substrate metabolism, providing insights into the process of natural selection of P450 genes and their role in insect-host plant interactions.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Cell Biology
Xiao-Pei Wang, Shu-Peng Sun, Yan-Xue Li, Lin Wang, Du-Juan Dong, Jin-Xing Wang, Xiao-Fan Zhao
Summary: Using the cotton bollworm as a model, researchers found significant changes in hemolymph metabolites during different stages of insect development. Arginine, alpha-ketoglutarate, and glutamate were identified as marker metabolites for larvae, wandering larvae, and pupae, respectively. The hormone 20-hydroxyecdysone played a role in regulating amino acid metabolism by repressing certain enzymes and upregulating others, supporting metamorphic development.
Article
Agronomy
Xinhui Xu, Tingting Li, Lianjun Zhang, Xiaoning Liu
Summary: In this study, a transcription factor called E74B was identified and characterized in the cotton bollworm, Helicoverpa armigera. The results showed that HaE74B plays a critical role in the growth, development, and metamorphosis of the insect. Silencing of HaE74B affected larval molting, growth, and metamorphosis, leading to high larval mortality. This study provides a theoretical foundation for the RNAi-mediated control of this key pest.
PEST MANAGEMENT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Panagiotis Ioannidis, Benjamin Buer, Aris Ilias, Sofia Kaforou, Michalis Aivaliotis, Georgia Orfanoudaki, Vassilis Douris, Sven Geibel, John Vontas, Shane Denecke
Summary: This study investigated the gut of the polyphagous pest Helicoverpa armigera using RNA-sequencing and proteomics. It discovered a relationship between structural homology and expression pattern of sugar transporters in the early larval stages and proposed a potential role for vATPases and SLC9 transporters in the generation of alkaline conditions in the midgut of H. armigera.
Article
Genetics & Heredity
Meenakshi B. Tellis, Bhagyashri Y. Chaudhari, Shweta V. Deshpande, Sanika V. Nikam, Vitthal T. Barvkar, Hemlata M. Kotkar, Rakesh S. Joshi
Summary: This study focuses on the spatio-temporal expression patterns and functional relevance of sugar transporter transcript variants in the lepidopteran model organism, Helicoverpa armigera. The results demonstrate the tissue specificity and developmental stage specificity of these genes, as well as their involvement in sugar metabolism and stress recovery.
Article
Plant Sciences
Jincai Liang, Tianyue An, Jian-Xun Zhu, Shan Chen, Jian-Hua Zhu, Reuben J. Peters, Rongmin Yu, Jiachen Zi
Summary: The characterization of cryptic plant BGCs has shown promise in the discovery of potential agrochemical leads, with a recent study reporting the discovery and characterization of a minimal BGC from the medicinal plant Catharanthus roseus. This BGC contains two genes encoding a terpene synthase and a cytochrome P450, which produce compounds with potent fungicidal activity. The study suggests that conditional coexpression may be sufficient to drive BGC assembly in plants, despite the observed absence of the compounds in C. roseus.
JOURNAL OF NATURAL PRODUCTS
(2021)
