Article
Food Science & Technology
Sabine Delannoy, Mai-Lan Tran, Patrick Fach
Summary: Current methods for screening EHEC in raw milk products often produce false positive results. This study tested new EHEC markers and found that they improved the accuracy of screening and reduced the need for further confirmation. The analysis also revealed differences in the STEC flora among different animal species and suggested the possible presence of a new EHEC serogroup in cow's raw milk cheeses.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Infectious Diseases
Momna Rubab, Deog-Hwan Oh
Summary: The study investigated the antibiotic resistance of Shiga toxin-producing Escherichia coli (STEC) isolates, finding that most strains exhibited phenotypic resistance, with common resistant genes including ere(A), aadA1, and aac(3)-I. These results underscore the importance of monitoring multidrug resistance globally.
Article
Chemistry, Multidisciplinary
Andrea Mancusi, Andrea Fulgione, Santa Girardi, Orlandina Di Maro, Federico Capuano, Yolande T. R. Proroga, Daniela Cristiano
Summary: This study investigated the application of droplet digital PCR (ddPCR) in detecting verocytotoxin-producing Escherichia coli (STEC). The results showed that ddPCR was able to detect STEC in a shorter time compared to the official method, making it a more efficient approach.
APPLIED SCIENCES-BASEL
(2022)
Article
Food Science & Technology
Chanokchon Jaroenporn, Wannakarn Supawasit, Damkerng Bundidamorn, Pathima Udompijitkul, Anunchai Assawamakin, Sudsai Trevanich
Summary: The aim of this study was to validate the developed multiplex PCR-based alternative method for detecting Shiga toxin-producing Escherichia coli (STEC), Listeria monocytogenes (L. monocytogenes), and Salmonella spp. in raw meats. The developed mPCR showed potential as a fast, specific, and sensitive tool for detecting these three bacteria in the raw meat industry.
Article
Food Science & Technology
Rebecca A. A. Bova, Andrew C. C. Lamont, Theodore J. J. Picou, Vincent B. B. Ho, Kristin H. H. Gilchrist, Angela R. R. Melton-Celsa
Summary: Shiga toxins can cross the intestinal barrier and target kidney endothelial cells, and Stx2a translocates more than Stx1a in both single-layer and three-layer cell models. Infection with different strains of STEC affected barrier function differently, with O157:H7 reducing function and O26:H11 allowing translocation without reducing function.
Article
Food Science & Technology
Anja Mueller, Diana Seinige, Nils T. Grabowski, Birte Ahlfeld, Min Yue, Corinna Kehrenberg
Summary: Insects as novel foods are gaining popularity in Europe. While the presence of Shiga toxin-producing E. coli (STEC) in edible insects poses a potential health hazard, the majority of isolates belong to phylogenetic group A, mainly commensal E. coli. Moreover, the low resistance rate among the isolates indicates a low risk for the transmission of antimicrobial-resistant E. coli to consumers.
Article
Immunology
Mariana Izquierdo, Joaquin Lopez, Pablo Gallardo, Roberto M. Vidal, Juan C. Ossa, Mauricio J. Farfan
Summary: This study evaluated the role of Citrobacter werkmanii and Escherichia albertii in the virulence of Shiga toxin-producing and enteroaggregative Escherichia coli pathotypes, revealing their effects on gene expression and toxin secretion.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Istvan Toth, Eva Bagyinszky, Domonkos Svab
Summary: A new molecular detection method has been proposed in this study for rapid, reliable, and easy identification, typing, and phylogenetic classification of Escherichia coli strains in the O157 serogroup. The method can be used in epidemiologic tracing and preliminary phylogenetic grouping of these diverse pathogens.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Xi Yang, Qian Liu, Hui Sun, Yanwen Xiong, Andreas Matussek, Xiangning Bai
Summary: This study identified Stx2l-STEC strains from raw mutton and beef in China, showing that these strains belonged to the same O8 serogroup with diverse host range and geographical distribution. Genomic analysis revealed genetic relatedness between human-derived and food-derived strains, highlighting the need for further investigation into the epidemiological traits and pathogenic potential of Stx2l-STEC strains.
