Article
Pharmacology & Pharmacy
Chaiwat Monmai, Jin-Suk Kim, Hyun Bo Sim, Doh-Won Yun, Sung-Dug Oh, Eui-Shik Rha, Jong-Jin Kim, So-Hyeon Baek
Summary: This study evaluated the effects of extracts from transgenic rice on inhibiting adipogenesis and anti-inflammatory activity. The results showed that the extracts significantly reduced lipid accumulation in cells and inhibited the expression of adipogenesis-related mRNA and inflammation-related factors. Therefore, transgenic rice may have potential as an obesity-reducing and/or -preventing agent.
Article
Biology
Catarina Teixeira, Andre P. Sousa, Ines Santos, Ana Catarina Rocha, Ines Alencastre, Ana Claudia Pereira, Daniela Martins-Mendes, Pedro Barata, Pilar Baylina, Ruben Fernandes
Summary: This study demonstrates that pioglitazone enhances adipocyte differentiation and secretome production, making it an excellent model for studying adiposity. The study also proposes an improved method for adipocyte secretome production, which can be used for experimental biology studies to understand the molecular mechanisms underlying the influence of adiposity on other cells.
Article
Biochemistry & Molecular Biology
Yuankuan Li, Sung Ho Lee, Meiyu Piao, Hyung Sik Kim, Kwang Youl Lee
Summary: This study found that Metallothionein 3 (MT3) plays an important role in the differentiation of 3T3-L1 cells into adipocytes. Overexpression of MT3 inhibited adipocyte differentiation and reduced the levels of adipogenic transcription factors C/EBPa and PPAR?. Further analysis revealed that MT3 suppressed the transcriptional activity of PPAR?, and this effect was not mediated by a direct interaction between MT3 and PPAR?. Additionally, MT3 overexpression decreased reactive oxygen species (ROS) levels during early adipocyte differentiation, while treatment with antimycin A, which induces ROS generation, restored the ROS levels.
Article
Engineering, Environmental
Yaqiang Cao, Yuanyuan Chen, Ke Miao, Shuyi Zhang, Fuchang Deng, Mu Zhu, Chao Wang, Wen Gu, Yixuan Huang, Zijin Shao, Xiaoyan Dong, Yufeng Gong, Hui Peng, Hui Yang, Yi Wan, Xudong Jia, Song Tang
Summary: Mounting evidence shows the close association between ambient PM2.5 exposure and obesity development, with adipose tissue being an important target. This study employed a preadipocyte differentiation model to comprehensively explore the adipogenic potential of PM2.5. PM2.5 exposure significantly increased fatty acid uptake, lipid accumulation, and adipogenic differentiation in a concentration-dependent manner. Transcriptome and lipidome analyses revealed disruption of transcriptional and lipid profiling at 10 μg/mL PM2.5. Activation of the peroxisome proliferator-activated receptor (PPAR) pathway and metabolism of glycerophospholipids, glycerolipids, and sphingolipids were most affected during adipocyte differentiation. The study demonstrated that PM2.5 promoted adipogenesis by activating PPAR. through reporter gene assays. The findings provide insights into PM2.5-induced adipogenesis and potential biomolecular targets for preventing PM2.5-induced obesity and related metabolic diseases.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Dandan Zhao, Yanyun Pan, Na Yu, Ying Bai, Rufeng Ma, Fangfang Mo, Jiacheng Zuo, Beibei Chen, Qiangqiang Jia, Dongwei Zhang, Jiaxian Liu, Guanjian Jiang, Sihua Gao
Summary: Accumulating evidence suggests that curcumin promotes the adipogenic differentiation of preadipocytes and enhances mitochondrial oxygen consumption in 3T3-L1 mature adipocytes by regulating the expression of UCP1, PRDM16, PPAR gamma, and PGC-1 alpha.
