Article
Immunology
Jiawei Liu, Ning Fu, Zhenbang Yang, Ang Li, Hongjiao Wu, Ye Jin, Qinqin Song, Shanshan Ji, Hongxue Xu, Zhi Zhang, Xuemei Zhang
Summary: This study evaluates the expression of CD55 in colon cancer and investigates its regulation by transcription factors and miRNA. The results indicate that CD55 is regulated by TFCP2, NF-kappa B, miR-27a-3p, and several immune-related genes, which in turn affects the development of colon cancer.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, Research & Experimental
Ji-Cheng Huang, Zhan-Peng Yue, Hai-Fan Yu, Zhan-Qing Yang, Yu-Si Wang, Bin Guo
Summary: TAZ plays an important role in anti-inflammation by preventing microglia from producing proinflammatory cytokines. It interacts with Nrf2 to enhance antioxidant capacity and inhibit mitochondrial dysfunction, leading to a reduction in oxidative stress and the inflammatory response. Nrf2 acts as a downstream target of TAZ to repress NF-kappa B activation and improve the microglia-mediated inflammatory response.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Multidisciplinary Sciences
Jon Huyghe, Dario Priem, Lisette Van Hove, Barbara Gilbert, Juergen Fritsch, Yasuo Uchiyama, Esther Hoste, Geert van Loo, Mathieu J. M. Bertrand
Summary: Cell death induced by tumor necrosis factor (TNF) can be beneficial during infection by helping to mount proper immune responses. However, TNF-induced death can also drive a variety of inflammatory pathologies. This study identifies a checkpoint that prevents apoptosis by detoxifying the cytotoxic complex formed upon TNF sensing.
Article
Oncology
Thomas A. Burley, Emma Kennedy, Georgia Broad, Melanie Boyd, David Li, Timothy Woo, Christopher West, Eleni E. Ladikou, Iona Ashworth, Christopher Fegan, Rosalynd Johnston, Simon Mitchell, Simon P. Mackay, Andrea G. S. Pepper, Chris Pepper
Summary: In this study, the researchers evaluated the effects of an NF-kappa B inducing kinase (NIK) inhibitor, CW15337, on primary chronic lymphocytic leukemia (CLL) cells, CLL and multiple myeloma (MM) cell lines, and normal B- and T-lymphocytes. They found that CW15337 induced apoptosis and inhibited the non-canonical NF-kappa B signaling pathway, reversing BCL2 family-mediated resistance. The combination of CW15337 with fludarabine or ABT-199 showed synergistic cytotoxic effects.
Review
Biochemistry & Molecular Biology
Li Tao, Xiaomeng Ren, Wenhui Zhai, Zheng Chen
Summary: The non-canonical NF-kappa B signaling pathway plays a crucial role in liver diseases by regulating liver homeostasis and participating in various pathological processes. Small-molecule inhibitors targeting this pathway have shown potential in the treatment of liver injuries.
Article
Immunology
Yu Zhang, Dan Zhang, Tingting Meng, Peng Tian, Jianlin Chen, Anbang Liu, Yan Zheng, Guohai Su
Summary: Breast cancer is a common cancer among women worldwide, and the chemotherapy drug DOX has the potential to prolong survival but can cause cardiovascular toxicity. The study found that SGK1 plays an important role in DOX-induced cardiotoxicity, with upregulated expression accompanied by increased levels of inflammatory factors. Inhibition of SGK1 can suppress the production of inflammatory factors and apoptosis of cardiomyocytes, providing cardioprotective effects. In addition, targeting SGK1 can inhibit the proliferation of breast cancer cells. The study suggests that inhibiting SGK1 may be an effective treatment strategy for breast cancer that has both tumor-killing and cardioprotective functions.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Immunology
Xiaohe Li, Qing Liang, Shaoyan Gao, Qiuyan Jiang, Fangxia Zhang, Ruiqin Zhang, Hao Ruan, Shuangling Li, Jiaoyan Luan, Ruxia Deng, Honggang Zhou, Hui Huang, Cheng Yang
Summary: Lenalidomide has potential anti-fibrotic effects by suppressing inflammation-related signaling pathways, thereby reducing pulmonary fibrosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Oncology
Paula Gonzalez-Lopez, Marta Alvarez-Villarreal, Ruben Ruiz-Simon, Andrea R. Lopez-Pastor, Melina Vega de Ceniga, Leticia Esparza, Jose L. Martin-Ventura, Oscar Escribano, Almudena Gomez-Hernandez
Summary: MicroRNAs miR-15a-5p and miR-199a-3p are significantly decreased in aortic and carotid samples from atherosclerosis patients and mice. Overexpression of these miRNAs can reduce inflammation and progression of atherosclerosis. MiR-15a-5p may serve as a novel diagnostic biomarker for advanced atherosclerosis.
