4.5 Article

Dissection of genetic overlap of drought and low-temperature tolerance QTLs at the germination stage using backcross introgression lines in soybean

Journal

MOLECULAR BIOLOGY REPORTS
Volume 39, Issue 5, Pages 6087-6094

Publisher

SPRINGER
DOI: 10.1007/s11033-011-1423-9

Keywords

Soybean; Backcross introgression lines; Genetic overlap; QTLs; Drought and low-temperature tolerance

Funding

  1. Transgenic Specific Technology program [2009ZX08009-013B]
  2. Chinese Introducing International Super Agricultural Science and Technology program [2006-G1(A)]
  3. Public Agricultural research special funds projects [200903003]

Ask authors/readers for more resources

Northeast of China is the main soybean production area, drought and low-temperature tolerance are both main factors involved in reducing soybean yield and limiting planting regions, the most effective way to solve this problem is to breed cultivars with drought and low-temperature tolerance. A set of the BC2F3 lines was constructed with Hongfeng 11 as recurrent parent and Harosoy as donor parent, and screened in drought and low-temperature condition at the germination stage. Related QTLs were obtained by Chi-test and ANOVA analysis with genotypic and phenotypic data. Eighteen QTLs of drought tolerance and 23 QTLs of low-temperature tolerance were detected. Among them, 12 QTLs were correlated with both drought and low-temperature tolerance, which showed a partial genetic overlap between drought and low-temperature tolerance at the germination stage in soybean. Among the 12 genetic overlap QTLs, Satt253, Satt513, Satt693, Satt240, Satt323, and Satt255 were detected by at least one method for both drought and low-temperature tolerance. Satt557, Satt452, Sat_331, Satt338, Satt271, and Satt588 were detected by only one analysis method. The QTLs detected above were significant loci for drought or low-temperature tolerance in soybean. This will play an important role in MAS for development of both drought and low-temperature tolerance variety.

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