ROYAL SOCIETY OPEN SCIENCE
(2021)
Article
Integrative & Complementary Medicine
Eunbi Koh, Boram Kim, Kyungoh Choi
Summary: The study found that Torreya nucifera seed oil (TNSO) can promote adipocyte differentiation, increase intracellular lipid accumulation, and improve adipocyte function by activating adipogenic gene expression and PPAR gamma, STAT3 pathways.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2021)
Article
Nutrition & Dietetics
Elisabetta Melloni, Silvia Grassilli, Arianna Romani, Erika Rimondi, Annalisa Marcuzzi, Enrico Zauli, Paola Secchiero, Guglielmo Paganetto, Alessandra Guerrini, Gianni Sacchetti, Massimo Tacchini
Summary: Convolvulus pluricaulis is a commonly used Indian herb in Ayurvedic medicine known for its neuroprotective and neuroinflammatory action. Recent studies have shown its involvement in lipidic and glucidic metabolism, particularly in the control of hyperlipidaemia and diabetic conditions. It has been found to promote adipocyte differentiation and regulate glucose metabolism through the modulation of key regulators such as PPAR gamma and GLUT-4. Additionally, it exhibits anti-inflammatory effects by downregulating cytokines associated with insulin resistance and glucose intolerance. These findings suggest that Convolvulus pluricaulis could have potential benefits in glycemia and may be used as an adjunctive treatment for diabetes or for better glucose control.
Article
Biology
Amir Roshanzadeh, Anil Kumar Yadav, Sai-Prasad Pydi, Takefumi Kimura, Byeong-Churl Jang
Summary: This study demonstrates that the expression of beta 3-adrenergic receptor (beta 3-AR) is up-regulated during differentiation of 3T3-L1 cells in a p38 MAPK and PKC-dependent manner, and plays a crucial role in lipid accumulation.
Article
Endocrinology & Metabolism
Juu-Chin Lu, Chia-Yun Lu, Ying-Yu Wu
Summary: The depletion of THRAP3 exacerbated the pro-inflammatory response in adipocytes, attenuated the anti-inflammatory effects of TZD drugs, and greatly reduced the expression of PPAR gamma in differentiated adipocytes.
JOURNAL OF MOLECULAR ENDOCRINOLOGY
(2021)
Article
Cell Biology
Xin Lv, Jun Qiu, Tao Hao, Haoran Zhang, Haiping Jiang, Yang Tan
Summary: The study demonstrated that TSA inhibited adipogenesis in 3T3-L1 preadipocytes by activating the AMPK pathway, leading to decreased lipid accumulation. Furthermore, TSA was also able to reverse the weight gain and adipose tissue increase induced by a high-fat diet in obese mouse models.
Article
Biochemistry & Molecular Biology
Chaiwat Monmai, Jin-Suk Kim, So-Hyeon Baek
Summary: This study investigated the antiadipogenic and anti-inflammatory activities of resveratrol-enriched rice in 3T3-L1 adipocyte cells. It was found that resveratrol-enriched rice suppressed adipocyte differentiation and reduced the expression of adipogenic genes and signaling pathways. Additionally, it exhibited anti-inflammatory effects. These findings suggest the potential of resveratrol-enriched rice as an alternative obesity-reducing and anti-inflammatory agent.
Article
Biotechnology & Applied Microbiology
Eleanor H. Oates, Maciek R. Antoniewicz
Summary: Hypoxia has a significant impact on the physiology and metabolism of adipocytes, leading to the suppression of adipogenesis, de novo lipogenesis, and metabolic reprogramming. It alters the patterns of cellular metabolism, redirects glucose metabolism, rewires glutamine metabolism, suppresses branched chain amino acid consumption, and reduces the biosynthesis of certain fatty acids.
METABOLIC ENGINEERING
(2023)
Article
Biology
Nivethasri Lakshmana Perumal, Amila Mufida, Anil Kumar Yadav, Dae-Gu Son, Young-Wook Ryoo, Sung-Ae Kim, Byeong-Churl Jang
Summary: This study found that the inhibitor of p38 MAPK, TAK-715, could significantly inhibit the differentiation of preadipocytes and reduce lipid accumulation. TAK-715 exerts anti-adipogenic effects on 3T3-L1 cells and human adipose stem cells (hASCs) by regulating the expression and phosphorylation of p38 MAPK, C/EBP-alpha, PPAR-gamma, STAT-3, FAS, and perilipin A.
