Article
Multidisciplinary Sciences
Ryan A. Herold, Raphael Reinbold, Christopher J. Schofield, Fraser A. Armstrong
Summary: Electrochemical studies reveal that nanoconfinement significantly increases the efficiency of enzyme-catalyzed cascade reactions. By using a nanoporous conducting indium tin oxide film and entrapping Isocitrate dehydrogenase 1 (IDH1), the complete electrochemical oxidation of isocitrate to 2-oxoglutarate is achieved using only the NADP(H) cofactor carried into the electrode pores. The results demonstrate the power of nanoconfinement in facilitating multistep enzyme catalysis and provide insights into the role of nicotinamide cofactors as redox carriers.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Plant Sciences
Francisco J. Corpas, Salvador Gonzalez-Gordo, Jose M. Palma
Summary: Nitric oxide (NO) and hydrogen sulfide (H2S) play key roles in plant cells by regulating protein functions through post-translational modifications, impacting cellular redox status. NADPH is essential for cell growth and development, participating in various biochemical pathways, while NADP-dependent enzymes help generate NADPH by oxidoreductases. These molecules contribute to diverse cellular processes and detoxification, emphasizing the importance of redox balance in plant cells.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Review
Pharmacology & Pharmacy
Giorgia Colombo, Edoardo Luigi Maria Gelardi, Federica Carolina Balestrero, Marianna Moro, Cristina Travelli, Armando A. Genazzani
Summary: Tumor cells modify their cellular metabolism in order to sustain uncontrolled proliferation, requiring adequate amounts of NAD and NADPH to support overexpressed enzymes. This review explores the rationale for targeting key enzymes that maintain the cellular NAD/NADPH pool in colorectal cancer and enzymes that consume or use NADP(H).
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hun-Dong Lee, Su-Kyoung Yoo, Ho-Seok Yoo, Chul-Ho Yun, Geun-Joong Kim
Summary: The enzymatic transformation of chemicals using NADPH-dependent hydroxylase has gained attention, but its industrial application is limited due to high cost. Enzymatic NADPH-regeneration systems are developed as alternatives and have potential in various fields. Two recombinant isocitrate dehydrogenases (IDHs) were compared and found to function well with NADPH consumption systems. These enzymes could be used as alternatives for NADPH regeneration and have potential as fusion partners with NADPH-dependent enzymes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Isabelle A. MacLean, Anchal Varma, Kenneth B. Storey
Summary: This study investigated the dynamic nature of NADP-IDH during hibernation, revealing increased substrate affinity and protein phosphorylation levels. These changes suggest the ability of the organism to reduce energy consumption and enhance antioxidant activity during hibernation.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Yan Qiao, Can Wang, Yin Zeng, Tairan Wang, Jingjing Qiao, Chenze Lu, Zhao Wang, Xiangxian Ying
Summary: The study successfully constructed a strain expressing a fusion enzyme that achieved efficient NADP(+) regeneration and the selective oxidation of various alpha, beta-unsaturated alcohols. The researchers also optimized reaction conditions and established a platform for synthesizing valuable alpha, beta-unsaturated aldehydes.
