Article
Cell Biology
Francesca Santilli, Jessica Fabrizi, Stefano Martellucci, Costantino Santacroce, Egidio Iorio, Maria Elena Pisanu, Mattea Chirico, Loreto Lancia, Fanny Pulcini, Valeria Manganelli, Maurizio Sorice, Simona Delle Monache, Vincenzo Mattei
Summary: This study analyzed the lipid composition of human Dental Pulp-Derived Stem Cells (DPSCs) and investigated the role of lipid rafts during multilineage differentiation. The results showed that mono-unsaturated fatty acids were the most representative species in the acyl chains of DPSCs. Moreover, disrupting lipid rafts prevented AKT activation and lineage-specific mRNA expression during multilineage differentiation, indicating the importance of lipid raft integrity in DPSCs differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Medicine, General & Internal
Hui-Kuang Huang, Kuang-Kai Hsueh, Yu-Ting Liao, Szu-Hsien Wu, Po-Hsin Chou, Shih-Han Yeh, Jung-Pan Wang
Summary: Infant ADSCs demonstrate superior proliferation rates, antioxidative activity, and differentiation potential compared to UCSCs, with less senescence and replicative stress.
JOURNAL OF THE CHINESE MEDICAL ASSOCIATION
(2023)
Article
Agriculture, Dairy & Animal Science
Hannah J. Stage, Susanne Trappe, Katharina Soellig, Dagmar S. Trachsel, Katharina Kirsch, Cornelia Zieger, Roswitha Merle, Joerg R. Aschenbach, Heidrun Gehlen
Summary: This study investigated the proliferation and multilineage differentiation potential of equine adipose stem cells (ASCs) obtained from different tissue sources. The study found that ASCs had high proliferation and differentiation potential, but were unable to undergo cardiomyogenic differentiation. This study provides a basis for future research in equine medicine.
Article
Multidisciplinary Sciences
Ewan A. Ross, Lesley-Anne Turner, Hannah Donnelly, Anwer Saeed, Monica P. Tsimbouri, Karl V. Burgess, Gavin Blackburn, Vineetha Jayawarna, Yinbo Xiao, Mariana A. G. Oliva, Jennifer Willis, Jaspreet Bansal, Paul Reynolds, Julia A. Wells, Joanne Mountford, Massimo Vassalli, Nikolaj Gadegaard, Richard O. C. Oreffo, Manuel Salmeron-Sanchez, Matthew J. Dalby
Summary: This study reveals that nanotopography can regulate the immunomodulatory capacity of MSCs by reducing intracellular tension and increasing oxidative glycolysis, providing an effective approach for large-scale expansion of functional MSCs for therapeutic purposes.
NATURE COMMUNICATIONS
(2023)
Article
Cell & Tissue Engineering
Katherine Minter-Dykhouse, Timothy J. Nelson, Clifford D. L. Folmes
Summary: During the differentiation process of human-induced pluripotent stem cells (hiPSCs) into cardiomyocytes (CMs), the regulation of cell cycle differs from in vivo mouse cardiac development, resulting in premature exit of hiPSC-CMs from the cell cycle despite their relative immaturity.
STEM CELLS AND DEVELOPMENT
(2022)
Review
Biochemistry & Molecular Biology
Francesca Santilli, Jessica Fabrizi, Fanny Pulcini, Costantino Santacroce, Maurizio Sorice, Simona Delle Monache, Vincenzo Mattei
Summary: This review critically analyzes the role of gangliosides in the differentiation of mesenchymal stem cells and examines their potential use as specific cell surface markers.
Review
Biochemistry & Molecular Biology
Maria Gemma Velasco, Katy Satue, Deborah Chicharro, Emma Martins, Marta Torres-Torrillas, Pau Pelaez, Laura Miguel-Pastor, Ayla Del Romero, Elena Damia, Belen Cuervo, Jose Maria Carrillo, Ramon Cugat, Joaquin Jesus Sopena, Monica Rubio
Summary: In recent years, studies on Muse cells have highlighted their pluripotency, high stress tolerance, self-renewal capacity, DNA repair ability, and non-tumoral nature. Their isolation from adult tissues presents an ethical advantage over embryonic stem cells. Some studies have reported clinical applications of Muse cells in treating diseases such as aortic aneurysm, chondral injuries, and acute myocardial infarction in various animal models and humans. This review provides an overview of Muse cell characterization, biological characteristics, comparison with mesenchymal stem cells, and their therapeutic contributions to different diseases.
