Article
Biochemistry & Molecular Biology
Martina Blaschke, Regine Koepp, Frank Streit, Johannes Beismann, Georg Manthey, Mark-Tilmann Seitz, Angelique Kragl, Heide Siggelkow
Summary: The enzyme 11 beta-HSD1 plays a key role in pre-receptor glucocorticoid metabolism, with increased expression leading to adipogenic differentiation in human mesenchymal progenitor cells. Inhibition experiments confirmed the enzyme's specificity for cortisol synthesis and adipogenic differentiation, potentially explaining the link between increased 11 beta-HSD1 expression, higher cortisol levels, and reduced bone quality in old age or in situations of supra-physiological glucocorticoid exposure.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Chemistry, Analytical
Yvonne Lood, Elisabeth Aardal, Johan Ahlner, Andreas Arlemalm, Bjorn Carlsson, Bertil Ekman, Jeanette Wahlberg, Martin Josefsson
Summary: The developed ESI-LC-MS/MS method for determination of testosterone in human serum and saliva is highly sensitive and accurate, with a wide applicability in clinical patients, healthy adults, and forensic cases.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Cailing Duan, Yan Wu, Jin Yang, Shenghuo Chen, Yun Pu, Huihua Deng
Summary: The study aimed to develop a sensitive method for simultaneous determination of illicit drugs and steroids in hair to monitor drug exposure and the health status of addicts. The validated method showed good linearity, quantification limits, and recoveries. The method successfully detected analytes in hair of female drug addicts with different drug abuse histories.
Article
Medical Laboratory Technology
Nils Backlund, Goran Brattsand, Staffan Lundstedt, Elisabeth Aardal, Inga Bartuseviciene, Katarina Berinder, Charlotte Hoybye, Pia Burman, Britt Eden Engstrom, Anders Isaksson, Anders Blomgren, Oskar Ragnarsson, Ulrika Ruetschi, Jeanette Wahlberg, Tommy Olsson, Per Dahlqvist
Summary: This study aimed to establish reference intervals for salivary cortisol and cortisone and evaluate their diagnostic accuracy for Cushing's syndrome. The results showed that multiple liquid chromatography-tandem mass spectrometry and immunoassay methods can be used to measure salivary cortisol and cortisone. The diagnostic accuracy for Cushing's syndrome was high, and all methods had high sensitivity and specificity.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Federico Ponzetto, Mirko Parasiliti-Caprino, Fabio Settanni, Antonello Nonnato, Giulio Mengozzi, Ezio Ghigo, Roberta Giordano
Summary: The simultaneous measurement of dexamethasone and cortisol has shown to improve the diagnostic performance of the overnight dexamethasone-suppression test. Additionally, monitoring the levels of corticosteroid drugs can help investigate unexpected changes in steroid hormone levels. This study developed and validated an LC-MS/MS method for quantifying cortisol, cortisone, dexamethasone, and other corticosteroids in serum/plasma samples. The method was successfully applied to patients undergoing the dexamethasone-suppression test and those receiving immunosuppressive treatment after kidney transplant.
Article
Biochemistry & Molecular Biology
Sarah Gregory, Scott G. G. Denham, Patricia Lee, Joanna P. P. Simpson, Natalie Z. M. Homer
Summary: This paper describes the application of an LC-MS/MS steroid analysis method to evaluate the reference ranges of steroids in saliva samples from older adults, and explores the correlation and sex differences of steroids in saliva.
