Review
Endocrinology & Metabolism
Amanda C. Weiner, Hsiang-Yin Chen, Megan E. Roegner, R. Douglas Watson
Summary: Crustacean Y-organs secrete ecdysteroid molting hormones, which are regulated by ecdysteroids, molt-inhibiting hormone (MIH), and intracellular Ca2+ signaling. Ca2+ signaling appears to play a role in promoting ecdysteroidogenesis during premolt, as evidenced by pharmacological agents affecting intracellular Ca2+ levels and transcriptomic studies showing the abundance of Ca2+ transport proteins in Y-organs.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2021)
Article
Immunology
Nicholas J. MacDonald, Kavita Singh, Karine Reiter, Nguyen Vu, Richard Shimp, Apostolos G. Gittis, Beth Chen, Martin Burkhardt, Baoshan Zhang, Zhixiong Wang, Raul Herrera, Mackenzie Moler, Duck-Yeon Lee, Sachy Orr-Gonzalez, Jessica Herrod, Lynn E. Lambert, Kelly M. Rausch, Olga Muratova, David S. Jones, Yimin Wu, Albert J. Jin, David N. Garboczi, Patrick E. Duffy, David L. Narum
Summary: The development of a malaria vaccine that can block transmission of the parasite from mosquitoes to humans is crucial for malaria elimination efforts. The use of Pfs25, a protein on the surface of the parasite, is being investigated as a transmission-blocking vaccine. Two recombinant forms of Pfs25, Pfs25H and Pfs25M, have been studied for their structural and immunological differences.
Article
Microbiology
Gang Luo, Zijian Geng, Buayisham Kuerban, Yingqing Xu, Jingjing Yang, Jiying Liu, Muwang Li
Summary: In this study, the authors investigated the synergistic effects of YPS1 and YPT7 gene disruption and NAC supplementation on the degradation of recombinant proteins in P. pastoris. The results showed that YPS1 gene disruption reduced protein degradation and increased protein yield. Additionally, YPT7 gene disruption and NAC supplementation had a synergistic effect on protein production.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Virology
Hewen Deng, Mengjiao Li, Qiuyang Zhang, Chencheng Gao, Zhanyun Song, Chunhua Chen, Zhuo Wang, Xin Feng
Summary: Bacillary White Diarrhea, caused by Salmonella pullorum, is a severe disease that leads to high mortality in chicks. Antibiotic resistance has made treatment challenging, so a Salmonella bacteriophage endolysin, LySP2, was explored as a potential treatment option in this study.
Article
Environmental Sciences
Cheng Gui Wang, Wei Wang, Li Li Shi, Yu Chun Shen, Siuming F. Chan
Summary: This research identified two different four-exon CHH genes in lobsters and found that their transcripts produced different variants through alternative splicing. These transcripts were widely expressed in various non-eyestalk tissues of female lobsters and played important roles in the reproductive cycle.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Zuzana Rosenbergova, Zuzana Hegyi, Miroslav Ferko, Natalia Andelova, Martin Rebros
Summary: The deletion of a native signal sequence in plants significantly increased the production of myrosinase in Pichia pastoris, leading to changes in enzyme properties. These findings could provide insights for the expression of other plant enzymes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Entomology
Muhammad Faisal Shahzad, Atif Idrees, Ayesha Afzal, Jamshaid Iqbal, Ziyad Abdul Qadir, Azhar Abbas Khan, Ayat Ullah, Jun Li
Summary: The Cs-Phm gene in the rice striped stem borer plays an important role in ecdysteroidogenesis, regulating the synthesis of molting hormones. Knocking down the Cs-Phm gene through RNA interference leads to decreased ecdysteroid levels, delayed larval development, and higher mortality rates.
