Article
Medicine, General & Internal
Noreafifah Semail, Azian Harun, Ismail Aziah, Nik Mohd Noor Nik Zuraina, Zakuan Zainy Deris
Summary: Despite the advanced understanding of melioidosis, caused by Burkholderia pseudomallei, it continues to be a global concern. This study has developed a sensitive and rapid multiplex PCR assay to detect the virulence gene cluster of B. pseudomallei, which shows high sensitivity and specificity.
Article
Chemistry, Analytical
Jilien Michelle Wong Tzeling, E. A. R. Engku Nur Syafirah, Ahmad Adebayo Irekeola, Wardah Yusof, Nurul Najian Aminuddin Baki, AbdelRahman Zueter, Azian Harun, Yean Yean Chan
Summary: This study developed a multiplex real-time loop-mediated isothermal amplification assay using a dual-function oligonucleotide (DFO) that can monitor signals and accelerate amplification simultaneously. The assay demonstrated 100% specificity and sensitivity in detecting Burkholderia pseudomallei with low detection limits in different samples. The development of an integrated multiplex LAMP and real-time monitoring system simplifies the workflow for rapid and specific nucleic acid diagnostic tests.
ANALYTICA CHIMICA ACTA
(2021)
Review
Veterinary Sciences
Hanka Brangsch, Harisankar Singha, Karine Laroucau, Mandy Elschner
Summary: Although glanders has been eradicated in most developed countries, it still persists in various countries, causing economic losses to the equine industry. The genetic diversity and global distribution of the causing agent, Burkholderia (B.) mallei, have not been fully assessed due to its high clonality. PCR-based methods and genotyping using tandem repeat regions are established for distinguishing and typing B. mallei. The development of sequencing techniques enables the use of whole genome sequencing data for higher genomic resolution, but the high GC content of B. mallei limits obtaining complete genomic sequences.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Infectious Diseases
Marcus V. D. Falcao, Karine Laroucau, Fabien Vorimore, Thomas Deshayes, Vania L. A. Santana, Karla P. C. Silva, Sergio A. do Nascimento, Roberto S. de Castro, Flabio R. Araujo, Rinaldo A. Mota
Summary: This study analyzed different Burkholderia mallei isolates from glanderous horses in Brazil and identified different clusters of strains, suggesting multiple introductions of glanders in the country, potentially linked to strains from Europe.
INFECTION GENETICS AND EVOLUTION
(2022)
Article
Biochemical Research Methods
Guanfeng He, Yu Zeng, Qizhong He, Tuxuan Liu, Nanhong Li, Hui Lin, Muhong Zeng, Yonglong Li, Min Peng, Junfen Cheng, Wang Liu, Weimin Yao
Summary: We report a case of glanders in a 60-year-old male with a history of diabetes. The patient presented with cough, expectoration, and fever. CT imaging revealed B. mallei infection in the lung and lymph nodes, and inflammation in the lower lobes of both lungs. Diagnosis was confirmed by lymph node biopsy and bronchoalveolar lavage multiplex PCR-based targeted gene sequencing. The patient showed improvement after treatment with meropenem.
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
(2023)
Article
Chemistry, Multidisciplinary
Dan Huang, Hui Deng, Juan Zhou, Guan A. Wang, Qian Lei, Chen Guo, Wanting Peng, Peng Liang, Chenlan Shen, Binwu Ying, Weimin Li, Feng Li
Summary: PANDA technology eliminates the interference caused by high concentrations of wild-type sequences and enables the ultrasensitive detection of cancer mutations with low VAFs. Compared to digital PCR, PANDA has fewer false negatives and lower costs. It also offers multiplex testing and has wide applications in analyzing rare mutations in diverse diseases.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Microbiology
Paula A. Pereira Suniga, Cynthia Mantovani, Maria G. Santos, Juliana S. Gomes Rieger, Emanuelle B. Gaspar, Fernando Leandro dos Santos, Rinaldo A. Mota, Karla P. Chaves, Andrea A. Egito, Jose Carlos O. Filho, Alessandra F. Castro Nassar, Lenita Ramires dos Santos, Flabio R. Araujo
Summary: This study demonstrated the detection of Burkholderia mallei in equids with positive serology for glanders in all five geographic regions of Brazil using species-specific PCR and sequencing. The use of this method expands the possibility of strain isolation and epidemiological characterizations based on molecular information. The microbiological detection of B. mallei in cultures from asymptomatic equids raises the possibility of environmental elimination of the agent.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Seda Ekici, Orhan Dudakli, Dilek Dulger, Maksut Murat Maden, Ayse Demirhan
Summary: The National Glanders Eradication Project successfully eradicated Glanders in our country between 2000-2001. However, in December 2019, 81 horses were culled in Turkiye due to the detection of an epidemic in horses in Buyukada. Glanders cases were also reported in horses in Usak and Bolu in 2019. The use of PCR for the reliable and fast detection of Burkholderia mallei in horses could prevent the future spread of infections in Turkiye.
ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
(2023)
Article
Polymer Science
Boris Shaskolskiy, Ilya Kandinov, Dmitry Kravtsov, Alexandra Vinokurova, Sofya Gorshkova, Marina Filippova, Alexey Kubanov, Victoria Solomka, Dmitry Deryabin, Ekaterina Dementieva, Dmitry Gryadunov
Summary: A multiplex assay using a low-density hydrogel microarray is developed to identify genomic substitutions in N. gonorrhoeae determining resistance to treatment agents. The microarray accurately identifies drug resistance determinants and single-nucleotide substitutions, with results consistent with Sanger sequencing. Analysis of clinical isolates in Russia over 5 years demonstrates the utility of hydrogel microarrays in monitoring antibiotic resistance spread.
Article
Infectious Diseases
Nawarat Somprasong, Johannah P. Hagen, Jason W. Sahl, Jessica R. Webb, Carina M. Hall, Bart J. Currie, David M. Wagner, Paul Keim, Herbert P. Schweizer
Summary: This study found that Burkholderia pseudomallei and Burkholderia mallei are the only Burkholderia strains that contain the 70STSK73 PenA Ambler motif, which may explain their inherent sensitivity to Amoxicillin-clavulanic acid (AMC). The authors also developed a specific PCR assay to amplify the penA gene, allowing reliable confirmation of the presence of B. pseudomallei.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Microbiology
Maryam Shafipour, Hesamaddin Shirzad-Aski, Ezzat Allah Ghaemi, Ahmad Sohrabi, Maya Babaii Kochaksaraei, Masoume Taziki, Somayeh Rahimi, Kiarash Ghazvini, Basireh Baei
Summary: This study evaluated the application of SNP technique for lineage identification of M. tuberculosis, compared with MIRU-VNTR method. The results showed that SNP typing method has the potential as a rapid laboratory screening test for determining the lineages of M. tuberculosis.
CURRENT MICROBIOLOGY
(2021)
Article
Immunology
Jin Li, Qiu Zhong, Mei-Yun Shang, Min Li, Yuan-Su Jiang, Jia-Jun Zou, Shan-Shan Ma, Qing Huang, Wei-Ping Lu
Summary: In this study, a simple and visual device combined with lateral flow strip-based recombinase polymerase amplification (LF-RPA) was developed for the identification of Burkholderia pseudomallei. The LF-RPA assay showed good performance in amplifying target DNA at a temperature range of 30-45 degrees C and completed the entire reaction in a short time. It had a low limit of detection for B. pseudomallei genomic DNA, comparable to real-time PCR. The newly developed LF-RPA system was also successfully used to retrospectively confirm clinical B. pseudomallei isolates.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Tamara Kutateladze, Kakha Bitskinashvili, Nelly Sapojnikova, Tamar Kartvelishvili, Nino Asatiani, Boris Vishnepolsky, Nelly Datukishvili
Summary: This study developed an effective multi-allergen diagnostic approach for assessing transgenic soybeans, using multiplex PCR coupled with DNA chip technology. The method was shown to be efficient for simultaneous identification of major allergens in herbicide-resistant GMOs, providing a fast and accurate testing method for food allergens.
Article
Microbiology
Veysel Uzun, Figen Celik, Sami Simsek, Harun Kaya Kesik, Seyma Gunyakti Kilinc, Xiaocheng Zhang, Haroon Ahmed, Jianping Cao
Summary: This study is the first to report the existence of hybrid Fasciola flukes and 83 unique haplotypes of F. hepatica in Turkey.
Article
Microbiology
Karuppusamy Shanmugasundaram, Harisankar Singha, Sheetal Saini, Bhupendra N. Tripathi
Summary: This study assessed the phylogenetic relationships between glanders isolates from different states in India and found genetic diversity between recent and older isolates. The study also suggested that glanders outbreaks in different states may be caused by the migration of infected animals. The 16S rDNA and ITS region may be used for molecular characterization of B. mallei associated with glanders in resource-limited settings.
CURRENT MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Maximilian Collatz, Florian Mock, Emanuel Barth, Martin Hoelzer, Konrad Sachse, Manja Marz
Summary: This study introduces EpiDope, a Python tool using deep neural network for detecting linear B-cell epitope regions on individual protein sequences. With an AUC above 0.67 in the ROC curve, EpiDope outperforms other currently used linear B-cell epitope prediction tools. The software shows reliable prediction of linear B-cell epitopes, contributing to a significant reduction in laboratory experiments and costs.
