Article
Biochemistry & Molecular Biology
Marcin Przybylski, Dominik Pruski, Sonja Millert-Kalinska, Monika Krzyzaniak, Mateusz de Mezer, Magdalena Frydrychowicz, Robert Jach, Jakub Zurawski
Summary: This study aims to explore the relationship between the expression patterns of HPV E4 markers and the presence of HPV major capsid protein (L1) in cervical tissues. The study used a novel HPV-encoded marker and an HPV antibody to analyze the expression in terminally differentiated epithelial cells. The results showed that patients with LSIL-CIN 1 and HSIL-CIN 2 diagnoses had lower levels of HPV major capsid protein (L1) compared to those without confirmed cervical lesions.
Article
Biotechnology & Applied Microbiology
Ziwen Zheng, Xiaorong Yang, Xinyu Yao, Ling Li
Summary: The study explored the prognostic value of HPV 16/18 genotyping and geminin mRNA quantification in predicting the progression of low-grade cervical squamous intraepithelial lesion (LSIL). Results showed that HPV 16/18 positivity rate and geminin mRNA levels can differentiate between high-grade and low-grade squamous intraepithelial lesions, suggesting a potential role of geminin in the progression of LSIL. Combining HPV genotyping and geminin mRNA qRT-PCR could improve clinical intervention efficacy for progressive LSIL.
Review
Oncology
Ray Ramirez, Kristen Donohue
Summary: Currently, there is limited literature on the screening, monitoring, and treatment of anal intraepithelial neoplasms (AIN), especially in high risk populations. This review aims to provide detailed information on the current monitoring and indications for treatment of these precursor lesions, in order to prevent progression to invasive cancer.
JOURNAL OF SURGICAL ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Aneta Popiel-Kopaczyk, Aleksandra Piotrowska, Patrycja Sputa-Grzegrzolka, Beata Smolarz, Hanna Romanowicz, Piotr Dziegiel, Marzenna Podhorska-Okolow, Christopher Kobierzycki
Summary: During the epithelial-mesenchymal transition (EMT) process, cells undergo changes in phenotype, which can be observed in the metastatic phase of neoplastic diseases like cervical cancer. This study aimed to determine the localization and associations of TWIST, SNAIL, and SLUG proteins, which are markers of EMT, in precancerous lesions and cervical cancer. The immunohistochemical (IHC) analysis showed higher expressions of EMT markers in precancerous lesions and cervical cancer compared to the control group. Additionally, the expression of TWIST, SNAIL, and SLUG varied significantly between different grades of cervical intraepithelial neoplasia and cervical cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Nikki B. Thuijs, Marc van Beurden, Annette H. Bruggink, Renske D. M. Steenbergen, Johannes Berkhof, Maaike C. G. Bleeker
Summary: The study found that the risk of vulvar squamous cell carcinoma (VSCC) is considered lower in high-grade squamous intraepithelial lesion (HSIL) compared to differentiated vulvar intraepithelial neoplasia (dVIN), but more research is needed for confirmation. The incidence of high-grade VIN and cumulative VSCC risk is higher in dVIN compared to HSIL, with independent risk factors including type of VIN, age, and presence of lichen sclerosis. Recognition and early identification of patients with high-risk VIN, particularly dVIN, is crucial.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Gastroenterology & Hepatology
M. M. Saraiva, L. Spindler, N. Fathallah, H. Beaussier, C. Mamma, M. Quesnee, T. Ribeiro, J. Afonso, M. Carvalho, R. Moura, P. Andrade, H. Cardoso, J. Adam, J. Ferreira, G. Macedo, V de Parades
Summary: A CNN-based system was developed to automatically detect and differentiate HSIL versus LSIL in HRA images. The system achieved an overall accuracy of 90.3%, with sensitivity, specificity, positive and negative predictive values of 91.4%, 89.7%, 80.9%, and 95.6%, respectively. The area under the curve was 0.97.
TECHNIQUES IN COLOPROCTOLOGY
(2022)
Article
Pathology
Fernanda Silva Medeiros, Fabiana Oliveira dos Santos Gomes, Larissa Albuquerque Paiva, Neila Caroline Henrique da Silva, Mauro Cesar da Silva, Maria Carolina Valenta Rygaard, Christina Alves Peixoto, Stefan Welkovic, Maria Luiza Bezerra Menezes, Andrej Cokan, George Tadeu Nunes Diniz, Eduardo Antonio Donadi, Norma Lucena-Silva
Summary: In this study, the discriminatory power of histological findings associated with p16 immunohistochemistry staining to classify LSIL and HSIL was assessed. The results showed that p16 nuclear labeling had the best sensitivity and specificity for distinguishing between LSIL with low p16 expression and HSIL with high p16 expression. It was suggested that a hierarchical analysis with histology at the first level, followed by labeling analysis in the nucleus and then in the cytoplasm, could increase the accuracy of HPV cervical lesion stratification.
