Article
Pharmacology & Pharmacy
Chien - Lin, Zan -Chu Chen, Chien -Hung Chen, Yun-Hsuan Chang, Tsai-Chia Lee, Tsai-Tai Tang, Tzu-Wei Yu, Chih-Man Yang, Ming-Chang Tsai, Chi-Chou Huang, Tzu-Wei Yang, Chun-Che Lin, Rou-Hsin Wang, Guang-Yuh Chiou, Yuh-Jyh Jong, Jui- Chao
Summary: In colorectal cancer patients, the levels of Haspin and phospho-H3T3 protein are highly expressed. Overexpression of Haspin increases the levels of p-H3T3 and survivin proteins in CRC cells. Knockdown of Aurora A gene leads to abnormal chromosome segregation, mitotic catastrophe and cell growth inhibition. Combined treatment of Aurora A and Haspin inhibitors enhances apoptosis and tumor inhibition in CRC.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Lucia Ramos-Alonso, Petter Holland, Stephanie Le Gras, Xu Zhao, Bernard Jost, Magnar Bjoras, Yves Barral, Jorrit M. Enserink, Pierre Chymkowitch
Summary: Mitotic entry is associated with a series of cellular changes in chromosomes and transcription factors, leading to downregulation of transcription. However, the influence of mitotic condensation on transcription during interphase is unknown. In this study, we found that failure to condense a specific chromosome during mitosis resulted in aberrant transcription in the subsequent interphase, leading to high expression levels of genes in cis and the activation of inducible transcriptional programs in trans. These findings reveal an unexpected role of mitosis and mitotic chromosome condensation in maintaining transcriptional homeostasis during interphase.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Tiansheng Zeng, Wenhui Huang, Longzhen Cui, Pei Zhu, Qing Lin, Wenjuan Zhang, Junyi Li, Cong Deng, Zhihua Wu, Zeyong Huang, Zhiyong Zhang, Tingting Qian, Wei Xie, Min Xiao, Yingyu Chen, Lin Fu
Summary: This study depicted the profile of elevated extrachromosomal circular DNA (eccDNA) in normal hematopoiesis and acute myeloid leukemia (AML) evolution. The number of eccDNAs was found to increase with the evolution of normal hematopoiesis and AML. AML-specific genes and recurrent eccDNAs were identified, which could serve as potential biomarkers for AML treatment.
CELL DEATH DISCOVERY
(2022)
Article
Biochemistry & Molecular Biology
Mei-Chih Chen, Bing-Ze Yang, Wei-Wen Kuo, Shih-Hsin Wu, Tso-Fu Wang, Yu-Lan Yeh, Ming-Cheng Chen, Chih-Yang Huang
Summary: Resistance to chemotherapy is a major obstacle in cancer treatment. This study investigated the molecular mechanisms underlying drug resistance in colon cancer using oxaliplatin-resistant LOVOOR cells. The findings suggest that Aurora-A, a kinase involved in the G2/M phase, may contribute to the resistance of LOVOOR cells to oxaliplatin treatment by depressing p53 signaling. Targeting Aurora-A could be a potential solution for patients who have failed oxaliplatin treatment.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Saya Ito, Takashi Ueda, Atsushi Yokoyama, Atsuko Fujihara, Fumiya Hongo, Osamu Ukimura
Summary: PCA3, a prostate cancer-specific lncRNA, promotes cancer progression by interacting with nucleoplasmic lamins via LAP2 alpha. Knockdown of PCA3 leads to changes in levels of HP1 gamma and histone modifications, as well as activation of the p53 signaling pathway and cell cycle arrest. These findings suggest a unique mechanism where PCA3 controls chromatin organization through regulation of nucleoplasmic lamins, potentially mediating cancer progression.
CANCER GENE THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Maximilian F. D. Spicer, Daniel W. Gerlich
Summary: Chromosomes undergo transformations during the cell cycle, including transcription, replication, and segregation. These changes are driven by DNA loop extrusion and a chromatin solubility phase transition. Condensins enrich at an axial core, forming loops and providing resistance to spindle pulling forces. Mitotic chromosomes are further compacted by deacetylation of histone tails, making them insoluble and resistant to microtubule penetration. Ki-67 regulates surface properties, allowing independent chromosome movement in early mitosis and clustering during mitotic exit. Recent progress has shed light on the material properties of chromatin and how they facilitate accurate chromosome segregation.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Joshua A. R. Brown, Jieying Hazel Cui, Maggie Y. M. Ling, Ellia X. C. Gao, LeAnn J. Howe, Sheila S. Teves, James Davie
Summary: The 43rd Asilomar Chromatin, Chromosomes, and Epigenetics Conference was held online, allowing researchers at different career stages to share promising new findings. The conference covered modern chromatin research in various model systems and explored the fundamental principles of nuclear organization and transcription regulation, as well as key mechanisms underlying human disease.