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Liangge He, Xue Yu, Yulin Zhao, Haoran Lin, Yong Zhang, Danqi Lu
Summary: In this study, Epinephelus coioides soluble TLR5 (TLR5S) was identified as a negative regulator of the NF-kappa B signaling pathway. TLR5S specifically responded to Vibrio flagellin and inhibited flagellin-induced activation of NF-kappa B signaling and cytokine expression. This discovery provides insights into the negative regulation of immune signaling and the restoration of homeostasis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Nanxin Ma, Jian Guo, Xiaolong Wu, Zhenzhong Liu, Tian Yao, Qian Zhao, Ben Li, Fengjie Tian, Xiaoyan Yan, Wenping Zhang, Yulan Qiu, Yi Gao
Summary: This article systematically reviews and analyzes the changes in TLR4 signaling pathway-related proteins induced by arsenic exposure through meta-analysis, providing important theoretical basis for understanding the toxicity of arsenic and the mechanism of immune system damage.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Kyung-Hye Roh, Yeojin Lee, Je-Hyun Yoon, Danbi Lee, Eunju Kim, Eunchong Park, In Young Lee, Tae Sung Kim, Hyun Kyu Song, Jaekyoon Shin, Dae-Sik Lim, Eui-Ju Choi
Summary: MST1/STK4 acts as a negative regulator of the TLR4-NF-kappa B signaling pathway, inhibiting TRAF6 autoubiquitination and downstream signaling, thereby limiting inflammation induced by LPS. The research also suggests that MST1/STK4 can regulate the production of proinflammatory cytokines in macrophages.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Oncology
Xin Liu, Shenglin Zhang, Yi Dong, Yunpeng Xie, Qingshan Li
Summary: Gliomas, the most common primary brain tumors in adults, present clinical challenges due to their infiltrative growth patterns and high relapse tendencies. This study identified SENP1 as an upregulated SUMOylation regulator in gliomas and demonstrated its association with poor prognoses. Mechanistically, SENP1 inhibited SIRT1 expression through de-SUMOylation, leading to the activation of NF-kappa B and promotion of glioma cell malignant phenotype. This suggests that SENP1-mediated de-SUMOylation of SIRT1 could have therapeutic implications for gliomas.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Neurosciences
Yu-Hua Hu, Bao-Hua Jiao, Cheng-Ye Wang, Jian-Liang Wu
Summary: RIP2 is involved in TMZ resistance through the regulation of the NF-κB/MGMT signaling pathway. Interference with NF-κB or MGMT activity may be a novel strategy for treating RIP2-positive TMZ-resistant glioma.
CNS NEUROSCIENCE & THERAPEUTICS
(2021)
Article
Biology
Dongyang Wang, Xiaojing Zheng, Lihong Chai, Junli Zhao, Jiuling Zhu, Yanqing Li, Peiyan Yang, Qinwen Mao, Haibin Xia
Summary: In this study, the role of FAM76B in regulating neuroinflammation was demonstrated for the first time. It was shown that FAM76B could regulate the NF-kappa B-mediated inflammatory pathway by affecting hnRNPA2B1 translocation. This provides new insights into the mechanism of inflammation regulation.
Article
Biochemistry & Molecular Biology
Aftab Shaukat, Irfan Shaukat, Shahid Ali Rajput, Rizwan Shukat, Sana Hanif, Imran Shaukat, Xinxin Zhang, Chao Chen, Xuyang Sun, Tingzhu Ye, Kaifeng Niu, Zhiqiu Yao, Shadab Shaukat, Muhammad Safdar, Mohamed Abdelrahman, Umair Riaz, Junwei Zhao, Xiaoying Gu, Liguo Yang
Summary: Endometritis is an inflammatory response in the uterus that affects conception rates and reproductive intervals in dairy cows. Ginsenoside Rb1 has shown anti-inflammatory properties in LPS-induced endometritis, potentially through modulation of TLR4-mediated NF-kappa B signaling pathway. This study highlights the therapeutic potential of GnRb1 in treating endometritis and provides a basis for further research in both human and animal health.