Article
Nanoscience & Nanotechnology
Amjad Islam Aqib, Muhammad Saqib, Shanza Rauf Khan, Tanveer Ahmad, Syed Ahsan Raza Shah, Muhammad Aamir Naseer, Muhammad Shoaib, Iqra Sarwar, Muhammad Fakhar-e-Alam Kulyar, Zeeshan Ahmad Bhutta, Iqra Muzammil, Tanzeel Riaz, Mumtaz Ali Khan, Mahboob Ali, Khadija Yasmeen
Summary: The current study focused on resistance modulation of dairy-linked epidemic methicillin-resistant Staphylococcus aureus using plant extracts, NSAIDs, and microparticles. The study found a percentage of subclinical samples positive for S. aureus in dairy milk and investigated the association of risk factors with the pathogen. Antimicrobial assays showed resistance to multiple antibiotics and the potential synergistic effects of combinations with different substances. The findings suggest the need for alternative antimicrobial sources against pathogenic S. aureus.
APPLIED NANOSCIENCE
(2021)
Article
Veterinary Sciences
Muhammad Hammad Hussain, Muhammad Nadeem Asi, Sheikha Saif Rashid Al-Uahmadi, Afrah Hamed Abdullah Al-Subhi, Narjis Yousaf Ali Al-Senaidi, Ruqiyah Salem Nasser Al-Subhi, Muzna Khalifa Ismail Al-Beloushi, Fatma Salim Said Al-Sinani, Bushra Salim Thani Al-Riyami, Muhammad Khalid Mansoor, Muhammad Saqib, Hatim Ali El-Tahir, Salim Suleman Al-Makhladi, Abdulamjaeed Hamood Al-Rawahi, Mahir Gharib Al-Maawali
Summary: The seroepidemiology of CCPP in small ruminants in Oman showed varying prevalence rates in different governorates, with goats having a significantly higher infection rate than sheep. At the flock level, open herds, coastal locations, and flock sizes of more than 100 animals were identified as significant risk factors for CCPP. At the animal level, goats and small ruminants above the age of one year were found to be more likely to acquire CCPP.
PAKISTAN VETERINARY JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
F. Vorimore, M. Hoelzer, E. M. Liebler-Tenorio, L-M Barf, S. Delannoy, M. Vittecoq, R. Wedlarski, A. Lecu, S. Scharf, Y. Blanchard, P. Fach, R. C. Hsia, P. M. Bavoil, R. Rossello-Mora, K. Laroucau, K. Sachse
Summary: The family Chlamydiaceae includes human and animal pathogens, a new Chlamydia strain was discovered in flamingos in 2011 and Chlamydiaceae-specific DNA was detected in more birds from 2012 to 2015, leading to the proposal of a new genus and two new species.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2021)
Correction
Biochemical Research Methods
Maximilian Collatz, Florian Mock, Emanuel Barth, Martin Hoelzer, Konrad Sachse, Manja Marz
Article
Infectious Diseases
Mughees Aizaz Alvi, Li Li, John Asekhaen Ohiolei, Warda Qamar, Muhammad Saqib, Muhammad Haleem Tayyab, Javaria Altaf, Khurram Ashfaq, Ali Hassan, Muhammad Jamal, Abdul Wahab, Anum Aizaz Alvi, Muhammad Usman, Muhammad Rashid Khalid Bajwa, Bao-Quan Fu, Hong-Bin Yan, Wan-Zhong Jia
Summary: The prevalence of H. taeniaeformis in urban rodent population in Pakistan is 22.09%, with higher infection rates in male and adult rats. Pakistani isolates of H. taeniaeformis are genetically closer to Asian and African population than those of European origin.
INFECTION GENETICS AND EVOLUTION
(2021)
Article
Infectious Diseases
Mughees Aizaz Alvi, Li Li, Muhammad Saqib, John Asekhaen Ohiolei, Muhammad Waqar Younas, Muhammad Haleem Tayyab, Ali Hassan, Warda Qamar, Anum Aizaz Alvi, Bao-Quan Fu, Hong-Bin Yan, Wan-Zhong Jia
Summary: This study conducted the first serosurvey in Pakistan to assess the exposure of butchers to Echinococcus granulosus, showing a high prevalence of cystic echinococcosis among butchers in the country. The results underscore the need for educating native slaughterhouse personnel on cystic echinococcosis and serve as a global warning, especially in developing countries.
