4.5 Article

The differential expression of peroxisome proliferators-activated receptors in various duck tissues

Journal

MOLECULAR BIOLOGY REPORTS
Volume 37, Issue 3, Pages 1235-1240

Publisher

SPRINGER
DOI: 10.1007/s11033-009-9494-6

Keywords

Peking duck (Anas platyrhynchos); PPAR alpha; PPAR gamma; Clone; Expression pattern

Funding

  1. Eleventh 5-year Program in China [2006BAD14B06-6-1]
  2. Doctor Fund of Northwest AF University

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Peroxisome proliferators-activated receptors (PPARs) plays important role in regulate a variety of target genes which involves in lipid metabolism. In the present study, 1,430 bp of PPAR alpha and 1,650 bp of PPAR gamma in Peking duck (Anas platyrhynchos) were cloned by reverse transcription (RT)-PCR. The cloned sequences of PPAR alpha and PPAR gamma were highly conserved, although relevant changes with respect to human, mouse, chicken, and goose were identified. Real-time quantitative RT-PCR was used to determine if the PPAR alpha and PPAR gamma were expressed in different tissues of male and female Peking ducks. The expression of PPAR alpha and PPAR gamma were widely distributed in all examined tissues. The expression level of PPAR alpha was highest in liver and lowest in kidney in male and female ducks. And it was highest in abdominal fat and lowest in small intestine of PPAR gamma in male and female ducks.

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