Article
Biochemistry & Molecular Biology
Artem Artemov, Svetlana Zhenilo, Daria Kaplun, Alexey Starshin, Alexey Sokolov, Alexander M. Mazur, Justyna Szpotan, Maciej Gawronski, Martyna Modrzejewska, Daniel Gackowski, Egor B. Prokhortchouk
Summary: DNA methylation plays a significant role in cancer progression and can be affected by various environmental conditions, such as hypoxia. In renal cancer cells, inactivation of the VHL gene under normoxic conditions induces global genome hypermethylation, which can be reversed by exogenous expression of wild-type VHL. The hypermethylation in VHL mutants is caused by transcriptional changes in MDH and L2HGDH genes leading to accumulation of 2-hydroxyglutarate, inhibiting DNA demethylation by TET enzymes.
Article
Medicine, General & Internal
Bogna Kozlowska, Barbara Sochanowicz, Leszek Kraj, Malgorzata Palusinska, Piotr Kolsut, Lukasz Szymanski, Slawomir Lewicki, Witold Smigielski, Marcin Kruszewski, Przemyslaw Leszek
Summary: This study investigated the changes in iron-related proteins in the myocardium of heart failure patients. The results showed reduced expression of several proteins responsible for iron transport and storage, along with increased expression of oxidative stress markers. The reduction of iron-gathering proteins and elevated oxidative stress in failing hearts is detrimental to myocardial cells.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Chemistry, Physical
Kamil Brzoska, Maria Wojewodzka, Malgorzata Szczygiel, Agnieszka Drzal, Martyna Sniegocka, Dominika Michalczyk-Wetula, Eva Biela, Martyna Elas, Malgorzata Kucinska, Hanna Piotrowska-Kempisty, Lucyna Kapka-Skrzypczak, Marek Murias, Krystyna Urbanska, Marcin Kruszewski
Summary: This study analyzed the impact of different silver nanoparticles on the development and metastatic potential of breast cancer using a mouse model. The results showed that citrate-coated silver nanoparticles had a significant effect on reducing tumor volume and lung metastases in mice treated intragastrically. However, no effect on lung metastases was observed after intravenous administration of silver nanoparticles. The findings suggest that the anticancer activity of silver nanoparticles may depend on the activation of the immune system.
Review
Biology
Bogna Kozlowska, Barbara Sochanowicz, Leszek Kraj, Malgorzata Palusinska, Piotr Kolsut, Lukasz Szymanski, Slawomir Lewicki, Marcin Kruszewski, Marta Zaleska-Kociecka, Przemyslaw Leszek
Summary: Heart failure (HF) is a common disease and iron deficiency (ID) plays a significant role in worsening the condition of HF patients. Iron supplementation is recommended but may have adverse effects. There are still many knowledge gaps regarding the interaction between iron deficiency and HF. Summarizing the latest translational and clinical research on iron deficiency in HF is important for raising awareness among clinicians.
Article
Plant Sciences
Anna Kalinka, Marta Starczak, Daniel Gackowski, Edyta Stepien, Magdalena Achrem
Summary: 5-Hydroxymethylcytosine (5hmC) is found in the genomic DNA of rye species, and its quantity and chromosomal distribution suggest that it may play a role in regulating the rye genome.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Review
Biochemistry & Molecular Biology
Anna Lankoff, Malwina Czerwinska, Marcin Kruszewski
Summary: Prostate cancer is a common and deadly malignancy in men, with limited treatment options for advanced stages. Nanoparticles have been developed to selectively target cancer cells and improve imaging and therapy. This review focuses on the selection criteria and progress of nanoparticle-based radioconjugates for targeted imaging and therapy of prostate cancer.
Article
Chemistry, Physical
Kamil Brzoska, Barbara Sochanowicz, Malgorzata Szczygiel, Agnieszka Drzal, Martyna Sniegocka, Dominika Michalczyk-Wetula, Martyna Elas, Lucyna Kapka-Skrzypczak, Marcin Kruszewski
Summary: The epigenetic effects induced by citrate- and PEG-coated silver nanoparticles (AgNPs) in a 4T1 breast cancer mouse model were analyzed. It was found that both types of AgNPs significantly decreased the level of 5-methylcytosine (5-mC) in tumor tissue, with the effect being more pronounced for citrate-coated AgNPs. PEG-coated AgNPs also caused a significant decrease in DNA methylation after intravenous administration, and both types of AgNPs led to a decrease in histone H3 methylation.