Article
Microbiology
Noble Selasi Gati, Imke Johanna Temme, Barbara Middendorf-Bauchart, Alexander Kehl, Ulrich Dobrindt, Alexander Mellmann
Summary: Hybrid Shiga toxin-producing Escherichia coli strains are positioned between STEC and UPEC, causing both diarrhea and urinary tract infections, with potential to adapt to both intestinal and extraintestinal environments.
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Food Science & Technology
Agnieszka Necel, Sylwia Bloch, Bozena Nejman-Falenczyk, Aleksandra Dydecka, Gracja Topka-Bielecka, Alicja Wegrzyn, Grzegorz Wegrzyn
Summary: This study proposes a validation system for selecting bacteriophages against STEC contamination, and conducts model experiments to explore their potential applications. The results show that bacteriophage vB_Eco4M-7 has a certain bactericidal effect on STEC, but caution should be taken due to the appearance of phage-resistant bacteria.
Article
Microbiology
Alexander Gill, Forest Dussault, Tanis McMahon, Nicholas Petronella, Xiong Wang, Elizabeth Cebelinski, Flemming Scheutz, Kelly Weedmark, Burton Blais, Catherine Carrillo
Summary: This study identified seven STEC isolates with atypical stx2 sequences that do not fit current Shiga toxin taxonomy. The presence of these new Stx subtypes in clinical isolates suggests their potential as human pathogens.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Infectious Diseases
Nicola Mangieri, Roberto Foschino, Claudia Picozzi
Summary: The study found that using bacteriophages can effectively reduce the number of E. coli bacteria producing biofilms, thus improving food safety. Preventing biofilm formation is more effective than trying to remove pre-formed biofilms.
Article
Microbiology
Heather M. M. Blankenship, Stephen E. E. Dietrich, Elizabeth Burgess, Jason Wholehan, Marty Soehnlen, Shannon D. D. Manning
Summary: This study conducted a retrospective analysis of 510 clinical STEC isolates using core genome SNP analysis and found that PFGE and MLST could not fully reveal the genetic diversity and relatedness. SNP analysis was able to cluster isolates based on similarity in PFGE and MLST and uncover their phylogenetic relationships. The use of high-quality SNP analysis enabled a more rapid identification of related strains during outbreak investigations. This study demonstrates how public health laboratories can utilize whole-genome sequencing and phylogenetics to identify related strains and uncover important genetic attributes that can guide treatment practices.
Review
Immunology
Su-bin Hwang, Ramachandran Chelliah, Ji Eun Kang, Momna Rubab, Eric Banan-MwineDaliri, Fazle Elahi, Deog-Hwan Oh
Summary: STEC is a global foodborne bacterial pathogen that can cause severe diarrhea and other critical illnesses, mainly through inflammation caused by Shiga toxins. There is currently no widely accepted treatment for STEC infections, and further research is needed.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Michelle Wemette, Amelia Greiner Safi, Wendy Beauvais, Kristina Ceres, Michael Shapiro, Paolo Moroni, Francis L. Welcome, Renata Ivanek
Article
Biotechnology & Applied Microbiology
Alexandra M. Belias, Adrian Sbodio, Pilar Truchado, Daniel Weller, Janneth Pinzon, Mariya Skots, Ana Allende, Daniel Munther, Trevor Suslow, Martin Wiedmann, Renata Ivanek
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Veterinary Sciences
Barbara Delvescovo, Kathleen R. Mullen, Steven W. Eicker, Renata Ivanek, Dorothy M. Ainsworth
Summary: This study aimed to investigate whether dysphagic foals were less likely to race as adults, and if their athleticism differed from healthy foals in terms of age of first race, Earnings Per Start Index, and Speed Index. The results showed that formerly dysphagic foals did not exhibit negative impacts on their athleticism compared to normal foals.