Article
Endocrinology & Metabolism
Ru Zhang, German Rubinstein, Sharmilee Vetrivel, Sonja Kunz, Frederick Vogel, Lucas Bouys, Jerome Bertherat, Matthias Kroiss, Sinan Deniz, Andrea Osswald, Thomas Knoesel, Martin Bidlingmaier, Silviu Sbiera, Martin Reincke, Anna Riester
Summary: Steroid profiling by LC-MS/MS helped identify DHEA as a candidate reference hormone for cortisol secretion. Lateralization and different steroid ratios demonstrated the heterogeneity of steroid secretion in PBMAH patients. Patients with germline ARMC5 mutations exhibited dysregulation in the androgen pathway.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Endocrinology & Metabolism
Anna J. Anderson, Ruth Andrew, Natalie Z. M. Homer, Katherine A. Hughes, Luke D. Boyle, Mark Nixon, Fredrik Karpe, Roland H. Stimson, Brian R. Walker
Summary: Insulin upregulates cortisol regeneration in adipose tissue but not in skeletal muscle. In obesity, the balance between 11 beta-reductase and 11 beta-dehydrogenase activities likely leads to cortisol accumulation in adipose tissue, which may have adverse metabolic consequences.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Article
Endocrinology & Metabolism
Joseph J. Tonge, Brian G. Keevil, Jessica N. Craig, Martin J. Whitaker, Richard J. Ross, Charlotte J. Elder
Summary: The study compared different methods of salivary collection and found that Salivette, passive drool, and SalivaBio all yielded comparable concentrations of salivary cortisol and cortisone, which were stable under conditions replicating home collection. The SaliPac device was found to be an acceptable option for salivary sampling in young children.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Endocrinology & Metabolism
Lydia Johnson-Ferguson, Lilly Shanahan, Laura Bechtiger, Annekatrin Steinhoff, Josua Zimmermann, Markus R. Baumgartner, Tina M. Binz, Manuel Eisner, Denis Ribeaud, Boris B. Quednow
Summary: This study aimed to investigate the effects of different psychoactive substances on steroid hormone levels in hair while considering relevant covariates. The study found that the use of cannabis and ecstasy should be taken into account in studies examining steroid hormones.
PSYCHONEUROENDOCRINOLOGY
(2023)
Article
Medical Laboratory Technology
Julie A. Ray, Amol O. Bajaj, Erik K. Trier, Lisa M. Johnson
Summary: Cushing syndrome (CS) is caused by an excess of glucocorticoids, leading to symptoms like obesity, moon facies, hirsutism, and reddish-purple stretch marks. Most cases of CS are caused by exogenous glucocorticoids, known as iatrogenic CS. Screening for CS caused by a tumor can be done by measuring cortisol in urine, and detection of prednisone and prednisolone is important in understanding the potential prevalence of iatrogenic CS in patients.
CLINICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Rongmei Huang, Yi Hong, Yike Wu, Weifeng Li, Wenlan Liu
Summary: We developed a new LC-MS/MS method for simultaneous determination of serum testosterone and free testosterone levels. The method is simple to perform and can be applied in clinical practice, providing important information for diagnosis, treatment, and monitoring of androgen-related diseases.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Agriculture, Dairy & Animal Science
S. J. Weaver, P. I. Hynd, C. R. Ralph, J. E. Hocking Edwards, C. L. Burnard, E. Narayan, A. J. Tilbrook
Summary: The study tested the changes in cortisol concentrations in blood, saliva, feces, and wool in sheep after administering cortisol, showing that an increase in glucocorticoids in sheep blood is reflected in saliva, feces, and wool, indicating that measures of glucocorticoids in these matrices may provide a retrospective indicator of chronic stress.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2021)
Review
Chemistry, Analytical
Tengfei Yuan
Summary: Analyzing steroids in different biological matrices helps gain insight into the status of steroids.
MICROCHEMICAL JOURNAL
(2022)
Article
Veterinary Sciences
Solene Meunier, Michael Groessl, Claudia Reusch, Felicitas Boretti, Nadja Sieber-Ruckstuhl
Summary: Different saliva collection methods were evaluated in this study to diagnose and monitor Cushing's syndrome in dogs. The ginger stimulation method significantly increased saliva production and cortisol concentration. The 30s-ginger method showed potential for home sampling and monitoring of dogs with Cushing's syndrome.
