Journal
MOLECULAR AND CELLULAR PROBES
Volume 28, Issue 1, Pages 10-12Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.mcp.2013.09.003
Keywords
Cystic fibrosis; Sputum; Pseudomonas aeruginosa; Reference genes; RT-qPCR
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Funding
- University of Bordeaux Segalen
- Institut Polytechnique de Bordeaux
- French association [2010/IC1028]
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The prerequisite to monitor gene expression is the selection of reference genes for normalization of RT-qPCR results. Using 13 sputum samples collected from 9 CF patients, we demonstrated that PA2875 and PA3340 are better reference genes than the previously used clpX and oprL genes. (C) 2013 Elsevier Ltd. All rights reserved.
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