EXPERIMENTAL AND MOLECULAR PATHOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xiaohan Yang, Xinyue Shen, Zhujun Li, Wencai Li, Ying Liu
Summary: The study found correlations between the severity of cervical lesions and DNA methylation levels, infiltrating T cell numbers, and chemokine expressions. As cervical lesions progress, the numbers of immune cells decrease gradually, along with the loss of 5hmC and the occurrence of immunosuppression.
Article
Obstetrics & Gynecology
Sarah G. Bell, Emily K. Kobernik, Hope K. Haefner, Kathryn C. Welch
Summary: The study found that patients with high-grade vulvar squamous intraepithelial lesions were older and reported higher rates of depression and anxiety compared to patients with low-grade lesions. There were no significant differences in demographics such as race/ethnicity, education, marital status, or household income between the two groups.
JOURNAL OF LOWER GENITAL TRACT DISEASE
(2021)
Article
Public, Environmental & Occupational Health
Yu-Qin Ding, Jie Yu, Run-Qiu Wang, Lin Sang
Summary: In this study, the clinical and epidemiological features of HR-HPV infection in patients with cervical intraepithelial lesions were analyzed. It was found that patients between the ages of 31 and 40 were more likely to have HSIL and the most common types of HPV infection were HR-HPV16, HR-HPV33, and HR-HPV52. Additionally, patients aged 30 years and above who tested positive for one of 12 types of HPV but negative for TCT were at increased risk for developing HSIL, emphasizing the importance of colposcopy for early detection and treatment.
Article
Cell Biology
Nuria Carreras-Dieguez, Adela Saco, Marta del Pino, Claudia Pumarola, Ricardo Lopez del Campo, Carolina Manzotti, Adriana Garcia, Lorena Marimon, Sherley Diaz-Mercedes, Pere Fuste, Maria Teresa Rodrigo-Calvo, Naiara Vega, Aureli Torne, Natalia Rakislova
Summary: VSCC with HSIL-like lesions, especially HPV-independent cases, have a higher recurrence rate and may require more intensive treatment strategies and closer surveillance.
Article
Public, Environmental & Occupational Health
Yong Zeng, Tao Jiang, Yahong Zheng, Jing Yang, Hua Wei, Cunjian Yi, Yan Liu, Keming Chen
Summary: This study aimed to determine the risk factors predicting residual lesions in a subsequent hysterectomy after cold knife conization (CKC) for high-grade squamous intraepithelial lesion (HSIL). The results showed that positive margin, glandular involvement, HPV16/18 infection, and multiple high-risk HPV infection were independent high-risk factors for residual lesions. The logistic regression model had an AUC of 0.78.
Review
Oncology
Ara Unanyan, Laura Pivazyan, Julia Davydova, Kamila Murvatova, Alyona Khrapkova, Roman Movsisyan, Anton Ishchenko, Anatoly Ishchenko
Summary: This systematic review and meta-analysis focused on evaluating the efficacy of photodynamic therapy in cervical epithelial neoplasia and early-stage cervical cancer. The study found that PDT is most effective in treating CIN 2 and when photosensitizer is administered intravenously. While PDT may contribute to regression in CIN (LSIL), further evidence and long-term follow-up are necessary for a definitive conclusion.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2021)
Article
Immunology
Chunlei Guo, Wenkui Dai, Qian Zhou, Liming Gui, Han Cai, Di Wu, Jun Hou, Changzhong Li, Shuaicheng Li, Hui Du, Ruifang Wu
Summary: The microbial community in the lower female genital tract is associated with the risk of genital infection. This study found significant changes in the microbial community when squamous intraepithelial lesion (SIL) developed into high-grade SIL (HSIL).
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Medicine, General & Internal
Gunther A. Rezniczek, Samira Ertan, Sadia Rehman, Clemens B. Tempfer
Summary: The study assessed the performance of Lugol's iodine test during colposcopy, showing moderate sensitivity and poor specificity for detecting LSIL/HSIL. However, it was found to change clinical management in 5% of cases, indicating potential utility in addition to acetic acid.