MICROBIAL CELL FACTORIES
(2021)
Article
Multidisciplinary Sciences
Maithily S. Nanadikar, Ana M. Vergel Leon, Jia Guo, Gijsbert J. van Belle, Aline Jatho, Elvina S. Philip, Astrid F. Brandner, Rainer A. Boeckmann, Runzhu Shi, Anke Zieseniss, Carla M. Siemssen, Katja Dettmer, Susanne Brodesser, Marlen Schmidtendorf, Jingyun Lee, Hanzhi Wu, Cristina M. Furdui, Soren Brandenburg, Joseph R. Burgoyne, Ivan Bogeski, Jan Riemer, Arpita Chowdhury, Peter Rehling, Tobias Bruegmann, Vsevolod V. Belousov, Dorthe M. Katschinski
Summary: The authors identify the gamma-subunit of IDH3 as a redox switch linking oxidative stress to impaired metabolism and heart function, providing new insights into the relationship between redox signaling and cardiac function. They demonstrate that increased endogenous production of H2O2 leads to reversible impairment of cardiac contractility and identify specific cysteine residues in IDH3 gamma as critical for H2O2-dependent regulation of IDH3 activity.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Na Liu, Miao-Miao Lin, Si-Si Huang, Zi-Qi Liu, Jun-Chao Wu, Zhong-Qin Liang, Zheng-Hong Qin, Yan Wang
Summary: NADPH as a coenzyme produces reduced glutathione and ROS, while excessive glutamate receptor activation leads to mitochondrial impairment. This study investigates the effects of NADPH and Mito-apocynin on KA-induced excitotoxicity and the mechanism behind it. The combined administration of NADPH and NOX inhibitors offers better neuroprotection by reducing ROS generation, restoring neurons and mitochondrial function through the autophagy pathway.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Daniel C. Moreira, Debora P. Paula, Marcelo Hermes-Lima
Summary: Insect diapause involves biochemical adaptations, including reduced oxidative capacity and increased enzymatic activities in preparation for oxidative stress. These changes occur early in diapausing animals, within the first 24 hours to 20 days of diapause, indicating a complex response to metabolic depression and stress resistance in tropical insects like C. lacinia.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Oncology
Rui Li, Mengying Ke, Mingming Qi, Zhenru Han, Yuhao Cao, Zhendong Deng, Jinjun Qian, Ye Yang, Chunyan Gu
Summary: This study investigates the role of G6PD in multiple myeloma (MM). It is found that G6PD enhances the generation of the anti-oxidant NADPH and decreases the production of reactive oxygen species (ROS), thereby promoting resistance to Dexa-induced apoptosis in MM cells. The canonical Wnt/beta-catenin signaling pathway is also found to be involved in regulating G6PD-induced drug resistance and cellular redox levels of ROS.
EXPERIMENTAL HEMATOLOGY & ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Junyan Sun, Ying Guo, Yihui Fan, Qian Wang, Qiuwan Zhang, Dongmei Lai
Summary: Studies have shown that psychosocial stress is associated with female infertility, and reduced expression of IDH1 due to stress can inhibit granulosa cell proliferation and accelerate senescence via ROS activated MAPK signaling pathways. Restoring IDH1 activity may be a beneficial therapeutic strategy for ovarian dysfunction.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Toxicology
Masahide Inoue, Koji Sakamoto, Atsushi Suzuki, Shinya Nakai, Akira Ando, Yukihiko Shiraki, Yoshio Nakahara, Mika Omura, Atsushi Enomoto, Ikuhiko Nakase, Makoto Sawada, Naozumi Hashimoto
Summary: Our study using a murine model demonstrated that the pulmonary toxicity of silica nanoparticles depended on their physico-chemical properties through distinct mechanisms. Cellular uptake of larger particles by macrophages decreased, while surface amine modification modulated endosomal reactive oxygen species (ROS) signaling via NADPH oxidase 2 (NOX2), both of which were involved in mitigating the immune response in macrophages and resulting lung injury.
PARTICLE AND FIBRE TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hayet Houmani, Ahmed Debez, Larisse de Freitas-Silva, Chedly Abdelly, Jose M. Palma, Francisco J. Corpas
Summary: Potassium is an essential element for plant growth and its deficiency can cause oxidative stress. However, plants can survive by increasing antioxidant and NADPH-generating systems to cope with this stress.