Article
Biology
Yoichiro Ito, Misa Ishigami, Goro Terai, Yasuyuki Nakamura, Noriko Hashiba, Teruyuki Nishi, Hikaru Nakazawa, Tomohisa Hasunuma, Kiyoshi Asai, Mitsuo Umetsu, Jun Ishii, Akihiko Kondo
Summary: This study presents a method to improve protein production in the non-conventional yeast Komagataella phaffii. The method involves gene knockout screening, combining gene disruptions, and adaptive evolution to recover growth reduction. The researchers used a multiwell-formatted screening system to evaluate a large number of mutant strains and identified multiple gene disruptions that increased antibody production. They also found that the target protein and promoter could be interchangeable for the effects of gene knockouts. Adaptive evolution was used to recover reduced cell growth caused by multiple gene knockouts and explore the possibility of further enhancing protein secretion. The overall approach holds promise for improving protein production in non-conventional microorganisms.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemical Research Methods
Zhiqian Wang, Yunjun Yan, Houjin Zhang
Summary: Pichia pastoris has traditionally been regulated through methanol induction, which is toxic and flammable. In this study, a novel one-component optogenetic system based on the photosensitive protein EL222 was designed to induce gene expression under blue light and rapidly deactivate it upon switching back to darkness. The intensity of induction in the optogenetic system correlates positively with the dose and periodicity of blue light, and it demonstrates good spatial control.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Parag Goyal, Harish Venkatraman Pai, Phanichand Kodali, Bhavesh Vats, Navratna Vajpai, Shankara Annegowda, Krishnappa Mane, Shamini Mohan, Shruti Saxena, Anil Bangalore Veerabhadraia, Milee Palande, Preethy Sasankan Nair, Digvijay Chandrashekar More, Umamaheshwara Rao Karudumpa, Kunala Jyothirmai, Adroha Bhattacharya, Frida Almeida, Santosh Gulab Khyade, Shankara Gouda, Daniel J. Ranayhossaini, Praveen Reddy Moole, Jeffrey P. Smith, Abhijit Barve, Ramakrishnan Melarkode, Rajesh Ullanat
Summary: MYL-1501D is a biosimilar insulin glargine designed to offer patients greater access to insulin glargine with comparable efficacy and safety to marketed products. Comprehensive studies have shown high similarity in structural and functional properties between MYL-1501D and marketed insulin glargine products, suggesting potential as a cost-effective alternative treatment option.
Article
Biotechnology & Applied Microbiology
Xingxing Liang, Hong Jiang, Xiandong Si, Qi Xin, Di Meng, Peng Chen, Xiangzhao Mao
Summary: The study successfully increased the expression level of plectasin in recombinant Pichia pastoris by constructing a four-copy cassette of plectasin via 2A peptide assembly. This strategy based on 2A peptide assembly can enhance the expression of plectasin and further expand its application prospect.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Lukas Rieder, Katharina Ebner, Anton Glieder, Morten Sorlie
Summary: The study established a method to express fungal LPMOs using a Pichia pastoris platform, achieving high yields and easy purification. It is the first report that a yeast-derived signal peptide can successfully process LPMOs, forming fully functional enzymes.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Biotechnology & Applied Microbiology
Na Zhan, Licong Zhang, Hong Yang, Yalan Zheng, Xinke Wei, Jiajun Wang, Anshan Shan
Summary: This study improved the antimicrobial activity of LL37 by amino acid substitution and dimeric design, successfully expressing recombinant LG with strong antimicrobial activity and low hemolytic activity, as well as improved tolerance to salt ions compared to monomeric peptide FR.
MICROBIAL CELL FACTORIES
(2021)
Article
Engineering, Chemical
Menglin Xiao, Niuniu Chen, Chengzhi He, Shuobo Shi, Qinfu Lu, Shanshan Lv
Summary: The preparation of protoplasts of Saccharomyces cerevisiae and Pichia pastoris using Fe3O4 MNPs was successful, with characterization revealing cell wall breakage and allowing for regeneration, DNA extraction, and transformation. This approach provides a feasible platform for genetic manipulation of yeasts and offers potential for yeast-based biotechnological applications, with the possibility of extension to other microbiomes of industrial importance.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Qi Shen, Xiao-Ting Zhou, Qian Guo, Yu-Zhen Xue, Ya-Ping Xue, Yu-Guo Zheng
Summary: Although widely used for the secretory production of pharmaceutical proteins, P. pastoris has limited success in the secretory production of industrial enzymes. This study identified a signal peptide derived from the PAS_chr3_0030 gene product which conferred secretion competence to certain industrial enzymes.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