BMC VETERINARY RESEARCH
(2021)
Article
Cell Biology
Natalia Rakova, Kathrin Juettner, Anke Dahlmann, Agnes Schroeder, Peter Linz, Christoph Kopp, Manfred Rauh, Ulrike Goller, Luis Beck, Alexander Agureev, Galina Vassilieva, Liubov Lenkova, Bernd Johannes, Peter Wabel, Ulrich Moissl, Joerg Vienken, Rupert Gerzer, Kai-Uwe Eckardt, Dominik N. Mueller, Karl Kirsch, Boris Morukov, Friedrich C. Luft, Jens Titze
Article
Medical Laboratory Technology
Jakob Zierk, Farhad Arzideh, Rainer Haeckel, Wolfgang Rascher, Manfred Rauh, Markus Metzler
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2013)
Review
Biochemical Research Methods
Manfred Rauh
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2012)
Article
Biochemical Research Methods
Wei Gao, Tobias Stalder, Paul Foley, Manfred Rauh, Huihua Deng, Clemens Kirschbaum
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2013)
Article
Dermatology
Peter Krieg, Sabine Rosenberger, Silvia de Juanes, Susanne Latzko, Jin Hou, Angela Dick, Ulrich Kloz, Frank van der Hoeven, Ingrid Hausser, Irene Esposito, Manfred Rauh, Holm Schneider
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Therese Koal, Diane Schmiederer, Hai Pham-Tuan, Cornelia Roehring, Manfred Rauh
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2012)
Article
Biochemistry & Molecular Biology
Lutz Naehrlich, Helmuth-Guenther Doerr, Azadeh Bagheri-Behrouzi, Manfred Rauh
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
(2013)
Article
Endocrinology & Metabolism
Robert Miller, Franziska Plessow, Manfred Rauh, Michael Groeschl, Clemens Kirschbaum
PSYCHONEUROENDOCRINOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Fabian B. Fahlbusch, Matthias Ruebner, Wolfgang Rascher, Manfred Rauh
Article
Medicine, General & Internal
Stefanie Seitz, Manfred Rauh, Martin Gloeckler, Robert Cesnjevar, Sven Dittrich, Andreas M. E. Koch
SWISS MEDICAL WEEKLY
(2013)
Letter
Dermatology
Peter Krieg, Angela Dick, Susanne Latzko, Sabine Rosenberger, Jason Meyer, Debra Crumrine, Thomas Hielscher, Peter M. Elias, Manfred Rauh, Holm Schneider
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2020)
Article
Medicine, Research & Experimental
Julia Baier, Maximilian Gaensbauer, Claudia Giessler, Harald Arnold, Mercedes Muske, Ulrike Schleicher, Soeren Lukassen, Arif Ekici, Manfred Rauh, Christoph Daniel, Arndt Ha Rtmann, Benjamin Schmid, Philipp Tripal, Katja Dettmer, Peter J. Oefner, Raja Atreya, Stefan Wirtz, Christian Bogdan, Jochen Mattner
JOURNAL OF CLINICAL INVESTIGATION
(2020)
Article
Medical Laboratory Technology
Tatjana Ammer, Andre Schuetzenmeister, Hans-Ulrich Prokosch, Jakob Zierk, Christopher M. Rank, Manfred Rauh
Summary: This study developed a benchmarking suite called RIbench for evaluating and comparing the performance of indirect methods in estimating reference intervals. Through the evaluation and comparison of several modern indirect methods, the results showed that these methods outperformed the direct method when the pathological fraction was below 20% and the sample size exceeded 5000.
CLINICAL CHEMISTRY
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Rafael Heiss, Lina Tan, Sandy Schmidt, Adrian P. Regensburger, Franziska Ewert, Dilbar Mammadova, Adrian Buehler, Jens Vogel-Claussen, Andreas Voskrebenzev, Manfred Rauh, Oliver Rompel, Armin M. Nagel, Simon Levy, Sebastian Bickelhaupt, Matthias S. May, Michael Uder, Markus Metzler, Regina Trollmann, Joachim Woelfle, Alexandra L. Wagner, Ferdinand Knieling
Summary: This study used low-field-strength MRI to characterize the morphologic and functional changes in lung tissue of children and adolescents with long COVID. The results suggest that low-field-strength MRI can help identify persistent pulmonary manifestations in this population.