Article
Cell Biology
Daniel Simoes de Jesus, Paula Bargi-Souza, Vinicius Cruzat, Vijay Yechoor, Angelo Rafael Carpinelli, Rodrigo Antonio Peliciari-Garcia
Summary: The pancreatic beta cells circadian clock and NADPH oxidase (NOX) family play important roles in glucose metabolism. Absence of BMAL1 may trigger an increase in NOX2-derived reactive oxygen species (ROS) in pancreatic beta cells, leading to inhibition of insulin secretion.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Tiziana Pecchillo Cimmino, Rosario Ammendola, Fabio Cattaneo, Gabriella Esposito
Summary: Reactive oxygen species (ROS) are highly reactive molecules that can induce cytotoxicity or regulate cellular physiology. Among the ROS, the superoxide anion radical (O2 center dot-) plays a key role in redox signaling, generated by the NADPH oxidase (NOX) enzyme family and the mitochondrial electron transport chain. In this review, the authors discuss the emerging roles of NOX enzymes in modulating cancer cell metabolism and highlight their potential as therapeutic targets for rewiring tumor metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Alejandro E. Leroux, R. Luise Krauth-Siegel
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Lucila Giordana, Maximo Hernan Sosa, Alejandro E. Leroux, Elkin F. Rodas Mendoza, Patricia Petray, Cristina Nowicki
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
(2018)
Review
Oncology
Alejandro E. Leroux, Joerg O. Schulze, Ricardo M. Biondi
SEMINARS IN CANCER BIOLOGY
(2018)
Review
Biochemistry & Molecular Biology
R. Luise Krauth-Siegel, Alejandro E. Leroux
ANTIOXIDANTS & REDOX SIGNALING
(2012)
Article
Biochemistry & Molecular Biology
Stefanie Zimmermann, Mouhssin Oufir, Alejandro Leroux, R. Luise Krauth-Siegel, Katja Becker, Marcel Kaiser, Reto Brun, Matthias Hamburger, Michael Adams
BIOORGANIC & MEDICINAL CHEMISTRY
(2013)
Article
Microbiology
Alejandro E. Leroux, Dante A. Maugeri, Fred R. Opperdoes, Juan J. Cazzulo, Cristina Nowicki
FEMS MICROBIOLOGY LETTERS
(2011)
Article
Biochemistry & Molecular Biology
Alejandro E. Leroux, Jurgen R. Haanstra, Barbara M. Bakker, R. Luise Krauth-Siegel
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Microbiology
Rachel B. Currier, Kathrin Ulrich, Alejandro E. Leroux, Natalie Dirdjaja, Matias Deambrosi, Mariana Bonilla, Yasar Luqman Ahmed, Lorenz Adrian, Haike Antelmann, Ursula Jakob, Marcelo A. Comini, R. Luise Krauth-Siegel
Review
Biochemistry & Molecular Biology
Alejandro E. Leroux, Ricardo M. Biondi
TRENDS IN BIOCHEMICAL SCIENCES
(2020)
Review
Chemistry, Medicinal
Lissy Z. F. Gross, Mariana Sacerdoti, Albrecht Piiper, Stefan Zeuzem, Alejandro E. Leroux, Ricardo M. Biondi
Article
Biochemistry & Molecular Biology
Mariana Sacerdoti, Lissy Z. F. Gross, Andrew M. Riley, Karin Zehnder, Abhijeet Ghode, Sebastian Klinke, Ganesh Srinivasan Anand, Kristina Paris, Angelika Winkel, Amanda K. Herbrand, H. Yasmin Godage, Gyles E. Cozier, Evelyn Suss, Joerg O. Schulze, Daniel Pastor-Flores, Mariela Bollini, Maria Victoria Cappellari, Dmitri Svergun, Melissa A. Graewert, Pedro F. Aramendia, Alejandro E. Leroux, Barry V. L. Potter, Carlos J. Camacho, Ricardo M. Biondi
Summary: The activation of mammalian kinases requires the phosphorylation of hydrophobic motifs by PDK1. PDK1 exists in different conformations and has selective substrate specificity.
Review
Biochemistry & Molecular Biology
Alejandro E. Leroux, Ricardo M. Biondi
Summary: This review presents the molecular mechanisms of protein kinase PDK1 in phosphorylating different substrates, including the colocalization with Akt/PKB and the interaction between the hydrophobic motif of substrates and the PIF-pocket of PDK1. The distinct conformations of full-length PDK1 also influence the interaction and phosphorylation of substrates.
BIOCHEMICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Alejandro Leroux, Ximena Fleming-Canepa, Alejandro Aranda, Dante Maugeri, Juan J. Cazzulo, Marco A. Sanchez, Cristina Nowicki
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
(2006)