BIOCHEMISTRY AND CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Joshua A. R. Brown, Jieying Hazel Cui, Maggie Y. M. Ling, Ellia X. C. Gao, LeAnn J. Howe, Sheila S. Teves
Summary: The 43rd Asilomar Chromatin, Chromosomes, and Epigenetics Conference provided a platform for researchers at various career stages to share promising new findings in the field of chromatin research. The conference covered a wide range of topics, including nuclear organization, transcription regulation, and mechanisms underlying human diseases.
BIOCHEMISTRY AND CELL BIOLOGY
(2022)
Article
Oncology
Stefanie Schuller, Jan Sieker, Philip Riemenschneider, Bianca Koehler, Elisabeth Drucker, Sofia M. E. Weiler, Daniel Dauch, Carsten Sticht, Benjamin Goeppert, Stephanie Roessler, Silvia Ribback, Kai Breuhahn, Falko Fend, Frank Dombrowski, Kerstin Singer, Stephan Singer
Summary: The tumor suppressor protein P53 plays a crucial role in preventing liver cancer development, and it negatively regulates the expression of HELLS, an important regulator in liver cancer. The regulatory mechanism involves the induction of P21 and repression of FOXM1, resulting in the downregulation of HELLS expression. This study provides new insights into the epigenetic regulation of liver cancer and the tumor suppressive function of P53.
Review
Oncology
Minji Jo, Yoshiharu Kusano, Toru Hirota
Summary: This review outlines possible etiologies of chromosomal instability (CIN) in malignant tumors, focusing on key processes such as sister chromatid cohesion, kinetochore-microtubule attachment, bipolar spindle formation, spindle-assembly checkpoint, and separase activity, as well as potential chromosome structural issues during DNA replication. Understanding the molecular etiologies of CIN may lead to novel strategies to intervene in CIN for cancer control.
Article
Multidisciplinary Sciences
Michael R. Williams, Yan Xiaokang, Nathaniel A. Hathaway, Dmitri Kireev
Summary: This study used a simulation model to observe molecular self-organization leading to heterochromatin formation and provided a structural interpretation of important traits of the heterochromatinization process. The study also elucidated the time scale of repressive response to a heterochromatin-triggering event.
Article
Multidisciplinary Sciences
Matthew B. Couger, Scott W. Roy, Noelle Anderson, Landen Gozashti, Stacy Pirro, Lindsay S. Millward, Michelle Kim, Duncan Kilburn, Kelvin J. Liu, Todd M. Wilson, Clinton W. Epps, Laurie Dizney, Luis A. Ruedas, Polly Campbell
Summary: The sex chromosome system of the creeping vole is unique, with females having an unpaired X chromosome (X0) and the sex chromosome being one of the two X chromosomes, both carrying fragments of the ancestral Y chromosome. The consequences of this transformed sex chromosome system in this species include Y-like degeneration, expression of Y-linked genes in females, and X inactivation of the male-specific chromosome in male somatic cells.
Article
Multidisciplinary Sciences
Shi Pan, Maria Secrier
Summary: H&E stained slides are widely used in disease diagnosis, and deep learning enables the detection of complex molecular patterns for automated decision-making. HistoMIL is a Python package designed to streamline the implementation and training process of MIL-based algorithms, achieving up to 85% AUROC performance on breast cancer histology slides.
Review
Biochemistry & Molecular Biology
Alyssa Ialongo, Ssu-Yu Yeh, Ho Sung Rhee
Summary: The International Asilomar Chromatin, Chromosomes, and Epigenetics Conference was held online and focused on chromosome-related research topics. It featured invited speakers and provided a platform for scientists to exchange ideas and present their research findings.
BIOCHEMISTRY AND CELL BIOLOGY
(2023)
Article
Biology
Dmitrij Dedukh, Alla Krasikova
Summary: Genome stability is crucial for eukaryotic organisms, but some organisms can selectively eliminate part of their genomes during specific developmental stages. Programmed DNA elimination, occurring independently in different organisms, serves as a mechanism for dosage compensation and inactivation of unnecessary or dangerous genetic entities. Genome elimination in hybrids results from competition between parental genomes.