Article
Dentistry, Oral Surgery & Medicine
Sinan Mert, Hanna Malyaran, Rogerio B. Craveiro, Michael Wolf, Ali Modabber, Willi Jahnen-Dechent, Sabine Neuss
Summary: Clinical experience suggests that wounds in the maxilla heal faster and more efficiently compared with the mandible. This study aimed to investigate whether differences in the proliferation and differentiation of periodontal ligament stem cells (PDLSC) from the maxilla and mandible contribute to this discrepancy. The results showed that maxillary PDLSC exhibited higher proliferation and differentiation potential compared to mandibular PDLSC, indicating a possible cell-based explanation for the different healing efficacy in the maxilla and mandible.
JOURNAL OF PERIODONTOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Ga Yeon Kim, Gyu Tae Choi, Jinryong Park, Jeongeun Lee, Jeong Tae Do
Summary: In this study, two types of porcine MSCs were compared, and it was found that adipose-derived stem cells showed better proliferation capacity, adipogenic and chondrogenic differentiation potential, and mitochondrial metabolism compared to Wharton's jelly-derived mesenchymal stem cells. This study provides insights into the characteristics of porcine MSCs and their potential applications in animal clinics and cultured meat science.
Article
Endocrinology & Metabolism
Fabiola Marino, Mariangela Scalise, Nadia Salerno, Luca Salerno, Claudia Molinaro, Donato Cappetta, Michele Torella, Marta Greco, Daniela Foti, Ferdinando C. Sasso, Pasquale Mastroroberto, Antonella De Angelis, Georgina M. Ellison-Hughes, Maurilio Sampaolesi, Marcello Rota, Francesco Rossi, Konrad Urbanek, Bernardo Nadal-Ginard, Daniele Torella, Eleonora Cianflone
Summary: Diabetes mellitus affects the biology of cardiac stem/progenitor cells, inhibiting their regenerative potential. By clearing senescent cells, the function of these cells can be restored, resulting in improved cardiac function in diabetic patients.
Article
Biochemistry & Molecular Biology
Eun-Ju Lee, Khurshid Ahmad, Shiva Pathak, SunJu Lee, Mohammad Hassan Baig, Jee-Heon Jeong, Kyung-Oh Doh, Dong-Mok Lee, Inho Choi
Summary: This study introduces novel peptides derived from fibronectin that enhance cell adhesion, proliferation, and differentiation, offering a new approach to cell culture. The peptides showed significant effects on primary cells and stem cells, increasing proliferation and promoting differentiation into specific cell types such as osteoblasts. The results suggest potential applications in the production of core materials for cell culture such as scaffolds and culture medium components.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Lama E. Dawoud, Enas M. Hegazy, Rania A. Galhom, Mervat M. Youssef
Summary: The study evaluates the impact of red LED irradiation on dental pulp stem cells, showing increased cellular growth rates, viability, and differentiation potential towards osteogenic and chondrogenic lineages. Photobiomodulation is considered a promising element of tissue engineering in addition to stem cells, scaffolds, and growth factors.
LASERS IN MEDICAL SCIENCE
(2022)
Article
Cell Biology
Erika N. Guerrero, Shantal Vega, Cindy Fu, Ruth De Leon, Davis Beltran, Mairim Alexandra Solis
Summary: Maternal age influences the biological characteristics and stemness properties of PDMSCs, with cells from mothers aged 22-35 showing higher pluripotency and differentiation effectiveness, while donors aged 26-30 exhibit greater self-renewal ability.
Article
Agriculture, Dairy & Animal Science
Manuela Heimann, Mohamed I. Elashry, Michele C. Klymiuk, Asmaa Eldaey, Sabine Wenisch, Stefan Arnhold
Summary: The investigation of mesenchymal stem cells has become an interesting scientific field in regenerative medicine and organ transplantation. This study focused on establishing an adipogenic differentiation protocol suitable for different mammalian species, and found that using rosiglitazone instead of traditional protocols significantly improves cell viability and adipogenic differentiation capacity. The study also revealed differences between species in terms of fat formation and fat-regulating gene expression. This information is essential for selecting a stem cell specification program and improving clinical targeting of obesity and metabolic disorders in veterinary medicine.