Article
Food Science & Technology
Na Gyeong Geum, Ho Jun Son, Joo Ho Yeo, Ju Hyeong Yu, Min Yeong Choi, Jae Won Lee, Jueng Kyu Baek, Jin Boo Jeong
Summary: HMR inhibited lipid accumulation in 3T3-L1 cells by suppressing the expression of proteins related to lipid accumulation and inducing proteasomal degradation of CEBP alpha via JNK and GSK3 beta activation.
FOOD SCIENCE & NUTRITION
(2021)
Article
Biochemistry & Molecular Biology
Palaniselvam Kuppusamy, Soundharrajan Ilavenil, In Ho Hwang, Dahye Kim, Ki Choon Choi
Summary: The study found that ferulic acid (FA) can significantly inhibit adipogenesis and lipid accumulation, enhance lipolysis by regulating key adipocyte factors and enzymes, and decrease adipocyte differentiation markers, showing potential as a potent molecule to prevent obesity and associated metabolic changes in the future.
Article
Cell Biology
Yingchun Zhu, Fangfang Zha, Bo Tang, Ting-Ting Ji, Xiao-Ying Li, Linhong Feng, Shou-Jun Bai
Summary: The study found that high glucose-induced circ_0125310 promotes the progression of diabetic nephropathy by regulating miR-422a and the IGF1R/p38 axis.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Hua Zhang, Lin Zhao, Jingjing Jiang, Jie Zheng, Li Yang, Yanyan Li, Jian Zhou, Tianshu Liu, Jianmin Xu, Wenhui Lou, Weige Yang, Lijie Tan, Weiren Liu, Yiyi Yu, Meiling Ji, Yaolin Xu, Yan Lu, Xiaomu Li, Zhen Liu, Rong Tian, Cheng Hu, Shumang Zhang, Qinsheng Hu, Yangdong Deng, Hao Ying, Sheng Zhong, Xingdong Zhang, Yunbing Wang, Hua Wang, Jingwei Bai, Xiaoying Li, Xiangfeng Duan
Summary: As cancer is increasingly seen as a metabolic disorder, serum metabolite profiling is proposed as a viable method for detecting cancer. In this study, a machine learning model using mass spectrometry-based liquid biopsy data is reported for pan-cancer screening and classification. The Multiplexed Nanomaterial-Assisted LDI for Cancer Identification (MNALCI) achieves high sensitivity and specificity for distinguishing cancers from healthy controls and accurately identifying the tissue of origin of tumors. This high throughput assay shows promising potential for non-invasive cancer diagnosis.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Jifeng Yang, Yaping Zhi, Shiyi Wen, Xuya Pan, Heting Wang, Xuemin He, Yan Lu, Yanhua Zhu, Yanming Chen, Guojun Shi
Summary: Dysregulated production of peptide hormones can lead to various endocrine diseases. Endoplasmic reticulum associated degradation (ERAD) plays a crucial role in maintaining ER proteostasis by degrading misfolded proteins. Targeting ERAD for regulating peptide hormone production is limited, and herbal products provide potential alternative therapeutics. In this study, a virtual screening was performed to identify compounds targeting SEL1L-HRD1 ERAD machinery, and Cryptochlorogenic acid (CCA) was found to enhance ERAD activity and promote the secretion of well-folded peptide hormone. These findings contribute to drug discovery strategies for targeting ER protein homeostasis and provide candidate compounds for hormone-related diseases.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Review
Materials Science, Multidisciplinary
Huanyu Li, Marco Liebscher, Dong Zhao, Bo Yin, Yuhang Du, Jian Yang, Michael Kaliske, Viktor Mechtcherine
Summary: Insufficient bonding between hydrophobic carbon fibers and water-based suspensions has limited the application of carbon fiber reinforced cements and other mineral building materials. Researchers have explored physical and chemical modification methods to enhance load transfer from mineral matrices to carbon fibers. Polymer impregnations and temperature-stable mineral suspensions have been used to improve the interface between carbon fibers and matrices, achieving better pullout behavior. Surface modification techniques such as oxidation and electrophoretic deposition have also been employed to improve fiber-matrix interactions. This article systematically reviews and compares these approaches, providing recommendations for achieving tailor-made interfacial bonding behavior.