BIOLOGICAL REVIEWS
(2022)
Article
Cell Biology
Fang Yu, Xiaoqun Ba, Hanjin Yang, Ke Huang, Yafei Zhang, Han Zhang, Liming Xu, Jinghan Wang, Lijun Wang, Zhaoming Wang, Hong Fang, L. Jeffrey Medeiros, Wei Wang
Summary: Exuberant large T-cell proliferations in Kikuchi disease can be misdiagnosed as lymphoma, but a comprehensive analysis of clinical, pathological, and immunophenotypic features can help distinguish between the two.
Article
Pathology
Yi-Sian Lu, Po-Min Chiang, Yi-Ching Huang, Shiang-Jie Yang, Liang-Yi Hung, L. Jeffrey Medeiros, Ya-Ping Chen, Tsai-Yun Chen, Ming-Shi Chang, Kung-Chao Chang
Summary: Diffuse large B-cell lymphoma (DLBCL) is the most common type of lymphoma worldwide. This study found that interleukin-20 (IL-20) is frequently expressed in different types of lymphoma, especially in follicular lymphoma, Burkitt lymphoma, and mantle cell lymphoma. Overexpression of IL-20 promotes cell apoptosis in DLBCL.
Letter
Pathology
Tsung-Heng Tsai, Che-Wei Hsu, L. Jeffrey Medeiros, Kung-Chao Chang
Article
Pathology
Jie Xu, Shaoying Li, C. Cameron Yin, Keyur P. Patel, Guilin Tang, Wei Wang, Roberto N. Miranda, Sofia Garces, Zhenya Tang, Pei Lin, L. Jeffrey Medeiros
Summary: Granulomatous reactions can occur in patients with Hodgkin lymphoma and have distinct clinical and pathological features. These patients are predominantly male and usually over 40 years old. They commonly present with lymphadenopathy, particularly in the neck and supraclavicular areas. Advanced stage disease and B symptoms are frequently observed. The histiocytes in the granulomas express PD-L1 and pSTAT3, but not pERK and cyclin D1. The 5-year overall survival rate for these patients is 78%.
Article
Pathology
Zhihong Hu, L. Jeffrey Medeiros, Mina Xu, Ji Yuan, Deniz Peker, Lina Shao, Zhenya Tang, Brenda Mai, Beenu Thakral, Adan Rios, Shimin Hu, Wei Wang
Summary: This study aimed to investigate the clinicopathologic features and molecular profile of T-PLL associated with t(X;14)(q28;q11.2). The results showed that T-PLL patients with t(X;14)(q28;q11.2) frequently have lymphocytosis, morphological abnormalities in leukemic cells, complex karyotypes, and mutations involving JAK/STAT pathway. The overall survival rate was poor, with 8 out of 15 patients dying during the follow-up period.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2023)
Article
Pathology
Siba El Hussein, Kirill A. Lyapichev, Hong Fang, Fatima Zahra Jelloul, Warren Fiskus, Jiansong Chen, Peng Wei, Ellen Schlette, Jie Xu, Shaoying Li, Rashmi Kanagal-Shamanna, Hong Yang, Zhenya Tang, Beenu Thakral, Sanam Loghavi, Nitin Jain, Philip A. Thompson, Alessandra Ferrajoli, William G. Wierda, Elias Jabbour, Keyur P. Patel, Bouthaina S. Dabaja, Kapil N. Bhalla, Joseph D. Khoury
Summary: Integrated clinicopathological and molecular analyses were performed on 142 cases of RT-DLBCL. The study found that RT-DLBCL has distinctive morphological and immunophenotypic features, including immunoblastic morphology and common expression of CD5, MUM1, and LEF1. Cell-of-origin does not seem to have prognostic implications in RT-DLBCL.
