Journal
MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volume 319, Issue 1-2, Pages 109-115Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2010.01.004
Keywords
Purinergic; Progesterone; T47D-Y breast cancer cells; ATP
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Funding
- NICHD/NIH [1R03HD052770-01, NIDDK-2T32-DK-007012-26A1]
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Extracellular ATP activates purinergic (P-2) receptors with an increase in intracellular calcium and phosphorylation of MAPK. In this study we have investigated the effect of progesterone/progestin on ATP-induced calcium mobilization and phosphorylation of the kinase ERK in the T47D-Y breast cancer cell line that exhibits no detectable nuclear progesterone receptor expression Brief pretreatment with progesterone/progestin results in a dose dependent inhibition of ATP-induced intracellular calcium mobilization, and inhibition of ERK phosphorylation. Response to a cell impermeable ligand and inhibition of the response by an inactivating antibody suggests a mechanism of action at the plasma membrane These results in T47D-Y cells strongly suggest that progesterone can act in a rapid non-nuclear manner to inhibit extracellular ATP effects on intracellular calcium mobilization and ERK activation. This research provides an example of progesterone action in a breast cancer cell line lacking expression of the classical nuclear progesterone receptors (C) 2010 Elsevier Ireland Ltd All rights reserved
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