Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 335, Issue 1-2, Pages 83-89Publisher
SPRINGER
DOI: 10.1007/s11010-009-0245-8
Keywords
JNK; siRNA; CTGF; TGF-beta; Cornea
Categories
Funding
- Education Ministry Fund of China [20070422081]
- Natural Science Foundation of Shandong Province, China [2007GG20002031]
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Transforming-growth factor-beta 1 (TGF-beta 1) plays an important role in fibrosis by inducing connective tissue growth factor (CTGF) expression. In cornea, however, signaling pathway in the process is unclear. In this paper, real-time RT-PCR and Western blot analysis were used to examine the expression and TGF-beta 1-induced upregulation of CTGF in telomerase-immortalized human cornea stroma fibroblast cells (THSFs). A detailed molecular mechanism for TGF-beta 1-mediated cell proliferation and collagen I production was then investigated by detecting the activation of JNK pathway in TGF-beta 1-stimulated THSFs and blocking the JNK pathway using JNK1/2 siRNA. Both TGF-beta 1 and CTGF clearly induced cell proliferation in THSFs which was inhibited by functional blocking CTGF by anti-CTGF antibody. Blocking JNK pathway by JNK1/2 siRNA significantly inhibited induction of CTGF by TGF-beta 1. Taken together, the data indicate that CTGF is critical to the fibrosis process in wounded human cornea, acting via upregulation and activation of JNK signaling pathway.
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