Article
Pathology
Siba El Hussein, L. Jeffrey Medeiros, Stephen K. Gruschkus, Peng Wei, Ellen Schlette, Hong Fang, Fatima Zahra Jelloul, Wei Wang, Warren Fiskus, Rashmi Kanagal-Shamanna, Sanam Loghavi, Hong Yang, Shaoying Li, Jie Xu, Zhenya Tang, Beenu Thakral, Nitin Jain, William G. Wierda, Keyur Patel, Kapil N. Bhalla, Joseph D. Khoury
Summary: Immune checkpoint inhibitors (PD-1 inhibitors) have shown clinical activity in Richter transformation-diffuse large B-cell lymphoma variant (RT-DLBCL), providing a novel therapeutic approach. A study group of 64 RT-DLBCL patients was assessed for expression of PD-1, PD-L1, CD30, and microsatellite instability (MSI), as well as evaluated for immune evasion phenotype (IEP) and PD1-positive tumor-infiltrating lymphocytes (TILs). Brisk PD1+ TIL level was more common in IEP+ tumors, and CD30 expression was significantly more common in IEP+ RT-DLBCL. There was no significant difference in age, sex, or time to transformation between the two groups. Notably, patients with brisk PD1+ TILs had better overall survival.
Article
Oncology
Guilin Tang, Shaoying Li, Gokce A. Toruner, Preetesh Jain, Zhenya Tang, Shimin Hu, Jie Xu, Joanne Cheng, Melissa Robinson, Francisco Vega, L. Jeffrey Medeiros
Summary: FISH analysis using MYC break-apart probes is a commonly used technique to assess for MYC rearrangement. However, atypical signal patterns can occur, such as gain or loss of 5'MYC or 3'MYC. This study evaluated patients with aggressive B-cell lymphomas who exhibited atypical FISH signal patterns and found that 5'MYC gain is associated with MYC expression and a poorer prognosis, while 3'MYC deletions are not associated with MYC expression or a poorer prognosis and may be unrelated to MYC-R.
Article
Oncology
John Diks, Zhenya Tang, Mehmet Altan, Sarah Anderson, Hui Chen, Asif Rashid, Richard Kenneth Yang, Mark J. Routbort, Keyur P. Patel, Gokce A. Toruner, L. Jeffrey Medeiros, Guilin Tang, Rajyalakshmi Luthra, Sinchita Roy-Chowdhuri
Summary: This study evaluated the efficacy of RNA-based NGS assay in detecting genomic alterations in NSCLC cytology specimens and compared the results with FISH testing. The results showed that RNA-based NGS can accurately detect gene fusions in NSCLC cytology with high concordance with FISH results. However, RNA-based NGS may have high failure rates, suggesting the need for orthogonal testing for comprehensive genomic profiling.
CANCER CYTOPATHOLOGY
(2023)
Letter
Pathology
Yu-Ting Yu, Kuan-Yu Lin, Ya-Ping Chen, Yi-Lin Chen, L. Jeffrey Medeiros, Kung-Chao Chang
Meeting Abstract
Medicine, Research & Experimental
Do Hwan Kim, Fatima Zahra Jelloul, Sanam Loghavi, Rashmi Kanagal-Shamanna, C. Cameron Yin, Zhuang Zuo, Keyur Patel, Mark Routbort, Shaoying Li, Francisco Vega, Roberto Miranda, L. Jeffrey Medeiros, Chi Young Ok
LABORATORY INVESTIGATION
(2023)
Meeting Abstract
Medicine, Research & Experimental
Do Hwan Kim, Guilin Tang, Pei Lin, Jie Xu, Sa Wang, Lianqun Qiu, C. Cameron Yin, Wei Wang, L. Jeffrey Medeiros, Shaoying Li
LABORATORY INVESTIGATION
(2023)
Meeting Abstract
Medicine, Research & Experimental
Meng Liu, Xiaoqiong Wang, E. Shuyu, Guilin Tang, Wei Wang, Fatima Zahra Jelloul, Pei Lin, L. Jeffrey Medeiros, Shimin Hu
LABORATORY INVESTIGATION
(2023)
Meeting Abstract
Medicine, Research & Experimental
Karen Nahmod, Beenu Thakral, Wei Wang, Fnu Aakash, Keyur Patel, Mark Routbort, Rashmi Kanagal-Shamanna, Chi Young Ok, Guilin Tang, Francisco Vega, L. Jeffrey Medeiros, Sa Wang, Sanam Loghavi
LABORATORY INVESTIGATION
(2023)
Meeting Abstract
Medicine, Research & Experimental
Karen Nahmod, Sa Wang, Shaoying Li, Zhenya Tang, Fnu Aakash, Jie Xu, Shimin Hu, L. Jeffrey Medeiros, Guilin Tang
LABORATORY INVESTIGATION
(2023)
Article
Pathology
Juliana Mota Siqueira, Yoshitsugu Mitani, Camilla Oliveira Hoff, Flavia Bonini, Luana Guimaraes de Sousa, Mario L. Marques-Piubelli, Anurag Purushothaman, Mutsumi Mitani, Hui Dai, Shiaw-Yih Lin, Michael T. Spiotto, Ehab Y. Hanna, Daniel J. McGrail, Adel K. El-Naggar, Renata Ferrarotto
Summary: B7-H4 expression pattern varies among different types of salivary gland carcinomas, and high B7-H4 expression is associated with poor prognosis in adenoid cystic carcinoma.