EQUINE VETERINARY JOURNAL
(2021)
Article
Immunology
Lin Teng, Shinyoung Lee, Dongjin Park, Kwangcheol Casey Jeong
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Eliot Stanton, Taylor A. Wahlig, Dongjin Park, Charles W. Kaspar
Editorial Material
Environmental Sciences
Bin Cao, April Z. Gu, Pei-Ying Hong, Renata Ivanek, Baikun Li, Aijie Wang, JingYi Wu
ENVIRONMENTAL RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
M. Wemette, A. Greiner Safi, A. K. Wolverton, W. Beauvais, M. Shapiro, P. Moroni, F. L. Welcome, R. Ivanek
Summary: This study explored the US public's perceptions of antibiotic use in dairy farming, finding that 90.7% of respondents believed antibiotics posed a threat to human health, and 71.5% were willing to pay more for milk produced without antibiotics. In addition, consumers' willingness to pay more for antibiotic-free milk was associated with factors such as their beliefs about antibiotic use and cattle treatment, household income, place of birth, social ideology, and marital status.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Food Science & Technology
Ece Bulut, Alison Stout, Michelle Wemette, Sebastian Llanos-Soto, Robert C. Schell, Amelia Greiner Safi, Michael A. Shapiro, Paolo Moroni, Renata Ivanek
Summary: This study investigated the US public's perceptions of organic dairy farming and antibiotic use on dairy farms, finding that consumers' knowledge about antibiotic use does not affect their purchasing behavior. However, participants familiar with the regulations of antibiotic use were more likely to oppose the US organic standard on antibiotic use.
JOURNAL OF FOOD SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Genevieve Sullivan, Claire Zoellner, Martin Wiedmann, Renata Ivanek
Summary: Food facilities require time- and cost-saving methods for environmental monitoring of pathogens. In silico modeling can help in rapidly evaluating different sampling schemes for pathogen detection. Two agent-based models were developed to simulate Listeria dynamics in fresh-cut produce facilities, with results showing that sampling schemes based on zone 3 or model predictions may overestimate contamination prevalence, while random sampling may be more useful for reflecting true prevalence.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Infectious Diseases
Ece Bulut, Renata Ivanek
Summary: This study reviewed and compared the methodologies used by the FDA, ESVAC, PHAC and OIE to adjust antimicrobial sales for use in food animals in the USA. The results showed substantial differences in the estimates of biomass-adjusted antimicrobial sales among the four methodologies.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Nutrition & Dietetics
Ji-Hyun Bae, Dongjin Park
Summary: This study identified genetic loci associated with osteoporosis and found that these loci are influenced by individual's gender and dietary calcium intake.
CLINICAL NUTRITION
(2022)
Review
Food Science & Technology
Alexandra Belias, Genevieve Sullivan, Martin Wiedmann, Renata Ivanek
Summary: This review identified the locations, risk factors, and interventions for persistent Listeria in food processing facilities, with a focus on the need for additional research in produce operations. The study also highlighted the variability in the definition of persistence among studies and emphasized the importance of standardizing this definition.
Article
Agriculture, Dairy & Animal Science
Robert Charles Schell, Ece Bulut, Hannah Padda, Amelia Greiner Safi, Paolo Moroni, Renata Ivanek
Summary: Concerns about antibiotic resistance have led to calls for reducing antibiotic use in livestock, including dairy cattle, in the United States. While universal organic farming would be impractical, a proposed alternative is to label milk with responsible antibiotic use (RAU), which minimizes antibiotics more than conventional milk and has better animal welfare than organic milk. However, consumers are unwilling to pay a significant premium for RAU-labeled milk compared to unlabeled milk, indicating a potential contradiction between consumer preferences and actual purchasing behavior.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Cecil Barnett-Neefs, Martin Wiedmann, Renata Ivanek
Summary: Persistent Listeria monocytogenes contamination in packinghouses can be difficult to remove and may contaminate produce. Simulation-based decision support tools were developed to predict contamination sites and simulate corrective actions. The study found that facility-specific, risk-based sanitation methods were effective in reducing persistence, and hourly contamination patterns could be mistaken for persistence depending on sampling frequency.
JOURNAL OF FOOD PROTECTION
(2022)
Article
Biotechnology & Applied Microbiology
Maria T. Brandl, Renata Ivanek, Ana Allende, Daniel S. Munther
Summary: Weather factors greatly influence bacterial behavior on plants, and a novel model based on weather factors and bacterial state was developed to predict population dynamics on leafy vegetables. The model accurately predicted population sizes of pathogenic E. coli and S. enterica on lettuce and cilantro leaves under various conditions. The model showed potential in predicting contamination risk in the field, highlighting the importance of weather-based models in ensuring microbial safety of produce. Rating: 9/10
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)