Journal
MITOCHONDRION
Volume 13, Issue 5, Pages 507-514Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.mito.2012.10.005
Keywords
Oxygen-dependent quenching; Protoporphyrin IX; Delayed fluorescence; Oxygen disappearance rate; Phosphorescence quenching; Tissue oxygenation
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Funding
- Netherlands Organization for Health Research and Development (ZonMW) [40-41300-98-9037]
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Progress in diagnosis and treatment of mitochondrial dysfunction in chronic and acute disease could greatly benefit from techniques for monitoring of mitochondrial function in vivo. In this study we demonstrate the feasibility of in vivo respirometry in skin. Mitochondrial oxygen measurements by means of oxygen-dependent delayed fluorescence of protoporphyrin IX are shown to provide a robust basis for measurement of local oxygen disappearance rate (ODR). The fundamental principles behind the technology are described, together with an analysis method for retrievel of respirometry data. The feasibility and reproducibility of this clinically useful approach are demonstrated in a series of rats. (C) 2012 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
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