Article
Biotechnology & Applied Microbiology
Hyoungki Kim, Soonjo Kwon
Summary: The study demonstrates that a hypoxic environment, created using specific chemicals in a suspension culture, enhances the proliferation of mouse clonal mesenchymal stem cells and promotes their differentiation into osteogenic cells.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Hulong Le, Xiang Nan, Zhiyong Wang, Lin Gao, Lisi Xie, Chao Zou, Qian Wan, Di Pan, Norman Beauchamp, Xiaoming Yang, Thomas Matula, Bensheng Qiu
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
(2015)
Article
Neurosciences
Qianfa Long, Jianying Li, Qiang Luo, Yue Hei, Kai Wang, Ye Tian, Junle Yang, Hulong Lei, Bensheng Qiu, Weiping Liu
NEUROSCIENCE LETTERS
(2015)
Article
Biochemistry & Molecular Biology
Naisheng Lu, Mengjiao Li, Hulong Lei, Xueyuan Jiang, Weilong Tu, Yang Lu, Dong Xia
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2017)
Article
Engineering, Biomedical
Lin Gao, Lisi Xie, Xiaojing Long, Zhiyong Wang, Cheng-Yi He, Zhi-Ying Chen, Lei Zhang, Xiang Nan, Hulong Lei, Xin Liu, Gang Liu, Jian Lu, Bensheng Qiu
Article
Biology
Lei HuLong, Yu Bing, Yang XueRong, Liu ZeHui, Huang ZhiQing, Mao XiangBing, Tian Gang, He Jun, Han GuoQuan, Chen Hong, Mao Qian, Chen DaiWen
SCIENCE CHINA-LIFE SCIENCES
(2011)
Article
Endocrinology & Metabolism
Xueyuan Jiang, Naisheng Lu, Yun Xue, Suli Liu, Hulong Lei, Weilong Tu, Yang Lua, Yang Lu, Dong Xia
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2019)
Article
Microbiology
Xueyuan Jiang, Naisheng Lu, Haichao Zhao, Hao Yuan, Dong Xia, Hulong Lei
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Agriculture, Dairy & Animal Science
Zehui Liu, Hulong Lei, Renyong Tang, Junhua Yang, Xiulan Guo, Renmao Huang, Qinxiong Rao, Lin Cheng, Zhihui Zhao
Summary: This study found that exposure to different doses of BDE-209 had significant impacts on the growth, blood parameters, carcass traits, and antioxidant capacity in broilers, indicating its toxic effects.
Article
Agriculture, Dairy & Animal Science
Yang Lu, Ruiyang Zhang, Hulong Lei, Yiqiong Hang, Huiqin Xue, Xuan Cai, Yonghong Lu
Summary: This study aimed to investigate the effects of supplementation with fermented feedstuff on the growth performance of weaned pigs and the underlying mechanisms. The results showed that the pigs in the fermented diet group exhibited significantly increased feed intake and growth performance throughout the experimental period. Further analysis revealed that the fermented diet group had higher apparent digestibility of phosphorus and CP compared to the unfermented diet group. There were no significant differences in serum biochemical parameters between the two groups. Additionally, the fermented diet group showed increased gene expression and hormone levels in the jejunum and gastric mucosa.
Article
Reproductive Biology
Naisheng Lu, Xueyuan Jiang, Cong Zhang, Bushe Li, Weilong Tu, Hulong Lei, Wen Yao, Dong Xia
Summary: Researches have shown that melatonin (MLT) can improve oxidative damage and increase sperm motility for conservation sperms, but the mechanism is still unclear. In this study, the energy metabolism of sperm during preservation at 17 degrees C and activation at 37 degrees C was investigated. The results revealed that 1.0 μM MLT treatment affected the energy metabolism of sperm, decreasing ATP content and the mRNA abundance of mitochondrial DNA encoding genes at 17 degrees C, but increasing ATP content and the mRNA expressions of specific genes at 37 degrees C. This study suggests that MLT might regulate sperm ATP generation and antioxidative enzyme activity via MT1 in a temperature-dependent manner.
Proceedings Paper
Engineering, Biomedical
Ke Jiang, Xiaotian Wang, Hulong Lei, Wei Yang, Yin Wu
2013 35TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC)
(2013)