PROGRESS IN MATERIALS SCIENCE
(2023)
Article
Engineering, Civil
Xing-er Wang, Armin Yousefi Kanani, Kai Pang, Jian Yang, Jianqiao Ye, Xiaonan Hou
Summary: This work developed a novel calibration strategy based on discrete element (DE) models and genetic expression programming (GEP) approach for predicting the behaviours of hybrid composite joints. The calibrated DE model can adaptively simulate the featured behaviours of both the ductile adhesive and composite joints with different configurations well in most examined occasions.
THIN-WALLED STRUCTURES
(2022)
Article
Pharmacology & Pharmacy
Rong Gao, Heting Wang, Ting Li, Jin Wang, Zhitao Ren, Nan Cai, Heying Ai, Shasha Li, Yan Lu, Yanhua Zhu, Xintao Shuai, Xuemin He, Guojun Shi, Yanming Chen
Summary: This study identifies major urinary proteins (MUPs), specifically MUP1, as novel proteins involved in regulating the endoplasmic reticulum (ER) stress response and potential therapeutic targets for non-alcoholic fatty liver disease (NAFLD). MUP1 is misfolded and trapped in the ER during ER stress, and recombinant MUP1 treatment alleviates ER stress response and improves insulin resistance in hepatocytes.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Cell Biology
Huo-Liang Zheng, Bo Li, Shao-Kuan Song, Peng-Bo Chen, Xin-Feng Zheng, Lei-Sheng Jiang, Sheng-Dan Jiang
Summary: This study evaluated the safety and effectiveness of using PEEK cages and cadaveric allografts in transforaminal lumbar interbody fusion (TLIF) for treating lumbar pyogenic spondylodiscitis. The results showed that this method is a safe and effective choice for treating lumbar pyogenic spondylodiscitis, achieving intervertebral fusion and restoring sagittal alignment without an increased risk of relapse.
MEDIATORS OF INFLAMMATION
(2023)
Article
Gastroenterology & Hepatology
Nan Cai, Xuanrong Chen, Jia Liu, Zheyao Wen, Siyin Wen, Wen Zeng, Shuo Lin, Yanming Chen, Guojun Shi, Longyi Zeng
Summary: This study aimed to investigate the effect of glucokinase (GK) activation on glucose and lipid metabolism in diet-induced obese mice. The results showed that GK activation improved glucose tolerance and insulin sensitivity while inducing hepatic lipid accumulation. Further studies revealed that GK activation activated the hepatic PERK-UPR signaling pathway through increasing lipogenic gene expression, leading to hepatic lipid accumulation.
Article
Construction & Building Technology
Mohammad Reza Ghaemdoust, Jian Yang, Feiliang Wang, Siping Li, Babak Jamhiri
Summary: The structural performance of concrete-filled steel tubes (CFSTs) is evaluated in this research, with the focus on enhancing serviceability through the application of tendons and modification of CFST configuration. Non-linear finite element and plastic stress distribution method are used for analysis, and sensitivity analysis and probabilistic assessment are conducted. The results demonstrate the significant improvement in moment capacity and serviceability performance of CFST beams with the implemented measures.
ADVANCES IN STRUCTURAL ENGINEERING
(2023)
Review
Construction & Building Technology
Lihui Li, Jian Yang, WangWei Liu, Pengfei Ren
Summary: This paper provides insights into selecting a suitable and reliable quantitative backscattered electron (QBSE) image analysis method for studying microstructure evolution in cement-based materials. It focuses on the technical principles, sample preparation, and the development of QBSE image analysis methods. The advantages and limitations associated with QBSE image analysis are also discussed.
