Article
Plant Sciences
Minchae Kim, Soongon Jeong, Chae Woo Lim, Sung Chul Lee
Summary: Protein phosphorylation by kinase is crucial for adapting to drought stress conditions, and CaDIMK1 has been identified as a positive modulator of drought tolerance and ABA signal transduction in pepper plants.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Xinwei Liu, Jing Hao, Peng Wei, Xiaohong Zhao, Qiuyan Lan, Lu Ni, Yongzhen Chen, Xue Bai, Ling Ni, Chen Dong
Summary: In this study, the researchers found that SMAD4 plays a critical role in promoting CD8(+) T cell function in both tumor and infection models. SMAD4-mediated transcriptional regulation enhances CD8(+) T cell activation and cytotoxicity through the T cell receptor (TCR) signaling pathway. Furthermore, SMAD4 is directly phosphorylated by ERK following TCR activation.
Article
Cardiac & Cardiovascular Systems
Ting-Ting Zhang, Qing-Qing Lei, Jie He, Xin Guan, Xin Zhang, Ying Huang, Zi-Yue Zhou, Rui-Xin Fan, Ting Wang, Chen-Xi Li, Jin-Yan Shang, Zhuo-Miao Lin, Wan-Li Peng, Li-Kai Xia, Yu-Ling He, Chuan-Ying Hong, Jing-Song Ou, Rui-Ping Pang, Xiao-Ping Fan, Hui Huang, Jia-Guo Zhou
Summary: This study reveals that Best3 mutation, which mainly acts in blood vessels, leads to aortic dissection by regulating the degradation of MEKK2/3, controlling smooth muscle cell phenotypic switch, and maintaining the structural integrity of the aorta. This provides a new therapeutic target for the treatment of aortic dissection.
Review
Biochemistry & Molecular Biology
Mengmeng Zhang, Shuqun Zhang
Summary: This review provides a historic and recent understanding of the role of mitogen-activated protein kinase (MAPK) cascades in plant immunity, response to environmental stresses, and normal growth and development. The signaling specificity of MAPKs in plants is an interesting topic of research and is influenced by factors such as activation kinetics, spatial and temporal expression patterns, and functional characterization of MAPK substrates.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jinpeng Du, Tian Lan, Haotian Liao, Xuping Feng, Xing Chen, Wenwei Liao, Guimin Hou, Lin Xu, Qingbo Feng, Kunlin Xie, Mingheng Liao, Xiangzheng Chen, Jiwei Huang, Kefei Yuan, Yong Zeng
Summary: This study identified the role of circNFIB in intrahepatic cholangiocarcinoma (ICC) by regulating the MEK1/ERK signaling pathway to inhibit tumor growth and metastasis, suggesting its potential as a therapeutic target for ICC treatment.
Article
Biochemistry & Molecular Biology
Ilona Faustova, Kaidi Moll, Ervin Valk, Mart Loog, Mihkel Ord
Summary: Cyclins not only activate CDK complexes, but also serve as docking scaffolds for CDK substrates and inhibitors. G1-cyclins in yeast play a specific role in promoting bud growth and polarization, essential for cell survival. The discovery of a specific docking motif in G1-cyclins expands our understanding of cyclin specificity mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Yuzhen Dan, Nevenka Radic, Marina Gay, Adria Fernandez-Torras, Gianluca Arauz, Marta Vilaseca, Patrick Aloy, Begona Canos, Angel R. Nebreda
Summary: p38α is a protein kinase that regulates cellular responses to various stresses. We identified 35 proteins and 82 phosphoproteins that are modulated by p38α and highlighted the involvement of protein kinases MK2 and mTOR in p38α-regulated signaling networks. Our results provide valuable information on p38α-dependent phosphorylation events in cancer cells and reveal a mechanism by which p38α regulates cell adhesion.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Agriculture, Dairy & Animal Science
Fuqing Huang, Kunling Teng, Yayong Liu, Tianwei Wang, Tianqi Xia, Fangfei Yun, Jin Zhong
Summary: Nisin Z has potential anti-inflammatory effects on mastitis by inhibiting the activation of the ERK1/2 and p38 MAPK signaling pathway and promoting the blood-milk barrier.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jeong Hyeon Lee, Myoung Eun Choi, Hongchan An, Ju Won Moon, Hye Jin Yeo, Youngsup Song, Sung Eun Chang
Summary: BCI-215 effectively inhibits melanogenesis by suppressing the expression of microphthalmia-associated transcription factor (MITF) and its downstream enzymes. This study provides a new therapeutic strategy for treating pigmentation disorders using DUSP-related signaling modulators.
