Journal
MITOCHONDRION
Volume 8, Issue 3, Pages 237-246Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.mito.2008.04.003
Keywords
10-N-alkyl acridine orange; cardiolipin; mitochondria; NAO analogues
Categories
Funding
- NCI NIH HHS [P30 CA043703-17, R01 CA106491-04, R01 CA106491-01A1, P30 CA043703-18, R01 CA106491-02, R01 CA106491, P30 CA043703-16, P30 CA043703, P30 CA43703, R01 CA106491-03] Funding Source: Medline
Ask authors/readers for more resources
10-N-Nonyl acridine orange (NAO) is used as a mitochondrial probe because of its high affinity for cardiolipin (CL). Targeting of NAO may also depend on mitochondrial membrane potential. As the nonyl group has been considered essential for targeting, a systematic study of alkyl chain length was undertaken; three analogues (10-methyl-, 10-hexyl-, and 10-hexadecyl-acridine orange) were synthesized and their properties studied in phospholipid monolayers and breast cancer cells. The shortest and longest alkyl chains reduced targeting, whereas the hexyl group was superior to the nonyl group, allowing very clear and specific targeting to mitochondria at concentrations of 20-100 nM, where no evidence of toxicity was apparent. Additional studies in wild-type and cardiolipin-deficient yeast cells suggested that cellular binding was not absolutely dependent upon cardiolipin. (c) 2008 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available