Article
Pathology
Basile Tessier-Cloutier, Felix K. F. Kommoss, David L. Kolin, Kristyna Nemejcova, Dupreez Smith, Jennifer Pors, Colin J. R. Stewart, W. Glenn Mccluggage, William D. Foulkes, Andreas von Deimling, Martin Kobel, Cheng-Han Lee
Summary: This study provides a detailed analysis of the clinical, pathological, immunohistochemical, and molecular features of DDOC/UDOC. The majority of patients presented with extraovarian disease and had rapid disease progression resulting in high mortality rate.
Review
Pathology
Sophia J. Wagner, Christian Matek, Sayedali Shetab Boushehri, Melanie Boxberg, Lorenz Lamm, Ario Sada, Dominik J. E. Winter, Carsten Marr, Tingying Peng
Summary: Computational pathology research driven by deep learning faces challenges in reproducibility and reusability. Codebase with good documentation and robustness and generalizability of models are crucial. The reuse of computational pathology algorithms is limited, and their application in clinical settings is even rarer. This study evaluates 160 peer-reviewed articles, providing criteria for data and code availability and statistical analysis of results.
Article
Pathology
Andres M. Acosta, Lynette M. Sholl, Fiona Maclean, Chia-Sui Kao, Thomas M. Ulbright
Summary: This study assessed the clinicopathologic and genomic features of 14 cases of testicular sex cord-stromal tumors. The results showed that CTNNB1 mutations are rare in these tumors, and most of them have genomic alterations similar to testicular sex cord-stromal tumors with pure or predominant spindle cell components.
Article
Pathology
Toru Odate, Kaishi Satomi, Takashi Kubo, Yuko Matsushita, Toshihide Ueno, Akira Kurose, Kohei Shomori, Tokiko Nakai, Reiko Watanabe, Keiko Segawa, Shusa Ohshika, Naritomo Miyake, Sayaka Kudo, Tatsunori Shimoi, Eisuke Kobayashi, Motokiyo Komiyama, Seiichi Yoshimoto, Fumihiko Nakatani, Akira Kawai, Yasushi Yatabe, Shinji Kohsaka, Koichi Ichimura, Hitoshi Ichikawa, Akihiko Yoshida
Summary: Inflammatory rhabdomyoblastic tumors (IRMTs) are newly recognized skeletal muscle tumors with uncertain malignant potential. This study investigated 13 IRMTs using clinicopathologic, genetic, and epigenetic methods. The results showed specific histologic features and genetic mutations in these tumors, and most of them exhibited benign behavior.
Article
Pathology
Dale L. Davis, Adam C. Lechner, David B. Chapel, Jonathan C. Slack, Chrystalle Katte Carreon, Bradley J. Quade, Carlos Parra-Herran
Summary: The Amsterdam Consensus Statement introduced the term maternal vascular malperfusion (MVM) to classify a group of findings related to impaired maternal-placental circulation. The study found that features such as low placental weight, accelerated villous maturation, decidual arteriopathy, and infarcts are associated with adverse obstetrical outcomes, while the role of other features like distal villous hypoplasia, excess multinucleated trophoblast, and retroplacental hemorrhage needs further research.
Review
Pathology
Alain C. Borczuk
Summary: COVID-19 is an acute respiratory illness that can progress to acute respiratory distress syndrome. While most patients recover completely, some may experience persistent respiratory dysfunction, known as long COVID. The pathogenesis involves immune and cellular disturbances.
Article
Pathology
Annikka Weissferdt, Cheuk H. Leung, Heather Lin, Boris Sepesi, William N. William, Stephen G. Swisher, Tina Cascone, J. Jack Lee, Abujiang Pataer
Summary: Neoadjuvant treatment of non-small cell lung cancer challenges traditional processing of pathology specimens, and accurate evaluation of residual tumor is crucial for assessing treatment efficacy.
