Article
Plant Sciences
Qingqian Zhou, Zhiyuan Fu, Mengyuan Li, Qingwen Shen, Canran Sun, Yijian Feng, Yang Liu, Jianjun Jiang, Tao Qin, Tonglin Mao, Sarah Jane Hearne, Guifeng Wang, Jihua Tang
Summary: This study reveals the crucial role of ZmTFCB, a tubulin folding cofactor, in cell division and cell growth in maize.
Article
Cell Biology
Cecilia D. Gerstner, Michelle Reed, Tiffanie M. Dahl, Guoxin Ying, Jeanne M. Frederick, Wolfgang Baehr
Summary: ARL2 is involved in the neogenesis of tubulin alpha beta-heterodimers with TBCD, and its deletion leads to disrupted microtubule cytoskeleton and absence of outer segments in the retina. However, conditional deletion in rods reveals normal ERG responses. The results suggest a dependence between ARL2 and dynein in generating a functional microtubule cytoskeleton during early photoreceptor development.
Article
Plant Sciences
Yingmei Guo, Yan Chen, Jie Zhang, Jiankun Li, Kaijian Fan, Rongrong Chen, Yunjun Liu, Jun Zheng, Junjie Fu, Riliang Gu, Guoying Wang, Yu Cui, Xuemei Du, Jianhua Wang
Summary: In this study, we cloned a small kernel mutant from maize and found that it was associated with decreased expression and altered methylation levels of the ZmTFCB gene. Further experiments revealed a strong interaction between ZmTFCB and its homolog ZmTFCE. This research highlights the role of epialleles in the regulation of important traits, such as kernel development, in maize.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Biology
Lara Katharina Kruger, Matthieu Gelin, Liang Ji, Carlos Kikuti, Anne Houdusse, Manuel Thery, Laurent Blanchoin, Phong T. Tran
Summary: This study uncovered the regulatory mechanism of mitotic spindle microtubules, including the role of Klp9 in microtubule growth and sliding during spindle shortening, and its interaction with XMAP215/Dis1.
Article
Biochemistry & Molecular Biology
Mathieu Metivier, Emmanuel Gallaud, Alexandre Thomas, Aude Pascal, Jean-Philippe Gagne, Guy G. Poirier, Denis Chretien, Romain Gibeaux, Laurent Richard-Parpaillon, Christelle Benaud, Regis Giet
Summary: The study reveals that the tubulin-specific chaperone dTBCE in Drosophila plays a crucial role in the enrichment of tubulin in the nuclear space after nuclear envelope breakdown, as well as in the promotion of spindle microtubule nucleation, regulated by Ran and lamin proteins. This novel mechanism enhances microtubule nucleation around chromatin during early mitosis, facilitating mitotic spindle assembly.
Article
Pharmacology & Pharmacy
Senlin Li, Siyu Chen, Zhihui Dong, Xingdong Song, Xiuling Li, Ziqi Huang, Huiru Li, Linzhuo Huang, Ganyuan Zhuang, Ran Lan, Mingyan Guo, Wende Li, Phei Er Saw, Lei Zhang
Summary: Platinum-based chemotherapy resistance is associated with elevated tubulin folding cofactor E (TBCE) expression, which contributes to poor prognosis and early recurrence in liver cancer patients. Mechanistically, TBCE silencing affects cytoskeleton rearrangement, leading to increased cisplatin-induced cell cycle arrest and apoptosis. The use of endosomal pH-responsive nanoparticles (NPs) to deliver TBCE siRNA and cisplatin simultaneously reverses chemotherapy resistance and shows superior anti-tumor effects in vitro and in vivo. Summary: NP-mediated delivery of siTBCE and cisplatin effectively reverses platinum-based chemotherapy resistance.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Biochemistry & Molecular Biology
Harriet A. J. Saunders, Dena M. Johnson-Schlitz, Brian Jenkins, Peter J. Volkert, Sihui Z. Yang, Jill Wildonger
Summary: Acetylation of microtubule protein K394 is crucial for synaptic morphogenesis and microtubule stability in the nervous system. The deacetylase HDAC6 regulates K394 acetylation and its overexpression leads to decreased microtubule stability.
Article
Cardiac & Cardiovascular Systems
Panagiotis Efentakis, Michael Molitor, Sabine Kossmann, Magdalena L. Bochenek, Johannes Wild, Jeremy Lagrange, Stefanie Finger, Rebecca Jung, Susanne Karbach, Katrin Schafer, Andreas Schulz, Philipp Wild, Thomas Muenzel, Philip Wenzel
Summary: By conducting a genome-wide association study and using mouse models, this study identified TBCE gene and tubulin homeostasis as novel predictors of vascular function. Knockout of TBCE gene was associated with endothelial dysfunction and increased proliferation of vascular smooth muscle cells mediated by endoplasmic reticulum stress. This research provides a new drug target for ameliorating ER stress-dependent vascular dysfunction.
EUROPEAN HEART JOURNAL
(2022)