Article
Pathology
Juliana Mota Siqueira, Yoshitsugu Mitani, Camilla Oliveira Hoff, Flavia Bonini, Luana Guimaraes de Sousa, Mario L. Marques-Piubelli, Anurag Purushothaman, Mutsumi Mitani, Hui Dai, Shiaw-Yih Lin, Michael T. Spiotto, Ehab Y. Hanna, Daniel J. McGrail, Adel K. El-Naggar, Renata Ferrarotto
Summary: B7-H4 expression pattern varies among different types of salivary gland carcinomas, and high B7-H4 expression is associated with poor prognosis in adenoid cystic carcinoma.
Article
Pathology
Basile Tessier-Cloutier, Felix K. F. Kommoss, David L. Kolin, Kristyna Nemejcova, Dupreez Smith, Jennifer Pors, Colin J. R. Stewart, W. Glenn Mccluggage, William D. Foulkes, Andreas von Deimling, Martin Kobel, Cheng-Han Lee
Summary: This study provides a detailed analysis of the clinical, pathological, immunohistochemical, and molecular features of DDOC/UDOC. The majority of patients presented with extraovarian disease and had rapid disease progression resulting in high mortality rate.
Review
Pathology
Sophia J. Wagner, Christian Matek, Sayedali Shetab Boushehri, Melanie Boxberg, Lorenz Lamm, Ario Sada, Dominik J. E. Winter, Carsten Marr, Tingying Peng
Summary: Computational pathology research driven by deep learning faces challenges in reproducibility and reusability. Codebase with good documentation and robustness and generalizability of models are crucial. The reuse of computational pathology algorithms is limited, and their application in clinical settings is even rarer. This study evaluates 160 peer-reviewed articles, providing criteria for data and code availability and statistical analysis of results.
Article
Pathology
Andres M. Acosta, Lynette M. Sholl, Fiona Maclean, Chia-Sui Kao, Thomas M. Ulbright
Summary: This study assessed the clinicopathologic and genomic features of 14 cases of testicular sex cord-stromal tumors. The results showed that CTNNB1 mutations are rare in these tumors, and most of them have genomic alterations similar to testicular sex cord-stromal tumors with pure or predominant spindle cell components.
Article
Pathology
Toru Odate, Kaishi Satomi, Takashi Kubo, Yuko Matsushita, Toshihide Ueno, Akira Kurose, Kohei Shomori, Tokiko Nakai, Reiko Watanabe, Keiko Segawa, Shusa Ohshika, Naritomo Miyake, Sayaka Kudo, Tatsunori Shimoi, Eisuke Kobayashi, Motokiyo Komiyama, Seiichi Yoshimoto, Fumihiko Nakatani, Akira Kawai, Yasushi Yatabe, Shinji Kohsaka, Koichi Ichimura, Hitoshi Ichikawa, Akihiko Yoshida
Summary: Inflammatory rhabdomyoblastic tumors (IRMTs) are newly recognized skeletal muscle tumors with uncertain malignant potential. This study investigated 13 IRMTs using clinicopathologic, genetic, and epigenetic methods. The results showed specific histologic features and genetic mutations in these tumors, and most of them exhibited benign behavior.
Article
Pathology
Dale L. Davis, Adam C. Lechner, David B. Chapel, Jonathan C. Slack, Chrystalle Katte Carreon, Bradley J. Quade, Carlos Parra-Herran
Summary: The Amsterdam Consensus Statement introduced the term maternal vascular malperfusion (MVM) to classify a group of findings related to impaired maternal-placental circulation. The study found that features such as low placental weight, accelerated villous maturation, decidual arteriopathy, and infarcts are associated with adverse obstetrical outcomes, while the role of other features like distal villous hypoplasia, excess multinucleated trophoblast, and retroplacental hemorrhage needs further research.
Review
Pathology
Alain C. Borczuk
Summary: COVID-19 is an acute respiratory illness that can progress to acute respiratory distress syndrome. While most patients recover completely, some may experience persistent respiratory dysfunction, known as long COVID. The pathogenesis involves immune and cellular disturbances.
Article
Pathology
Annikka Weissferdt, Cheuk H. Leung, Heather Lin, Boris Sepesi, William N. William, Stephen G. Swisher, Tina Cascone, J. Jack Lee, Abujiang Pataer
Summary: Neoadjuvant treatment of non-small cell lung cancer challenges traditional processing of pathology specimens, and accurate evaluation of residual tumor is crucial for assessing treatment efficacy.