CONSTRUCTION AND BUILDING MATERIALS
(2023)
Article
Gastroenterology & Hepatology
Bo Li, Ziyi Yang, Fei Mao, Wei Gong, Qing Su, Jialin Yang, Bin Liu, Yuping Song, Jie Jin, Yan Lu
Summary: This study reveals the crucial role of the miR-145a-5p/Nr4a2 regulatory axis in the progression from steatosis to NASH, suggesting that targeting this axis through miR-145a-5p supplementation or pharmacological inhibition of Nr4a2 in hepatocytes may be a promising therapeutic approach for NASH treatment.
JOURNAL OF HEPATOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Lihui Li, Jian Yang, Huanyu Li
Summary: This study presents a method based on EDS mapping analysis to accurately quantify the proportions and distributions of reaction products in alkaline-activated materials. The proposed method effectively removes noise and allows for clustering analysis, phase extraction, and component classification. By analyzing element ratios and distances, the chemical compositions and spatial distributions of various reaction products can be revealed, validating the reliability of this method.
MATERIALS & DESIGN
(2023)
Article
Endocrinology & Metabolism
Xiaoxin Xiang, Yanhua Zhu, Xuya Pan, Wei Xin, Jianning Chen, Weijian Tang, Ruomi Guo, Wu Yuan, Xuemin He, Li Zhou, Zhitao Ren, Siying Wen, Heting Wang, Yan Lu, Shasha Li, Tufeng Chen, Yuqi Zhou, Zulin Dou, Mengyin Cai, Xiaoyue Zhang, Yanming Chen, Guojun Shi
Summary: This study investigates the role of endoplasmic reticulum stress in mediating obesity-induced diaphragmatic dysfunction. The results show that people with obesity exhibit weakened diaphragmatic strength and lipid accumulation, which is also observed in obese mice. Inhibition of PERK/JNK signaling reverses lipid-induced impairment and elastic damage in C2C12 myotubes.
Article
Cell Biology
Heting Wang, Xuya Pan, Xiaoxin Xiang, Yang Zhang, Jianning Chen, Shiyi Wen, Jin Wang, Rong Gao, Jifeng Yang, Yaping Zhi, Siying Wen, Yubao Zheng, Ting Li, Heying Ai, Xuemin He, Yan Lu, Yanhua Zhu, Chunliang Li, Yanming Chen, Guojun Shi
Summary: By conducting a CRISPR screen, we found that RBBP8, an endonuclease involved in DNA damage repair, is necessary for ATF4 activation during ER stress. Deletion of RBBP8 led to impaired cell cycle progression, reduced proliferation, attenuated ATF4 activation, and lowered protein synthesis. RBBP8 was highly expressed in the liver and responsive to ER stress. In liver cancer tissues, both RBBP8 and ATF4 were highly expressed, and their expression correlated positively. Overexpression of RBBP8 promoted ATF4 activation under ER stress in vitro. These findings provide insights into how cells adapt to ER stress and how tumor cells survive in anticancer treatments, suggesting potential therapeutic strategies against liver disease.
CELL DEATH & DISEASE
(2023)
Article
Construction & Building Technology
Fangmu Chen, Jinxia Zhao, Baifa Zhang, Yuan Feng, Canguang Chen, Zhongyu Lu, Jian Yang, Jianhe Xie
Summary: Ultra-lightweight high-strength geopolymeric composites (ULHSGCs) were developed with the characteristics of being lightweight, high strength, and low carbon content. The study focused on examining the physical, mechanical, and microstructural properties of the ULHSGCs. The results showed that the ULHSGCs had good mechanical properties and fluidity.
CASE STUDIES IN CONSTRUCTION MATERIALS
(2023)
Article
Cell Biology
Karina A. Pena, Sofya Savransky, Breanna Lewis
Summary: Compartmentalization of GPCR signaling is an emerging topic that emphasizes the importance of spatial bias in signaling for physiological relevance. PTH1R was the first GPCR discovered to signal via cAMP from endosomes, challenging the conventional model of GPCR signaling. The location of cAMP generation determines the physiological outcomes of GPCR signaling.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Cheng-Xu Ma, Xiao-Ni Ma, Jin-Jin Liu, Cong-Hui Guan, Ying-Dong Li, Nan Zhao, Didac Mauricio, Song-Bo Fu
Summary: The downregulation of primary cilia (PCs) due to BRAFV600E mutation contributes to the aggressiveness and lymph node metastasis of Papillary Thyroid Cancer (PTC).
