Article
Multidisciplinary Sciences
Yang Hu, Qi Jiang, Xiang Zhai, Liang Liu, Yuntian Hong
Summary: This study screened and verified the optimal panel of reference genes in colorectal cancer and normal colonic cell lines. The results showed that the expression levels of candidate reference genes varied in different cell lines, but the optimal panel of reference genes always consisted of two genes. Moreover, the optimal combination of reference genes varied among different cell lines.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Multidisciplinary
Lynsey Steel, David M. Ansell, Enrique Amaya, Sarah H. Cartmell
Summary: This study found that PPIA and YWHAZ are stable reference genes in gene expression studies of mesenchymal stem cells under electrical stimulation, while GAPDH and ACTB showed reduced stability at lower seeding densities.
APPLIED SCIENCES-BASEL
(2022)
Article
Plant Sciences
Leandro Francisco de Oliveira, Amanda Rusiska Piovezani, Dimitre A. Ivanov, Leonardo Yoshida, Eny Iochevet Segal Floh, Massuo Jorge Kato
Summary: This study identified stable reference genes for gene expression analysis in four Piper species and provided a list of recommended gene pairs for each species. These findings will facilitate further transcriptomic and functional gene research.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Manish Tiwari, Monika Sodhi, Preeti Verma, Prince Vivek, Ranjit S. Kataria, Saket K. Niranjan, Vijay K. Bharti, Nampher Masharing, Gayatri Gujar, Divya Chanda, Manishi Mukesh
Summary: This study evaluated the expression stability of 10 candidate reference genes in livestock species adapted to high altitude hypoxia conditions. Different panels of reference genes were identified for each species using various statistical algorithms. The findings of this study are important for understanding the molecular basis of high altitude adaptation in native livestock populations.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Gothandapani Sellamuthu, Jan Bily, Mallikarjuna Reddy Joga, Jiri Synek, Amit Roy
Summary: This study evaluated the stability of reference genes in Ips typographus under different experimental conditions and identified ribosomal protein 3a (RPS3-a) as the best reference gene. The findings contribute to future genomic and functional genomic research.
SCIENTIFIC REPORTS
(2022)
Article
Horticulture
Peng Zhou, Linlin Huang, Yingtao Wang, Xiao Li, Xinxin Feng, Liulin Li
Summary: Real-time quantitative PCR (RT-qPCR) is commonly used to explore plant gene expression levels. However, under various stresses and hormone treatments, the levels of most reference genes vary, leading to potential misinterpretation of gene function in pears. This study aimed to identify the optimal reference genes in Huangguan pears and analyze the expression of several candidate genes in different stress and hormone treatments. The results showed that the WDP gene exhibited the most stable expression, ACT was the most stable under MeJA treatment, YLS8 was the most valuable reference gene under ABA hormone and heat stress, and GAPDH showed poor performance compared to other housekeeping genes. These findings provide valuable information for future studies on housekeeping gene selection in pears under biotic and abiotic stresses.
Article
Multidisciplinary Sciences
Pramoda Kumar Sahoo, Sonali Parida, Subhadarshini Parida, Priyashree Parida, Anirban Paul
Summary: Argulosis is a economically significant freshwater fish ectoparasitic disease. Proper selection or normalization of reference genes is crucial for the accuracy of gene expression studies using real-time PCR. This study evaluated seven candidate reference genes and conducted comprehensive gene expression analysis in rohu carp infected with Argulus siamensis. The results identified stable and unstable reference genes and showed the role of immune modulation process in governing infection.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Xiaoxing Yang, Guangxiang Tong, Le Dong, Ting Yan, Huan Xu, Guopan Tang, Yongquan Zhang, Kai Ma, Jiasheng Yin, Youyi Kuang
Summary: This study evaluated the stability of 10 commonly used candidate reference genes in taimen and identified RPS29 and RPL19 as the optimal reference genes for qRT-PCR analysis of gene expression in taimen.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Juliana Mainenti Leal Lopes, Elyabe Monteiro de Matos, Lais Stehling de Queiroz Nascimento, Lyderson Facio Viccini
Summary: Lippia alba is a well-studied plant species known for its medicinal properties, with gene expression studies requiring careful consideration for species with different ploidal levels. This study evaluated the stability of nine candidate reference genes in Lippia alba and identified CIT gene as a suitable stable housekeeping gene for polyploid studies. The results could be used as a reference not only for further gene expression studies in L. alba but also for other polyploid complexes.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Cell Biology
Anelya Kh Alieva, Elena Filatova, Margarita M. Rudenok, Petr A. Slominsky, Maria Shadrina
Summary: Selecting the correct housekeeping genes is crucial for normalizing gene expression data in real-time PCR. In Parkinson's disease, different housekeeping genes are stably expressed in different parts of the brain and in similar tissues of different organisms.
Article
Plant Sciences
D. Sharma, A. Koul, S. Bhushan, S. Gupta, S. Kaul, M. K. Dhar
Summary: MicroRNAs play a crucial role in regulating metabolic pathways in plants and animals. This study focuses on exploring the role of microRNAs in the biosynthesis of carotenoids in tomatoes. The findings suggest that microRNAs regulate carotenoid synthesis at different stages of tomato fruit development, and the identified microRNAs can be utilized to enhance the production of different carotenoids in plants.
Article
Microbiology
Sebastian Gnat, Dominik Lagowski, Aneta Nowakiewicz, Aleksandra Troscianczyk, Mariusz Dylag
Summary: This study examined the suitability of six candidate reference genes for gene expression analysis in the dermatophyte Trichophyton verrucosum cultured in different mycological media. SDHA was identified as the most stable housekeeping gene for future qRT-PCR studies on T. verrucosum strains, suggesting it as an additional good candidate for identification targets in the qRT-PCR technique.
Article
Microbiology
Lingning Meng, Xiaoli Cao, Chuchu Li, Jia Li, Hui Xie, Jiping Shi, Mei Han, Han Shen, Chang Liu
Summary: This study tested the stability of ten classic housekeeping genes under the pressure of eight commonly used antibiotics in the laboratory. Results showed that the stability of gene expression was indeed affected by the types of antibiotics added, and the best reference gene set varied for different antibiotics. This study provides a comprehensive summary of the effects of laboratory antibiotics on the stability of housekeeping genes in P. aeruginosa, highlighting the necessity to select housekeeping genes according to the type of antibiotics used in the initial stage of experiment.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Cell Biology
Do Hyun Kim, Sun Hong Kim, Sang Hi Park, Mi Yeon Kwon, Chae-Yoon Lim, Sun Hwa Park, Kihak Gwon, Se Hwan Hwang, Sung Won Kim
Summary: This study investigated the impact of hypoxic culture conditions on human nasal inferior turbinate-derived stem cells (hNTSCs) and found that under hypoxia, hNTSCs maintained their characteristics and proliferation, but showed changes in their differentiation potential.
Article
Oncology
Syed Rashel Kabir, Tofazzal Islam
Summary: Colorectal cancer is a life-threatening disease in western countries, and finding alternative drugs with less severe side effects is important. This study evaluated the anticancer properties of antimycin A on HCT-116 colorectal cancer cells. Antimycin A showed inhibitory effects on cell proliferation and induced apoptosis in HCT-116 cells. Molecular docking simulation indicated significant binding affinity of antimycin A with several proteins. These findings suggest that antimycin A could be a promising candidate for further anticancer research.