Review
Plant Sciences
Dolores Rita Agius, Aliki Kapazoglou, Evangelia Avramidou, Miroslav Baranek, Elena Carneros, Elena Caro, Stefano Castiglione, Angela Cicatelli, Aleksandra Radanovic, Jean-Paul Ebejer, Daniel Gackowski, Francesco Guarino, Andrea Gulyas, Norbert Hidvegi, Hans Hoenicka, Vera Inacio, Frank Johannes, Erna Karalija, Michal Lieberman-Lazarovich, Federico Martinelli, Stephane Maury, Velimir Mladenov, Leonor Morais-Cecilio, Ales Pecinka, Eleni Tani, Pilar S. Testillano, Dimitar Todorov, Luis Valledor, Valya Vassileva
Summary: Epigenetic modifications, particularly DNA methylation, play a crucial role in preserving genome integrity and regulating gene expression, impacting growth, development, and stress response in plants. Detecting DNA methylation marks is essential for understanding these processes and enhancing crop productivity and stress resistance. This review provides an overview of different methods for detecting plant DNA methylation and compares their efficacy between model and crop plants. It also highlights the strengths and limitations of each method and emphasizes the importance of considering technical and biological factors. Overall, this review will assist scientists in selecting an appropriate DNA methylation profiling method.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Chemistry, Analytical
Rafal Rozalski, Fabian Lesniewski, Patrycja Baginska, Daniel Gackowski
Summary: This review article focuses on the recent developments and advances in the quantification of deoxynucleosides in clinical applications using liquid chromatography coupled with mass spectrometry. It discusses the use of stable isotopes as internal standards, the increasing automation in sample preparation and analysis, as well as the potential sources and importance of contamination in this type of analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Cell Biology
Jolanta Guz, Ewelina Zarakowska, Pawel Mijewski, Aleksandra Wasilow, Justyna Szpotan, Marek Foksinski, Bartosz Brzoszczyk, Daniel Gackowski, Piotr Jarzemski, Ryszard Olinski
Summary: The study found that the level of vitamin C in prostate tissues is exceptionally high, and the DNA epigenetic patterns are similar to other soft tissues but with unique characteristics. The findings suggest that prostatic vitamin C and DNA modifications may play an important role in maintaining prostate health.
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Magdalena Czajka, Krzysztof Sawicki, Magdalena Matysiak-Kucharek, Marcin Kruszewski, Jacek Kurzepa, Paulina Wojtyla-Buciora, Lucyna Kapka-Skrzypczak
Summary: Chlorpyrifos, a representative of organophosphorus pesticides, has effects on the viability, proliferation, differentiation, and fatty acid uptake in 3T3-L1 cells. Exposure to chlorpyrifos decreases cell viability but increases it at low concentrations. It inhibits proliferation and differentiation of preadipocytes, reduces lipid accumulation, and down-regulates key transcription factors. In fully differentiated adipocytes, chlorpyrifos increases basal free fatty acid uptake. These findings suggest that chlorpyrifos may play a role in the development of obesity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Physical
Katarzyna Sikorska, Krzysztof Sawicki, Magdalena Czajka, Lucyna Kapka-Skrzypczak, Marcin Kruszewski, Kamil Brzoska
Summary: The interest in nanoparticles and their effects on living organisms has been increasing in recent decades. Non-metallic nanoparticles appear to be less toxic than metallic ones, leading to their growing use in medicine and industry. This review summarizes current knowledge of the in vitro and in vivo neurotoxicity of non-metallic nanoparticles, as well as the mechanisms associated with their positive or negative effects on the central nervous system (CNS).
Article
Nanoscience & Nanotechnology
Kinga Malinowska, Bozena Bukowska, Ireneusz Piwonski, Marek Foksinski, Aneta Kisielewska, Ewelina Zarakowska, Daniel Gackowski, Paulina Sicinska
Summary: This study investigated the genotoxic potential of non-functionalized polystyrene nanoparticles (PS-NPs) of different diameters on human peripheral blood mononuclear cells (PBMCs). The results showed that PS-NPs decreased the metabolic activity of PBMCs, increased DNA strand break formation, oxidized purines and pyrimidines, and increased 8-oxodG levels. The extent of genotoxic changes in PBMCs depended on the size of the particles and their zeta-potential value.