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Kejun Wu, Cuihua Huang, Wenrong Zheng, Yubin Wu, Qintao Huang, Menghua Lin, Ruonan Gao, Liqin Qi, Guanlian He, Xiaoying Liu, Xiaohong Liu, Linxi Wang, Zhou Chen, Libin Liu
Summary: Recurrent non-severe hypoglycemia in patients with diabetes is associated with cognitive impairment. This study found that this condition is associated with reduced mitophagy in the hippocampus, leading to mitochondrial dysfunction and neurological impairment.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Yifei Lv, Yizhou Huang, Huiyu Fan, Yunxiu Zhao, Linjuan Ma, Yibing Lan, Chunming Li, Peiqiong Chen, Zheng Lou, Jianhong Zhou
Summary: Before menopause, females have a lower incidence of cardiovascular disease than age-matched males, possibly due to the protective effects of sex hormones. 17 beta-E2 inhibits THBS1 expression, preventing cell senescence and apoptosis, and counteracts oxidative stress by suppressing the TGF-beta/Smad signaling pathway.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Ana C. L. Camargo, Flavia B. Constantino, Sergio A. A. Santos, Ketlin T. Colombelli, Luiz M. F. Portela, Matheus N. Fioretto, Luisa A. Barata, Guilherme T. Valente, Carlos S. Moreno, Luis A. Justulin
Summary: This study examined the effects of maternal malnutrition on the transcriptomic landscape of the ventral prostate in rats. It found that changes in molecular pathways related to cellular development and tissue morphogenesis were associated with maternal malnutrition. The Abcg1 gene was found to be deregulated in both malnourished rats and prostate cancer models and patients.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Sandra Romero-Cordoba, Mayel Chirinos, Nancy Noyola-Martinez, Nayeli Torres-Ramirez, Mitzi Garcia-Olivares, Juan Pablo Aragon-Hernandez, Ixchel Ramirez-Camacho, Rosa Zuniga, Fernando Larrea, Ali Halhali, David Barrera
Summary: This study analyzed the effects of calcitriol, TGF-131, and their combination on human trophoblast cells. The results showed that the combination treatment modified the transcriptional landscape and mainly affected the storage, activity, and metabolism of lipids, which may have an impact on placental development.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Traver J. Wright, Richard B. Pyles, Melinda Sheffield-Moore, Rachel R. Deer, Kathleen M. Randolph, Kristen A. Mcgovern, Christopher P. Danesi, Charles R. Gilkison, Weston W. Ward, Jayson A. Vargas, Peyton A. Armstrong, Sarah E. Lindsay, Mohammed F. Zaidan, Justin Seashore, Tamara L. Wexler, Brent E. Masel, Randall J. Urban
Summary: This study investigates the persistent neurologic symptoms in COVID-19 patients after recovery and explores the association between these symptoms and disrupted growth hormone secretion and gastrointestinal discomfort.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Li-Li Chen, Ya-Qin Li, Zhi-Hui Kang, Xuan Zhang, Su-Yan Gu, Na Wang, Xue-Yan Shen
Summary: Defects in trophoblastic EMT caused by dysregulation of circTNRC18's interaction with LIN28A play a vital role in the development of preeclampsia. LIN28A overexpression suppresses circTNRC18-mediated inhibition of trophoblast migration, invasion, and EMT, while LIN28A knockdown promotes them. Furthermore, circTNRC18 regulates the intracellular distribution of LIN28A and the expression of insulin-like growth factor II, affecting cell migration and invasion. Targeting the circTNRC18-LIN28A regulatory axis may provide a novel treatment approach for preeclampsia.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