Article
Multidisciplinary Sciences
Wenping Zhou, Wenxue Li, Shisheng Wang, Barbora Salovska, Zhenyi Hu, Bo Tao, Yi Di, Ujwal Punyamurtula, Benjamin E. E. Turk, William C. C. Sessa, Yansheng Liu
Summary: The authors used optogenetics and mass spectrometry-based phosphoproteomics to study the effects of different intensities, durations, and patterns of Akt1 activation on phosphorylation circuits, revealing downstream signaling outcomes of Akt1. The study showed that different intensities, durations, and patterns of Akt1 activation lead to distinct phosphorylation profiles in vascular endothelial cells, and also revealed the interaction between Akt1 signaling and growth factor signaling in these cells. The resulting dataset provides an important resource for future studies on AKT signaling and dynamics.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Hitesh Soni, Ravi Kumar, Praghalathan Kanthakumar, Adebowale Adebiyi
Summary: IL-1 beta induces Ca2+ entry via TRPA1 channels, leading to MAPK-dependent mesangial cell proliferation without stimulating fibronectin production.
Article
Biochemistry & Molecular Biology
Chuwei Lin, Aneirin Alan Lott, Wei Zhu, Craig P. Dufresne, Sixue Chen
Summary: This study used metabolomics and proteomics to investigate the metabolites and protein profiles of the mpk4 mutant and the wild type plants. It revealed the involvement of MPK4 in polyamine synthesis and photosynthesis, providing insights into the metabolic networks potentially regulated by MPK4.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Kyle W. Bender, Daniel Couto, Yasuhiro Kadota, Alberto P. Macho, Jan Sklenar, Paul Derbyshire, Marta Bjornson, Thomas A. DeFalco, Annalise Petriello, Maria Font Farre, Benjamin Schwessinger, Vardis Ntoukakis, Lena Stransfeld, Alexandra M. E. Jones, Frank L. H. Menke, Cyril Zipfel
Summary: Receptor kinases are key in extracellular sensing and stress responses in plants, with leucine-rich repeat receptor kinases regulating responses to internal and external stimuli. The phosphorylation of cytoplasmic domains is crucial for activating RK complexes, but it is not a ubiquitous requirement for LRR-RK activation. Further studies on different protein kinase domains may provide insights into the regulation of LRR-RK complexes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Pharmacology & Pharmacy
Shanelle R. Shillingford, Anton M. Bennett
Summary: Phosphatases and kinases play crucial roles in maintaining the balance between dephosphorylated and phosphorylated proteins for cellular functions. Imbalance or dysfunction in their activities can lead to unfavorable cellular effects and the development of diseases. Protein tyrosine phosphatases (PTPs) are attractive therapeutic targets due to their involvement in cell signaling and diseases, but they have been challenging in drug development. This article focuses on the progress in small-molecule inhibition of the mitogen-activated protein kinase (MAPK) phosphatases (MKPs), discussing successful strategies and inhibitor discovery tools, as well as the potential future of MKP inhibition.
ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY
(2023)
Article
Medicine, Research & Experimental
Enkhtuya Radnaa, Lauren Richardson, Brett Goldman, Jared K. Burks, Tuvshintugs Baljinnyam, Natasha Vora, Hui-Juan Zhang, Elizabeth A. Bonney, Arum Han, Ramkumar Menon
Summary: This study investigated the impact of oxidative stress on p38 MAPK signaling and cell fate, using amnion epithelial cells. The results showed that excessive activation of p38 can have adverse effects on cell behavior and tissue homeostasis.
