Journal
MOLECULAR BIOLOGY REPORTS
Volume 39, Issue 3, Pages 2337-2345Publisher
SPRINGER
DOI: 10.1007/s11033-011-0984-y
Keywords
F-box protein; Chickpea; Developmental process; Abiotic stress response
Categories
Funding
- Ministry of Education of China
- National Science and Technology Ministry [2006BAD09A04, 2006BAD09A08]
- National Natural Science Foundation of China [30960201, 30860152]
- National Science Foundation for Postdoctoral Scientists of China [20080431107]
- Jiangsu Science Foundation of Postdoctoral Scientists of China [0801048B]
- Xinjiang Science and Technology Department of China [200991254]
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F-box protein family has been found to play important roles in plant development and abiotic stress responses via the ubiquitin pathway. In this study, an F-box gene CarF-box1 (for Cicer arietinum F-box gene 1, Genbank accession no. GU247510) was isolated based on a cDNA library constructed with chickpea seedling leaves treated by polyethylene glycol. CarF-box1 encoded a putative protein with 345 amino acids and contained no intron within genomic DNA sequence. CarF-box1 is a KFB-type F-box protein, having a conserved F-box domain in the N-terminus and a Kelch repeat domain in the C-terminus. CarF-box1 was localized in the nucleus. CarF-box1 exhibited organ-specific expression and showed different expression patterns during seed development and germination processes, especially strongly expressed in the blooming flowers. In the leaves, CarF-box1 could be significantly induced by drought stress and slightly induced by IAA treatment, while in the roots, CarF-box1 could be strongly induced by drought, salinity and methyl jasmonate stresses. Our results suggest that CarF-box1 encodes an F-box protein and may be involved in various plant developmental processes and abiotic stress responses.
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