Article
Obstetrics & Gynecology
Akos Varnagy, Jozsef Bodis, Gabor L. Kovacs, Endre Sulyok, Manfred Rauh, Wolfgang Rascher
JOURNAL OF REPRODUCTIVE MEDICINE
(2013)
Article
Cell Biology
Karina A. Pena, Sofya Savransky, Breanna Lewis
Summary: Compartmentalization of GPCR signaling is an emerging topic that emphasizes the importance of spatial bias in signaling for physiological relevance. PTH1R was the first GPCR discovered to signal via cAMP from endosomes, challenging the conventional model of GPCR signaling. The location of cAMP generation determines the physiological outcomes of GPCR signaling.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Cheng-Xu Ma, Xiao-Ni Ma, Jin-Jin Liu, Cong-Hui Guan, Ying-Dong Li, Nan Zhao, Didac Mauricio, Song-Bo Fu
Summary: The downregulation of primary cilia (PCs) due to BRAFV600E mutation contributes to the aggressiveness and lymph node metastasis of Papillary Thyroid Cancer (PTC).
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Kejun Wu, Cuihua Huang, Wenrong Zheng, Yubin Wu, Qintao Huang, Menghua Lin, Ruonan Gao, Liqin Qi, Guanlian He, Xiaoying Liu, Xiaohong Liu, Linxi Wang, Zhou Chen, Libin Liu
Summary: Recurrent non-severe hypoglycemia in patients with diabetes is associated with cognitive impairment. This study found that this condition is associated with reduced mitophagy in the hippocampus, leading to mitochondrial dysfunction and neurological impairment.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Yifei Lv, Yizhou Huang, Huiyu Fan, Yunxiu Zhao, Linjuan Ma, Yibing Lan, Chunming Li, Peiqiong Chen, Zheng Lou, Jianhong Zhou
Summary: Before menopause, females have a lower incidence of cardiovascular disease than age-matched males, possibly due to the protective effects of sex hormones. 17 beta-E2 inhibits THBS1 expression, preventing cell senescence and apoptosis, and counteracts oxidative stress by suppressing the TGF-beta/Smad signaling pathway.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Ana C. L. Camargo, Flavia B. Constantino, Sergio A. A. Santos, Ketlin T. Colombelli, Luiz M. F. Portela, Matheus N. Fioretto, Luisa A. Barata, Guilherme T. Valente, Carlos S. Moreno, Luis A. Justulin
Summary: This study examined the effects of maternal malnutrition on the transcriptomic landscape of the ventral prostate in rats. It found that changes in molecular pathways related to cellular development and tissue morphogenesis were associated with maternal malnutrition. The Abcg1 gene was found to be deregulated in both malnourished rats and prostate cancer models and patients.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Sandra Romero-Cordoba, Mayel Chirinos, Nancy Noyola-Martinez, Nayeli Torres-Ramirez, Mitzi Garcia-Olivares, Juan Pablo Aragon-Hernandez, Ixchel Ramirez-Camacho, Rosa Zuniga, Fernando Larrea, Ali Halhali, David Barrera
Summary: This study analyzed the effects of calcitriol, TGF-131, and their combination on human trophoblast cells. The results showed that the combination treatment modified the transcriptional landscape and mainly affected the storage, activity, and metabolism of lipids, which may have an impact on placental development.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Traver J. Wright, Richard B. Pyles, Melinda Sheffield-Moore, Rachel R. Deer, Kathleen M. Randolph, Kristen A. Mcgovern, Christopher P. Danesi, Charles R. Gilkison, Weston W. Ward, Jayson A. Vargas, Peyton A. Armstrong, Sarah E. Lindsay, Mohammed F. Zaidan, Justin Seashore, Tamara L. Wexler, Brent E. Masel, Randall J. Urban
Summary: This study investigates the persistent neurologic symptoms in COVID-19 patients after recovery and explores the association between these symptoms and disrupted growth hormone secretion and gastrointestinal discomfort.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Li-Li Chen, Ya-Qin Li, Zhi-Hui Kang, Xuan Zhang, Su-Yan Gu, Na Wang, Xue-Yan Shen
Summary: Defects in trophoblastic EMT caused by dysregulation of circTNRC18's interaction with LIN28A play a vital role in the development of preeclampsia. LIN28A overexpression suppresses circTNRC18-mediated inhibition of trophoblast migration, invasion, and EMT, while LIN28A knockdown promotes them. Furthermore, circTNRC18 regulates the intracellular distribution of LIN28A and the expression of insulin-like growth factor II, affecting cell migration and invasion. Targeting the circTNRC18-LIN28A regulatory axis may provide a novel treatment approach for preeclampsia.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)