Article
Agronomy
Xiaolin Tian, Yantao Li, Nan Hao, Xiaozhou Su, Juan Du, Jiajun Hu, Xiangrong Tian
Summary: Triterpenoid saponins from Clematis aethusifolia, especially monodesmosidic saponins with a free 28-COOH group, have the potential to be developed as pesticides to control Plutella xylostella, showing antifeedant, insecticidal, and insect growth inhibitory activities.
PEST MANAGEMENT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Kevin M. Knight, Soumadwip Ghosh, Sharon L. Campbell, Tyler J. Lefevre, Reid H. J. Olsen, Alan Smrcka, Natalie H. Valentin, Guowei Yin, Nagarajan Vaidehi, Henrik G. Dohlman
Summary: Proteins are crucial in signal transduction and pharmacology, with G proteins playing a central role. Research has shown that a conserved network of amino acids dictates the release of G protein subunits, providing insights into the molecular basis of G protein activation.
Review
Cell Biology
Yanfei Jiang, Nan Hao
Summary: Cells can store information from previous environmental conditions, adjusting responses to future challenges and providing fitness advantages. Network motifs like negative feedback loops, positive feedback loops, and feedforward loops underlie cellular memory, enabling memory encoding and storage through their topologies and dynamics.
CURRENT OPINION IN CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shuang Li, Yuanyuan Li, Blake R. Rushing, Sarah E. Harris, Susan L. McRitchie, Daniel Dominguez, Susan J. Sumner, Henrik G. Dohlman
Summary: This study investigates the two glucose receptor systems in Saccharomyces cerevisiae and their distinct roles in glucose signaling.
Article
Agricultural Engineering
Xiaobo Huang, Nan Hao, Genqiang Chen, Shengming Liu, Zhiping Che
Summary: This overview comprehensively summarizes the widespread knowledge regarding the chemistry and biology of nuciferine, including isolation techniques, synthesis, structural modification, bioactivities, metabolism, and biosynthesis. Furthermore, it provides a prospective outlook on future research in this field.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Food Science & Technology
Qiling Chen, Nan Hao, Lili Zhao, Xiangke Yang, Yuxin Yuan, Yuzhu Zhao, Fu Wang, Zuobing Qiu, Ling He, Kan Shi, Shuwen Liu
Summary: In this study, the functions of genes encoding malic enzyme (ME) and malolactic enzyme (MLE) were verified through genetic manipulation in L. plantarum. The results showed that MLE was the only enzyme responsible for direct malate metabolism under acid stress in both O. oeni and L. plantarum. Furthermore, O. oeni exhibited significantly higher malate metabolism activity than L. plantarum under acid stress.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Agricultural Engineering
Xiaobo Huang, Nan Hao, Qun Wang, Ruru Li, Ge Zhang, Genqiang Chen, Shengming Liu, Zhiping Che
Summary: Liriodenine is a non-food bioactive natural product widely found in various forest plants. It exhibits diverse biological effects, including anticancer, antimicrobial, antiparasitic, insecticidal activities, as well as inhibitory effects on enzymes. With its unique molecular structure, Liriodenine holds great potential for development in pharmaceutical and agrochemical industries.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Agricultural Engineering
Yantao Li, Nan Hao, Shengwei Ye, Zilong Hu, Long Zhao, Yinyin Qi, Xiangrong Tian
Summary: This study identified three new triterpenoid saponins from Clematis lasiandra and found that they exhibited significant aphicidal activities against Acyrthosiphon pisum. Compounds 1 and 6 also showed inhibitory effects on digestive enzymes of the pests.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Agronomy
Shengwei Ye, Long Zhao, Yinyin Qi, Han Yang, Zilong Hu, Nan Hao, Yantao Li, Xiangrong Tian
Summary: This study identified azukisapogenol triterpenoid saponins from Oxytropis hirta as effective biopesticides for controlling aphids. These compounds exhibited significant aphicidal activities, including oral toxicity, deterrent effects, and aphid reproduction inhibition.