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
(2021)
Review
Public, Environmental & Occupational Health
Tariq Jamil, Aman Ullah Khan, Muhammad Saqib, Muhammad Hammad Hussain, Falk Melzer, Abdul Rehman, Muhammad Zubair Shabbir, Mumtaz Ali Khan, Shahzad Ali, Asim Shahzad, Iahtasham Khan, Mudassar Iqbal, Qudrat Ullah, Waqas Ahmad, Muhammad Khalid Mansoor, Heinrich Neubauer, Stefan Schwarz
Summary: Brucellosis is a bacterial zoonotic disease that affects many animal species and can be transmitted to humans via direct contact or via contaminated food. Despite being a serious health hazard, public health concern for brucellosis has been neglected in many countries. In countries like Pakistan, where brucellosis is endemic, the disease continues to be significant.
FRONTIERS IN PUBLIC HEALTH
(2021)
Article
Infectious Diseases
H. Singha, F. Vorimore, S. Saini, T. Deshayes, M. Saqib, B. N. Tripathi, K. Laroucau
Summary: The study sequenced 10 B. mallei strains isolated from India and identified four new markers for tracking and differentiating strains, which will contribute to the epidemiological monitoring of glanders infections, especially in endemic regions such as South Asia and the Middle East.
INFECTION GENETICS AND EVOLUTION
(2021)
Article
Agriculture, Dairy & Animal Science
Qudratullah, Ghulam Muhammad, Tariq Jamil, Imaad Rashid, Qudrat Ullah, Muhammad Saqib
Summary: The study evaluated the efficacy of a locally prepared hemorrhagic septicemia and mastitis combined vaccine, showing significant immunogenic effects in dairy cows and buffaloes, including higher antibody titers, lower somatic cell counts and mastitis severity scores, as well as higher milk yield. Challenge studies are still needed to further validate the results.
Article
Veterinary Sciences
Mahvish Maqbool, Muhammad Sohail Sajid, Zafar Iqbal, Muhammad Saqib
Summary: The current study aimed to explore the correlation between different risk factors and the infestation rate of hard ticks in livestock population in Faisalabad district. The results showed that season, age, sex, feeding system, housing system, type of farming, farm structure, and hygienic measures were significantly associated with tick infestation rate. The study findings provide valuable information for tick control in similar husbandry systems in the selected areas and other regions.
JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY
(2022)
Article
Microbiology
Hamidullah, Muhammad Saqib, Muhammad Sohail Sajid, Sabir Hussain, Hafiz Muhammad Rizwan, Khurram Ashfaq, Sadia Ghazanfer, Asif Ali Butt, Mahvish Maqbool, Sibtain Ahmad, Olivier Andre Sparagano
Summary: The study found a high prevalence of canine toxoplasmosis in the owned and stray dog populations in Faisalabad District, Punjab, Pakistan. Bulldogs and Bhakarwal dogs showed the highest prevalence among owned and stray dogs, respectively. Young and female dogs were more susceptible to toxoplasmosis.
Article
Pharmacology & Pharmacy
Sami Ullah Khan, Rizwan Aslam, Muhammad Ashraf, Sultan Ali, Muhammad Saqib, Muhammad Arshad Khattak, Umer Sadique Khattak, Haq Amanullah, Hastari Wuryastuty, R. Wasito, Aris Haryanto, Farman Ullah, Menggen Ma, Sardar Ali
Summary: This study aimed to determine the prevalence and antimicrobial susceptibility of Shigella species in diarrheal pediatric patients under 5 years of age. The findings showed that Shigella isolates were susceptible to Levofloxacin, Azithromycin, and Cefotaxime, but resistant to Lincomycin and Penicillin-G.
PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Microbiology
Aneela Javed, Saifullah Khan Niazi, Eijaz Ghani, Maham Yamin, Muhammad Saqib, Hussnain A. Janjua, Ali Zohaib
Summary: The report presents the coding-complete genome sequence of a SARS-CoV-2 isolate obtained from a nasopharyngeal swab from the first COVID-19 patient in Gilgit, Pakistan.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Zoology
Muhammad Awais, Waqas Ahmad, Akhtar Rasool Asif, Sayyed Aun Muhammad, Iahtasham Khan, Muhammad Amjad Ali, Muhammad Qaiser Riaz, Muhammad Saqib
Summary: The study demonstrated significant benefits of using honey and povidone iodine mixture for wound healing, including improved wound contraction rate, tensile strength, hydroxyproline content, and shorter healing time. It is suggested that the combination of honey and povidone iodine should be considered for future wound treatment instead of using honey or povidone alone.
PAKISTAN JOURNAL OF ZOOLOGY
(2021)