PEST MANAGEMENT SCIENCE
(2023)
Article
Biology
Julie Paxman, Zhen Zhou, Richard O'Laughlin, Yuting Liu, Yang Li, Wanying Tian, Hetian Su, Yanfei Jiang, Shayna E. Holness, Elizabeth Stasiowski, Lev S. Tsimring, Lorraine Pillus, Jeff Hasty, Nan Hao
Summary: The research on Saccharomyces cerevisiae reveals that a decrease in stability within the ribosomal DNA leads to proteostasis challenge in cells, with ribosomal RNA-binding proteins aggregating upon inhibition of the lysine deacetylase Sir2. These aggregates contribute to proteostasis decline and limit cellular lifespan.
Review
Cell Biology
Andres J. Nevarez, Nan Hao
Summary: This review focuses on the recent advancements in machine and deep learning approaches for studying the metastatic cell state. The authors describe the latest studies using quantitative cell imaging to identify metastatic patterns based on cell appearance, and discuss how these methods can be used to discover hidden drivers in the metastatic cascade and facilitate the development of targeted therapies for metastasis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Zhen Zhou, Yuting Liu, Yushen Feng, Stephen Klepin, Lev S. Tsimring, Lorraine Pillus, Jeff Hasty, Nan Hao
Summary: Synthetic biology allows the design of gene networks for specific biological functions, but it is challenging to engineer the complex trait of longevity. By rewiring a naturally occurring toggle switch, we created a genetic clock that generates oscillations between nucleolar and mitochondrial aging processes, increasing cellular lifespan. These oscillations delay the commitment to aging caused by loss of chromatin silencing or heme depletion. Our study establishes a connection between gene network architecture and cellular longevity, potentially enabling the rational design of gene circuits to slow aging.
Review
Cell Biology
Anders P. P. Mutvei, Michal J. J. Nagiec, John Blenis
Summary: Lysosomes are organelles involved in cellular processes such as degradation of macromolecules, nutrient signaling, and ion homeostasis. Abnormal changes in lysosome abundance are associated with human diseases. This review outlines the mechanisms of lysosome biogenesis and turnover, and discusses the impact of changes in lysosome pool on physiological and pathophysiological processes.
NATURE CELL BIOLOGY
(2023)
Article
Cell Biology
Alan A. Cohen, Luigi Ferrucci, Tamas Fulop, Dominique Gravel, Nan Hao, Andres Kriete, Morgan E. Levine, Lewis A. Lipsitz, Marcel G. M. Olde Rikkert, Andrew Rutenberg, Nicholas Stroustrup, Ravi Varadhan
Summary: Aging biology research is now focused on integrating molecules, cells, genes, and pathways to understand how they collectively contribute to the process of aging. This requires the adoption of a formal complex systems framework and the use of large-scale data collection and new analytical tools. The authors propose that organismal function is achieved through the integration of regulatory mechanisms at multiple hierarchical scales and that disruptions to this integration result in the phenotypic and functional manifestations of aging. They provide examples at different scales and discuss how adopting a complex systems perspective is crucial for advancing our understanding of aging.
Article
Endocrinology & Metabolism
Ana P. Gomes, Didem Ilter, Vivien Low, Stanislav Drapela, Tanya Schild, Edouard Mullarky, Julie Han, Ilaria Elia, Dorien Broekaert, Adam Rosenzweig, Michal Nagiec, Joana B. Nunes, Bethany E. Schaffer, Anders P. Mutvei, John M. Asara, Lewis C. Cantley, Sarah-Maria Fendt, John Blenis
Summary: The study reveals that alterations in propionate metabolism contribute to cancer aggressiveness by causing the accumulation of methylmalonic acid, a by-product of propionate metabolism. Dysregulation of propionate metabolism leads to a pro-aggressive signature in breast and lung cancer cells, increasing their metastatic potential. The downregulation of methylmalonyl coenzyme A epimerase (MCEE) is mediated by an extracellular signal-regulated kinase 2-driven transcription factor Sp1/early growth response protein 1 transcriptional switch driven by metastatic signaling at its promoter level.